Articles tagged with Fluorescent Proteins
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
Researchers at ICFO have developed a framework to quantify photoactivation efficiency of fluorescent proteins, enabling accurate protein stoichiometry measurement. This breakthrough enhances our ability to study protein function and disease mechanisms.
Researchers discovered a novel fluorescent protein in the freshwater eel that can bind to bilirubin, enabling high sensitivity and accuracy for clinical tests. This breakthrough may contribute to liver function assessment and eel conservation efforts.
Researchers engineered Chlamydomonas reinhardtii into a rainbow of colors by producing six different fluorescent proteins in the algae cells. This innovation provides a powerful tool for algae researchers to sort cells, view cellular structures, and create fusion proteins.
Researchers have developed a diatom-based biosensor that can detect specific substances in water samples using fluorescence. The biosensor uses genetic engineering to insert fluorescent proteins into the silica shell of a marine algae, allowing it to respond to certain chemicals.
Researchers have created a new cyan fluorescent protein (CFP) called mTurquoise2, which triples the fluorescence efficiency of existing proteins, enabling improved cellular imaging with unprecedented sensitivity. This breakthrough allows scientists to study protein-protein interactions in living cells with increased accuracy and detail.
A new method of monitoring protein molecules using gold nanoparticles has been developed by scientists at Johannes Gutenberg University Mainz. The technique allows for the detection of individual unlabeled proteins, providing insights into molecular processes and dynamics.
Researchers create specially engineered mammalian cells with a chemical handle to label proteins of interest efficiently without disrupting their function. The new approach enables fast, high-yield protein labeling and has advantages over existing methods.
Researchers at Albert Einstein College of Medicine have developed a new fluorescent protein, iRFP, that allows for the non-invasive visualization of internal organs in live animals. The protein absorbs and emits light in the near-infrared spectrum, enabling clear imaging without radiation exposure or contrast agents.
Scientists at TUM create customized fluorescent proteins in various colors for future applications by incorporating a genetically encoded non-natural amino acid into widely used natural proteins like GFP. The new bio-molecule exhibits a pseudo-Stokes shift, allowing it to be excited with commercially available black-light lamps.
Scientists have developed an instrument that tracks protein conformation and translocation with nanoscale precision. This breakthrough enables researchers to study proteins that regulate DNA replication and transcription, revealing new insights into their mechanisms.
The Biophysical Society has selected 2011 Fellows for their outstanding achievements in the field of biophysics, including advancements in molecular dynamics simulation and superresolution microscopy. The newly appointed Fellows will be honored at the Awards Ceremony during the annual meeting.
The development enhances fluoromodule technology by making probes glow five- to seven-times brighter than EGFP, allowing researchers to monitor biological activities in real-time. Dendron-based dyedrons amplify the signal emitted by fluoromodules, providing a single compact protein tag with signal enhancement.
Scientists have engineered a variant of fluorescent protein from reef coral to observe protein movement in live cells. The newly created mIrisFP has excellent properties as a genetically encoded marker protein, enabling the study of dynamical processes within live cells at high spatial resolution.
Researchers design a new technique called PRIME, which tags proteins with smaller probes allowing them to carry out normal functions. This breakthrough sheds light on previously unseen protein activities, offering new insights into cell biology.
Researchers successfully used a specialized fluorescent protein to visualize electrical activity in living mice, allowing them to study brain function and behavior in real-time. The 'cameleon' protein enables measurement of action potentials without electrodes, providing insights into neural networks and brain circuitry.
The study reveals the crystal structures of two key fluorescent proteins, blue and red, allowing for rational design of new proteins with specific colors. This breakthrough enables researchers to explore biological processes in normal cells versus cancer cells.
Researchers have observed dynamic behavior of allophycocyanin protein for over one second, a significant increase from previous methods, revealing new insights into its shape-changing dynamics.
Researchers have discovered a way to visualize iron-sulfur clusters in living cells using a custom protein tag, enabling analysis of diseases involving these metalloclusters. This technique has high potential for helping find real treatments for diseases such as Friedreich's ataxia and myopathy.
New live cell imaging techniques allow researchers to visualize dynamic changes in living cells, providing insights into biological processes. The application of computational image processing is also crucial for extracting meaningful data from this type of imaging.
New photoactivatable fluorescent proteins (PAFPs) and advanced fluorescent proteins (FPs) allow scientists to visualize individual cellular molecules in living cells. These tools are transforming biomedical research by enabling the study of cancer cells, protein-protein interactions, and cellular processes.
A team of scientists has discovered a new green fluorescent protein in a deep-sea creature, which can be used as a marker in living cells and tissues. The protein, named cerFP505, has similar brightness and stability to existing fluorescent proteins, making it an ideal lead structure for super-resolution microscopy.
Researchers successfully optically detected individual action potentials in brain cells of mice, enabling observation of brain activity over months. This new method provides insights into neural communication and may aid in identifying early onset of neurological disorders like Alzheimer's and Parkinson's.
A new technology developed at Yale allows researchers to detect and identify protein interactions within living cells without disrupting them. The method uses small molecule probes that bind to specific amino acid tags, enabling the visualization of protein conformations at high resolution.
Researchers at Harvard University have developed a new technique called Brainbow that allows for the imaging of neurons in a wide range of colors, enabling scientists to better map the complex wiring diagram of the brain and nervous system. This breakthrough has significant implications for understanding brain disorders and development.
Researchers at the University of Oregon have discovered the structural basis for photoswitching in fluorescent proteins, allowing for control over light emission. The study revealed that inserting a single oxygen atom can delay the switch-on time from five minutes to 65 hours, enabling more precise studies within cells.
A Scripps research study reveals the dynamic structural details of single prion molecules, shedding light on normal folding mechanisms and abnormal amyloid fibril conversion. The findings may lead to novel therapeutic targets for neurodegenerative diseases.
Researchers develop a fluorescent marker to distinguish between mitochondria in neurons and glial cells, providing insights into neurodegenerative diseases. The study sheds light on the role of mitochondrial dysfunction in aging and neurological disorders.
Invitrogen introduced its new free online scientific resource collection, iGene, allowing researchers to search for experimental reagents by gene or protein. The company also launched the Premo comeleon calcium sensor, which uses fluorescent protein color emission to detect calcium levels in live cells.
Researchers at Carnegie Institution for Science have developed a new technology to monitor glucose levels in leaf and root tissues of Arabidopsis thaliana, revealing extremely low sugar levels in roots. The breakthrough enables studies on sugar metabolism in plants and has potential applications for engineering higher crop yields.
UC San Diego scientists chart rapid progress in developing new fluorescent probes to study proteins in living cells. These techniques enable the localization of molecular machines in situ by electron microscopy.
Scientists have developed a new fluorescent tag called Dendra that allows for precise labeling and tracking of proteins in living cells. This innovation enables researchers to study protein and organelle dynamics, cell migration, inflammation, and other biological processes with unprecedented accuracy.
Researchers developed a new assay to observe real-time gene expression in live cells, providing unprecedented insights into fundamental biological processes. The technique detects protein molecules being produced in small bursts within cells and could reveal the randomness of gene expression.
Researchers used FRET and ALEX techniques to measure protein distances, shedding light on protein folding dynamics. The study aims to understand how proteins fold and unfold, holding key to Alzheimer's disease prevention and treatment.
Yale researchers have developed a method to count absolute numbers of individual protein molecules inside living cells and measure their locations with high accuracy. This breakthrough addresses fundamental hurdles for studying biology quantitatively, enabling the measurement of protein concentrations in various cellular structures.
Scientists have discovered how a biomolecule can act as a light switch, revealing its potential for high-resolution microscopy and optical data storage. The protein, asFP595, switches between fluorescent and non-fluorescent states using a tiny molecular mechanism.
Researchers discover crystal structure of cyan fluorescent protein, leading to understanding of coral reef coloration. The study provides insight into the biological function of coral reef coloration and its potential connection to environmental stresses.
A new tool developed by Carnegie Mellon University enables location proteomics by classifying and relating proteins inside cells. The tool outperforms existing methods using standardized features and automates clustering of proteins, allowing for fast and objective analysis.
Researchers used somatic hypermutation to evolve a red fluorescent protein with improved stability and color emission properties. The new protein, mPlum, was created by allowing B cells to mutate the gene at a rate of roughly a million times that of the genome. This process enabled the production of multiple mutations in a single cycle.
Scientists have developed a range of new fluorescent proteins with unique colors, allowing them to track the effects of multiple genetic alterations in a single cell. These monomeric proteins retain fluorescent properties while being less toxic than their multimeric counterparts, enabling precise cellular analysis.
Scientists have developed a new fluorescent protein probe to study cyclic AMP activity in living cells. The probe allows for real-time monitoring of cyclic AMP's impact on cellular responses, revealing its importance in various biological processes.
Fragile X Syndrome may be caused by altered nerve cell growth and connectivity. Neurons lacking the protein exhibit increased complexity, while those overexpressing it are simplified.
Researchers at U-M used a technology called ubiquitin-mediated fluorescence complementation to study a cell-signaling mechanism. They discovered how ubiquitin modified protein Jun's function and location, and found that an E3 ligase binding enzyme called Itch played a key role in this process.
Researchers will create fluorescent dyes to bind to DNA, lipids, carbohydrates, and proteins in Martian soil to detect life forms. The system aims to be versatile enough to couple with different types of rovers used in planetary expeditions.
A Harvard chemist has developed molecular mimics that rival the complexity of nature using innovative cell screening techniques. The approach involves attaching a natural protein to a fluorescent tag and then screening molecules for their ability to perturb cellular processes.
A new method for identifying specific proteins in blood or tissue samples uses protein microarrays to create a molecular 'portrait' of the sample. This technique has been shown to detect specific proteins in concentrations of less than one part per billion, with potential applications in clinical diagnosis and drug development.
Researchers have created protein microarrays that can measure the function of thousands of proteins, enabling rapid screening of small-molecule drug candidates and profiling of enzymes in cells. The technique preserves protein function and functionality, allowing for creation of 'protein snapshots' of cells.
Scientists at UCSF have shown that deformed prion proteins can trigger normal proteins to change shape and become infectious. The researchers used a new system to introduce prions into yeast, eliminating the possibility of non-prion molecules contributing to infection.
Researchers are working to create a better lung surfactant mixture that can be easily produced without batch variance, tailored to specific cases. The new formulation aims to reduce mortality rates by 30-50% for infants with neonatal respiratory distress syndrome.
UCSF researchers developed a highly sensitive, rapid technique for detecting infectious prions causing prion diseases like 'mad cow' disease and Creutzfeldt-Jakob's disease. The assay reveals unique shapes of the protein strains, providing new insights into their biology.
Researchers developed a genetically-engineered protein biosensor that can identify the presence of maltose, a sugar compound, in blood. The biosensor uses a fluorophore reporter to signal its catch, providing instant analysis for various applications.
Researchers at the University of Illinois have developed a fast measurement technique that sheds light on the early stages of protein folding. The initial steps of helix formation occur within several hundred nanoseconds, and the entire collapse to a compact structure appears nearly complete after just a few microseconds.
Researchers at Johns Hopkins University have made a surprising discovery about the movement of proteins within the Golgi apparatus. The enzymes, which are crucial for various cellular processes, were found to be mysteriously retained in the organelle despite their rapid movement, contradicting long-held assumptions about their function.
Scientists at the University of Chicago's Howard Hughes Medical Institute have discovered that an improperly folded protein in yeast cells can corrupt other proteins, leading to heritable changes. This finding supports the 'prion hypothesis' and provides direct evidence for protein-based inheritance.