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Zinc finger nuclease, immunoprecipitation methods featured in Cold Spring Harbor Protocols

A protocol for detecting targeted chromosomal deletions induced by zinc finger nucleases allows researchers to estimate the frequency of ZFN-induced genomic deletions using PCR-based methods. Immunoprecipitation is also optimized with detailed instructions for lysis conditions, protease inhibitor cocktails, and general preparation.

SourceCold Spring Harbor Laboratory·JournalCold Spring Harbor Protocols·DateAug 2, 2010