Articles tagged with Gene Editing
Scientists at the University of Texas at Austin have redesigned a key component of the widely used CRISPR-based gene-editing tool Cas9 to be thousands of times less likely to target the wrong stretch of DNA. The new version, called SuperFi-Cas9, is as efficient as the original but reduces off-target interactions, making it potentially ...
Xue Sherry Gao has won a prestigious CAREER Award to create versatile toolkits for controlling gene expression. Her research aims to develop broadly applicable platforms for gene regulation, with a focus on precision dosing and safety features.
Researchers successfully edited the genomes of black-legged ticks using CRISPR-Cas9, overcoming technical challenges and advancing tick genetic research. The study's findings have significant implications for understanding tick-pathogen-host interactions and developing new approaches to tick-borne disease control.
Researchers have developed two new gene editing methods for ticks, allowing scientists to alter parts of the tick genome involved in harboring and transmitting pathogens. The methods use CRISPR/Cas9 technology and could lead to the development of new control methods for diseases like Lyme disease.
Researchers at Gladstone Institutes and UC San Francisco have developed a CRISPR activation method that allows them to activate genes in human immune cells, revealing key regulators of cytokine production. This breakthrough accelerates immunotherapy research and may lead to more powerful cancer treatments.
Gladstone Institutes researchers have pioneered a new method to edit genes in human cells using retrons, which can produce abundant copies of template DNA from inside cells. The optimized system has shown improved efficiency and precision compared to current approaches.
A new report by The Hastings Center suggests that releasing gene-edited species into the wild demands deep public engagement. The report proposes a path forward for inclusive, values-based decision-making through public deliberation.
Scientists at Oak Ridge National Laboratory developed a self-detect solution to monitor CRISPR gene editing tools in organisms. The system uses a biosensor guide RNA and reporter protein to trigger the technology's reveal itself, enabling real-time detection of CRISPR activity.
Researchers have developed a CRISPR/Cas9 gene editing system to enhance the effectiveness of sonodynamic therapy, allowing tumors to be effectively shrunk in a mouse model of liver cancer. The technology reduces antioxidant defense systems, increasing cancer cell death from the treatment.
Researchers have expanded the number of naturally occurring CRISPR-Cas systems, giving a wealth of potential new tools for large-scale gene editing. The discovery could lead to treating complex diseases associated with multiple genes.
Researchers from the University of Manchester have discovered a new way to manipulate key assembly line enzymes in bacteria using CRISPR-cas9 gene editing. This approach could lead to the production of improved antibiotics with potentially improved properties, addressing the growing threat of antimicrobial resistance.
Researchers at Karolinska Institutet found that CRISPR gene-editing causes DNA damage, activating the p53 protein, which can lead to an accumulation of mutated cancer cells. The study identified a network of linked genes with similar mutations and suggests transient inhibition of p53 as a potential strategy to prevent their enrichment.
Researchers at Princeton University developed a novel method called Repair-seq to understand genome editing tools, revealing complex mechanisms of DNA repair. This work improves the CRISPR gene-editing method by identifying new pathways and optimizing systems.
Researchers identify key neurons and signaling pathway regulating female activity before ovulation. Discoveries could lead to new treatments for menopause that sidestep estrogen and reactivates the circuit with CRISPRa technology.
Researchers at UMD are developing CRISPR-Combo systems to improve genome editing and crop regeneration. The technology aims to reduce the time and cost of breeding new crop varieties with enhanced nutritional and agronomic traits.
A compact CasMINI CRISPR system has been engineered for efficient gene regulation and editing in mammalian cells. The system, derived from the naturally occurring archaeal protein Cas12f, is highly specific and efficient, making it suitable for various therapeutic applications.
Researchers at Temple University's Lewis Katz School of Medicine have been awarded a prestigious grant from the National Institutes of Health to develop novel treatments for individuals living with HIV using CRISPR technology. The $4.8M grant will fund four major research laboratories working towards eliminating HIV DNA from infected c...
Isaac Hilton is using non-integrating episomal DNA viruses to create a new platform technology for cell and gene therapies. He aims to hijack these viruses to safely program medicinal functions in human cells.
Researchers have developed a new way to deliver molecular therapies to cells using a programmable system called SEND, which harnesses natural proteins in the body to encapsulate and deliver different RNA cargoes. This could lead to safer and more targeted delivery of gene editing and other molecular therapeutics.
Researchers have mapped the structure of CRISPR-Cas12j3 from bacteriophages, a discovery that reveals how it works and solves packaging problems for genome editing. The new system has vast potential for precise genome editing with improved efficiencies and alternative targeting mechanisms.
Yiping Qi's team will test new delivery technologies for CRISPR-Cas12a to develop a pipeline for genome editing in carrots. They aim to create more nutritious and hypoallergenic carrot varieties with improved nutritional value and reduced allergenicity.
A new genetic engineering platform has been established in methylotrophic yeast Pichia pastoris, enhancing homologous recombination rates and genome editing efficiency. This breakthrough can enable the stable loading of over 100 exogenous genes and precise regulating of gene expression.
The new CRISPR 3.0 system allows for simultaneous activation of up to seven genes at once, demonstrating high accuracy and efficiency. This multiplexed gene activation system has significant potential for crop breeding and enhancement, enabling the discovery and translational science in plants.
Researchers have successfully used CRISPR-Cas9 gene editing technology to manipulate glucosinolate levels in broccoli, reducing their accumulation in the leaves and florets. This study highlights the potential of gene editing to improve crop health and adaptability in challenging environments.
Researchers have developed a new method to select efficient gRNA molecules for CRISPR-Cas9 gene editing, achieving high efficiency and precision. The algorithm uses deep learning and large datasets to predict the efficiency of gRNAs, promising improved outcomes in genetic disorders and biotechnology applications.
Researchers at UT Southwestern Medical Center have successfully employed a new CRISPR-Cas9-based gene therapy approach to treat mice with Duchenne muscular dystrophy, restoring nearly full production of the dystrophin protein. The approach could lead to a treatment for DMD and inform the treatment of other inherited diseases.
An international research team proposes combining organic farming with gene editing to achieve greater sustainability in agriculture. The study suggests that this approach could lead to more robust plants with high yields, improved nutrition, and reduced environmental impact.
A peptide nucleic acid-based gene editing technique has been identified as a crucial part of the gene editing toolkit, offering an alternative to CRISPR-Cas systems. The technique, developed by Carnegie Mellon's Danith Ly and Yale University's Peter Glazer, allows for in vivo gene editing using nanoparticles.
Scientists report that prime editing successfully shuts down a gene involved in smooth muscle cell differentiation, correcting genetic problems and disease models. Prime editing is less complicated and more precise than traditional CRISPR, with fewer components and no collateral damage.
Scientists at MLU and IPB improve CRISPR/Cas9 to simultaneously knockout multiple genes in plants, overcoming previous limitations and enabling complex trait investigations. This approach allows for the study of gene interactions and redundancies, paving the way for more efficient research methods.
Scientists at the University of Queensland are using gene editing technologies to develop crops that can thrive in extreme and variable climate conditions. By integrating CRISPR-Cas9 genome editing into modern breeding programs, researchers aim to increase crop resilience and nutritional quality, ensuring global food security.
A new tool, DECODR, scans for potential errors in CRISPR gene repair, revealing risks to patients. The affordable app uses open-source software and can detect a wide range of DNA mutations at a fraction of the cost.
New gene editing strategies, including CRISPR technologies, are being investigated for treating inherited retinal diseases. Researchers aim to identify the most viable therapeutic approaches using human retinal tissue and organoids.
A newly engineered CRISPR variant called SpRY enables targeted mutation of nearly any genomic sequence in plants, expanding genome editing capabilities and opening up new possibilities for crop improvement. This breakthrough discovery is a significant advancement in the field of plant genome editing.
New gene editing strategies, including CRISPR technologies, are being investigated for treating inherited retinal diseases. The article highlights the most viable therapeutic approaches and discusses safety concerns and challenges in extending the capabilities of CRISPR-Cas9.
Researchers have developed a method to detect Cas9 protein, a key component of CRISPR/Cas gene editing technology, in human plasma and mouse models. This breakthrough aims to identify athletes who may be using gene doping to gain an unfair advantage.
A promising fix to CRISPR-Cas9's problem with unwanted genetic changes has been found using a method that turns off gene-editing until it reaches key cell cycle phases where more accurate repairs are likely to happen.
A new CRISPR/Cas9 variant, miCas9, has been developed to improve the efficiency of gene editing while reducing unintended insertions or deletions. This advancement holds promise for advancing gene editing in genetic research and potentially treating diseases.
Scientists at ChristianaCare's Gene Editing Institute are developing a novel gene therapy for inherited blood disorders like sickle cell disease using CRISPR technology. The team aims to identify genetic variations that affect treatment efficacy and create a tailored approach for personalized medicine.
Researchers at North Carolina State University recommend a coalition, CLEAR-GOV, to provide basic information about gene-edited crops for greater transparency. The proposed model would include details on plant species, trait modifications, and downstream uses.
Researchers have successfully applied CRISPR gene editing to influence the levels of beta-glucan in barley grain, with implications for brewing and distilling industries. The study provides insight into key genes responsible for barley grain composition, enabling plant breeders to accelerate breeding and develop new crop varieties.
A new study improves CRISPR gene editing by mutating the Cas9 enzyme to reduce off-target hits. The mutation increases fidelity up to 93-fold, making it a potentially safer strategy for gene therapy.
A new precision gene editor for mitochondrial DNA has been developed, allowing scientists to make targeted changes without the need for CRISPR technology. This breakthrough could enable researchers to study rare diseases and basic mitochondrial biology in animals.
A new machine learning model, BE-Hive, accurately predicts the outcomes of using different base editors to correct genetic mutations. The model discovered new properties and capabilities of base editors, allowing researchers to design novel tools with improved efficiency.
Researchers have developed a fast CRISPR system that reduces cleavage time from hours to seconds, allowing for high-resolution DNA repair studies and single allele-level genetic editing. The technique uses light-sensitive nucleotides to control Cas9's action, enabling precise control over the editing process.
Scientists at ChristianaCare's Gene Editing Institute have developed a new CRISPR advance that can safely target and disable the NRF2 gene linked to a bleak prognosis in lung cancer tumors. This approach aims to improve the efficacy of conventional chemotherapy and radiation treatments while minimizing harm to normal cells.
Researchers have achieved a groundbreaking milestone in treating inherited blindness by using CRISPR gene editing within the human body. The BRILLIANCE clinical trial aims to repair mutations causing Leber congenital amaurosis type 10, a rare form of blindness. This permanent approach avoids passing on genetic changes to offspring.
A hybrid approach combining gene therapy and gene editing successfully treated an experimental model of a rare genetic disease, significantly enhancing survival. The findings could offer hope for children and adults with various inborn errors of metabolism.
A Japanese survey reveals differing perceptions of gene-edited crops among experts and the general public. Experts see benefits, while the public perceives risks despite acknowledging value. The findings shed light on the deficit model's boundaries in science communication.
Researchers at Columbia University Irving Medical Center have developed a new gene editing tool called INTEGRATE, which uses cryo-electron microscopy to capture high-resolution images of the complex in action. The tool appears to work by targeting DNA for accurate insertion of genetic payloads without introducing DNA breaks.
Researchers have developed new methods that leverage growth regulators and gene editing reagents to trigger seedlings with edited genes. These approaches significantly reduce the time needed for gene-edited plant production from months to weeks, making it easier for small labs and companies to utilize.
Researchers at Harvard Medical School have successfully used CRISPR-Cas9 gene editing to disrupt both latent and active herpes virus in human cells. The findings offer a model system for using gene editing in a localized way to disrupt active replication, but the challenge of delivering gene-editing therapy to neurons remains unsolved.
A new study reveals a new tool that can analyze CRISPR edits in just 48 hours, identifying multiple outcomes of the process. The tool detects subtle mutations to DNA near the site of repair, which may have no consequence for patients but are essential to gauge patient risks.
A recent study published in Frontiers in Genetics confirms that CRISPR-Cas9 gene editing technology is highly accurate and minimizes off-target effects. The research, conducted on zebrafish, adds to a growing body of work suggesting that unintended mutations from gene editing are extremely rare.
A team led by biomedical engineer Kam Leong has received a $3.2 million NIH grant to develop efficient, noninvasive gene editing methods for the brain. The goal is to overcome current inefficiencies in delivering CRISPR-based gene editing elements to the brain and treat devastating diseases like Alzheimer's.
Researchers at the University of Iowa have made significant breakthroughs in delivering gene-editing proteins to airway cells without causing harm. The new peptide-based platform shows promise in treating diseases like cystic fibrosis, COPD, and asthma by repairing or modifying disease-causing mutations.
Scientists have quantified the rate of Cas9-caused off-target mutagenesis in mice through whole-genome sequencing, revealing that guide RNA design can significantly reduce unwanted mutations. The study highlights the need for improved precision in gene editing, particularly in therapeutic applications.
The Carnegie Mellon and Yale research team will scale up production of PNAs, improve DNA binding properties, and develop new nanoparticle formulations for enhanced in vivo editing. The goal is to move the technique closer to clinical therapeutic applications.
Researchers have developed a new CRISPR-Cas9 variant that reduces unintended changes in DNA, increasing precision in gene therapy. The SaCas9-HF variant shows high on-target efficiency and nearly undetectable off-target activity, offering a promising alternative for precise genome editing.
Scientists Zach Lippman and Yuval Eshed review past agricultural revolutions, highlighting key genetic mutations and modifications. They propose using CRISPR gene editing to introduce new variations in core hormonal systems, potentially boosting crop productivity and adaptability.