Scientists from the Institute of Vertebrate Paleontology and Paleoanthropology (IVPP) of the Chinese Academy of Sciences have uncovered new information suggesting a potential connection between Homo erectus and modern humans, while also developing new, less invasive paleoproteomics methods of fossil research.
Homo erectus , or H. erectus , was the first species within the genus Homo to leave Africa, occupying a key position in human evolutionary history. However, due to the lack of molecular evidence from H. erectus , their genetic characteristics, population diversity, and especially their potential connections to modern humans remain unresolved. As a result, the role of H. erectus represents a major mystery and a focal point of debate in human evolution.
Molecular research on H. erectus remains has been limited because ancient human fossils are irreplaceable and a precious cultural heritage. For this reason, traditional destructive sampling methods are unacceptable and have long constrained the progress of relevant molecular research.
Now, however, the research team, led by FU Qiaomei from IVPP in collaboration with multiple institutions, has overcome this bottleneck by employing a micro-destructive sampling approach based on acid etching to recover molecular information from six Homo erectus teeth without damaging their morphology.
The team’s findings were published online in Nature on May 13.
The article was also accompanied by a concurrent commentary in Nature , which highlighted the role of enamel proteins from these six H. erectus teeth from China in providing “new insights into how ancient genetic material was eventually introduced into modern human populations.”
The researchers identified two mutations from the fossil teeth, dating back to at least 400,000 years ago, from three different sites—Zhoukoudian (Peking Man), Hexian, and Sunjiadong. The mutations suggest genetic links between East Asian H. erectus and Denisovans, which themselves are linked to modern humans.
The first is the previously unknown AMBN-A253G mutation, which was identified as a potential molecular marker associated with these H. erectus populations. It provides the first evidence that H. erectus specimens from these three sites belonged to the same evolutionary population.
The second is the AMBN-M273V variant, previously thought to be specific to Denisovans. However, this study reveals that this variant is not unique to Denisovans but is shared by these H. erectus populations.
According to the researchers, the second variant may have entered the Denisovan lineage through admixture and was subsequently passed to some modern human populations (in Southeast Asia and Oceania) via Denisovan introgression. This provides the first insights into a possible connection between East Asian H. erectus (such as those from Zhoukoudian) and Denisovans, as well as the potential deep genetic links to some present-day modern humans.
Additionally, the study establishes a suite of new experimental and computational methodologies, including a sex determination method for ancient hominins based on the male-specific enamel protein AMELY, a cross-validation approach using tandem mass spectrometry and multiple data analysis pipelines, and DNA analysis methods linked to specific amino acid variants. Together, these tools provide a new framework for systematic paleoproteomics research.
Nature
Enamel proteins from six Homo erectus specimens across China
13-May-2026