Articles tagged with Crisprs
Comprehensive exploration of living organisms, biological systems, and life processes across all scales from molecules to ecosystems. Encompasses cutting-edge research in biology, genetics, molecular biology, ecology, biochemistry, microbiology, botany, zoology, evolutionary biology, genomics, and biotechnology. Investigates cellular mechanisms, organism development, genetic inheritance, biodiversity conservation, metabolic processes, protein synthesis, DNA sequencing, CRISPR gene editing, stem cell research, and the fundamental principles governing all forms of life on Earth.
Comprehensive medical research, clinical studies, and healthcare sciences focused on disease prevention, diagnosis, and treatment. Encompasses clinical medicine, public health, pharmacology, epidemiology, medical specialties, disease mechanisms, therapeutic interventions, healthcare innovation, precision medicine, telemedicine, medical devices, drug development, clinical trials, patient care, mental health, nutrition science, health policy, and the application of medical science to improve human health, wellbeing, and quality of life across diverse populations.
Comprehensive investigation of human society, behavior, relationships, and social structures through systematic research and analysis. Encompasses psychology, sociology, anthropology, economics, political science, linguistics, education, demography, communications, and social research methodologies. Examines human cognition, social interactions, cultural phenomena, economic systems, political institutions, language and communication, educational processes, population dynamics, and the complex social, cultural, economic, and political forces shaping human societies, communities, and civilizations throughout history and across the contemporary world.
Fundamental study of the non-living natural world, matter, energy, and physical phenomena governing the universe. Encompasses physics, chemistry, earth sciences, atmospheric sciences, oceanography, materials science, and the investigation of physical laws, chemical reactions, geological processes, climate systems, and planetary dynamics. Explores everything from subatomic particles and quantum mechanics to planetary systems and cosmic phenomena, including energy transformations, molecular interactions, elemental properties, weather patterns, tectonic activity, and the fundamental forces and principles underlying the physical nature of reality.
Practical application of scientific knowledge and engineering principles to solve real-world problems and develop innovative technologies. Encompasses all engineering disciplines, technology development, computer science, artificial intelligence, environmental sciences, agriculture, materials applications, energy systems, and industrial innovation. Bridges theoretical research with tangible solutions for infrastructure, manufacturing, computing, communications, transportation, construction, sustainable development, and emerging technologies that advance human capabilities, improve quality of life, and address societal challenges through scientific innovation and technological progress.
Study of the practice, culture, infrastructure, and social dimensions of science itself. Addresses how science is conducted, organized, communicated, and integrated into society. Encompasses research funding mechanisms, scientific publishing systems, peer review processes, academic ethics, science policy, research institutions, scientific collaboration networks, science education, career development, research programs, scientific methods, science communication, and the sociology of scientific discovery. Examines the human, institutional, and cultural aspects of scientific enterprise, knowledge production, and the translation of research into societal benefit.
Comprehensive study of the universe beyond Earth, encompassing celestial objects, cosmic phenomena, and space exploration. Includes astronomy, astrophysics, planetary science, cosmology, space physics, astrobiology, and space technology. Investigates stars, galaxies, planets, moons, asteroids, comets, black holes, nebulae, exoplanets, dark matter, dark energy, cosmic microwave background, stellar evolution, planetary formation, space weather, solar system dynamics, the search for extraterrestrial life, and humanity's efforts to explore, understand, and unlock the mysteries of the cosmos through observation, theory, and space missions.
Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
A new study using CRISPR/Cas9 technology has identified a critical gene, SLC4γ, required for young coral colonies to build their skeletons. This gene is unique to stony corals and may have evolved to support skeleton formation.
Scientists at Temple University have developed a novel gene-editing strategy that disrupts the ability of HIV-1 virus to enter host cells by targeting a rare genetic disorder. This approach may offer another target for developing next-generation CRISPR technology for HIV elimination, while avoiding adverse effects on cell mortality.
Researchers developed a biobank of head and neck cancer organoids to validate biomarkers and predict treatment responses. The study found that organoid responses matched patient outcomes, suggesting potential for personalized therapies.
Scientists from Forsyth Institute develop CRISPR-based diagnostic tool that can detect specific oral pathogens with high sensitivity and specificity. The test can be performed in a dental office without technical expertise, providing comprehensive information on oral health and systemic diseases like diabetes, heart disease, and cancer.
Scientists have developed a method to increase the efficiency of CRISPR/Cas9 gene editing without viral material, stimulating homology-directed repair by threefold. This breakthrough improves nonviral gene editing and may lead to more efficient disease modeling and hypothesis testing.
Scientists have developed a gene-editing technique that allows them to easily engineer specific cancer-linked mutations into mouse models. This new method, based on CRISPR genome-editing technology, enables researchers to explore many unknown mutations and develop new drugs targeting those mutations.
A gene-edited calf demonstrates reduced susceptibility to bovine viral diarrhea virus (BVDV) after intentionally altering the CD46 receptor with CRISPR/Cas9. The healthy calf showed no measurable infectious virus in its blood, despite both calves developing antibodies to the virus.
Researchers have engineered a new CRISPR-based drug candidate targeting E. coli directly while preserving the microbiome. The innovative treatment has shown promise in reducing E. coli burden in mice and is now in phase 1 clinical trials to treat blood cancer patients and prevent deadly infections.
Researchers used human liver organoids to study fibrolamellar carcinoma, a rare childhood liver cancer. They found that different genetic mutations underlie different degrees of aggressiveness in the tumors, and uncovered the probable cell-of-origin as hepatocytes.
Researchers developed a new approach to genetic engineering of cells, promising improvements in speed and efficiency over current methods. The technique uses special cell-penetrating peptides to deliver CRISPR-Cas molecules into cells with up to 100% efficiency and low toxicity.
A new study reveals that a Cas protein and a membrane protein work together to enhance anti-viral defense in bacteria. The team found that the membrane protein forms a pore-like structure that disrupts energy production and hinders virus replication, effectively 'pulling the plug' on viral infections.
A University of Colorado at Boulder research team has discovered a protein crucial for repairing DNA in cancer cells, which they found can be selectively targeted to kill cancer cells without harming healthy ones.
The funding will support the development of next-generation delivery technology for mRNA vaccines and CRISPR-based genome editing. This will enable broader application of messenger RNA therapeutics, including for various diseases and immunological properties of nanoparticles.
Researchers generated a POLDIP2 knockout ARPE-19 cell line and found reduced mitochondrial superoxide levels, consistent with upregulated SOD2. The study demonstrates a potential role of POLDIP2 in regulating oxidative stress in AMD.
Researchers developed an optimized genome-editing method that vastly reduces mutations, enabling more effective treatment of genetic diseases. The new technique uses a 'safeguard gRNA' to control DNA cleavage, reducing off-target effects and cytotoxicity.
Researchers at the University of Tokyo have discovered the 3D structure of TnpB, a protein involved in genome editing and a probable precursor to the CRISPR-Cas12 enzyme. The study reveals how TnpB recognizes and cuts DNA using a unique pseudoknot shape similar to that found in guide RNAs of Cas12 enzymes.
Scientists at the University of Colorado Anschutz Medical Campus identified protein FAM193A as a key component of a mechanism suppressing tumor growth. The discovery offers new potential for cancer therapies targeting p53, a frequently mutated gene in human cancers.
A recent study identified novel genes that influence PARP inhibitor response in prostate cancer, including MMS22L and RNASEH2B. The research found that loss of CHEK2, a previously approved biomarker, confers resistance to PARP inhibition, highlighting the need for comprehensive genomic analysis to improve treatment decisions.
Genetic welding proposes using CRISPR-Cas9 technology to rapidly change evolutionary courses in animals or plants. Cutting argues that this method requires scientific and ethical scrutiny before its application.
Researchers developed O-ClickFC technology to analyze lipid metabolic state in cells at high speed. The technology combined with genome editing enabled identification of causative genes of metabolic disorders, including FLVCR1 and its role in choline uptake.
Researchers have found that valosin-containing protein (VCP) is essential for KRAS-mutant pancreatic ductal adenocarcinoma cell growth and survival. Inhibiting VCP, combined with autophagy inhibition, enhances efficacy in preclinical studies.
Researchers found that the Alba morph in female Colias butterflies evolved once near the last common ancestor over 1.2 million generations ago. The genetic basis of Alba was identified as a regulatory region in DNA, maintained through gene flow and balancing selection.
Researchers have successfully restored vision in mice with retinitis pigmentosa using a new CRISPR-based genome editing technique. The PE SpRY system corrected genetic mutations and restored normal electrical responses to light, preserving vision into old age. This breakthrough offers potential for treating inherited blindness.
A team of researchers has developed a sensitive method to detect viral nucleic acids using 'glow-in-the-dark' proteins, achieving high sensitivity and speed for clinical diagnostic tests. The LUNAS assay successfully detected SARS-CoV-2 RNA in under 20 minutes at low concentrations.
Researchers from the Wellcome Sanger Institute have established a system to report CRISPR activation effectiveness in stem cells, revealing key features influencing its efficiency. The study found that bivalent genes can be robustly activated by CRISPRa and that cell state and gene location impact its success.
Researchers have developed a new method for downregulating gene translation in plants using upstream open reading frames (uORFs). The study, published in Nature Biotechnology, demonstrates the potential for precise and incremental regulation of gene expression.
A review of gene editing techniques suggests that the CRISPR/Cas method could be a game-changer for rice crops threatened by climate change and high food demand. The study highlights its efficiency in improving yield, tolerance to biotic and abiotic stresses, and grain quality.
Researchers identified a new mutation in the desmoplakin gene that leads to cardiac disease arrhythmogenic cardiomyopathy (ACM). The mutation affects heart muscle cell connections and ion channel function, highlighting the importance of desmosomes in maintaining healthy heart function.
Gene therapy using CRISPR-Cas9 lipid nanoparticles has been shown to be highly effective in reducing target protein expression in mice. The new delivery system increases the efficiency of in vivo gene therapy, paving the way for safe and effective treatment.
Researchers created human organoid models of fatty liver disease to shed light on drug responses and disease biology. The models identified a common mechanism for effective drugs that block lipid generation from sugars, suggesting personalized medicine applications.
A recent study published in the Journal of Clinical Investigation found that PAX5 is strongly associated with impaired insulin secretion in type 2 diabetes. The research team identified 94 previously known genes and discovered many new genes differently expressed in individuals with type 2 diabetes.
Researchers discovered a cluster of enzymes in bacteria that can be reprogrammed to edit proteins and potentially treat human diseases such as Parkinson's and Crohn's. The study also identified key components of the bacterial immune system, including two on-off switches, that could be targeted therapeutically.
Researchers developed an AI algorithm to predict prime editing efficiency and accuracy, enabling faster and more precise genome editing. By analyzing a large data set of over 100,000 pegRNAs, the tool identifies key properties influencing prime editing success.
A new study uses CRISPR-Cas9 to modify a gene that makes plants susceptible to viruses, resulting in strong resistance to multiple potyvirus isolates. This approach broadens genetic diversity and generates resistance without altering protein function or expression.
A study by UC San Francisco and Stanford Medicine found that removing the oxytocin receptor does not interfere with monogamy or giving birth. Prairie voles bred without receptors for oxytocin showed similar mating, attachment, and parenting behaviors as regular voles.
Researchers from Heidelberg University have developed a new 'VIP admission ticket' that enables efficient delivery of enzymes to the nucleus, enhancing the efficiency of CRISPR/Cas9 and related methods. This breakthrough opens up new areas for genetic screening and potentially therapeutic applications.
The CABBI team successfully demonstrated precision gene editing in miscanthus, a promising perennial crop for sustainable bioenergy production. The results will accelerate efforts to tap the huge potential of this highly productive but genetically complex grass as a source for biofuels, renewable bioproducts, and carbon sequestration.
Researchers at Massachusetts General Hospital created a new class of technologies called CRISPR-associated transposases (CASTs) to overcome diverse disease-causing mutations. The optimized approach improves product purity and genome-wide specificity, offering a potential solution for inserting entire genes into the genome.
Researchers developed a flexible genetic hacking system to convert split gene drives into full gene drives, enabling safe testing and potential real-world applications. The new system revealed surprising fitness costs of full drive systems, with slower-than-predicted spread rates in cage experiments.
Scientists have identified a unique CRISPR nuclease that not only recognizes and cleaves viral RNA but also damages other RNA and DNA inside the cell, impairing bacterial growth. This discovery opens up new possibilities for molecular diagnostics and direct detection of RNA biomarkers.
Researchers at Utah State University have discovered a newly found CRISPR immune system, Cas12a2, which deactivates foreign genes to protect cells. This unique system elicits abortive infection in target cells, causing them to become senescent.
Researchers discovered a new CRISPR protein that can degrade single-stranded RNA, DNA, and double-stranded DNA, enabling the development of inexpensive and highly sensitive diagnostic tests for various infectious diseases. The test could combine high sensitivity and accuracy with rapid at-home diagnostic features.
The new PASTE tool combines precise targeting of CRISPR-Cas9 with integrases to insert large chunks of DNA into the genome without inducing double-stranded breaks. This approach holds promise for treating diseases with multiple mutations, such as cystic fibrosis, with high efficiency and minimal unwanted effects.
A new microfluidic multiplexed chip uses CRISPR technology to detect SARS-CoV-2 and monitor antibiotic levels, offering a rapid and sensitive solution for managing COVID-19 patients. The test omits nucleic acid amplification and can be easily adapted to new virus mutations.
Researchers at the University Hospital Bonn have discovered a new function of CRISPR/Cas9 gene scissors, which produce small signal molecules that bind to proteins, activating an emergency response. This discovery opens up new possibilities for treating diseases using CRISPR technology.
Scientists have discovered that the ferredoxin/thioredoxin pathway is essential for light-dependent activation reactions in chloroplasts, crucial for normal plant growth and efficient photosynthesis. The study used CRISPR/Cas9 technology to create a mutated plant specimen with a defective Fd/Trx pathway.
Researchers develop CRISPR-Cas systems associated with transposons to rewrite large chunks of DNA in organisms like E. coli. This expands the CRISPR toolbox for flexible genome editing and has significant implications for therapeutics, biotechnology, and agriculture.
A new CRISPR genome editing treatment has shown promising results in alleviating swelling and reducing the frequency of attacks in hereditary angioedema patients. The treatment, NTLA-2002, targets the KLKB1 gene and reduces kallikrein production, a protein responsible for debilitating swelling attacks.
Researchers at Sloan Kettering Institute develop MACHETE, a novel technique to study copy number alterations in cancer genomes. The method enables the efficient study of CNA deletions in laboratory models, which may help identify patients likely to respond to immunotherapies.
New insights into the processing of hormones in the human gut reveal dozens of peptides regulating appetite, bowel movement, and insulin secretion. By studying human intestinal organoids, researchers characterized potentially novel gut hormones, including glucagon, and explored its role in human physiology.
Researchers at North Carolina State University developed a CRISPR-based system that uses engineered bacteriophages to deliver genetic payloads to specific bacteria, even in complex environments. This technology enables precise single-letter changes to the genome without double-strand DNA breakage.
Researchers discover chemical inhibitor TIS108 significantly lowers Striga infestation without affecting plant growth or grain yield. The study shows canonical strigolactones contribute to seed germination in root parasitic weeds and play a major role in stimulating invasion by Striga.
Researchers at MIT have developed a new control system for synthetic genes that can precisely regulate protein production in mammalian cells. The system uses CRISPR proteins to activate target genes and can be tuned to produce specific quantities of proteins, such as monoclonal antibodies.
Researchers at Kyoto University have developed a prodrug form of curcumin called TBP1901, which has shown anti-tumor effects without causing harm. The study found that TBP1901's conversion to active curcumin is dependent on the enzyme GUSB, suggesting its potential therapeutic targets.
Researchers have successfully treated six children with relapsed and treatment-resistant B-ALL using CRISPR-edited T cells, achieving deep remission in four patients. The treatment has shown promise as a viable alternative to currently available treatments.
Researchers have developed a pioneering gene editing strategy that can repair faulty genes in immune cells, offering new hope for patients with conditions like CTLA-4 insufficiency. The technique uses CRISPR/Cas9 to target and correct the faulty gene, preserving important regulatory mechanisms.
Researchers at VCU Massey Cancer Center have identified a new therapeutic target for triple-negative breast cancer (TNBC), a more aggressive and deadly form of disease. Using CRISPR/CAS9 screening, scientists pinpointed the UBA1 enzyme as an ideal target, which can be inhibited by the novel drug TAK-243 to effectively kill cancer cells.
Researchers at Rice University have developed a procedure to quantify unintended changes that accompany on-target CRISPR-Cas9 gene editing, potentially threatening the efficacy and safety of therapies. The new method uses single-molecule sequencing with unique molecular identifiers to detect large deletions, insertions, and chromosomal...
A new CRISPR-Cas approach, SpRYgests, allows for precise DNA cleavage at any sequence, expanding the utility of molecular cloning approaches. This innovation simplifies and expedites DNA editing and has potential clinical implications.
The study found that microbial enzymes are essential for the digestion of pectin in leaf beetles, allowing them to access nutrient-rich plant cells. The researchers also discovered that leaf beetle species acquire these enzymes through horizontal gene transfer from other microbes.