Proteomics has become an essential area in medical research, propelled by the ability to quantitatively assess proteins on a large scale using high-throughput technologies. These technologies have unveiled new insights, such as the unexpected involvement of GAPDH in various cancers. While these findings have opened doors for clinical applications, they remain relatively limited, prompting the need to summarize recent advancements and explore future prospects.
Proteomic Technologies
The review elaborates on four key high-throughput proteomic techniques: mass spectrometry (MS), protein pathway array (PPA), next-generation tissue microarrays (ngTMA), and multiplex bead- or aptamer-based assays like Luminex® and Simoa®. Each of these techniques has unique strengths and limitations that dictate their appropriate usage in different clinical contexts. MS, for example, is crucial for analyzing protein isoforms and post-translational modifications, while PPA and ngTMA are better suited for large-scale antibody-based molecular analyses.
Advances in Clinical Applications
High-throughput proteomics has been instrumental in various fields, particularly cancer research. The review delves into its application across several types of cancers:
Challenges
Several challenges hinder the broader clinical application of high-throughput proteomics:
Advantages
Despite these challenges, high-throughput proteomics offers several advantages:
Future Directions
The review outlines several promising areas for future research in high-throughput proteomics:
Conclusions
The clinical application of high-throughput proteomics is set to grow significantly, driven by technological innovations and the increasing integration of proteomics with other omic data types. Overcoming current challenges will be crucial in realizing the full potential of proteomics in transforming translational research, clinical practice, and public health.
Full text:
https://www.xiahepublishing.com/2472-0712/ERHM-2024-00006
The study was recently published in the Exploratory Research and Hypothesis in Medicine .
Exploratory Research and Hypothesis in Medicine (ERHM) publishes original exploratory research articles and state-of-the-art reviews that focus on novel findings and the most recent scientific advances that support new hypotheses in medicine. The journal accepts a wide range of topics, including innovative diagnostic and therapeutic modalities as well as insightful theories related to the practice of medicine. The exploratory research published in ERHM does not necessarily need to be comprehensive and conclusive, but the study design must be solid, the methodologies must be reliable, the results must be true, and the hypothesis must be rational and justifiable with evidence.
Exploratory Research and Hypothesis in Medicine
Advances in the Clinical Application of High-throughput Proteomics
3-Jul-2024