Articles tagged with Fluorescent Proteins
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
Scientists have created a way to track brain diseases like depression, Alzheimer's, and strokes using fluorescent mouse blood. The method allows for months-long study of blood flow in the brain, providing new insights into disease progression and development.
Researchers at KTH Royal Institute of Technology developed a method to track the growth of the HIV virus by highlighting its essential molecules. This breakthrough could lead to the creation of new therapies that target these molecules to prevent viral growth.
A novel 937-nm laser source has been developed for multiphoton microscopy, enabling deep tissue imaging at depths of over 600 µm with only 10 mW of power. This breakthrough technology offers a good balance between sensitivity, penetration depth, and imaging speed.
Researchers developed a novel way to visualize densely packed molecules using expansion microscopy, allowing for the first time their imaging. The technique enables visualization of nanostructures found in neurons and Alzheimer's-linked amyloid beta plaques.
Researchers at Weill Cornell Medicine found that disrupting circadian clocks leads to increased fat cell growth and insulin production. Stress and other factors can throw the body's 'clocks' out of rhythm, contributing to weight gain.
The Biofinder instrument has successfully detected bio-residue in ancient fish fossils from the Green River formation, confirming that biological residues can survive millions of years. The device's capabilities make it an ideal tool for future NASA missions to detect signs of past life on other planetary bodies.
Researchers have developed a new probe to detect Alzheimer's disease biomarkers using near-infrared fluorescence, which may help diagnose the disease early and prevent its progression. The probe binds oligomeric Aβ proteins, a hallmark of Alzheimer's disease, offering a potential alternative to existing treatments.
Researchers created edible tags with fluorescent silk proteins to track medications, providing a new way for consumers to verify authenticity. The codes are readable by a smartphone app and can be ingested without causing harm.
Researchers developed a full-function bioelectronic photocell using genetically modified proteins attached to a carbon nanotube. The system can change its electronic properties in response to light, operating as a spotlight or memory cell. This discovery opens the door to environmentally friendly electronic elements, memory devices, an...
Researchers at Bielefeld University have identified five key characteristics of mitosis in the microalga Volvox carteri, including a porous nuclear envelope and crucial centrosome function. They used confocal laser scanning microscopy to capture high-resolution images of live cell division and gain insights into the complex process.
A new tissue expansion method, eMAP, has been developed to improve neural imaging. It allows for the imaging of proteins at neural connections, enabling the measurement of their relative distances and abundance. The technology facilitates high-throughput analysis and enables multiscale imaging of synapses along whole neuronal branches.
A new study published in Developmental Cell reveals the mechanism of membrane curvature that allows cells to form pockets to capture substances. The researchers used high-resolution fluorescence imaging to watch these pockets form within live cells, providing a clearer understanding of how cells 'eat' and consume substances.
The study uses a new barcode system to track complex signaling activities in cancer cells and identify key protein interactions. The technique enables real-time analysis and synchronization of protein activity over time.
Researchers at the University of Tsukuba discovered a male-biased protein expression in primordial germ cells of fruit flies. The study used the Gal4-UAS system to induce gene expression and found that male cells had more protein synthesis occurring, with stronger GFP expression.
Researchers have used advanced microscopy to study the ultrastructure of huntingtin inclusions, revealing different mechanisms of aggregation that lead to distinct biochemical properties. The findings suggest targeting inclusion growth as a potential therapeutic strategy for slowing Huntington's disease progression.
Researchers at Texas Biomedical Research Institute developed 'reporter viruses' that allow for real-time tracking of SARS-CoV-2 spread in cells and animal models. This enables faster screening of potential anti-viral drugs, vaccines, and neutralizing antibodies.
Scientists have created a system dubbed "NanoporeTERs" allowing cells to express themselves in a whole new light. These new reporter proteins can detect multiple protein expression levels and shed new light on biological systems, enabling deeper analysis than before.
Researchers from Nara Institute of Science and Technology developed a machine learning program that accurately predicts the location of proteins related to actin in cells. The program achieved a high degree of similarity with actual images, showing promise for future applications in cell analysis and artificial cell staining.
Researchers discovered that fluorescent proteins behave like miniature antennas, absorbing and emitting light in specific directions. This finding has significant applications in basic biological research and novel drug discovery.
Researchers develop Single-residue Terminal Labeling (STELLA) to fluorescently tag microproteins without disrupting their function. The method enables visualization of elusive polypeptides from SARS-CoV2 coronavirus, opening new avenues for studying tiny proteins in living cells.
A new method called SPOTlight allows for the isolation of single live cells with unique profiles from heterogenous populations. The platform uses a digital micromirror device to give individual cells a long-lasting tag, enabling researchers to observe cellular dynamics and subcellular structures over time.
Scientists at Nagoya University developed a new method for visualizing microtubule dynamics and cell membrane protein endocytosis in living plant cells. They successfully used SNAP-tag to mark auxin transporters, allowing clear differentiation between newly synthesized and endocytosed proteins.
Researchers are developing bacterial proteins to create new, artificial fluorescent compounds. The goal is to produce biodegradable and sustainable light-emitting diodes (LEDs) using these novel compounds. By harnessing the power of bacteria, scientists hope to create more eco-friendly lighting solutions with minimal environmental impact.
Researchers at Purdue University have developed an edible 'security tag' to protect prescription drugs from counterfeiting. The tag uses a digital fingerprint and can be scanned by a smartphone app to verify authenticity.
Researchers at Michigan State University have created a synthetic nano-sized factory using a new shell protein that can be controlled to direct useful enzymes inside. The team successfully produced factory shells with the new protein, allowing them to incorporate molecules and control cargo imports.
A new study from KAUST has improved the efficiency of protein-induced fluorescence enhancement (PIFE) by identifying conditions that lead to either enhanced or quenched fluorescence. By understanding these conditions, researchers can better interpret laboratory results and gain more precise insights into molecular events.
A new frankenbody tool has been developed to enable live-cell imaging, using a genetically encoded probe that binds to specific targets. This probe offers a cost-effective alternative to traditional fluorescent protein tags, allowing for real-time visualization of protein dynamics and RNA translation in living cells.
A new antibody-based probe, called a frankenbody, enables live-cell imaging of proteins by binding to specific targets, like the classic HA tag. This tool allows for real-time visualization of protein dynamics and has applications in RNA translation studies, providing a low-cost solution.
Researchers have engineered a new fluorescent protein that glows under UV and blue light, is thermally stable, and can emit light in the absence of oxygen. This breakthrough resolves previous limitations of fluorescence microscopy, enabling scientists to study living tissue more effectively.
BioBits Health, a Northwestern University-led project, introduces CRISPR and antibiotic resistance to high school students. Students perform experiments using freeze-dried cell-free reactions, visualizing DNA editing and drug resistance, and exploring ethics.
Researchers successfully created self-assembled structures using oppositely charged synthetic proteins, enabling the formation of hierarchical ordered, symmetrical structures. This breakthrough could lead to the development of novel architectures for bio-enabled sensing and functional coatings with unique properties.
Researchers designed proteins that can assemble into complex structures using supercomputers and artificial charges. The stacked octamer structure consists of 16 proteins, resembling a braided ring with highly ordered and specific interactions.
Researchers developed a method using fluorescent compound AggTag to identify intermediate forms of proteins that misfold and aggregate in live cells, which are believed to contribute to neurodegenerative diseases such as Alzheimer's and Parkinson's. The method allows for simultaneous detection of multiple proteins with distinct colors.
Researchers at IDIBELL have developed Nested CRISPR, a cloning-free method for genome editing using long DNA fragments. The technique involves two steps: inserting a small portion of the fragment into the genome and then using it as a
Rosana Molina, a Montana State University doctoral candidate, received a three-year F31 fellowship grant to develop more efficient two-photon excitation methods for neuroscience research. This technology enables deeper brain imaging and has the potential to revolutionize our understanding of the brain.
Researchers have developed a new method to study the flu virus, allowing them to visualize individual proteins and understand how they contribute to the virus's success. The study suggests that variations in protein composition may be beneficial for the virus, enabling it to spread infection more effectively.
Researchers at Yale University have identified a new fluorescent protein, VARNAM, that allows for live neurons to glow red when activated, making it possible to monitor brain activity in a less invasive way. This breakthrough enables scientists to capture complex brain activity spikes in multiple systems.
Researchers developed switchable fluorescent proteins that can be controlled by green and orange light, enabling the study of dynamic processes in living cells without harming them. The proteins' efficient photoswitching allows for super-resolution fluorescence microscopy, a method previously hampered by toxic irradiation.
The TCS SP8 FALCON system enables fast fluorescence lifetime imaging and fluorescence correlation spectroscopy, allowing scientists to investigate protein environments with high accuracy. This technology facilitates advanced techniques in protein biology, including dynamic maps of protein interactions in living cells.
A research team at Max Delbrück Center identified tiny huntingtin protein fibers that precede larger deposits in Huntington's disease, enabling prediction of disease onset months in advance. These findings hold promise for diagnosis and potential new treatments by testing pharmaceutical substances against the fibers' harmful activity.
Researchers at Tokyo Institute of Technology developed a fluorescent protein sensor that can provide real-time information on dynamic changes in oxygen levels. The ANA sensor shows very high sensitivity in tracking changes in oxygen content.
Researchers have developed a novel technique using genetically-encoded glucose biosensors to monitor Trypanosoma brucei parasite metabolism and identify molecules that disrupt glucose levels. This could lead to the development of therapeutics for African sleeping sickness, a disease causing fatal results in sub-Saharan Africa.
Researchers have created a silk hybrid material that attacks bacteria when illuminated by green light, producing reactive oxygen species to break down organic contaminants. The biocompatible material could be used for wound healing and environmental remediation, including air and water purification.
Rice University researchers confirmed their theory on the mechanism behind a fluorescent biosensor that monitors neurons by sensing changes in voltage. They developed a method to test fluorescent biosensors using computer simulations, resolving a decade-long debate between scientists.
The Biophysical Society has announced the winners of its international travel grants, chosen based on scientific merit and proposed presentation at the meeting. The recipients will be honored at a reception on February 18, 2018, in San Francisco.
Researchers from MSU and Denmark have discovered the mechanism behind green fluorescent protein's sensitivity to light exposure. They found that an isolated chromophore group can emit light outside the protein environment, while the protein enhances its fluorescent properties.
Researchers at Nagoya University have developed a new photostable fluorescent dye, PhoxBright 430 (PB430), to visualize cellular ultrastructure. PB430 enables continuous STED imaging and can be used for multicolor imaging of biological structures.
Researchers developed a new tool called FLARE to label neurons during specific tasks, providing greater temporal precision than current cell-labeling techniques. This approach could offer significant insights into neuron function and be used to study learning and memory, emotions, and diseases like Alzheimer's.
Researchers at EMBL and ESPCI Paris have developed a new technique to rapidly sort HIV viruses, which could significantly speed up vaccine development. The system enables the analysis and sorting of hundreds of HIV viruses per second, allowing for rapid testing of millions of viral variants.
Researchers at Columbia University have developed a new optical microscopy platform with drastically enhanced detection sensitivity, allowing for simultaneous labeling and imaging of up to 24 specific biomolecules. This breakthrough has the potential to transform understanding of complex biological systems, including the human cell map...
CAS researchers have developed a new monomer fluorescent protein, Skylan-NS, enabling substantial improvements in the speed, duration, and noninvasiveness of live-cell superresolution microscopy. The protein shows high photostability, cycle numbers and signal-to-noise ratio, making it suitable for live-cell SR imaging.
Molecular microbiologists at UMass Amherst identify a distinct domain on the plasma membrane of Mycobacterium smegmatis, crucial for bacterial growth. The discovery provides insight into lipid metabolism and regulatory mechanisms in mycobacteria, potentially leading to new methods of inhibiting bacterial growth.
Researchers use light to measure the 'big stretch' in spider silk proteins, shedding light on biological events such as cancer metastasis. The tool allows for real-time measurement of forces acting on proteins in live cells.
The Journal of Biomedical Optics special section honors Osamu Shimomura's work on green fluorescent protein, enabling researchers to observe molecular-level activity in live cells. Recent studies detail new applications of protein photonics, including multicolor imaging and monitoring cellular magnesium levels.
Researchers at ETH Zurich have developed a new microscopy technique that enables selective visualization of individual cells within complex tissue. Using 'chameleon proteins' like Dendra 2, they can highlight single cells or groups of molecules with one color while keeping other cells visible in another color.
Researchers have developed a new fluorescent protein called CaMPARI, which permanently marks neurons that are active at a particular time. This allows scientists to visualize neural activity beyond the limited field of view of a microscope and capture snapshots of neural activity during complex behaviors.
Researchers have developed a new method for detecting and imaging protein-protein interactions in live cells using color changes, enabling immediate visualization of biochemical events. The FPX technique converts biochemical processes into dramatic green to red color changes.
Researchers discovered that using solid-state proteins instead of solution increases laser intensity, leveraging natural protein structures to optimize brightness. This breakthrough enables the development of efficient miniature solid-state lasers and potential biocompatible applications.
Researchers use photoactivatable complementary fluorescent proteins to observe and quantify protein-protein interactions in live cells with single molecule level precision. The technique reveals a surprisingly critical role for a previously uncharacterized EB1 linker region in tracking microtubule plus-ends.
Researchers at Helmholtz Munich have created a novel fluorescent marker that excites in the far-red spectrum and emits in the infrared range, enabling better-quality images with advanced bio-imaging. This technology allows for the delineation of tumor and metastasis, tracking drug responses within whole-body imaging.