Articles tagged with Crisprs
Researchers at UC Berkeley have developed a new CRISPR editing technology that enables simultaneous editing of genes in multiple cell types and species within a microbial community. This approach, called community editing, has the potential to track edited microbes and understand their functions within complex ecosystems.
Researchers have expanded the number of naturally occurring CRISPR-Cas systems, giving a wealth of potential new tools for large-scale gene editing. The discovery could lead to treating complex diseases associated with multiple genes.
Researchers at Karolinska Institutet found that CRISPR gene-editing causes DNA damage, activating the p53 protein, which can lead to an accumulation of mutated cancer cells. The study identified a network of linked genes with similar mutations and suggests transient inhibition of p53 as a potential strategy to prevent their enrichment.
A recent study by Sanford Burnham Prebys and the National Cancer Institute has shown that CRISPR-Cas9 gene editing can favor cells with mutated forms of genes linked to cancer. This highlights the importance of monitoring patients undergoing CRISPR-based gene therapy for cancer-related mutations.
A new study published in Science Advances has shed light on the genetic basis of human appearance features by investigating the role of Hox genes. The researchers replaced the proboscipedia gene in a common laboratory fruit fly with its counterpart from a rarer Hawaiian cousin, revealing that Hox genes function as scaffolds for downstr...
Researchers used CRISPR gene-editing tools to show that a gene controlling bone growth in fish fins plays the same role in forming fingers and toes in four-legged creatures. The study suggests that the last common ancestor between ray- and lobe-finned fish already had the genetic toolkit to shape their appendages.
Researchers at UC San Diego have created a CRISPR-based rapid diagnostic technology that detects SARS-CoV-2, the coronavirus causing COVID-19. The new SENSR system is designed to simplify SARS-CoV-2 detection with a goal of eventual adaptation for in-home use.
A study by EPFL researchers reveals that CTCF sites within the HoxD cluster contribute to organizing genes into topologically associated domains, helping to organize developmental complexity. The dual function of CTCF binding sites varies depending on tissue type.
Researchers identify key neurons and signaling pathway regulating female activity before ovulation. Discoveries could lead to new treatments for menopause that sidestep estrogen and reactivates the circuit with CRISPRa technology.
A team of Harvard researchers created an integrated pipeline, STAMPScreen, to help genetic engineers identify target genes and perform screening studies. The protocol combines computational tools with lab experiments to quickly and efficiently test gene function in living cells.
Researchers at UC San Diego develop precision-guided sterile insect technique (pgSIT) to control mosquito populations. The system uses CRISPR to alter genes linked to male fertility and female flight in Aedes aegypti, reducing disease transmission.
Researchers at UMD are developing CRISPR-Combo systems to improve genome editing and crop regeneration. The technology aims to reduce the time and cost of breeding new crop varieties with enhanced nutritional and agronomic traits.
A compact CasMINI CRISPR system has been engineered for efficient gene regulation and editing in mammalian cells. The system, derived from the naturally occurring archaeal protein Cas12f, is highly specific and efficient, making it suitable for various therapeutic applications.
Stanford researchers have developed a mini CRISPR genome editing system that is smaller and more efficient than existing versions. The new system, called CasMINI, has been successfully tested in human cells and shows promise for treating various diseases, including eye disease, organ degeneration, and genetic diseases.
A CRISPR screening tool identified ZMYND8, an epigenetic regulatory protein, as a potential new therapeutic target for acute myeloid leukemia. Inhibiting ZMYND8 has been shown to leave cancer cells with smaller tumors and better survival in mouse models.
The new MIC-Drop technology enables rapid evaluation of hundreds of genes in a single experiment, improving large-scale genetic studies. Researchers can identify essential genes for healthy heart development and function.
Scientists have repurposed CRISPR to identify antibodies in patient blood samples, demonstrating a new class of medical diagnostics. The technique uses customizable proteins attached to Cas9, which assemble on a microchip to bind to specific DNA sequences, allowing for fast and accurate detection.
Researchers have mapped the structure of CRISPR-Cas12j3 from bacteriophages, a discovery that reveals how it works and solves packaging problems for genome editing. The new system has vast potential for precise genome editing with improved efficiencies and alternative targeting mechanisms.
Researchers at UC Berkeley created a rapid COVID-19 diagnostic test utilizing tandem CRISPR nucleases, which accelerates RNA detection and reduces sample handling. This innovation simplifies the assay process, making it faster and more efficient for SARS-CoV-2 virus detection.
Researchers at the University of Oregon used CRISPR-Cas9 gene editing to target a specific mutation causing Fuchs' corneal dystrophy, preserving endothelial cell density and function. The study lays the groundwork for future research on using this technique to treat genetic disorders in post-mitotic cells.
Researchers developed chemically modified guide RNAs for an RNA-targeting CRISPR system, significantly enhancing the ability to target and edit RNA in human cells. The optimized guides increased efficiencies of CRISPR activity by 2- to 5-fold and extended targeting activity from 48 hours to four days.
Yiping Qi's team will test new delivery technologies for CRISPR-Cas12a to develop a pipeline for genome editing in carrots. They aim to create more nutritious and hypoallergenic carrot varieties with improved nutritional value and reduced allergenicity.
The new CRISPR 3.0 system allows for simultaneous activation of up to seven genes at once, demonstrating high accuracy and efficiency. This multiplexed gene activation system has significant potential for crop breeding and enhancement, enabling the discovery and translational science in plants.
Researchers have successfully catalogued the effect of individual genes on human neuron function and survival, revealing unexpected results. The team's findings suggest that switching off certain genes can lead to an increase in oxidative stress, which may contribute to neurodegenerative diseases.
The study created a large-scale atlas of chromatin accessibility changes with the loss of individual enzymes, revealing how DNA is organized in cancer cells. Chromatin modifications are linked to diverse human traits and diseases, most notably cancer, where their loss results in global gene expression changes.
The use of CRISPR technology demands more robust public engagement to harness its benefits without crossing ethical lines. Effective communication that incorporates social science research can facilitate nuanced debates about the societal implications of genetic editing.
Scientists at Beam Therapeutics have created a redesigned base editor that successfully repairs the single-base mutation causing sickle-cell disease. The therapy targets an upstream regulatory pathway to express fetal hemoglobin, offering a potential solution for this genetic disorder.
Scientists have developed a novel CRISPR-based tool called CRISPRoff, which allows for the silencing of almost any gene in human cells without making DNA edits. This technology has significant therapeutic potential, particularly for rare genetic disorders that are caused by a single damaged copy of a gene.
Scientists report that prime editing successfully shuts down a gene involved in smooth muscle cell differentiation, correcting genetic problems and disease models. Prime editing is less complicated and more precise than traditional CRISPR, with fewer components and no collateral damage.
Researchers at UC San Diego developed a gene therapy that temporarily represses a gene involved in sensing pain, increasing pain tolerance and providing months of relief. The therapy could be used for various chronic pain conditions, including lower back pain and rare neuropathic disorders.
Researchers discovered that the WOX9 gene can induce more flowers to form in various plant species by changing its regulation, suggesting potential for improving crop yields. Genome editing allowed them to reveal the gene's additional functions without altering its protein product.
Researchers at Tufts University use lipid nanoparticles to deliver CRISPR machinery specifically to the liver, reducing blood cholesterol levels by up to 57% in mice. The treatment may provide a long-term solution for high cholesterol, which affects over 29 million Americans.
Stanford researchers developed a novel technique attaching nanobodies to CRISPR for targeted gene control. This combo enables precise on/off switching of specific genes, potentially correcting epigenetic defects without combining large effectors.
Researchers used DNA origami to analyze ultra-fast movements of CRISPR enzymes, enabling them to understand how they recognize target sequences. This technique will help optimize CRISPR for fewer off-target matches and improve gene editing processes.
Researchers have developed a new method, Cre-Controlled CRISPR, which combines the benefits of the Cre/lox system and CRISPR/Cas9 genetic scissors for conditional gene inactivation. This approach allows for faster and easier gene editing with reduced labor needed to flank genes with lox sequences.
Researchers have discovered a new gene-editing technique that allows for the programming of sequential cuts in genes, enabling researchers to better investigate time-sensitive processes like cancer development. The discovery was made by UIC's Bradley Merrill and colleagues using special molecules called 'proGuides',
Researchers at Cold Spring Harbor Laboratory have successfully applied CRISPR to increase corn kernel numbers by targeting promoter regions that regulate gene activity. This technique holds promise for increasing crop yields per acre and making agriculture more sustainable.
A new tool, DECODR, scans for potential errors in CRISPR gene repair, revealing risks to patients. The affordable app uses open-source software and can detect a wide range of DNA mutations at a fraction of the cost.
Researchers have discovered that TALEN is up to five times more efficient than CRISPR-Cas9 in targeting densely packed DNA regions, including those causing fragile X syndrome and sickle cell anemia. This breakthrough adds to the need for a broader selection of genome-editing tools to target all parts of the genome.
The CRISPR Journal has published a special issue on expanding the CRISPR toolbox, featuring new research tools and discoveries. The collection includes articles on novel Type V-A Cas12 enzymes, in silico tools for CRISPR-Cas system identification, and advances in guide design and selection.
A new CRISPR-based technique allows researchers to profile a cell's entire genome and identify the DNA sequences regulating specific genes. This enables simultaneous testing of thousands of experiments, paving the way for faster discovery of genetic networks and potential therapeutic targets.
Scientists at ChristianaCare's Gene Editing Institute are developing a novel gene therapy for inherited blood disorders like sickle cell disease using CRISPR technology. The team aims to identify genetic variations that affect treatment efficacy and create a tailored approach for personalized medicine.
Researchers have developed a safer, more targeted way to deliver CRISPR gene therapy using light-activated liposomes. The new method uses spherical nanostructures of fat molecules to carry CRISPR molecules to specific sites in the body.
A recent study published in Cell reveals that CRISPR/Cas9 genome editing can lead to undesirable outcomes, including the elimination of entire chromosomes or large sections in human embryos. The research warns against premature clinical application of this technology until further development and testing are conducted.
Researchers create genetic sensors that can detect gene activity, not just presence, using CRISPR-Cas13 system. This innovation has potential for biotech applications, including therapeutics and diagnostics.
The CRISPR Journal announces its October 2020 issue, featuring expert reactions to the National Academies' Heritable Human Genome Editing report. The journal also presents a comprehensive survey of global laws and regulations on hereditary human genome editing, highlighting both countries that prohibit and permit such research.
A genotype-agnostic gene therapy for cystic fibrosis has shown promise in clinical trials, potentially treating the disease in any patient, independent of their underlying mutation. Challenges remain to be overcome, including developing effective drug delivery systems that can reach pulmonary epithelial cells at low doses.
Researchers have developed two new genetic systems, e-CHACR and ERACR, to halt or eliminate the spread of gene drives in the wild. These systems use CRISPR technology to neutralize gene drives, which carry the power to immunize mosquitoes against malarial parasites or act as genetic insecticides.
Researchers have successfully applied CRISPR gene editing to influence the levels of beta-glucan in barley grain, with implications for brewing and distilling industries. The study provides insight into key genes responsible for barley grain composition, enabling plant breeders to accelerate breeding and develop new crop varieties.
Researchers developed a new tool to guide scientists in choosing the best CRISPR enzyme for their high-stakes gene edits, making the technology safer, cheaper and more efficient. The tool helps identify where mistakes are most likely to occur for each enzyme, saving time and reducing risk.
Researchers discovered that bacteria acquire spacers for their CRISPR 'database' by selecting snippets of bacteriophage's genetic information. This complex mechanism allows the bacteria to recognize and destroy invading viral genetic material.
Researchers created a system that uses CRISPR to briefly suppress genes related to AAV antibody production, allowing the virus to deliver its cargo unimpeded. The study shows promise for improving gene therapy's effectiveness and preventing or treating sepsis in mice.
Researchers from the University of Copenhagen have mapped and analyzed the atomic structure of the Cmr-β complex, a type III-B CRISPR-Cas system. The study provides new insights into the mechanisms behind this complex's immune response against phages and its potential therapeutic applications in fighting antibiotic resistance.
The new CGBE1 tool enables efficient induction of C-to-G mutations while minimizing unwanted changes, offering potential for treating disease-associated genetic mutations. Co-authors envision CGBE1 as useful for research applications, enabling introduction of specific C-to-G mutations.
A new hypercompact CRISPR enzyme, CasΦ, has been discovered in huge bacteriophages and provides a powerful tool for genome editing. It can target a wider range of genetic sequences than current CRISPR-Cas proteins, making it a promising alternative for cellular delivery.
A new study improves CRISPR gene editing by mutating the Cas9 enzyme to reduce off-target hits. The mutation increases fidelity up to 93-fold, making it a potentially safer strategy for gene therapy.
Scientists at Johns Hopkins Medicine have developed a light-activated CRISPR system that allows for targeted DNA cutting within seconds. The new technology reveals new details about the DNA repair process, which may aid in understanding aging and cancer.
A Spanish National Research Council (CSIC) project uses CRISPR tools to target and destroy the SARS-CoV-2 RNA genome in cells, potentially providing a new therapeutic approach. The researchers will test the functionality and non-toxicity of CRISPR reagents in zebrafish embryos before moving on to human viruses and infected cells.
A team of scientists from Stanford University has developed a gene-targeting, antiviral agent against COVID-19 using CRISPR technology. The system delivers PAC-MAN into lung cells, neutralizing the coronavirus and stopping it from replicating inside cells.
Scientists at ChristianaCare's Gene Editing Institute have developed a new CRISPR advance that can safely target and disable the NRF2 gene linked to a bleak prognosis in lung cancer tumors. This approach aims to improve the efficacy of conventional chemotherapy and radiation treatments while minimizing harm to normal cells.