Cyclophosphamide (CP) is used directly or through its metabolites (phosphoramide mustard) to treat a variety of tumors. However, it has adverse effects. Lepidium draba L. ( L. draba ) has been used to treat many diseases. The current study aimed to examine the protective effects of L. draba on the liver and kidney in rats of CP-induced toxicity.
A total of 36 rats were divided into six groups as follows: the sham control group, the CP group (CP 100 mg/kg i.p. on days 1, 7, 14, 21, 28, and 35), the CP groups treated with L. draba extract (100, 200 and 400 mg/kg of L. draba extract for 28 d), and the L. draba extract alone group (400 mg/kg of L. draba extract for 28 d). Serum parameters of renal and hepatic function, as well as pro-inflammatory and anti-inflammatory cytokines associated with liver and kidney damage were measured. Moreover, Bax , Bcl-2 , and caspase-3 gene expression and histopathological changes were assessed.
L. draba extract alleviated CP-induced hepatotoxicity and nephrotoxicity by decreasing nitric oxide, TBARS, IL-6, TNF-α, and IL-1β levels, as well as increasing superoxide dismutase, catalase and glutathione peroxidase activities, and FRAP, MIF, and TGF-β levels. In addition, the extract downregulated the expression of pro-apoptotic genes ( Bax and caspase-3 ) and mitigated the destruction of glomeruli and renal tubules as well as the degeneration of hepatocytes.
L. draba extract can protect hepatic and renal structure and function against CP-induced toxicities, and may be used as a therapeutic agent for CP-induced hepatotoxicity and nephrotoxicity.
The work entitled “ Hepatorenoprotective effects of Lepidium draba L. extracts against cyclophosphamide-induced oxidative injuries in rats via reducing apoptosis and inflammation ” was published on Asian Pacific Journal of Tropical Biomedicine (published on Feb. 27, 2025).
DOI： 10.4103/apjtb.apjtb_518_24
Asian Pacific Journal of Tropical Biomedicine
Experimental study
Animals
Hepatorenoprotective effects of Lepidium draba L. extracts against cyclophosphamide-induced oxidative injuries in rats via reducing apoptosis and inflammation
27-Feb-2025