Articles tagged with Crisprs
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
A new study found that a 'courtship' gene has different effects in two fruit fly species. In one species, giving females the gene resulted in them adopting male behaviors, while in another, it enabled them to produce both male and female songs. The findings suggest that genes can have varying functions across different species.
Researchers successfully demonstrate CRISPR-Cas gene editing technology to eliminate all traces of the HIV virus from infected cells in laboratory settings. The study aims to develop a robust and safe combinatorial regimen to target diverse HIV strains across various cellular contexts.
A new link has been discovered between FBXW7 mutations and EGFR signaling activity in colorectal cancer. The study found that the mutated form of the FBXW7 gene could no longer degrade the EGFR protein, leading to increased signaling activity and a decreased response to anti-EGFR treatment.
Tulane University researchers have developed a CRISPR-based platform for diagnosing nontuberculous mycobacteria (NTM) infections, allowing for accurate results in as little as two hours. The blood test can identify over 93% of patients with an NTM infection, enabling rapid treatment plans and reducing the risk of complications.
Researchers have discovered new genetic mechanisms related to spinocerebellar ataxia type 37, a rare neurological disorder that affects balance and movement. The study employed advanced techniques such as CRISPR/Cas9 gene editing and machine learning to uncover the disease's underlying causes.
A team of researchers used CRISPR-Cas9 gene editing to enhance the nutritional profile and flavor of fungi, creating a new source of plant-based food alternatives. The modified fungi produce heme and ergothioneine, which can improve cardiovascular health benefits.
Researchers create a technique using prime editing to quickly and easily screen cancer genes, revealing new information on p53 mutations. The method allows for the analysis of over 1,000 different mutations in the tumor suppressor gene p53, which are seen in more than half of all cancer patients.
Researchers at CABBI developed a computational pipeline for identifying CRISPR/Cas-facilitated integration sites, which can pinpoint neutral integration sites in two to three minutes. This tool enables researchers to efficiently locate all the needles that align with their specific criteria, transforming the genome editing process.
Researchers have developed a gene editing technique that can repair defective immune cells using CRISPR-Cas9, showing promise in treating rare diseases like Familial Hemophagocytic Lymphohistiocytosis. The therapy involves repairing genetic defects in cytotoxic T cells to normalize the immune response.
A breakthrough gene-editing therapy has been successfully treated over ten patients with hereditary angioedema, reducing symptoms by up to 95% and potentially providing a permanent cure. The therapy targets the KLKB1 gene responsible for producing plasma prekallikrein.
A new CRISPR delivery method enables precise targeting of specific cell subsets in living animals, paving the way for programmable gene therapy. The system uses antibody-targeted 'enveloped delivery vehicles' to selectively edit T-cells and create CAR T-cells.
Researchers have developed CRISPR off-switches to mitigate off-target effects, a major concern in genome editing. The new technology, based on anti-CRISPR proteins, can block CRISPR-Cas3 machine function and prevent unintended edits.
The pAblo·pCasso technology offers unprecedented precision and flexibility in genetic engineering, enabling rapid and precise genetic modifications of bacteria. This breakthrough expands the range of possible genomic editing sites, allowing for temporary modifications and dynamic gene studies.
Researchers from HIRI have developed a new machine learning approach using data integration and AI to improve predictions of CRISPRi efficacy, revealing that gene features matter more than guide RNA itself. The study provides valuable insights for designing effective CRISPRi experiments.
A new study has identified three genes, MANBA, TNFRSF13B, and EEF1A1, as crucial in the regulation of IgG galactosylation, a trait associated with ageing. The research used GWAS to analyze IgG glycosylation phenotypes in a large sample size, increasing the understanding of this complex posttranslational modification.
Recent progress in CRISPR-Cas editing enables tailored probiotic organisms to promote gut health, support immune systems, and enhance metabolism. Genetically modified probiotics have shown potential in preventing or mitigating diseases, such as antibiotic-resistant bacteria and inflammatory bowel disease.
Recent advances in CRISPR-Cas genome engineering enable the creation of novel probiotic strains with potential treatments for various diseases. Genetically modified probiotics show great promise in treating cancers, inflammatory bowel disease, and obesity, while combating antibiotic resistance.
A team of researchers has developed a promising gene-editing strategy for spinal muscular atrophy (SMA), a devastating pediatric neuromuscular disorder. The approach involves using CRISPR base editing to activate the SMN2 gene, which is similar to the mutated SMN1 gene responsible for SMA.
Researchers discovered a novel family of effector proteins called Cami1 that inhibit translation in bacteria attacked by viruses. By cleaving specific mRNAs, Cami1 prevents the production of viral proteins, allowing the bacterium to conserve resources.
Researchers discuss CRISPR's limitations in generating accurate cancer models, including variable mutations and indels. Despite these challenges, the technology holds promise for cancer research due to its potential for natural selection and Darwinian evolution.
Researchers at the University of Copenhagen discovered that phages use small RNAs to disarm bacterial CRISPR-Cas immune systems, making them vulnerable to infection. This finding has significant implications for phage therapy and could lead to more specific and controlled CRISPR-Cas treatments.
Scientists at Florida State University produced the first high-resolution images showing magnesium ions playing a crucial role in CRISPR-Cas9's DNA-cutting process. The discovery sheds light on how magnesium coordinates double-stranded breaks, providing new insights into the enzyme's functioning.
A novel replacement strategy using CRISPR-Cas9 and recombinant DNA donor vectors is proposed to precisely replace disease-causing genes in SCID patients. This approach preserves regulatory elements and intronic sequences, reducing the risk of unregulated gene expression.
Researchers used gut organoids to study gut cell differentiation, identifying ZNF800 as a key regulator of enteroendocrine cells. The discovery could have implications for understanding gastrointestinal diseases and endocrine disorders.
Researchers have developed a novel approach, REVeRT, to efficiently transport large genes using dual AAV vectors at the transcript level. This new method offers increased efficiency, fewer side effects, and greater flexibility compared to existing strategies.
Researchers have identified a new mechanism by which phages evade CRISPR-Cas immune systems in bacteria, revealing a potential approach to make gene editing safer and more efficient. This discovery could lead to the development of bespoke anti-CRISPRs to neutralize CRISPR-Cas systems and provide an alternative to antibiotics.
Researchers have developed a new method to study muscle diseases, reducing the number of experimental animals needed. This method enables the simultaneous investigation of several genes or entire signaling pathways in muscle fibers quickly and efficiently.
Researchers created a new CRISPR-based gene therapy tool using locally sourced, human-derived proteins that can activate silent or insufficiently expressed genes. The DREAM tool mimics the natural ability of human cells to turn on specific genes in response to mechanical cues.
Researchers at Kyoto University discovered that liverwort Marchantia polymorpha uses gibberellin precursors to produce a signaling molecule aiding survival under shaded conditions. This metabolic pathway inheritance provides insight into the evolution of plant hormone responses.
A new CRISPR-based gene-editing tool, AsCas12f, has been developed with enhanced editing ability and compact size. The engineered enzyme has already shown success in animal trials and holds promise for improved treatments of genetic disorders.
A new cell model has been created to simulate the evolution of a common form of childhood leukemia, B-cell acute lymphoblastic leukaemia. The model replicates the disease in children's cells, providing a crucial tool for researchers to develop new therapeutic strategies.
Rice University scientists developed a tiny CRISPR-Cas13 system to shred viruses by targeting RNA. The system's unique mechanism and three-dimensional structure were mapped using cryo-electron microscopy, allowing researchers to engineer it for improved precision and specificity.
Researchers at Rice University have developed a non-invasive gene delivery technique using ultrasound to efficiently deliver clinically used gene therapy vectors throughout the brain. The study, published in Gene Therapy, shows that opening more sites within targeted regions improves gene delivery efficiency.
Researchers create adenine base editor with 'on/off' switch, reducing off-target edits by over 70% and increasing accuracy of on-target edits. The tool has potential to correct nearly half of disease-causing point mutations in human genome.
Researchers at ETH Zurich have created a method to simultaneously modify multiple genes in individual cells of an animal, allowing for the study of complex diseases. By precisely analyzing these cells, scientists can identify key genes responsible for disease progression, paving the way for potential drug development.
A new CRISPR-based diagnostic tool, MPXV-CRISPR, has been developed in Australia to detect the monkeypox virus with high precision and speed. The tool can detect the virus in clinical samples in just 45 minutes, making it faster than current methods.
Researchers at Osaka University have developed a new gene editing technique called NICER, which significantly reduces off-target mutations compared to traditional CRISPR/Cas9 methods. This novel approach uses multiple small cuts in DNA strands and promotes interhomolog homologous recombination to correct heterozygous mutations.
Researchers developed a technology to rapidly screen genetic edits in immune cells, identifying a new combination that improves their effectiveness against cancers. By combining multiple genes into long DNA stretches and testing thousands of combinations, scientists discovered that different CARs can be optimized by different factors.
Researchers analyzed Wikipedia's CRISPR-related articles to understand the site's role in documenting scientific history. The study found that Wikipedia's content reflects global conversations on cutting-edge technologies like CRISPR.
Researchers at Gladstone Institutes identified conditions that enable gamma delta T cells to recognize cancer cells by disrupting energy production and causing cellular stress. This insight suggests that therapies manipulating butyrophilin abundance on the surface of cancer cells could boost gamma delta T cell effectiveness.
CyDENT base editors allow efficient and precise modification of genetic information in living organisms. The system enables strand-specific base editing in nuclear and organellar genomes, with high strand specificity demonstrated in mitochondrial genome editing.
A CRISPR-Cas3 system has restored dystrophin protein function in induced pluripotent stem cells from patients with Duchenne muscular dystrophy. The approach uses a dual CRISPR RNA method to remove large sections of the dystrophin gene, yielding truncated but still functional proteins for various mutation patterns.
Researchers used base editors to introduce specific combinations of activating and inactivating mutations into healthy organoids, creating realistic models for various types of cancer. This allows for further investigation into the development and treatment of cancer, with potential applications including testing new drugs.
A novel CRISPR-based gene-editing treatment, EBT-001, effectively removes SIV from the genomes of non-human primates without off-target effects. The study's findings support the development of a cure for HIV/AIDS in humans and pave the way for ongoing clinical trials.
Researchers have engineered bacteria that can detect tumor DNA in a live organism, using CRISPR technology. The bacteria, Acinetobacter baylyi, were designed to respond to specific DNA sequences associated with cancer, allowing for early detection and potentially preventing disease progression.
Researchers at the Marine Biological Laboratory have devised a method to precisely alter rotifer genomes using CRISPR-Cas9, enabling the study of fundamental biology and evolution. The new approach will allow scientists to investigate various aspects of biology, including aging, DNA repair mechanisms, and mitochondrial function.
Researchers at A*STAR and NUS Medicine developed a CRISPR-Cas13 therapeutic that directly targets and eliminates EV-A71 RNA viruses in laboratory models. The treatment shows potent reduction of viral burden, clearing infection and preventing organ damage and mortality.
Researchers successfully modified the ethylene synthesis pathway in the Japanese luxury melon to increase its shelf-life. The study found that introducing a mutation into the CmACO1 gene reduced ethylene generation, resulting in firmer fruit and longer shelf life.
Researchers at Cornell University have discovered a mutation in the MdLAZY1A gene responsible for the 'weeping' growth pattern in apple trees. This finding could lead to more productive and labor-saving orchards by allowing branches to grow downwards, thereby increasing resource allocation towards reproductive growth.
Gang Bao's lab receives a 4-year, $2.6 million grant from the National Institutes of Health to investigate the safety and efficacy of using gene editing treatments like CRISPR-Cas9 to treat sickle cell disease. The team aims to understand the mechanisms behind large gene modifications and their biological consequences.
Hematopoietic stem cell culture technology improves genome editing in HSCs by increasing successful correction rates to 100%, eliminating genetic mutations, and enhancing cell transplantation outcomes. This breakthrough enhances the efficiency and safety of gene editing in treating genetic diseases.
Scientists have developed a new genetic technology called Ifegenia that suppresses populations of Anopheles gambiae mosquitoes, which primarily spread malaria in Africa. The system targets females, which are the primary disease carriers, and kills them, halting parasite transmission.
A new study from Aarhus University has found that applying AI predictions of protein structures enhances the CRISPR technology, making the cuts in a patient's DNA more precise. This discovery may lead to better treatments for patients with genetic disorders and potentially develop cures for various genetic diseases.
Researchers have discovered that gene editing technologies may introduce unintended mutations and damage to DNA in early human embryos. The study found that most cells repair breaks in the DNA using non-homologous end joining, which can lead to additional genetic abnormalities.
Researchers used a stem cell model to study the effects of Alzheimer's disease-associated mutations on early human brain development, finding that mutant spheres were larger and contained fewer mature neurons. The study highlights the need for tailored therapies and paves the way for studying Alzheimer's in its early stages.
A study reports a CRISPR/Cas9 delivery system that reduces anxiety-related behavior in mice by modulating neuronal receptor pathways. The treatment, delivered through the nose, shows a 35.7% increase in time spent in light areas and a 14.8% decrease in marble burial compared to controls.
Researchers successfully engineered an albino strain of the hummingbird bobtail squid, offering clear optical access for visualizing its nervous system. This breakthrough presents Euprymna berryi as a viable candidate for a model cephalopod, enabling scientists to study complex animal behavior and unlock secrets of biology.
A team of scientists used CRISPR-Cas genome editing to create a disease-resistant rice variety, which showed high yields and resistance to the fungus that causes rice blast. The new rice variety produced five times more yield than the control rice in small-scale field trials.
Researchers developed a CRISPR/Cas9-based gene drive system targeting the Drosophila suzukii doublesex gene, resulting in female sterility and high transmission rates. Mathematical modeling predicts efficient population suppression with low release ratios.
Researchers at Osaka University developed a highly reproducible genetic screen to investigate Toxoplasma's survival within hosts. The study identified IFN-γ-dependent and -independent virulence factors that promote parasite fitness, providing potential targets for treatment and prevention of toxoplasmosis.