The researchers have investigated the efficacy of polo like kinase-1 (PLK-1) siRNA against bladder cancer in mice. PLK-1 regulates cell division and its expression is correlated with a variety of human tumors. The authors put the siRNA into vesicles that can enter cells via their membranes to suppress the expression of PLK-1 in bladder cancer cells in a time and dose dependent fashion.
Intravesical administration of the PLK-1 inhibitor reduced cell proliferation and killed cancer cells. To validate the efficacy of the treatment in vivo, mice were implanted with bladder cancer cells, and PLK-1 siRNA prevented the growth of bladder cancer in this mouse model.
This interesting study shows that therapy to inhibit expression of PLK-1 could be an important strategy for the management of bladder cancer.
TITLE: Intravesical administration of small interfering RNA targeting PLK-1 successfully prevents the growth of bladder cancer
AUTHOR CONTACT: Takeshi Yuasa
Kyoto University Hospital, Kyoto, Japan
Phone: 81-75-751-3630; Fax: 81-75-751-3631; E-mail: pochi1217@aol.com
View the PDF of this article at: https://www.the-jci.org/press/23043.pdf
From 5:00PM USA EST Thursday March 10, 2005 a PDF of this article will be available at: http://www.jci.org/papbyrecent.shtml
Journal of Clinical Investigation