Articles tagged with Crisprs
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
Scientists developed a new CRISPR screen technology to target RNA, enabling accurate and fast detection of specific RNA targets. The technology uses Cas13 enzymes to identify key genes involved in various diseases, including cancer and sickle-cell anemia.
Researchers used CRISPR-Cas system to effectively target and eliminate specific gut bacteria, including Clostridioides difficile, the pathogen that causes colitis. The study demonstrates the potential of this approach in preventing disease and promoting human gut health.
A new CRISPR-Cas12b system enables efficient plant genome engineering with gene editing, activation and repression capabilities. The system outperforms existing CRISPR tools, offering improved efficiency and versatility for plant breeding and disease resistance.
A new CRISPR gene drive system, TARE, has been developed that can delay resistance and spread to regional populations. By targeting a essential gene, the drive disables one copy while leaving another intact, allowing it to spread through a population over time.
Scientists from the University of Illinois at Urbana-Champaign developed a new CRISPR gene-editing methodology that inactivated a key gene responsible for ALS, slowing disease progression and improving muscle function. The treatment also increased overall survival in mice with aggressive forms of ALS.
A recent study published in Nature found that CRISPR anti-viral immunity is often a disadvantage to bacteria when infected by certain viruses. The research suggests that triggering the powerful defense systems can be risky for a bacterium, leading to significant implications for treatment design.
The discovery of CRISPR-Cas9 systems has revolutionized pharmaceutical research by allowing for industrial-scale gene editing and functional genomic screening. This enables the identification of new biological targets for precision medicines and the exploration of mechanisms of drug resistance and sensitivity ahead of clinical trials.
Scientists have successfully used CRISPR/Cas9 base-editing to cure cystic fibrosis in human stem cells, providing a promising new approach for treating genetic diseases. The technique, which repairs mutations without cutting DNA, shows great promise for the future treatment of various genetic disorders.
Researchers at Arizona State University have developed a new method called TREE to edit genes implicated in Alzheimer's disease, achieving 90% efficiency in human stem cells. The breakthrough uses base editors to make single DNA edits with high accuracy, paving the way for personalized medicine and disease modeling.
Researchers have developed DNA-binding editorial assistants to open up genes obscured by chromatin packaging, enabling CRISPR editing. This breakthrough enhances CRISPR efficiency and moves towards genetic-based assaults on diseases.
Researchers at the University of Illinois have developed a new technique to increase CRISPR-Cas9 efficiency, achieving up to five times higher rates of inserting genes into human cells. This breakthrough has significant implications for clinical gene-therapy applications and basic biological research.
Researchers have developed a new functional genomics system, MAGIC, which allows them to modulate the activity of multiple genes in concert. By combining individual gene edits with custom DNA sequences, scientists can explore synergistic effects and better understand complex traits.
Researchers at Columbia University Irving Medical Center have developed a new gene editing tool called INTEGRATE, which uses cryo-electron microscopy to capture high-resolution images of the complex in action. The tool appears to work by targeting DNA for accurate insertion of genetic payloads without introducing DNA breaks.
Phages construct an impenetrable compartment to protect their vulnerable DNA from CRISPR and restriction enzymes. This unique mechanism makes them virtually indestructible, with only two jumbo phages showing pan-CRISPR resistance.
Japanese researchers have developed a Class 1 CRISPR gene editing system that enables efficient DNA repairs in human cells with minimal off-target effects. The Cas3 protein-based approach achieves superior genome editing efficiency compared to traditional Class 2 systems, opening doors for new therapeutic applications.
A new study reveals a new tool that can analyze CRISPR edits in just 48 hours, identifying multiple outcomes of the process. The tool detects subtle mutations to DNA near the site of repair, which may have no consequence for patients but are essential to gauge patient risks.
Researchers at Case Western Reserve University have developed an electrochemical CRISPR platform that can detect specific viruses, such as HPV and parvo, in a single droplet of blood. This innovative device has the potential to provide accurate results in under an hour, compared to existing tests which take three to five days.
Researchers have developed a new CRISPR-Cas9 protein, SaCas9-HF, to increase the precision of genome editing. The new variant shows high accuracy in targeting human cells without compromising on-target efficiency.
A new CRISPR approach called prime editing has been developed by combining two key proteins and a new RNA to make targeted insertions, deletions, and single-letter changes in human cells. The system expands the scope of gene editing with up to 89% precision and potential correction of disease-causing genetic variations.
A new peer-reviewed journal discusses human genome editing's pros and cons, including concerns over non-Mendelian conditions and governance. The CRISPR Journal special issue explores various topics, from germline editing to gene therapy, with recommendations for democratic governance.
The CRISPR Journal publishes special issue on human genome editing ethics, exploring governance, moratoriums, and access. Experts argue for democratic governance and against imposing moratoriums, highlighting the need to regulate germline editing for safety and efficacy.
CRISPR-BEST addresses the challenge of genetic instability caused by double-stranded breaks in genome editing. The new tool creates mutations in actinomycetes without DNA breakage, enabling efficient targeting of genes for bioactive compound production.
A new CRISPR delivery system developed by Western University researchers enables targeted attacks on specific bacteria, including Staph A and E. coli. This breakthrough has the potential to create personalized antimicrobial agents and revolutionize the treatment of bacterial infections.
Researchers at UC Davis aim to deliver CRISPR genome editing machinery to gut cells to fix genes responsible for a rare form of familial cancer. They will use an engineered, non-infectious hepatitis E virus to orally deliver CRISPR into cells in the gastrointestinal tract of mice.
Researchers have developed a new CRISPR technology to accurately regulate and edit genomes in human cells, opening up nearly 90% of CRISPR-Cas systems. This approach has shown promise for biomedical research, gene therapies, and other applications.
The Crispr method enables researchers to monitor proteins' function live under natural conditions, eliminating the need for overproduction. This facilitates analysis of genes and gene products, allowing for more accurate results.
Researchers have successfully corrected the genetic mutation responsible for Duchenne muscular dystrophy using CRISPR gene editing in muscle stem cells. The edited cells regenerated and produced dystrophin, suggesting a potential method for lifelong correction of the disorder.
The National Institutes of Health has granted $2.23 million in funding to develop statistical and computational methods for genome-wide CRISPR/Cas9 screening. The goal is to improve functional gene identification, analyze non-coding elements, and study genetic interactions, with potential applications in cancer research.
Researchers created a deformable nanolipogel-based delivery system for CRISPR knockout of oncogene Lcn2, reducing tumor growth by 77% in human and mouse models. The delivery method shows promise as a precise therapeutic tool for treating triple-negative breast cancer.
A new CRISPR gene editing system, encapsulated in a nanolipogel, effectively targets and knocks out the Lcn2 oncogene, curbing tumor growth in mice, providing a potential genetic treatment for triple-negative breast cancer.
The CRISPR Journal has published new articles on iCas9, a tool that enables precise gene editing without DNA breakage. Researchers also developed BEAT, a computational program to quantify base editing outcomes. Additionally, the journal reported on identifying genetic vulnerabilities in cancer cells via CRISPR-Cas9.
Researchers at UCSF and NIH create a new CRISPR technique that allows them to systematically alter gene activity in human neurons, enabling the study of neurological diseases. They discovered that housekeeping genes behave differently in neurons and stem cells, suggesting that these differences may play important roles in disease.
RESCUE, a new CRISPR platform, allows for targeted RNA edits previously impossible, offering a critical gap in the toolbox for treating diverse genetic changes. The technology can modulate protein activity by targeting phosphorylation sites, providing a reversible alternative to DNA-level modifications.
The University of Maryland's Yiping Qi reviews the current state and future of CRISPR technology in crops, suggesting applications beyond traditional gene editing. He aims to enhance traditional breeding techniques with CRISPR to ensure global food and nutritional security.
The Taniguchi Lab at MPFI has developed a novel protocol combining laser microdissection with single-cell genotyping to accurately link observed phenotypes to underlying genetics. This approach enables the reliable determination of exact genetic causes, particularly for genes in the brain that have subtle effects.
Scientists have captured atomic-level images of active CRISPR enzyme Cas9, providing new structural information on its mechanism. The images show how the enzyme cuts DNA strands and reveals the importance of domain movement during reaction, which could lead to improved genome-editing tools.
Researchers have developed a novel CRISPR-based platform called SHERLOCK that enables the detection and quantification of plant genes. The platform is rapid, portable, and low-cost, with high multiplexing capability, making it an important tool for agriculture in detecting pathogens or pests and in plant breeding.
Researchers have developed a rapid CRISPR-Cas13 detection system for agricultural applications, enabling trait screening and pest surveillance. A new library-based assay predicts Cas9 specificity, addressing off-target effects in gene editing therapies.
A proof-of-principle study shows that gold nanoparticles loaded with CRISPR safely and effectively edited blood stem cells in lab models of HIV and inherited blood disorders. The researchers found that the Cas12a protein partner delivered precise genetic edits, which were maintained for eight weeks after injection.
Researchers have identified a single gene, Lsdia1, responsible for snail shell coiling in a species of freshwater snail. The study reveals that this gene controls left-right asymmetry from the earliest stages of development.
BioBits Health, a Northwestern University-led project, introduces CRISPR and antibiotic resistance to high school students. Students perform experiments using freeze-dried cell-free reactions, visualizing DNA editing and drug resistance, and exploring ethics.
A new bioinformatics tool analyzes CRISPR pooled screen data to identify candidate genes involved in diseases, outperforming existing methods. The web-based tool is quicker and more user-friendly, empowering non-bioinformaticians to analyze data.
Researchers at Arizona State University have developed a method to render the CRISPR-Cas9 gene editing tool 'immunsilent', allowing for reliable and stealthy gene repair. This breakthrough brings CRISPR closer to safe clinical application, addressing key safety concerns.
A team of scientists at the Gladstone Institutes has developed a reliable method to identify potential off-target effects in therapeutically relevant cell types. The DISCOVER-Seq technique uses DNA repair factors to pinpoint exact sites where CRISPR cuts occur, enabling more accurate genome editing.
A breakthrough CRISPR gene-editing tool allows for the simultaneous execution of multiple edits in DNA extracted from human cells. This technology, developed by the Gene Editing Institute and licensed to NovellusDx, can rapidly reproduce an individual patient's cancer tumor genetic features and identify driver mutations.
The CRISPR Journal publishes research on gene editing technologies, including base editors that enable precise base substitutions without DNA cleavage. A new method for multiplex site-directed mutagenesis also offers great promise for studying gene function.
Biomedical engineers at Duke University developed a method to improve CRISPR accuracy by adding a short tail to the guide RNA, creating a 'lock' that prevents off-target activity. The approach increases accuracy by an average of 50-fold across five different CRISPR systems.
Scientists at UC San Diego developed a new CRISPR-based 'allelic drive' to selectively swap genetic variants, allowing precise editing of specific traits in populations. The technology has potential applications in agricultural pests, disease-carrying insects, and conservation efforts.
A new CRISPR-Cas3 tool has been developed for long-range DNA editing in human cells, allowing scientists to target and delete large expanses of DNA. This technique harnesses a different type of CRISPR system than the widely used Cas9 tools, enabling precise control over DNA degradation.
Researchers are exploring RNA editing as a way to treat diseases without permanent genetic changes. This approach uses an enzyme called ADAR to make precise edits to RNA, which can be reversible and avoid the risks of CRISPR.
Researchers have developed a CRISPR-based graphene biosensor that enables digital detection of DNA without amplification, allowing for fast and accurate genetic mutation testing. The system uses CRISPR's genome-searching capability and graphene's sensitivity to detect target genes without amplification.
A new CRISPR-based device, CRISPR-Chip, can detect specific genetic mutations in a matter of minutes. The device uses graphene transistors to scan DNA samples and report results electronically, bypassing the need for polymerase chain reaction amplification.
Researchers have discovered how viruses evade detection by bacteria using a molecular decoy that tricks the CRISPR defense. This breakthrough expands scientists' understanding of viral strategies and raises possibilities for crafting anti-CRISPRs in the lab.
Researchers at UT Southwestern Medical Center discovered that adjusting CRISPR dosages can significantly improve dystrophin production in edited genes. The optimal ratio of components changed based on the DNA sequence being edited, paving the way for optimized gene therapies for other diseases.
Researchers at IBS have identified the mistake-rate of DNA editing tools using CRISPR and adenine base editors. The study reveals that ABE7.10, a widely used gene engineering tool, has an average of 60 off-target mistakes in the human genome.
A new CMP-fusion strategy called CRISPR-chrom enhances CRISPR-Cas9 genome editing efficiency, especially at previously difficult-to-target sites. The approach demonstrates a substantial increase in CRISPR-Cas9 activity with no notable increase in off-target effects.
The CRISPR Journal announces publication of its February 2019 issue, featuring studies on strain tracking, single-step genome editing, and chromatin modulating motifs. Researchers discuss advancements in CRISPR technology and its applications.
Researchers at Duke University found that a single CRISPR treatment can safely correct genetic disease for over a year, despite immune responses. The study suggests approaches to address potential challenges and potentially deliver the therapy to infants or restrict Cas9 expression.
Researchers at IDIBELL have developed Nested CRISPR, a cloning-free method for genome editing using long DNA fragments. The technique involves two steps: inserting a small portion of the fragment into the genome and then using it as a
Researchers have developed two molecular safeguards to prevent accidental spread of CRISPR gene drives in the lab. Synthetic target site drive and split drive strategies show similar performance to standard drives, making them suitable substitutes for early gene-drive research.