Up until now, however, technical difficulties have hampered the development of such tools; the targets for these molecules are often folded extensively, making particular cleavage sites inaccessible to the catalyst. However, in the May 15 issue of EMBO reports, H. Kawasaki and K. Taira report on a technical breakthrough. By linking ribozymes to helicases, cellular components whose normal function is to ‘smooth out’ folded RNA’s to allow them to be ‘translated’ into proteins, these investigators have managed to circumvent this ‘folding’ difficulty. They have been able to efficiently inhibit the activities of a number of target RNA’s, even at sites that are known to be inaccessible to regular ribozymes. This has further allowed them to develop a method for investigating the functions of random RNA’s, creating a tool that may be invaluable in characterizing the functions of many of the previously unknown genes that have only recently been uncovered by various genome projects. Although we are not yet ready to treat any diseases using ribozymes, this study may indeed be a big step in the right direction.
EMBO Reports