Plant-based alternatives to yoghurt are often made using bacterial cultures employed in yoghurt production, even though plant-based raw materials differ markedly from milk. For example, there is no lactose in plants, and plant proteins are more difficult to break down than milk proteins.
Researchers from DTU, in collaboration with colleagues at Chalmers University of Technology and the University of Cambridge, therefore investigated whether lactic acid bacteria isolated from vegetables and fermented foods, and often found in the intestines of humans and animals, might be better suited to the task.
The research, which has been published in the Journal of Food Protection , showed that three plant-isolated enterococci (a type of lactic acid bacteria) were highly suitable for producing a soy-based yoghurt alternative.
“The bacteria did more than simply ferment the soy drink. They were also able to suppress undesirable bacteria, break down unwanted sugars, and reduce phytic acid, that make it difficult for the body to absorb minerals such as iron, zinc, magnesium and calcium,” says postdoctoral researcher Belay Tilahun Tadesse of the DTU National Food Institute.
In the experiments, the lactic acid bacteria grew efficiently in the soy drink and lowered the pH, making the product acidic and yoghurt-like. The most promising candidates acidified rapidly, even without the addition of sugar.
The bacteria were also able to ferment at a relatively high temperature, at which many undesirable microorganisms, including pathogenic ones, do not thrive. This could help to extend shelf life and improve food safety.
“We observed that three of the bacterial strains quickly took control of the fermentation in the soy drink. This is important because stable and effective acidification is one of the first requirements when developing a robust product,” says Belay Tilahun Tadesse.
The three most promising bacterial strains showed a broad antimicrobial effect against pathogenic bacteria, including listeria and E. coli. This makes them particularly interesting, given the high priority placed on food safety.
In addition, the bacterial strains tested were able to produce compounds that contribute to texture, making the yoghurt creamier.
Overall, the research findings show that the isolated enterococci have a stronger effect against undesirable microorganisms and a better acidification capacity than enterococci already on the market and approved for use in food and feed.
“We have shown that the lactic acid bacteria tested are safe. However, further research is needed, for example, to determine how flavour is affected and whether plant raw materials other than soy can be used. Also, before they can be used in Europe, they must be approved by the European Food Safety Authority (EFSA),” says Associate Professor Christian Solem of the DTU National Food Institute.
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The study was published in the Journal of Food Protection under the title Exploring the use of Safety-Assessed Bacteriocin-Producing Enterococci as Starters for Production of Soy Yoghurt Analogues .
The research was supported by a DTU Alliance PhD/Research Fellowship, the Novo Nordisk Foundation, and a Shuttleworth Foundation Fellowship.
Read about The Research Group for Microbial Biotechnology and Biorefining .
Journal of Food Protection
Exploring the use of Safety-Assessed Bacteriocin-Producing Enterococci as Starters for Production of Soy Yoghurt Analogues
14-Feb-2026
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.