Articles tagged with Crisprs
Researchers at the Salk Institute have identified dozens of microproteins that play a crucial role in regulating fat cell proliferation and lipid accumulation. This breakthrough discovery offers new potential drug targets for treating obesity and metabolic disorders, building on recent advances in CRISPR gene editing technologies.
A team of scientists proposes an integrated framework combining biotechnology and AI to revolutionize crop breeding, exploring multi-omics, genome editing, and high-throughput phenotyping. The authors present a forward-looking framework for AI-assisted crop germplasm design, offering a roadmap for sustainable agriculture.
Researchers developed a CRISPR-based gene-editing system that changes a single molecule within mosquitoes, halting malaria-parasite transmission. The new system is designed to genetically spread the malaria resistance trait until entire populations of the insects no longer transfer the disease-causing parasites.
A team of scientists proposes using gene editing to restore lost genetic diversity in endangered species, enabling them to adapt to future environmental changes. The approach could complement traditional conservation methods and attract new investors and expertise.
Researchers successfully modulate the activity of key immune system genes using CRISPR-Cas9 technology, revealing a new approach to treating inflammatory diseases. The study demonstrates precise control over gene expression and its impact on tumour growth and inflammation.
Scientists have identified a set of genes in zebrafish that reactivate after damage to the heart and patch it up like new. The researchers hope to use CRISPR tools to reactivate similar genes in humans and jump-start repair of the heart and other tissues after injury.
The Center will develop personalized CRISPR on-demand treatments for children with severe inborn errors of immunity and metabolic disease. The initiative aims to make CRISPR cures more affordable and accessible, building on recent clinical success in treating ultra-rare genetic diseases.
Researchers developed Variant-aware Cas-OFFinder, a web-based tool that improves CRISPR accuracy by identifying off-target effects across genetic variations. The tool offers a significant step forward in personalized genome editing by incorporating genetic diversity directly into off-target predictions.
Researchers have successfully edited harmful mitochondrial DNA mutations in liver and skin cells using a genetic tool called a base editor. The study, published in PLOS Biology, offers promising results for treating mitochondrial diseases and aging-related conditions.
Researchers developed a new laboratory method that helps diagnose patients with suspected immune system disorders like activated-PI3Kδ syndrome (APDS). The method identified dozens of additional genetic variations associated with the condition, enabling rapid diagnosis and treatment. This breakthrough may also help identify more patien...
Researchers have found a promising new method for gene therapy by bringing dormant genes closer to enhancer switches on the DNA. This 'delete-to-recruit' strategy has potential for treating genetic diseases such as sickle cell disease and beta-thalassemia, offering an alternative to expensive current treatments.
Researchers at KAIST have developed a groundbreaking technology capable of selectively acetylating specific RNA molecules within the human body using the CRISPR-Cas13 system. This breakthrough enables precise, programmable control of RNA function and is expected to open new avenues in RNA-based therapeutic development.
Researchers leveraged CRISPR/CasRx to silence Ctnnb1 and Smo, two genes involved in osteoarthritis development. Simultaneous knockdown of both genes effectively mitigated joint structure degeneration, highlighting the potential of RNA-based gene therapies for OA management.
Researchers discovered that a ligase called Lig3 inhibits base editing, while the mismatch repair pathway helps cytosine base editing. The study sheds light on the complex mechanisms behind base editing and its potential applications in treating genetic diseases.
Researchers at Stanford University have developed a new CRISPR technology called CRISPR-TO that can transport RNA molecules to specific locations within neurons, enabling repair and regeneration. The technology has shown promising results in increasing neurite growth by up to 50% in mouse brain neurons.
Researchers at MSK uncovered a key signaling molecule involved in the body's immune response against leptomeningeal metastasis. A new grading system to assess thrombocytopenia risk after CAR T cell therapy was also developed. Additionally, a statistical method called UnitedMet estimates metabolic characteristics from challenging clinic...
Scientists at Rockefeller University have identified a novel CARF effector called Cat1 that prevents viral replication by depleting NAD+ metabolites. The discovery sheds new light on the complex molecular mechanisms behind CRISPR-Cas9 defense systems.
A new generative AI technique allows for the design of RNA molecules with improved functions, opening up potential for novel therapeutics and diagnostics. The SANDSTORM and GARDN systems enable the prediction and generation of RNA sequences tailored for specific tasks in cells or diagnostic assays.
Researchers at Karolinska Institutet have developed a technique to deliver gene editors and protein therapeutics to cells using engineered extracellular vesicles. The method shows promising results in animal studies, highlighting the potential for treating genetic diseases and neurological disorders.
Mass General Brigham researchers developed a machine learning algorithm, PAMmla, to predict properties of genome editing enzymes. The approach helps reduce off-target effects and improves editing safety and efficiency, enabling customized enzymes for new therapeutic targets.
Tulane University scientists developed a handheld device to deliver rapid and accurate tuberculosis diagnoses in under an hour. The device, called the lab-in-tube assay (LIT), can detect Mycobacterium tuberculosis DNA in saliva, blood, and sputum samples, offering a cost-effective tool for improving TB diagnoses in resource-limited areas.
Researchers at KAIST discovered that DDX54 is the master regulator hindering immunotherapy's effectiveness in lung cancer. Supressing DDX54 enhances immune cell infiltration into tumors and improves immunotherapy efficacy.
Researchers have discovered a protective cloaking mechanism in jumbo phages that shield their genetic material from the host's immune system. This innovation could lead to new therapies for antibiotic-resistant infections.
Researchers developed a CRISPR-based diagnostic test that rapidly detects low levels of pathogen genetic material in blood without nucleic acid amplification. The test demonstrated unprecedented sensitivity and could be used to develop highly sensitive CRISPR-based diagnostic tests for detecting pathogens in minutes.
Researchers have discovered new CRISPR-Cas systems with improved efficiency and specificity, including one from dairy cow bacteria that can target specific gene sequences. The new systems have potential applications in human health, biotechnology and environmental fields.
A breakthrough CRISPR-based test has been developed to diagnose Pneumocystis jirovecii pneumonia (PJP), a life-threatening fungal infection. The test detects RNA from live fungi in blood samples and throat swabs, providing a faster and more accurate diagnosis than current invasive bronchoscopy procedures.
A new method for detecting antibiotic resistance genes (ARGs) in wastewater has been developed by researchers, which uses CRISPR-Cas9 technology to enrich ARG fragments and increase detection sensitivity. This enhanced method was found to detect 1189 more ARGs and 61 more ARG families compared to standard metagenomics methods.
Researchers use CRISPR-Cas9 to remove duplicate chromosomes in trisomy 21 cells, restoring gene expression and cellular phenotypes. The technique shows promise as a potential medical intervention for people with Down syndrome.
Researchers have discovered a new mechanism by which bacteria defend against CRISPR-Cas systems, and how phages counter these defenses. This discovery holds potential to enhance the safety and precision of CRISPR-based technologies.
Researchers discovered 47,350 active putative enhancers associated with Parkinson's disease, schizophrenia, and other neurological disorders. These enhancers were found to regulate gene expression during neuronal differentiation.
Scientists at Duke University have discovered a master epigenetic switch that can be activated using CRISPR to compensate for missing genes in Prader-Willi syndrome. This approach could potentially treat the disease by turning on naturally suppressed genes from one parent, addressing the underlying genetic defect.
Researchers used CRISPR/Cas9 to study the gene function of adenine phosphoribosyl transferase in beans. They found two functional mutants with distinct roles: one affects adenine recycling and the other regulates cytokinins, essential for root growth and nodules.
Researchers used CRISPR/Cas to generate controlled chromosomal inversions in Arabidopsis thaliana, finding no significant changes in epigenetic marks or gene expression. Only minor genome-wide effects were observed, indicating the robustness of the epigenome and transcriptome.
Researchers from the University of Lausanne used genome editing to repair a deleterious domestication mutation in the tomato genome. This resulted in an earlier yielding variety, which could have implications for agriculture and sustainability. The study demonstrates the potential benefits of genome editing for crop breeding.
Researchers at Gladstone Institutes and UCSF identified MED12 as a crucial switch that regulates T cell rest and activation. The study found that MED12 promotes rest in resting cells and activation in activated cells, and its removal led to blurred lines between rest and activation.
Researchers have discovered a major setback in the use of AZD7648 to promote precise gene editing, which causes massive genetic changes and genome instability. Despite this, scientists remain optimistic about advancing CRISPR-Cas technology to treat diseases.
Researchers at the University of Tokyo have developed a new CRISPR-based system to label small extracellular vesicles (sEVs) with RNA barcodes, enabling comprehensive analysis of their biogenesis and release regulators. This system allows for the simultaneous study of thousands of genes and estimation of sEV release from host cells.
Researchers have developed a new CRISPR-Cas method to decipher the function of genetic variants that contribute to cancer. The approach creates tens of thousands of cells with different gene variants, allowing scientists to identify which variants make cancer cells resistant to standard drugs.
A new study uses CRISPR-Cas13 to identify nearly 800 noncoding RNAs that are functional and essential for cell function, including in cancer and human development. The researchers found that these RNA molecules modulate key pathways for cell proliferation and can serve as potential biomarkers and therapeutic targets for cancer treatment.
Researchers have discovered a new type of CRISPR chemistry that floods infected cells with toxic molecules and shuts down activity, preventing viruses from spreading. The discovery sheds light on the complex mechanisms of CRISPR systems and their potential applications as diagnostic tools for infection.
A CRISPR-Cas9 based gene editing therapy has been shown to reduce angioedema attacks and sustainably lower kallikrein levels in patients with hereditary angioedema, offering a potential treatment option. The therapy was tested on 27 patients across two dosages compared to placebo.
A team of researchers has developed strategies to identify regulators of intestinal hormone secretion, which could lead to new treatments for metabolic and gut motility disorders. They used human organoids to study the function of 'nutrient sensors' on hormone-producing cells in the gut.
Researchers at ChristianaCare Gene Editing Institute use CRISPR tools to safely disable gene mutation linked to treatment-resistant melanoma. The approach targets melanoma tumor cells while leaving healthy cells alone, restoring sensitivity to anticancer drugs.
Scientists at St. Jude Children's Research Hospital studied the structure of Fanzor2, a eukaryotic genome-editing protein, to understand its potential for gene editing. The findings reveal that Fanzor2 has a unique RNA-guided nuclease system, which could be harnessed to create more functional and smaller proteins.
The CRISPR-Cas13 system enables temporary gene expression manipulation without permanent genomic changes, holding promise for treating diseases caused by RNA defects. It has been applied to correct mutations linked to Duchenne muscular dystrophy and can be used to alter splicing events, making it a powerful tool in personalized medicine.
Researchers developed a compact 'gene scissor' tool, TnpB, which shows a 4.4-fold increase in efficiency of modifying DNA, making it more effective as a gene editing tool. The tool can be used to treat patients with familial hypercholesterolemia, reducing cholesterol levels by nearly 80%.
CREME, a new AI-powered virtual laboratory, allows scientists to run thousands of virtual experiments with the click of a button to identify key regions of the genome. This breakthrough may lead to discovering new therapeutic targets and giving scientists access to cutting-edge technology without a real laboratory.
Researchers at Osaka Metropolitan University used CRISPR/Cas9 to create a strain of Euglena that produces wax esters with shorter carbon chains, improving their cold flow and suitability as a biofuel feedstock. This breakthrough could potentially replace petroleum-based production of wax esters with biological sources.
A new method called VitelloTag has been developed at the Marine Biological Laboratory, allowing researchers to deliver miniature research tools into egg cells and embryos. The approach uses a yolk protein found in most animals to bind to the receptor on the egg cell surface, enabling efficient delivery of CRISPR-Cas9.
A team of international researchers has discovered a surprising genetic mechanism that influences the vibrant patterns on butterfly wings. An RNA molecule controls where dark pigments are made during butterfly metamorphosis, shaping the butterfly's color patterns in a way previously unforeseen.
Researchers at Montana State University have published a study in Nature describing the discovery of the PARIS immune system, which uses tRNA to neutralize viral infections. The team used advanced microscopy techniques to visualize the system's structure and function.
Michigan State University researchers have developed a method for CRISPR-based genome editing in Nile grass rats, which are diurnal rodents with sleep patterns similar to humans. This breakthrough could provide an alternative model for studying human health and disease, as existing models rely heavily on laboratory mice.
The CRISPR/Cas9 system has been adapted for genome visualization, enabling the study of chromatin dynamics and genome organization in living cells. Recent advancements have expanded its applications to live cell imaging, providing a robust tool for visualizing genomic loci.
A team of scientists has found that the same ancient gene family is responsible for prickles in multiple plants, including roses and eggplants. This discovery sheds light on convergent evolution and could have implications for understanding how similar traits emerge in different species.
A new CRISPR method, SEED/Harvest, has been developed to precisely modify DNA in fruit flies using the Single-Strand Annealing repair pathway. This allows for genome-wide changes with minimal unwanted scars.
Researchers have used CRISPR/Cas9 gene editing to improve groundcherry's growth habit and fruit characteristics. This breakthrough could lead to increased crop yields and reduce the need for pesticides. The study also highlights the potential of groundcherry as a model species for studying plant biology.
A team of scientists at the University of Sydney has repurposed a commonly used blood thinner, heparin, as an inexpensive antidote for cobra venom. The discovery could drastically reduce the impact of snakebites worldwide, particularly in low- and middle-income countries where cobra species account for most snakebite incidents.
Researchers have developed a new method called PUMA that uses CRISPR-Cas12 nucleases to detect RNA biomarkers. This technology overcomes limitations of existing methods by reprogramming tracrRNAs to recognize specific RNA targets, enabling precise detection without requiring a specific recognition sequence.
A team of researchers led by Professor Peter Fineran from the University of Otago discovered a novel regulatory mechanism in a protein used by phages to deploy anti-CRISPR. This finding has significant implications for understanding gene regulation and developing new antimicrobial therapies.
A team of scientists at Gladstone Institutes has developed a new method that enables them to make precise edits in multiple locations within a cell—all at once. They created a tool using molecules called retrons to efficiently modify DNA in bacteria, yeast, and human cells.