A more powerful way to develop therapeutics?
A new approach to discover key proteins necessary for biologics has been developed by Philip M. Kim at the University of Toronto, combining high-tech computer simulation and laboratory experiments.
Protein Analysis
Articles tagged with Protein Analysis
Smoking may have negative effects on sperm quality
A recent study published in BJU International found that smoking can cause DNA damage in sperm, leading to decreased fertility. The research included 20 non-smokers and 20 smokers and analyzed protein alterations in their sperm. Sperm with altered DNA may lead to health problems in offspring.
Protein-based risk score may help predict CV events among patients with heart disease
A protein-based risk score was developed and validated for predicting cardiovascular outcomes in patients with coronary heart disease. The score performed better than the Framingham secondary event risk model but provided modest discriminative accuracy.
Wilke Cohen Lyme Disease Project gets $3 million from the Steven & Alexandra Cohen Foundation
The Wilke Cohen Lyme Disease Project has received a $3 million pledge from the Steven & Alexandra Cohen Foundation to support its research into potential biomarkers for Lyme disease. The project aims to develop more effective diagnostics and treatments, building on initial discoveries made by ISB researchers.
Luminous proteins offer new method to discover viral infections
Researchers at Umeá University have developed a new method to directly follow viral infections in living organisms, making infected cells produce fluorescent proteins. This method enables the manipulation of cells to produce specific proteins, allowing for detailed study of immune system responses.
The new SLENDR technique: Protein labeling in the developing brain by genome editing
Researchers have developed a new method called SLENDR that allows precise labeling of proteins in brain cells using CRISPR/Cas9. This enables scientists to study brain development and function with unprecedented accuracy, revealing previously undescribed behaviors of protein kinase C.
DNA barcodes gone wild
A team of researchers has developed a new technology that can stitch together DNA barcodes inside cells, allowing scientists to search amongst millions of protein pairs for protein interactions. This breakthrough increases the rate of discovery without increasing costs.
New tool enables scientists to interpret 'dark matter' DNA
Scientists at the Gladstone Institutes have invented a new way to read and interpret the human genome, using machine learning technology to predict gene-enhancer interactions. The TargetFinder tool accurately predicts complex three-dimensional interactions up to 85% of the time, opening the door to treating genetic diseases.
Biosensors on demand
Scientists have developed a method to engineer custom biosensor proteins that can precisely sense specific molecules, expanding the variety of biosensor designs. The approach combines computational protein design, in vitro synthesis, and in vivo testing to identify tailored biosensors.
Pursuing Alzheimer's dsease from the periphery
Researchers aim to improve measurement methods for amyloid-beta protein production outside the brain, a key area of study for Alzheimer's disease. They plan to develop innovative quantitative proteomics methods to analyze peripheral tissue changes and their relation to brain changes.
UTA physicists devise gene therapy platform for macular degeneration patients
Researchers at UTA have developed a new platform that uses ultrafast near-infrared lasers to deliver gene therapy to damaged areas of the retina, enabling vision restoration in patients with macular degeneration. The laser-based method has been shown to be more effective than traditional chemical gene delivery systems.
New massive dataset of bacterial proteins
Scientists from Switzerland and the Netherlands have identified over 2,300 bacterial proteins in 22 different growth conditions, representing half of the bacterial genes. The dataset provides insight into protein function, expression levels, and post-translational adaptations.
Researchers find universality in protein locality
A team of researchers mapped a universal dynamic that explains protein production and distribution, with implications for understanding phenotypic variability. The findings suggest a fundamental role for cellular feedback mechanisms in regulating protein levels.
Protein conjugation method offers new possibilities for biomaterials
Researchers have demonstrated a novel method to create protein polymers that can display new functionalities, including temperature responsiveness. By changing the decoration points on the protein, they found that the functional properties of the polymer were influenced.
From protein design to self-driving cars: UW team wins AI prize for optimization approach
The UW optimization algorithm, RDIS, breaks down complex problems into smaller chunks, solving them exponentially faster. In protein design and self-driving car applications, RDIS performs significantly better than existing methods, accurately mapping images into realistic spaces.
The clock is ticking: New method reveals exact time of death after 10 days
Researchers at the University of Salzburg developed a new method to estimate time since death in humans, even up to 240 hours after death, by analyzing muscle proteins and enzymes in pigs. Initial results are promising for human samples, offering a simple and efficient approach with advantages over current methods.
EMBL scientists solve decades-old cell biology puzzle
Researchers at EMBL Heidelberg solved a decades-old cell biology puzzle by clarifying the behavior of clathrin proteins, crucial for endocytosis. The team used new imaging techniques to demonstrate that the surface area of the clathrin coat remains constant during endocytosis, only changing its curvature as it draws the cell membrane i...
TGen and Phoenix Children's Hospital research new ways to store, transport blood samples
Researchers are working on a project to stabilize high-quality blood plasma proteins using special filter papers. The goal is to make it possible for patients to provide their own blood samples from home, reducing protein degradation and improving existing protocols with a simple and cost-effective method.
New, robust and inexpensive technique for protein analysis in tissues
A new technique for detecting proteins in tissues has been developed at Uppsala University, enabling robust and inexpensive analysis. The method uses antibodies linked to DNA strands and emits light when attached, making it suitable for point-of-care devices and clinical applications.
Danish discovery accelerates targeted cancer treatment
Researchers have discovered how to map more than one protein at a time when DNA is damaged, enabling the development of better and gentler cancer treatments. This new technique allows for a clearer picture of the reparatory process, facilitating the discovery of specific proteins involved in repairing damaged DNA.
Cells amplify messenger RNA levels to set protein levels
A new statistical analysis of mRNA and protein levels in budding yeast found that mRNA levels account for 85% of differences in protein levels, amplifying the relationship between the two. This discovery contradicts previous studies suggesting that post-transcriptional regulation overrides mRNA levels.
Proteomics identifies DNA repair toolbox
Researchers at Max Planck Institute of Biochemistry have analyzed the protein composition of the DNA replication machinery in response to damaged DNA. They found that over 90 proteins are recruited to aid in repair, including many known factors as well as new proteins with unknown functions.
New protein booster may lead to better DNA vaccines and gene therapy
Scientists have discovered a new way to manipulate how cells function, improving the efficiency of DNA vaccines and gene therapy. The new method boosts protein production by up to 20 times, potentially leading to stronger immune responses and reduced side effects.
Cell imaging gets colorful
Researchers have developed a new method for detecting and imaging protein-protein interactions in live cells using color changes, enabling immediate visualization of biochemical events. The FPX technique converts biochemical processes into dramatic green to red color changes.
First major analysis of Human Protein Atlas is published in Science
The study analyzed approximately 20,000 protein coding genes in humans, revealing almost half are expressed ubiquitously across tissues. It also showed that 70% of approved pharmaceutical drugs target either secreted or membrane-bound proteins.
How does a machine smell? Better than it did
Researchers at the University of Manchester and University of Bari have created a biosensor that can differentiate between mirror image smells. The system uses odorant binding proteins to detect unique chemical changes, approaching human nose detection limits.
Researchers develop novel method to prevent, cure rotavirus infection
A Georgia State University research team has developed a novel method to prevent and cure rotavirus infection by activating the innate immune system with the bacterial protein flagellin. This approach triggered an immune response that prevented the virus from entering cells and removed existing infections.
Sopping up proteins with thermosponges
The study demonstrates that thermosponge nanoparticles can effectively deliver a variety of proteins while preserving their biological activity. The new platform is designed to eliminate the need for harsh solvents and shows promise for delivering protein-based drugs for human therapeutics.
Frozen meal eaters have better intakes of key nutrients for fewer calories than QSR eaters
Consumers of frozen meals had higher daily intakes of dietary fiber, potassium, calcium and protein, while QSR consumers had lower intakes. Frozen meal eaters also consumed 253 fewer calories and 2.6 grams less saturated fat per day.
New knowledge of cannabis paves the way for drug development
Researchers at the University of Copenhagen have developed a new screening method that reduces precious sample consumption by a billion times, enabling faster and cheaper drug development. The method uses nanotechnology to study cell membrane proteins binding drugs like cannabis and adrenaline.
Genetics and lifestyle have a strong impact on biomarkers for inflammation and cancer
A large-scale study by Uppsala University researchers reveals that genetics and lifestyle factors play a crucial role in protein levels, enabling the use of more effective biomarkers. The study analyzed 92 protein biomarkers in 1,000 healthy individuals, finding that hereditary factors contribute to over 75% of proteins.
Carnegie Mellon chemists create nanofibers using unprecedented new method
Researchers developed novel protein/polymer nanostructures, reminiscent of living cell fibers, for material fabrication. The modified GFP molecules formed long fibers that disassembled with sound waves and reassembled within days.
New method provides researchers with efficient tool for tagging proteins
Aarhus University researchers have created an easier method to tag proteins with DNA, enhancing diagnostic techniques, nanotechnology, and disease treatment. The new method allows for controlled conjugation of macromolecules, making it possible to attach chemotherapeutics to antibodies while preserving their recognition element.
Neuroprotective effects of low concentration of lithium
The study found that low concentrations of lithium carbonate had a protective effect on SH-SY5Y cells, with reduced expression levels of pyruvate kinase 2 and calmodulin 3. Additionally, western blot analysis revealed increased expression of stress proteins GRP94 and HSP27.
Food allergies: A new, simple method to track down allergens
Researchers at EPFL have created a new method to track down allergens in foods, which can help develop customized therapies and provide higher accuracy than conventional allergy-testing methods. The method uses immunoaffinity capillary electrophoresis and mass spectrometry to identify specific proteins causing allergic reactions.
Cellular team players
Researchers at Technical University of Munich used FRET methodology to observe interaction between Hsp90, P23 and ATP. They found that P23 strengthens ATP bonding, increasing energy production. This breakthrough reveals the importance of cooperation in cellular energy generation.
Studies provide important new information on genetic risk of sudden cardiac death
Researchers from MGH have uncovered new information about genes that may increase the risk of serious cardiac arrhythmias. The studies identified a greater role for calcium signaling in regulating cardiac function and pinpointed specific genes involved in the biology of cardiac repolarization.
Researchers discover 'Trojan Horse' method of penetrating cellular walls without harm
Researchers have found a way to deliver proteins directly into live human cells, bypassing the need to damage or kill the cell membrane. This breakthrough has the potential to revolutionize medical research and treatment of diseases, including cancer and regenerative medicine.
Watching HIV bud from cells
University of Utah researchers developed a way to observe HIV budding without interfering with the process, showing ALIX's involvement in the late stages of virus replication. The study used digital camera and microscope technology to make movies and photos of the budding process.
Following a protein's travel inside cells is key to improving patient monitoring, drug development
Researchers at Virginia Tech developed a novel method to detect the subcellular location of proteins, which will enable improved patient monitoring and drug development. The technique allows for rapid screening of large cell populations with high resolution, revealing heterogeneity among cells.
JCI online ahead of print table of contents for March 10, 2014
Scientists identified a broadly neutralizing HIV-1 antibody in a lupus patient, which could be used to target rapidly mutating viruses. Researchers also developed a workflow to evaluate protein turnover kinetics, revealing previously unknown alterations in disease development.
Designer proteins provide new information about the body's signal processes
Researchers at the University of Copenhagen have created 22 semi-synthetic designer proteins that can regulate specific biochemical tasks. The discovery provides unique molecular understanding of protein interactions, which could lead to more effective pharmaceuticals targeting stroke, pain, and depression.
Penn biologists establish new method for studying RNA's regulatory 'footprint'
Researchers have created a technique called PIP-seq to map all interactions between RNA and proteins. The study identifies potential disease-causing genetic mutations in regions of RNAs where RNA-binding proteins bind, providing new insights into neurological diseases like Parkinson's. The data is publicly available online.
Novel method could help bring cancer biomarkers to clinic
A new method developed by Fred Hutchinson Cancer Center researchers can measure hundreds of proteins in a single assay, paving the way for standardized quantification of the human proteome. This breakthrough could lead to more accurate cancer biomarkers and personalized treatments.
New testing strategy detects population-wide vitamin and mineral deficiencies
Researchers have developed a new testing strategy that can estimate levels of essential vitamins and minerals without directly testing each nutritional factor. This approach uses protein levels in the bloodstream as proxies for nutrient levels, enabling rapid detection of population-wide deficiencies and potential remediation efforts.
New protein knowledge offers hope for better cancer treatment
Researchers have developed an advanced method to identify proteins modified with ADP-ribosylation, shedding light on PARP inhibitor treatment for breast and ovarian cancers. The new analysis method enables optimized treatment with fewer side effects, offering improved cancer treatment options.
Chemists develop innovative nano-sensors for multiple proteins
Chemists at Johannes Gutenberg University Mainz developed a new method for parallel protein analysis, detecting hundreds or thousands of different proteins. The test strips bearing gold nano-particles can identify specific proteins in tiny samples, making it suitable for rapid disease diagnosis and environmental toxin detection.
Polymer coatings a key step toward oral delivery of protein-based drugs
Researchers at Brown University have developed a bioadhesive coating that significantly increases the intestinal uptake of polymer nanoparticles in rats. The coating enhances the distribution of particles to specific tissues around the body, suggesting potential for targeted oral drug delivery.
Researchers strike gold with nanotech vaccine
Researchers have developed a novel vaccination method using gold nanoparticles that can deliver specific proteins to the body's immune cells. The technique mimics viruses and stimulates an immune response, offering significant safety advantages.
Early exposure to bisphenol A might damage the enamel of teeth
Research suggests that early exposure to bisphenol A can damage the enamel of teeth in children, similar to a previously identified pathology known as MIH. The study found that low doses of BPA can cause mineral depletion and lead to hypomineralisation, making teeth more fragile and prone to cavities.
A trick to fold proteins more quickly
Researchers at SISSA have devised a trick to speed up the analysis of protein dynamics using computer simulations. By exploiting experimental data and mathematical rules, they reduce simulation times by an order of magnitude, allowing for faster research in this field.
Researchers receive high honor from American Society of Plant Biologists
Natasha Raikhel, a distinguished professor at UC Riverside, has been named recipient of the Adolph E. Gude, Jr. Award for outstanding service to plant biology. Susan Wessler, also from UC Riverside, is a fellow of the ASPB for her pioneering work on plant transposable elements and genomes.
New dietary analysis tool for athletes debuts
A new website application called Dietary Analysis Tool for Athletes (D.A.T.A.) has been validated as accurately recording dietary intake. The digital tool generates a report immediately after the recall, providing quick feedback for athletes and sports health professionals.
Genomic data are growing, but what do we really know?
A large-scale evaluation of computational protein function prediction reveals that algorithms combining disparate prediction clues provide more accurate predictions, turning genomic data into useful information. The study provides a comprehensive picture of how well current methods perform, with potential improvements identified.
DNA barcoding alone sufficient to detect fraudulent deer products
Scientists from Hong Kong have developed a DNA barcoding method that can detect fraudulent deer products, regardless of their physical state. The method confirms that DNA barcoding alone is sufficient to detect such substitution for deer in all tendon products, except for glue.
Nature Methods study: Using light to control cell clustering
A new study from Rensselaer Polytechnic Institute and UC Berkeley uses blue light to activate specific proteins into large clusters, controlling cell signaling. This technique has potential applications in understanding cellular function and optimizing energy production.
Using lysine estimates to detect heat damage in DDGS
Heat damage in DDGS compromises nutritional value, but researchers predict digestibility using lysine estimates. The study found that analyzing lysine or furosine concentrations improves prediction accuracy.
Researchers decipher the mecanism of membrane fission
Scientists discovered that dynamin polymerises, forming a helix around an artificial membrane tubule and compressing it until it breaks. The location of the fission is specific, appearing at the boundary between the helix and the membrane.
Cellular eavesdropping made easy
A new approach devised by EMBL scientists enables the distinction of proteins secreted by cells from those in their food, allowing measurement of secretion changes over time. This method has opened new avenues for drug and biomarker screening, as well as studying cell responses to drugs and 3D growth conditions.
New uses for old tools could boost biodiesel output
Researchers use near infrared reflectance spectroscopy to assess seed oil quality and quantity, potentially boosting biodiesel output by optimizing oilseed yields and reducing costs