Unlike what we commonly refer to as 'genes', these phantom genes or 'Long noncoding RNA' (LncRNAs) do not lead to the production of proteins that our cells, and thus our entire bodies are made of.
Previously, it was believed that LncRNAs served no major purpose in cells, but new research now shows that one of these LncRNAs termed 'LincIRS2' is important for safeguarding our metabolism as LincIRS2 loss favors development of metabolic complications in mice.
Editing mice with CRISPR
Using the 'molecular scissor' CRISPR/Cas9, Jan-Wilhelm Kornfelds research team succeeded in cutting out LincIRS2 from the mouse's genome. Next, the researchers observed that mice lacking LincIRS2 developed metabolic complications like elevated blood sugar levels when the LncRNA had been deactivated. Conversely, when performing treatments that activate LincIRS2, mice maintained healthy blood sugar levels even when becoming obese.
His research team just published these new findings in the prestigious journal Nature Communications . The lead author of the article is Dr. Marta Pradas-Juni, who is a postdoc in Jan-Wilhelm Kornfelds' research team.
Fact: What is a Long noncoding RNA?
DNA serves as blueprint for producing proteins that constitute the essential building blocks all cells are made of. The molecular intermediary that converts DNA information into proteins is called RNA. Thus, RNA's primary purpose is to translate the 'genes' DNA into protein.
In our bodies, 20,344 different genes are specifically designed to create the many different proteins that our bodies require. The majority of these so-called protein-coding RNAs have been mapped by scientists. That is why, we today largely understand exactly which proteins these RNAs give rise to.
However, nearly 60,000 RNAs called 'Long noncoding RNAs' are written into our genomes that never contribute to the formation of a protein. How they function, and how they are involved in disease development is largely unknown.
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Meet the researcher:
Jan-Wilhelm Kornfeld is DDA professor for Molecular Biology of Metabolic Diseases at the Department for Biochemistry and Molecular Biology at University of Southern Denmark. The research group of Jan-Wilhelm Kornfelds' lab uses modern genomic techniques like next-generation sequencing and CRISPR/Cas9 mouse models to better understand the biology of LncRNAs in metabolic disease. Their goal is to define novel RNA-based approaches for treatment of obesity and type 2 diabetes. Their research is supported by the European Research Council (ERC) and the Novo Nordisk Foundation.
Nature Communications