With the rising prevalence of methicillin-resistant Staphylococcus ( S .) aureus (MRSA), a multi drug resistant strain, treatment has become a challenge. The infection frequently involves the skin and soft tissue, but it can also cause other life-threatening systemic illnesses including endocarditis, meningitis, and toxic shock syndrome. The known antibiotic resistance determinants, mecA and mecC , detected in MRSA isolates exhibit a broad spectrum of cross-antibiotic resistance. This study identified three functional protein families, through sequencing, that confer extensive antibiotic resistance independent of mecA and mecC . Twenty-six isolates had their DNA sequenced for mepR , mepA , mepB , and sapep genes for correlation with their antimicrobial phenotypes. Transcriptional profiling revealed that both animal and human MRSA isolates exhibited a gene cluster mepRAB (multidrug export protein gene), encoding a MarR-like transcriptional regulator ( mepR ), a M20/M25/M40 metallo-hydrolase protein gene ( sapep ) encoding resistance to biocides and carbapenems, and a hypothetical protein gene of unknown function ( mepB ).
The work entitled “ Genetic resistance factors and antimicrobial resistance phenotypes in methicillin-resistant Staphylococcus aureus isolates of animals and humans ” was published on One Health Bulletin (published on Jan. 23, 2025).
One Health Bulletin
Experimental study
Animals
Genetic resistance factors and antimicrobial resistance phenotypes in methicillin- resistant Staphylococcus aureus isolates of animals and humans
23-Jan-2025