Articles tagged with Crisprs
Researchers have successfully treated six children with relapsed and treatment-resistant B-ALL using CRISPR-edited T cells, achieving deep remission in four patients. The treatment has shown promise as a viable alternative to currently available treatments.
Researchers have developed a pioneering gene editing strategy that can repair faulty genes in immune cells, offering new hope for patients with conditions like CTLA-4 insufficiency. The technique uses CRISPR/Cas9 to target and correct the faulty gene, preserving important regulatory mechanisms.
Researchers at VCU Massey Cancer Center have identified a new therapeutic target for triple-negative breast cancer (TNBC), a more aggressive and deadly form of disease. Using CRISPR/CAS9 screening, scientists pinpointed the UBA1 enzyme as an ideal target, which can be inhibited by the novel drug TAK-243 to effectively kill cancer cells.
Researchers at Rice University have developed a procedure to quantify unintended changes that accompany on-target CRISPR-Cas9 gene editing, potentially threatening the efficacy and safety of therapies. The new method uses single-molecule sequencing with unique molecular identifiers to detect large deletions, insertions, and chromosomal...
A new CRISPR-Cas approach, SpRYgests, allows for precise DNA cleavage at any sequence, expanding the utility of molecular cloning approaches. This innovation simplifies and expedites DNA editing and has potential clinical implications.
The study found that microbial enzymes are essential for the digestion of pectin in leaf beetles, allowing them to access nutrient-rich plant cells. The researchers also discovered that leaf beetle species acquire these enzymes through horizontal gene transfer from other microbes.
Researchers at Northwestern University developed a new CRISPR-based therapy platform that can deliver cargo to a broader range of tissue and cell types, increasing its potential for treating various diseases. The platform achieves this by transforming the Cas-9 protein into a spherical nucleic acid and loading it with critical components.
Brown algae's unique pigments have evolved through a complex genetic pathway, enabling them to harness more light energy than green plants. This discovery could lead to insights into fucoxanthin's health applications and improved photosynthetic efficiency for biofuels production.
Researchers at KIT have developed a method to suppress nine-tenths of a chromosome using CRISPR/Cas molecular scissors, preventing genetic exchange and allowing positive traits to be passed on together. This technique can improve the efficiency of crop breeding by combining favorable traits in one plant.
Researchers at Gladstone Institutes and UCSF have developed a new approach to introduce long DNA sequences into cells with remarkable efficiency. The technology, which uses single-stranded DNA templates, overcomes the limitations of traditional viral vectors and has the potential to make cell therapies faster, better, and less expensive.
Researchers at Cornell University have discovered a new CRISPR system called Craspase, which has the potential to develop promising antiviral and tissue engineering tools in animals and plants. The study uses cryo-electron microscopy snapshots to explain how Craspase identifies RNA targets and activates proteases.
Scientists at UC San Francisco and Gladstone Institutes use CRISPR-based edit to render therapeutic T cells more resilient, overcoming a major factor limiting cancer immunotherapies' success. The discovery may help improve treatment of both solid and liquid tumors.
Researchers at the University of California San Diego have developed a precision-guided sterile insect technique (pgSIT) to control invasive fruit fly populations. The technology uses CRISPR editing to target key genes in female viability and male fertility, resulting in a fertility dead end for the species.
A team of researchers from Princeton University and the Broad Institute created a fast, accurate, and equipment-free diagnostic test for COVID-19. The test uses CRISPR technology to detect SARS-CoV-2 variants and can be performed at home without special equipment, improving sensitivity and specificity compared to existing tests.
Researchers at UC San Francisco have discovered how to shift damaged brain cells from a diseased state into a healthy one using CRISPR technology. The study found that reprogramming microglia cells can help remove protein plaques and protect synapses, potentially treating Alzheimer's and other forms of dementia.
Researchers at IRB Barcelona have found that CRISPR/Cas9 gene editing can trigger cell toxicity and genomic instability, particularly in regions near the tumour suppressor protein p53. The study identified 3,300 targeted spots with strong toxic effects, highlighting the need for safer CRISPR reagents.
Researchers at Gladstone Institutes developed a tool called Retro-Cascorder, which logs a cell's genetic activity for days at a time. This allows scientists to create living biosensors that can record changes to their environment.
A new study from Tel Aviv University found that CRISPR therapeutics can lead to a significant loss of genetic material in treated cells, potentially destabilizing the genome and promoting cancer. The researchers detected up to 10% of cells with lost chromosomes, highlighting the need for extra care when using this technology.
NC State researchers discovered a new way to make the difficult-to-characterize gut bacterium Bifidobacterium more responsive to antibiotics. They also found tiny changes in different strains that reflect large differences in their characteristics, highlighting the need for individualized CRISPR-based genome engineering approaches.
Loss of the Y chromosome in white blood cells causes fibrosis in the heart, impaired heart function, and death from cardiovascular diseases in men. Men with this genetic change have an increased risk of developing age-related diseases such as cancer and Alzheimer's disease.
Rice University bioscientists have developed a novel approach to control the expression of 'silent' genes in bacteria using CRISPR technology. This strategy could lead to the discovery of new antibiotics and has potential applications in antifungal and anticancer agents, as well as agriculture.
Experimental study finds large DNA insertions caused by retrotransposition can increase cancer risk in human cells edited with CRISPR/Cas9. In contrast, base editing and prime editing show much lower rates of retrotransposition.
A new CRISPR strategy, employing natural DNA repair machinery, provides a foundation for novel gene therapy strategies to cure genetic diseases. The technique, known as homologous chromosome-templated repair, uses "nicks" of single DNA strands to correct genetic defects.
A Danish research study has made significant discoveries about the CRISPR/Cas9 protein and its gRNA component, enabling more accurate gene modification. The findings aim to optimize the effectiveness of gene editing, addressing concerns over 'off-target effects'.
A new study from Tel Aviv University developed a unique treatment for AIDS that may be developed into a vaccine or a one time treatment. The treatment involves engineering type B white blood cells to secrete anti-HIV antibodies in response to the virus.
Bacteria's CRISPR system uses spacers to store viral information, but must balance risk of autoimmunity with immune memory. Longer spacers reduce autoimmune response risk, allowing more spacers to be stored without triggering an immune reaction.
Researchers used CRISPR technology to modify the ratio of amylose and amylopectin in potatoes, increasing culinary and industrial uses. The study found that potatoes with high amylopectin content have potential applications in bioplastics, food additives, adhesives, and ethanol production.
Scientists from the University of Maryland developed CRISPR-Combo, a method to edit multiple genes in plants while simultaneously changing gene expression. This new tool enables genetic engineering combinations that work together to boost functionality and improve breeding of new crops.
A new genome-editing strategy called DAP array can correct dozens of errors at the same time with high precision and efficiency, avoiding off-target edits. The technique leverages tRNA to drive multiple guide RNAs on a single array, then released individually by cells to direct genome editors for edits at multiple human genomic sites.
Researchers have developed a novel CRISPR-Cas9 method for gene editing in cockroaches, achieving efficiency rates of up to 22% and over 50% in the red flour beetle. The technique, named DIPA-CRISPR, allows for efficient and accessible gene editing without requiring expensive equipment or skilled laboratory personnel.
A team of researchers from Kumamoto University has developed a transformable polyrotaxane carrier that can facilitate genome editing using Cas9RNP with high efficiency. The carrier, called amino-PRX, is multi-step transformable and has low cytotoxicity, making it an enormously promising candidate for safe and efficient delivery.
Scientists at UC Riverside demonstrate CRISPR technology can make permanent physical changes in the insect, passed down to three or more generations. The technology may hold promise for controlling the sharpshooter and preventing Pierce's Disease.
Researchers used CRISPR gene editing to understand how deletions in one area of the genome affect nearby genes. They found that deleting a small region led to increased foetal globin expression and reduced adult globin levels, suggesting a key mechanism for asymptomatic patients with sickle cell disease.
Researchers at Northwestern University used CRISPR to identify human genes important for HIV infection in blood cells, finding 86 genes that may play a role in replication and disease. The study proposes a new map for understanding how HIV integrates into the DNA and establishes chronic infections.
Scientists have developed a new approach to expand the target range of CRISPR/Cas systems, allowing for slight variations in target DNA while maintaining local specificity. This technology could help realize the potential of CRISPR/Cas-based gene therapy and pathogen diagnosis, particularly for diagnostics.
Researchers found that gene duplications can complicate crop improvements, with some plants showing little effect despite similar mutations. This study highlights the need to understand evolutionary changes in plant genomes to develop more predictable crop improvements.
Researchers at ChristianaCare's Gene Editing Institute describe a new process for evaluating the impacts of gene edits that alter rather than completely disabling DNA code. The study validates the safety and efficacy of their novel approach for using CRISPR to improve lung cancer treatments.
Researchers have developed a vaccine using CRISPR technology to protect against Leishmania mexicana, a parasite causing cutaneous leishmaniasis in the Americas. The vaccine is safe and effective in mice, preventing skin lesions and infection for over 10 weeks.
Researchers developed a novel genetic barcode system to mark cancer cells with different gene modifications and image their characteristics. The Perturb-map platform identified specific genes controlling lung tumor growth, immune composition, and response to immunotherapy, offering new approaches for targeting anti-cancer drugs.
A team of paleogeneticists successfully sequenced the genome of the extinct Christmas Island rat, but found that key genes related to olfaction were missing. The study reveals the challenges and limitations of de-extinction across all species, highlighting the need for a more nuanced approach.
Scientists have developed powerful new methods for untangling and rewriting DNA, including a CRISPR-Cas12a toggle switch that can turn genes on and off. Nanopore tools are also being used to diagnose parasitic infections and sequence genes faster.
A Penn State-led team of researchers developed a new delivery system that improves the efficiency and lifespan of CRISPR gene-editing tools after delivery into stem cells. The method uses an enzyme called PiggyBac, which enables permanent integration of the editing tools into the cell's genetic code, resulting in 99% of mutated cells b...
Researchers at the University of Washington developed an AI-designed protein that can awaken individual dormant genes by disabling chemical 'off switches'. This approach allows for safe upregulation of specific genes to affect cell activity without permanently changing the genome.
Scientists at the University of Texas at Austin have redesigned a key component of the widely used CRISPR-based gene-editing tool Cas9 to be thousands of times less likely to target the wrong stretch of DNA. The new version, called SuperFi-Cas9, is as efficient as the original but reduces off-target interactions, making it potentially ...
Recent studies found that intestinal cells can change specializations in response to BMP signaling. This process, called zonation, is crucial for the proper functioning of the gut. Researchers used organoids and mouse models to confirm this discovery, which may lead to new treatments for metabolic diseases.
Researchers discovered a crucial RNA strand called CYTOR that helps build muscle mass, and found it decreases with age. Gene therapy stimulated CYTOR production, leading to increased fast-twitch muscle fibers and improved muscle function in humans and mice.
Scientists have developed a new therapy called CINDELA, which employs CRISPR-Cas9 to kill cancer cells while leaving normal tissues intact. The treatment targets specific mutations found in cancer cells and induces cell death through DNA double-strand breaks.
CROPSR, an open-source software tool, accelerates CRISPR experiment design and evaluation by addressing challenges in complex crop genomes. The genome-wide approach significantly shortens the time required to design a CRISPR experiment, reducing failed experiments.
Researchers introduce a genetic mutation that reduces Alzheimer's risk by preventing amyloid plaque formation. The mutation, found in Icelanders, has no known disadvantage and may be used to treat familial and sporadic forms of the disease.
A new, reliable kill switch has been developed to eliminate genetically modified microbes that pose environmental risks. By inserting multiple kill switches into the microbial DNA, a success rate of one in billion microbes was achieved during experiments.
Researchers at Gladstone Institutes and UC San Francisco have developed a CRISPR activation method that allows them to activate genes in human immune cells, revealing key regulators of cytokine production. This breakthrough accelerates immunotherapy research and may lead to more powerful cancer treatments.
A new study by Uppsala University researchers discovered that CRISPR-Cas9 can generate unexpected, heritable DNA mutations in zebrafish, highlighting the need for careful validation before using the method for medical purposes.
Scientists have developed a novel CRISPR-Cas3 editor from the bacteria Neisseria lactamica that improves editing efficiency and is more easily produced. The tool enables 50% editing efficiency in stem cells and 95% efficiency in other human cell lines, paving the way for research in genetic diseases and developmental biology.
Researchers at UCSF have identified a new potential drug target, BRD2, which regulates the ACE2 receptor, a key entry point for SARS-CoV-2. Blocking production of BRD2 prevents virus from infecting various human cell types.
A team of researchers at George Washington University identified a gene that determines whether ultraviolet iridescence appears in the wings of butterflies. Removing this gene from non-iridescent species leads to UV coloration in their wings, highlighting its critical role in evolutionary differences between species.
Gladstone Institutes researchers have pioneered a new method to edit genes in human cells using retrons, which can produce abundant copies of template DNA from inside cells. The optimized system has shown improved efficiency and precision compared to current approaches.
Researchers have developed a highly sensitive immuno-CRISPR assay to detect acute kidney rejection in urine, potentially allowing for early diagnosis without invasive biopsy. The test uses CRISPR/Cas12a gene editing technology to identify biomarkers of rejection, such as CXCL9, with improved sensitivity compared to existing methods.
Researchers have created a new approach to edit genes within specific bacteria in a community using CRISPR-Cas9, enabling targeted genetic modifications. This technology could be used to track edited microbes and potentially treat diseases like digestive issues or create more resilient crops.
Scientists at Oak Ridge National Laboratory developed a self-detect solution to monitor CRISPR gene editing tools in organisms. The system uses a biosensor guide RNA and reporter protein to trigger the technology's reveal itself, enabling real-time detection of CRISPR activity.
Researchers have developed a CRISPR/Cas9 gene editing system to enhance the effectiveness of sonodynamic therapy, allowing tumors to be effectively shrunk in a mouse model of liver cancer. The technology reduces antioxidant defense systems, increasing cancer cell death from the treatment.