Articles tagged with Crisprs
Researchers at Rice University have developed a non-invasive gene delivery technique using ultrasound to efficiently deliver clinically used gene therapy vectors throughout the brain. The study, published in Gene Therapy, shows that opening more sites within targeted regions improves gene delivery efficiency.
Researchers create adenine base editor with 'on/off' switch, reducing off-target edits by over 70% and increasing accuracy of on-target edits. The tool has potential to correct nearly half of disease-causing point mutations in human genome.
Researchers at ETH Zurich have created a method to simultaneously modify multiple genes in individual cells of an animal, allowing for the study of complex diseases. By precisely analyzing these cells, scientists can identify key genes responsible for disease progression, paving the way for potential drug development.
A new CRISPR-based diagnostic tool, MPXV-CRISPR, has been developed in Australia to detect the monkeypox virus with high precision and speed. The tool can detect the virus in clinical samples in just 45 minutes, making it faster than current methods.
Researchers at Osaka University have developed a new gene editing technique called NICER, which significantly reduces off-target mutations compared to traditional CRISPR/Cas9 methods. This novel approach uses multiple small cuts in DNA strands and promotes interhomolog homologous recombination to correct heterozygous mutations.
Researchers developed a technology to rapidly screen genetic edits in immune cells, identifying a new combination that improves their effectiveness against cancers. By combining multiple genes into long DNA stretches and testing thousands of combinations, scientists discovered that different CARs can be optimized by different factors.
Researchers analyzed Wikipedia's CRISPR-related articles to understand the site's role in documenting scientific history. The study found that Wikipedia's content reflects global conversations on cutting-edge technologies like CRISPR.
Researchers at Gladstone Institutes identified conditions that enable gamma delta T cells to recognize cancer cells by disrupting energy production and causing cellular stress. This insight suggests that therapies manipulating butyrophilin abundance on the surface of cancer cells could boost gamma delta T cell effectiveness.
CyDENT base editors allow efficient and precise modification of genetic information in living organisms. The system enables strand-specific base editing in nuclear and organellar genomes, with high strand specificity demonstrated in mitochondrial genome editing.
A CRISPR-Cas3 system has restored dystrophin protein function in induced pluripotent stem cells from patients with Duchenne muscular dystrophy. The approach uses a dual CRISPR RNA method to remove large sections of the dystrophin gene, yielding truncated but still functional proteins for various mutation patterns.
Researchers used base editors to introduce specific combinations of activating and inactivating mutations into healthy organoids, creating realistic models for various types of cancer. This allows for further investigation into the development and treatment of cancer, with potential applications including testing new drugs.
A novel CRISPR-based gene-editing treatment, EBT-001, effectively removes SIV from the genomes of non-human primates without off-target effects. The study's findings support the development of a cure for HIV/AIDS in humans and pave the way for ongoing clinical trials.
Researchers have engineered bacteria that can detect tumor DNA in a live organism, using CRISPR technology. The bacteria, Acinetobacter baylyi, were designed to respond to specific DNA sequences associated with cancer, allowing for early detection and potentially preventing disease progression.
Researchers at the Marine Biological Laboratory have devised a method to precisely alter rotifer genomes using CRISPR-Cas9, enabling the study of fundamental biology and evolution. The new approach will allow scientists to investigate various aspects of biology, including aging, DNA repair mechanisms, and mitochondrial function.
Researchers at A*STAR and NUS Medicine developed a CRISPR-Cas13 therapeutic that directly targets and eliminates EV-A71 RNA viruses in laboratory models. The treatment shows potent reduction of viral burden, clearing infection and preventing organ damage and mortality.
Researchers successfully modified the ethylene synthesis pathway in the Japanese luxury melon to increase its shelf-life. The study found that introducing a mutation into the CmACO1 gene reduced ethylene generation, resulting in firmer fruit and longer shelf life.
Researchers at Cornell University have discovered a mutation in the MdLAZY1A gene responsible for the 'weeping' growth pattern in apple trees. This finding could lead to more productive and labor-saving orchards by allowing branches to grow downwards, thereby increasing resource allocation towards reproductive growth.
Gang Bao's lab receives a 4-year, $2.6 million grant from the National Institutes of Health to investigate the safety and efficacy of using gene editing treatments like CRISPR-Cas9 to treat sickle cell disease. The team aims to understand the mechanisms behind large gene modifications and their biological consequences.
Hematopoietic stem cell culture technology improves genome editing in HSCs by increasing successful correction rates to 100%, eliminating genetic mutations, and enhancing cell transplantation outcomes. This breakthrough enhances the efficiency and safety of gene editing in treating genetic diseases.
Scientists have developed a new genetic technology called Ifegenia that suppresses populations of Anopheles gambiae mosquitoes, which primarily spread malaria in Africa. The system targets females, which are the primary disease carriers, and kills them, halting parasite transmission.
A new study from Aarhus University has found that applying AI predictions of protein structures enhances the CRISPR technology, making the cuts in a patient's DNA more precise. This discovery may lead to better treatments for patients with genetic disorders and potentially develop cures for various genetic diseases.
Researchers have discovered that gene editing technologies may introduce unintended mutations and damage to DNA in early human embryos. The study found that most cells repair breaks in the DNA using non-homologous end joining, which can lead to additional genetic abnormalities.
Researchers used a stem cell model to study the effects of Alzheimer's disease-associated mutations on early human brain development, finding that mutant spheres were larger and contained fewer mature neurons. The study highlights the need for tailored therapies and paves the way for studying Alzheimer's in its early stages.
A study reports a CRISPR/Cas9 delivery system that reduces anxiety-related behavior in mice by modulating neuronal receptor pathways. The treatment, delivered through the nose, shows a 35.7% increase in time spent in light areas and a 14.8% decrease in marble burial compared to controls.
Researchers successfully engineered an albino strain of the hummingbird bobtail squid, offering clear optical access for visualizing its nervous system. This breakthrough presents Euprymna berryi as a viable candidate for a model cephalopod, enabling scientists to study complex animal behavior and unlock secrets of biology.
A team of scientists used CRISPR-Cas genome editing to create a disease-resistant rice variety, which showed high yields and resistance to the fungus that causes rice blast. The new rice variety produced five times more yield than the control rice in small-scale field trials.
Researchers developed a CRISPR/Cas9-based gene drive system targeting the Drosophila suzukii doublesex gene, resulting in female sterility and high transmission rates. Mathematical modeling predicts efficient population suppression with low release ratios.
Researchers at Osaka University developed a highly reproducible genetic screen to investigate Toxoplasma's survival within hosts. The study identified IFN-γ-dependent and -independent virulence factors that promote parasite fitness, providing potential targets for treatment and prevention of toxoplasmosis.
A new study using CRISPR/Cas9 technology has identified a critical gene, SLC4γ, required for young coral colonies to build their skeletons. This gene is unique to stony corals and may have evolved to support skeleton formation.
Scientists at Temple University have developed a novel gene-editing strategy that disrupts the ability of HIV-1 virus to enter host cells by targeting a rare genetic disorder. This approach may offer another target for developing next-generation CRISPR technology for HIV elimination, while avoiding adverse effects on cell mortality.
Researchers developed a biobank of head and neck cancer organoids to validate biomarkers and predict treatment responses. The study found that organoid responses matched patient outcomes, suggesting potential for personalized therapies.
Scientists have developed a gene-editing technique that allows them to easily engineer specific cancer-linked mutations into mouse models. This new method, based on CRISPR genome-editing technology, enables researchers to explore many unknown mutations and develop new drugs targeting those mutations.
Scientists from Forsyth Institute develop CRISPR-based diagnostic tool that can detect specific oral pathogens with high sensitivity and specificity. The test can be performed in a dental office without technical expertise, providing comprehensive information on oral health and systemic diseases like diabetes, heart disease, and cancer.
Scientists have developed a method to increase the efficiency of CRISPR/Cas9 gene editing without viral material, stimulating homology-directed repair by threefold. This breakthrough improves nonviral gene editing and may lead to more efficient disease modeling and hypothesis testing.
Researchers have engineered a new CRISPR-based drug candidate targeting E. coli directly while preserving the microbiome. The innovative treatment has shown promise in reducing E. coli burden in mice and is now in phase 1 clinical trials to treat blood cancer patients and prevent deadly infections.
A gene-edited calf demonstrates reduced susceptibility to bovine viral diarrhea virus (BVDV) after intentionally altering the CD46 receptor with CRISPR/Cas9. The healthy calf showed no measurable infectious virus in its blood, despite both calves developing antibodies to the virus.
Researchers used human liver organoids to study fibrolamellar carcinoma, a rare childhood liver cancer. They found that different genetic mutations underlie different degrees of aggressiveness in the tumors, and uncovered the probable cell-of-origin as hepatocytes.
Researchers developed a new approach to genetic engineering of cells, promising improvements in speed and efficiency over current methods. The technique uses special cell-penetrating peptides to deliver CRISPR-Cas molecules into cells with up to 100% efficiency and low toxicity.
A new study reveals that a Cas protein and a membrane protein work together to enhance anti-viral defense in bacteria. The team found that the membrane protein forms a pore-like structure that disrupts energy production and hinders virus replication, effectively 'pulling the plug' on viral infections.
A University of Colorado at Boulder research team has discovered a protein crucial for repairing DNA in cancer cells, which they found can be selectively targeted to kill cancer cells without harming healthy ones.
The funding will support the development of next-generation delivery technology for mRNA vaccines and CRISPR-based genome editing. This will enable broader application of messenger RNA therapeutics, including for various diseases and immunological properties of nanoparticles.
Researchers generated a POLDIP2 knockout ARPE-19 cell line and found reduced mitochondrial superoxide levels, consistent with upregulated SOD2. The study demonstrates a potential role of POLDIP2 in regulating oxidative stress in AMD.
Researchers developed an optimized genome-editing method that vastly reduces mutations, enabling more effective treatment of genetic diseases. The new technique uses a 'safeguard gRNA' to control DNA cleavage, reducing off-target effects and cytotoxicity.
Researchers at the University of Tokyo have discovered the 3D structure of TnpB, a protein involved in genome editing and a probable precursor to the CRISPR-Cas12 enzyme. The study reveals how TnpB recognizes and cuts DNA using a unique pseudoknot shape similar to that found in guide RNAs of Cas12 enzymes.
Scientists at the University of Colorado Anschutz Medical Campus identified protein FAM193A as a key component of a mechanism suppressing tumor growth. The discovery offers new potential for cancer therapies targeting p53, a frequently mutated gene in human cancers.
A recent study identified novel genes that influence PARP inhibitor response in prostate cancer, including MMS22L and RNASEH2B. The research found that loss of CHEK2, a previously approved biomarker, confers resistance to PARP inhibition, highlighting the need for comprehensive genomic analysis to improve treatment decisions.
Genetic welding proposes using CRISPR-Cas9 technology to rapidly change evolutionary courses in animals or plants. Cutting argues that this method requires scientific and ethical scrutiny before its application.
Researchers developed O-ClickFC technology to analyze lipid metabolic state in cells at high speed. The technology combined with genome editing enabled identification of causative genes of metabolic disorders, including FLVCR1 and its role in choline uptake.
Researchers have found that valosin-containing protein (VCP) is essential for KRAS-mutant pancreatic ductal adenocarcinoma cell growth and survival. Inhibiting VCP, combined with autophagy inhibition, enhances efficacy in preclinical studies.
Researchers found that the Alba morph in female Colias butterflies evolved once near the last common ancestor over 1.2 million generations ago. The genetic basis of Alba was identified as a regulatory region in DNA, maintained through gene flow and balancing selection.
Researchers have successfully restored vision in mice with retinitis pigmentosa using a new CRISPR-based genome editing technique. The PE SpRY system corrected genetic mutations and restored normal electrical responses to light, preserving vision into old age. This breakthrough offers potential for treating inherited blindness.
A team of researchers has developed a sensitive method to detect viral nucleic acids using 'glow-in-the-dark' proteins, achieving high sensitivity and speed for clinical diagnostic tests. The LUNAS assay successfully detected SARS-CoV-2 RNA in under 20 minutes at low concentrations.
Researchers from the Wellcome Sanger Institute have established a system to report CRISPR activation effectiveness in stem cells, revealing key features influencing its efficiency. The study found that bivalent genes can be robustly activated by CRISPRa and that cell state and gene location impact its success.
Researchers have developed a new method for downregulating gene translation in plants using upstream open reading frames (uORFs). The study, published in Nature Biotechnology, demonstrates the potential for precise and incremental regulation of gene expression.
Researchers identified a new mutation in the desmoplakin gene that leads to cardiac disease arrhythmogenic cardiomyopathy (ACM). The mutation affects heart muscle cell connections and ion channel function, highlighting the importance of desmosomes in maintaining healthy heart function.
A review of gene editing techniques suggests that the CRISPR/Cas method could be a game-changer for rice crops threatened by climate change and high food demand. The study highlights its efficiency in improving yield, tolerance to biotic and abiotic stresses, and grain quality.
Gene therapy using CRISPR-Cas9 lipid nanoparticles has been shown to be highly effective in reducing target protein expression in mice. The new delivery system increases the efficiency of in vivo gene therapy, paving the way for safe and effective treatment.
Researchers created human organoid models of fatty liver disease to shed light on drug responses and disease biology. The models identified a common mechanism for effective drugs that block lipid generation from sugars, suggesting personalized medicine applications.
A recent study published in the Journal of Clinical Investigation found that PAX5 is strongly associated with impaired insulin secretion in type 2 diabetes. The research team identified 94 previously known genes and discovered many new genes differently expressed in individuals with type 2 diabetes.
Researchers discovered a cluster of enzymes in bacteria that can be reprogrammed to edit proteins and potentially treat human diseases such as Parkinson's and Crohn's. The study also identified key components of the bacterial immune system, including two on-off switches, that could be targeted therapeutically.