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Universal amplification-free RNA detection with CRISPR-Cas10 and graphene field-effect transistors

07.02.25 | Shanghai Jiao Tong University Journal Center

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Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor
A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET).Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairpin DNA reporters on the GFET channel enable the detection limit to reach the level of aM.A universal sensing detection platform is established to directly detect the medium-length RNAs and miRNAs in clinical samp Credit: Mingyuan Sun, Zhenxiao Yu, Shuai Wang, Jiaoyan Qiu, Yuzhen Huang, Xiaoshuang Chen, Yunhong Zhang, Chao Wang, Xue Zhang, Yanbo Liang, Hong Liu, Qunxin She, Yu Zhang, Lin Han.

Researchers from Shandong University have developed a novel biosensing platform that integrates the CRISPR-Cas10 system with graphene field-effect transistors (GFETs) for amplification-free, highly sensitive, and specific RNA and microRNA (miRNA) detection. This innovative approach achieves detection limits at the attomolar (aM) level, making it a powerful tool for health monitoring and disease diagnosis.

Why the CRISPR-GFET Biosensor Matters

Innovative Design and Mechanisms

Future Outlook

Stay tuned for more groundbreaking advancements from the research team at Shandong University as they continue to explore innovative solutions for nucleic acid detection and molecular diagnostics!

Nano-Micro Letters

10.1007/s40820-025-01730-3

Experimental study

Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor

30-Apr-2025

Keywords

Transistors

Article Information

Journal
Nano-Micro Letters
DOI
10.1007/s40820-025-01730-3
Method of Research
Experimental study
Article Publication Date
2025-04-30
Article Title
Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor

Contact Information

Bowen Li
Shanghai Jiao Tong University Journal Center
qkzx@sjtu.edu.cn

Source

Original Source
https://link.springer.com/article/10.1007/s40820-025-01730-3

How to Cite This Article

APA:
Shanghai Jiao Tong University Journal Center. (2025, July 2). Universal amplification-free RNA detection with CRISPR-Cas10 and graphene field-effect transistors. Brightsurf News. https://www.brightsurf.com/news/L3R7ONE8/universal-amplification-free-rna-detection-with-crispr-cas10-and-graphene-field-effect-transistors.html
MLA:
"Universal amplification-free RNA detection with CRISPR-Cas10 and graphene field-effect transistors." Brightsurf News, Jul. 2 2025, https://www.brightsurf.com/news/L3R7ONE8/universal-amplification-free-rna-detection-with-crispr-cas10-and-graphene-field-effect-transistors.html.