Articles tagged with Crisprs
A new genome-editing strategy called DAP array can correct dozens of errors at the same time with high precision and efficiency, avoiding off-target edits. The technique leverages tRNA to drive multiple guide RNAs on a single array, then released individually by cells to direct genome editors for edits at multiple human genomic sites.
Researchers have developed a novel CRISPR-Cas9 method for gene editing in cockroaches, achieving efficiency rates of up to 22% and over 50% in the red flour beetle. The technique, named DIPA-CRISPR, allows for efficient and accessible gene editing without requiring expensive equipment or skilled laboratory personnel.
A team of researchers from Kumamoto University has developed a transformable polyrotaxane carrier that can facilitate genome editing using Cas9RNP with high efficiency. The carrier, called amino-PRX, is multi-step transformable and has low cytotoxicity, making it an enormously promising candidate for safe and efficient delivery.
Scientists at UC Riverside demonstrate CRISPR technology can make permanent physical changes in the insect, passed down to three or more generations. The technology may hold promise for controlling the sharpshooter and preventing Pierce's Disease.
Researchers used CRISPR gene editing to understand how deletions in one area of the genome affect nearby genes. They found that deleting a small region led to increased foetal globin expression and reduced adult globin levels, suggesting a key mechanism for asymptomatic patients with sickle cell disease.
Researchers at Northwestern University used CRISPR to identify human genes important for HIV infection in blood cells, finding 86 genes that may play a role in replication and disease. The study proposes a new map for understanding how HIV integrates into the DNA and establishes chronic infections.
Scientists have developed a new approach to expand the target range of CRISPR/Cas systems, allowing for slight variations in target DNA while maintaining local specificity. This technology could help realize the potential of CRISPR/Cas-based gene therapy and pathogen diagnosis, particularly for diagnostics.
Researchers found that gene duplications can complicate crop improvements, with some plants showing little effect despite similar mutations. This study highlights the need to understand evolutionary changes in plant genomes to develop more predictable crop improvements.
Researchers at ChristianaCare's Gene Editing Institute describe a new process for evaluating the impacts of gene edits that alter rather than completely disabling DNA code. The study validates the safety and efficacy of their novel approach for using CRISPR to improve lung cancer treatments.
Researchers have developed a vaccine using CRISPR technology to protect against Leishmania mexicana, a parasite causing cutaneous leishmaniasis in the Americas. The vaccine is safe and effective in mice, preventing skin lesions and infection for over 10 weeks.
Researchers developed a novel genetic barcode system to mark cancer cells with different gene modifications and image their characteristics. The Perturb-map platform identified specific genes controlling lung tumor growth, immune composition, and response to immunotherapy, offering new approaches for targeting anti-cancer drugs.
Scientists have developed powerful new methods for untangling and rewriting DNA, including a CRISPR-Cas12a toggle switch that can turn genes on and off. Nanopore tools are also being used to diagnose parasitic infections and sequence genes faster.
A team of paleogeneticists successfully sequenced the genome of the extinct Christmas Island rat, but found that key genes related to olfaction were missing. The study reveals the challenges and limitations of de-extinction across all species, highlighting the need for a more nuanced approach.
A Penn State-led team of researchers developed a new delivery system that improves the efficiency and lifespan of CRISPR gene-editing tools after delivery into stem cells. The method uses an enzyme called PiggyBac, which enables permanent integration of the editing tools into the cell's genetic code, resulting in 99% of mutated cells b...
Researchers at the University of Washington developed an AI-designed protein that can awaken individual dormant genes by disabling chemical 'off switches'. This approach allows for safe upregulation of specific genes to affect cell activity without permanently changing the genome.
Scientists at the University of Texas at Austin have redesigned a key component of the widely used CRISPR-based gene-editing tool Cas9 to be thousands of times less likely to target the wrong stretch of DNA. The new version, called SuperFi-Cas9, is as efficient as the original but reduces off-target interactions, making it potentially ...
Recent studies found that intestinal cells can change specializations in response to BMP signaling. This process, called zonation, is crucial for the proper functioning of the gut. Researchers used organoids and mouse models to confirm this discovery, which may lead to new treatments for metabolic diseases.
Researchers discovered a crucial RNA strand called CYTOR that helps build muscle mass, and found it decreases with age. Gene therapy stimulated CYTOR production, leading to increased fast-twitch muscle fibers and improved muscle function in humans and mice.
Scientists have developed a new therapy called CINDELA, which employs CRISPR-Cas9 to kill cancer cells while leaving normal tissues intact. The treatment targets specific mutations found in cancer cells and induces cell death through DNA double-strand breaks.
CROPSR, an open-source software tool, accelerates CRISPR experiment design and evaluation by addressing challenges in complex crop genomes. The genome-wide approach significantly shortens the time required to design a CRISPR experiment, reducing failed experiments.
Researchers introduce a genetic mutation that reduces Alzheimer's risk by preventing amyloid plaque formation. The mutation, found in Icelanders, has no known disadvantage and may be used to treat familial and sporadic forms of the disease.
A new, reliable kill switch has been developed to eliminate genetically modified microbes that pose environmental risks. By inserting multiple kill switches into the microbial DNA, a success rate of one in billion microbes was achieved during experiments.
Researchers at Gladstone Institutes and UC San Francisco have developed a CRISPR activation method that allows them to activate genes in human immune cells, revealing key regulators of cytokine production. This breakthrough accelerates immunotherapy research and may lead to more powerful cancer treatments.
A new study by Uppsala University researchers discovered that CRISPR-Cas9 can generate unexpected, heritable DNA mutations in zebrafish, highlighting the need for careful validation before using the method for medical purposes.
Scientists have developed a novel CRISPR-Cas3 editor from the bacteria Neisseria lactamica that improves editing efficiency and is more easily produced. The tool enables 50% editing efficiency in stem cells and 95% efficiency in other human cell lines, paving the way for research in genetic diseases and developmental biology.
Researchers at UCSF have identified a new potential drug target, BRD2, which regulates the ACE2 receptor, a key entry point for SARS-CoV-2. Blocking production of BRD2 prevents virus from infecting various human cell types.
A team of researchers at George Washington University identified a gene that determines whether ultraviolet iridescence appears in the wings of butterflies. Removing this gene from non-iridescent species leads to UV coloration in their wings, highlighting its critical role in evolutionary differences between species.
Researchers have developed a highly sensitive immuno-CRISPR assay to detect acute kidney rejection in urine, potentially allowing for early diagnosis without invasive biopsy. The test uses CRISPR/Cas12a gene editing technology to identify biomarkers of rejection, such as CXCL9, with improved sensitivity compared to existing methods.
Gladstone Institutes researchers have pioneered a new method to edit genes in human cells using retrons, which can produce abundant copies of template DNA from inside cells. The optimized system has shown improved efficiency and precision compared to current approaches.
Researchers have created a new approach to edit genes within specific bacteria in a community using CRISPR-Cas9, enabling targeted genetic modifications. This technology could be used to track edited microbes and potentially treat diseases like digestive issues or create more resilient crops.
Scientists at Oak Ridge National Laboratory developed a self-detect solution to monitor CRISPR gene editing tools in organisms. The system uses a biosensor guide RNA and reporter protein to trigger the technology's reveal itself, enabling real-time detection of CRISPR activity.
Researchers have developed a CRISPR/Cas9 gene editing system to enhance the effectiveness of sonodynamic therapy, allowing tumors to be effectively shrunk in a mouse model of liver cancer. The technology reduces antioxidant defense systems, increasing cancer cell death from the treatment.
Researchers at UC Berkeley have developed a new CRISPR editing technology that enables simultaneous editing of genes in multiple cell types and species within a microbial community. This approach, called community editing, has the potential to track edited microbes and understand their functions within complex ecosystems.
Researchers have expanded the number of naturally occurring CRISPR-Cas systems, giving a wealth of potential new tools for large-scale gene editing. The discovery could lead to treating complex diseases associated with multiple genes.
Researchers at Karolinska Institutet found that CRISPR gene-editing causes DNA damage, activating the p53 protein, which can lead to an accumulation of mutated cancer cells. The study identified a network of linked genes with similar mutations and suggests transient inhibition of p53 as a potential strategy to prevent their enrichment.
A recent study by Sanford Burnham Prebys and the National Cancer Institute has shown that CRISPR-Cas9 gene editing can favor cells with mutated forms of genes linked to cancer. This highlights the importance of monitoring patients undergoing CRISPR-based gene therapy for cancer-related mutations.
A new study published in Science Advances has shed light on the genetic basis of human appearance features by investigating the role of Hox genes. The researchers replaced the proboscipedia gene in a common laboratory fruit fly with its counterpart from a rarer Hawaiian cousin, revealing that Hox genes function as scaffolds for downstr...
Researchers used CRISPR gene-editing tools to show that a gene controlling bone growth in fish fins plays the same role in forming fingers and toes in four-legged creatures. The study suggests that the last common ancestor between ray- and lobe-finned fish already had the genetic toolkit to shape their appendages.
Researchers at UC San Diego have created a CRISPR-based rapid diagnostic technology that detects SARS-CoV-2, the coronavirus causing COVID-19. The new SENSR system is designed to simplify SARS-CoV-2 detection with a goal of eventual adaptation for in-home use.
A study by EPFL researchers reveals that CTCF sites within the HoxD cluster contribute to organizing genes into topologically associated domains, helping to organize developmental complexity. The dual function of CTCF binding sites varies depending on tissue type.
Researchers identify key neurons and signaling pathway regulating female activity before ovulation. Discoveries could lead to new treatments for menopause that sidestep estrogen and reactivates the circuit with CRISPRa technology.
A team of Harvard researchers created an integrated pipeline, STAMPScreen, to help genetic engineers identify target genes and perform screening studies. The protocol combines computational tools with lab experiments to quickly and efficiently test gene function in living cells.
Researchers at UC San Diego develop precision-guided sterile insect technique (pgSIT) to control mosquito populations. The system uses CRISPR to alter genes linked to male fertility and female flight in Aedes aegypti, reducing disease transmission.
Researchers at UMD are developing CRISPR-Combo systems to improve genome editing and crop regeneration. The technology aims to reduce the time and cost of breeding new crop varieties with enhanced nutritional and agronomic traits.
Stanford researchers have developed a mini CRISPR genome editing system that is smaller and more efficient than existing versions. The new system, called CasMINI, has been successfully tested in human cells and shows promise for treating various diseases, including eye disease, organ degeneration, and genetic diseases.
A compact CasMINI CRISPR system has been engineered for efficient gene regulation and editing in mammalian cells. The system, derived from the naturally occurring archaeal protein Cas12f, is highly specific and efficient, making it suitable for various therapeutic applications.
The new MIC-Drop technology enables rapid evaluation of hundreds of genes in a single experiment, improving large-scale genetic studies. Researchers can identify essential genes for healthy heart development and function.
A CRISPR screening tool identified ZMYND8, an epigenetic regulatory protein, as a potential new therapeutic target for acute myeloid leukemia. Inhibiting ZMYND8 has been shown to leave cancer cells with smaller tumors and better survival in mouse models.
Scientists have repurposed CRISPR to identify antibodies in patient blood samples, demonstrating a new class of medical diagnostics. The technique uses customizable proteins attached to Cas9, which assemble on a microchip to bind to specific DNA sequences, allowing for fast and accurate detection.
Researchers at UC Berkeley created a rapid COVID-19 diagnostic test utilizing tandem CRISPR nucleases, which accelerates RNA detection and reduces sample handling. This innovation simplifies the assay process, making it faster and more efficient for SARS-CoV-2 virus detection.
Researchers have mapped the structure of CRISPR-Cas12j3 from bacteriophages, a discovery that reveals how it works and solves packaging problems for genome editing. The new system has vast potential for precise genome editing with improved efficiencies and alternative targeting mechanisms.
Researchers at the University of Oregon used CRISPR-Cas9 gene editing to target a specific mutation causing Fuchs' corneal dystrophy, preserving endothelial cell density and function. The study lays the groundwork for future research on using this technique to treat genetic disorders in post-mitotic cells.
Researchers developed chemically modified guide RNAs for an RNA-targeting CRISPR system, significantly enhancing the ability to target and edit RNA in human cells. The optimized guides increased efficiencies of CRISPR activity by 2- to 5-fold and extended targeting activity from 48 hours to four days.
Yiping Qi's team will test new delivery technologies for CRISPR-Cas12a to develop a pipeline for genome editing in carrots. They aim to create more nutritious and hypoallergenic carrot varieties with improved nutritional value and reduced allergenicity.
The new CRISPR 3.0 system allows for simultaneous activation of up to seven genes at once, demonstrating high accuracy and efficiency. This multiplexed gene activation system has significant potential for crop breeding and enhancement, enabling the discovery and translational science in plants.
Researchers have successfully catalogued the effect of individual genes on human neuron function and survival, revealing unexpected results. The team's findings suggest that switching off certain genes can lead to an increase in oxidative stress, which may contribute to neurodegenerative diseases.
The study created a large-scale atlas of chromatin accessibility changes with the loss of individual enzymes, revealing how DNA is organized in cancer cells. Chromatin modifications are linked to diverse human traits and diseases, most notably cancer, where their loss results in global gene expression changes.
The use of CRISPR technology demands more robust public engagement to harness its benefits without crossing ethical lines. Effective communication that incorporates social science research can facilitate nuanced debates about the societal implications of genetic editing.
Scientists at Beam Therapeutics have created a redesigned base editor that successfully repairs the single-base mutation causing sickle-cell disease. The therapy targets an upstream regulatory pathway to express fetal hemoglobin, offering a potential solution for this genetic disorder.
Scientists have developed a novel CRISPR-based tool called CRISPRoff, which allows for the silencing of almost any gene in human cells without making DNA edits. This technology has significant therapeutic potential, particularly for rare genetic disorders that are caused by a single damaged copy of a gene.