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Development of a HepaRG human hepatocyte model with enhanced CYP2D6 activity

02.25.26 | Toho University

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Establishment and characterization of FLAG-CYP2D6 and CYP2D6-iGFP undifferentiated HepaRG cells
(A) Transgene structures for the expression of the exogenous FLAG-tagged CYP2D6 protein or the IRES-based co-expression of the human hepatic CYP2D6 and GFP proteins. (B) Phase contrast images of the FLAG-CYP2D6 HepaRG cell line. Scale bar, 200 µm. (C) Representative phase contrast and fluorescence images of CYP2D6-iGFP HepaRG cells. Scale bar, 200 µm. (D) Representative immunofluorescence images showing the subcellular localization of CYP2D6 in #3 FLAG-CYP2D6 and #1 CYP2D6-iGFP HepaRG cells. Sca Credit: Dr. Shinpei Yamaguchi

A research team led by Associate Professor Shinpei Yamaguchi and the late Professor Masako Tada of the Faculty of Science, together with Professor Yojiro Anzai and Lecturer Yohei Iizaka of the Faculty of Pharmaceutical Sciences at Toho University, have developed a new HepaRG cell line with markedly enhanced activity of CYP2D6, a major drug-metabolizing enzyme. This engineered human hepatocyte model enables more human-relevant evaluation of drug-induced hepatotoxicity mediated by CYP2D6. The study was published in the international journal PLOS ONE on December 29, 2025.

Key Points

PLOS One

10.1371/journal.pone.0339559

Experimental study

Cells

Development of a CYP2D6-enhanced HepaRG cell model with improved CYP2D6 metabolic capacity

29-Dec-2025

The authors have declared that no competing interests exist.

Additional Media

Dr. Shinpei Yamaguchi
Dr. Shinpei Yamaguchi, Associate Professor of the Faculty of Science at Toho University Credit: Dr. Shinpei Yamaguchi

Keywords

Hepatocytes

Article Information

Journal
PLOS One
DOI
10.1371/journal.pone.0339559
Method of Research
Experimental study
Subject of Research
Cells
Article Publication Date
2025-12-29
Article Title
Development of a CYP2D6-enhanced HepaRG cell model with improved CYP2D6 metabolic capacity
COI Statement
The authors have declared that no competing interests exist.

Contact Information

Akira Muto
Toho University
akira.muto@med.toho-u.ac.jp

Source

Original Source
https://global.toho-u.ac.jp/research/pr-1573/

How to Cite This Article

APA:
Toho University. (2026, February 25). Development of a HepaRG human hepatocyte model with enhanced CYP2D6 activity. Brightsurf News. https://www.brightsurf.com/news/1GRMYJ58/development-of-a-heparg-human-hepatocyte-model-with-enhanced-cyp2d6-activity.html
MLA:
"Development of a HepaRG human hepatocyte model with enhanced CYP2D6 activity." Brightsurf News, Feb. 25 2026, https://www.brightsurf.com/news/1GRMYJ58/development-of-a-heparg-human-hepatocyte-model-with-enhanced-cyp2d6-activity.html.