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A novel mechanism for overexpression of c-Myc, a proto-oncogene in cancer cells

02.28.22 | Nagoya City University

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Figure 2
USP17 regulates c-Myc-dependent cell proliferation and glycolysis. A. PC-3 cells and PC-3/FLAG-c-Myc cells were transiently transfected with the indicated siRNAs. After 72 h, cell viability was measured by a WST-8 cell proliferation assay. Results are shown as means ± S.D. (n=3). B, C. The medium from the PC-3 cell culture or PC-3/FLAG-c-Myc cell culture was collected for the analysis of glucose consumption (B) and lactate production (C). Results are shown as means ± S.D. (n=3). D. In cancer Credit: Nagoya City University

The c-Myc oncoprotein is frequently overexpressed in human cancers and is essential for cancer cell proliferation. The dysregulation of ubiquitin-proteasome-mediated degradation is one of the contributing factors to the up-regulated expression of c-Myc in human cancers.
In the present study, Dr. Hidetoshi Hayashi (Professor, Nagoya City University) and collaborators herein identified USP17 as a novel deubiquitinating enzyme that regulates c-Myc levels and controls cell proliferation and glycolysis. The overexpression of USP17 stabilized the c-Myc protein by promoting its deubiquitination. In contrast, the knockdown of USP17 promoted c-Myc degradation and reduced c-Myc levels. The knockdown of USP17 also suppressed cell proliferation and glycolysis. Collectively, the present results reveal a novel role for USP17 in the regulation of c-Myc stability, and suggest its potential as a therapeutic target for cancer treatment.

FEBS Letters

10.1002/1873-3468.14296

Experimental study

Cells

The deubiquitinating enzyme USP17 regulates c-Myc levels and controls cell proliferation and glycolysis

28-Feb-2022

Additional Media

Figure1
USP17 deubiquitinates to stabilize the c-Myc protein. A. 3HA-c-Myc was co-expressed with FLAG-USP17 (WT or the catalytically inactive mutant (C89S)) in COS7 cells. After 24 h, cell lysates were immunoblotted with the indicated antibodies. CS, C89S. B. PC-3 cells were transiently transfected with the indicated siRNAs. After 72 h, an immunoblot analysis was performed with the indicated antibodies. Ctrl, control. C. An empty vector or plasmid expressing USP17 WT or C89S was co-expressed with 3H Credit: Nagoya City University

Keywords

Biochemistry Cancer Cell Biology Health and Medicine Molecular Biology

Article Information

Journal
FEBS Letters
DOI
10.1002/1873-3468.14296
Method of Research
Experimental study
Subject of Research
Cells
Article Publication Date
2022-02-28
Article Title
The deubiquitinating enzyme USP17 regulates c-Myc levels and controls cell proliferation and glycolysis

Contact Information

Sasaki Tatsuro
Nagoya City University
ncu_public@sec.nagoya-cu.ac.jp

How to Cite This Article

APA:
Nagoya City University. (2022, February 28). A novel mechanism for overexpression of c-Myc, a proto-oncogene in cancer cells. Brightsurf News. https://www.brightsurf.com/news/L59J59V8/a-novel-mechanism-for-overexpression-of-c-myc-a-proto-oncogene-in-cancer-cells.html
MLA:
"A novel mechanism for overexpression of c-Myc, a proto-oncogene in cancer cells." Brightsurf News, Feb. 28 2022, https://www.brightsurf.com/news/L59J59V8/a-novel-mechanism-for-overexpression-of-c-myc-a-proto-oncogene-in-cancer-cells.html.