A comprehensive investigation by KAUST researchers sets the record straight on the formation of hydrogen peroxide in micrometre-sized water droplets, or microdroplets, and shows that ozone is the key to this transformation 1 , 2 .
The air-water interface is a crucial site for numerous natural, domestic and industrial processes such as ocean-atmosphere exchange, cloud and dew formation, aerated beverages and bioreactors. Yet, probing chemical transformations at the air-water interface is challenging due to the lack of surface-specific techniques or computational models.
In collaboration with Ph.D. students Adair Gallo Jr and Nayara Musskopf, research scientist Peng Zhang utilized an ultrasensitive fluorescence-based assay that can detect aqueous H 2 O 2 with an almost 40-times lower limit of detection than the original assay. They found no H 2 O 2 in condensed microdroplets from hot water, but up to one micromolar H 2 O 2 in microdroplets from commercial ultrasonic humidifiers. With this key insight, the team investigated sprays.
Computer simulations by postdoc Xinlei Liu and high-speed imaging by Ph.D. student Ziqiang Yang demonstrated that bowl-shaped shock waves formed in sprays, but these conditions were inadequate to chemically transform water into H 2 O 2 .
“Questions remained: where did the rest of the H 2 O 2 come from in the condensed and sprayed microdroplets investigated in California, and why didn’t we see it at KAUST?”, says lead author Gallo Jr. After several failed attempts to explain the conundrum, the team turned to ambient ozone as a potential actor in H 2 O 2 formation. “I had a Eureka moment while reading papers from 40 years ago. They had listed ambient ozone as an interferent in aqueous H 2 O 2 measurements", explains Mishra.
To control ambient ozone levels, the researchers used an ozone generator and mixed the resulting gas with nitrogen gas before introducing it into a glovebox. They observed that increasing the ozone concentration enhanced H 2 O 2 formation. “We were so happy because this was the answer,” says co-lead author Musskopf.
While ambient ozone concentrations remain below 2 parts per billion inside our glovebox, they can exceed 80 parts per billion in California, according to records collected by the Environmental Protection Agency. Although ozone minimally dissolves in water, the enhanced surface area of microdroplets allows more ozone to be dissolved and quickly react to form H 2 O 2 . “There had to be something related to the geography of the place, an environmental difference between our location in Saudi Arabia and California,” Gallo Jr says.
Together, these data disprove that water spontaneously transforms into H 2 O 2 at the air-water interface. “We have defended textbook physical chemistry and what we know about water”, Mishra concludes.
Chemical Science
Experimental study
On the formation of hydrogen peroxide in water microdroplets
14-Jan-2022