Spatiotemporal characterization of disease-associated neurons in the entorhinal cortex-hippocampal circuit during Alzheimer’s disease progression

This study constructs a spatiotemporal single-nucleus transcriptomic atlas of neurons in the entorhinal cortex–hippocampal (EC-HPC) circuit during Alzheimer’s disease (AD) progression. By performing Smart-seq2-based single-nucleus RNA sequencing on neurons from APP/PS1 transgenic mice and wild-type controls across different brain regions and disease stages, the study reveals two distinct neuronal populations associated with AD pathology: progressively lost EC-stellate neurons and expanding GFAP⁺ neurons with glia-like features. These findings highlight neuronal identity changes and energy metabolism dysfunction in AD, offering new insights into early diagnosis and intervention.

Protein & Cell

10.1093/procel/pwaf042

Experimental study

Not applicable

Spatiotemporal characterization of disease-associated neurons in the entorhinal cortex-hippocampal circuit during Alzheimer’s disease progression

10-Jun-2025