Thrombosis, a leading global cause of death, often results from blood clots forming inappropriately in arteries or veins. While fibrinogen—a vital blood plasma protein—is well-known for its role in clot formation, this new study led by Prof. Matteo Becatti and his research team delves into how post-translational modifications (PTMs) can alter its structure and function. The findings, recently published in Molecular Biomedicine , open new avenues for understanding thrombotic disorders and developing advanced treatment strategies.
Fibrinogen is a large, hexameric glycoprotein produced in the liver. Its primary role is in hemostasis, where it is converted into fibrin to form the structural basis of blood clots at injury sites. Beyond its structural role, fibrinogen interacts with platelets and inflammatory mediators, linking coagulation with immune responses. However, fibrinogen is also highly susceptible to PTMs—chemical alterations after protein synthesis—that can dramatically impact its behavior. The research group led by Professor Becatti & Prof. Claudia Fiorillo is internationally recognized for its leadership in studying oxidative modifications of fibrinogen and their profound effects on thrombosis.
The study explores the most common PTMs, including oxidation, nitration, glycation, and phosphorylation, as well as less understood modifications like citrullination and homocysteinylation. These PTMs influence clot structure, density, and stability, potentially driving pathological conditions like thrombosis or bleeding disorders.
Key highlights from the study include:
Clinical Implications
Understanding the mechanisms behind fibrinogen PTMs has profound implications for managing thrombotic diseases. For example, the research sheds light on why individuals with conditions like diabetes or inflammatory disorders face higher risks of thrombotic complications. It also identifies potential molecular targets for drug development, paving the way for more precise and personalized therapies.
Future Directions
The study emphasizes the need for further exploration into less-characterized PTMs and their clinical significance. Advanced analytical techniques, such as mass spectrometry and fluorescence spectroscopy, are essential tools for unraveling the complex effects of these modifications.
The findings also call for more translational research to bridge the gap between molecular studies and clinical applications, particularly in developing therapeutic agents that counteract the negative impacts of fibrinogen modifications.
Prof. Becatti and his team hope their work will inspire broader research efforts in this area, ultimately improving outcomes for patients with thrombotic or bleeding disorders.
Molecular Biomedicine
Post-translational modifications of fibrinogen: implications for clotting, fibrin structure and degradation.
31-Oct-2024