Articles tagged with Gene Targeting
A Japanese research group has developed a CRISPR/Cas9 system to target and block HIV-1 production in infected cells. By targeting two regulatory genes tat and rev, they were able to significantly lower the expression and functions of both genes.
Researchers have developed a novel CRISPR-Cas9 technology that enables precise editing of any gene in the yeast Saccharomyces cerevisiae by deleting single nucleotide changes. This allows for individual gene studies and optimization of genome engineering, potentially increasing productivity in industries such as ethanol production.
A study by Dana-Farber Cancer Institute and Broad Institute identified five genetic subtypes of diffuse large B cell lymphoma, which can help predict individual patient outcomes and guide personalized treatment. The analysis revealed clear links between specific genetic signatures and how patients respond to standard treatment.
Scientists at the University of Cambridge have identified N-acetyltransferase 10 (NAT10) as a potential therapeutic target for Hutchinson-Gilford Progeria Syndrome (HGPS), a rare condition that causes premature ageing. In a mouse model, chemical inhibition or genetic deregulation of NAT10 led to significant health and lifespan gains.
A new study led by researchers at the Nara Institute of Science and Technology found that plants use SOG1 to repair DNA damage, but unlike p53 in animals, SOG1 targets genes involved in immune response only to fungal infections.
A new gene-editing technology has been developed to improve the efficiency of CRISPR/Cas9, allowing for safer and more efficient correction of disease-causing mutations in patients. The system uses a nano-sized porous material to coat the molecular components of CRISPR/Cas9, enabling efficient release into cells.
Researchers at Osaka University have developed a novel CRISPR-Cas9 method that can introduce precise modifications to defective genes with fewer safety drawbacks. The new technique, called Single Nicking in the target Gene and Donor (SNGD), reduces unintended genetic mutations by up to 95%.
Smokers with targetable genetic alterations in their lung cancer benefit equally from targeted therapies as non-smokers. Targeted treatments improve survival by 1.5 years, regardless of smoking status.
A new study found that person-to-person genetic differences can impact the efficacy of gene editing technologies like CRISPR-Cas9. The researchers analyzed 7,444 whole-genome sequences and discovered that about 50% of guide RNAs could be affected by variants at their target sites.
Researchers at the University of Edinburgh's Roslin Institute are investigating gene drive technology to curb pest rodent populations. They aim to reduce or eliminate pest species using a more humane method.
Scientists have developed a new CRISPR RNA editing tool called REPAIR, which can target and edit RNA with high efficiency and specificity. This tool allows for the correction of mutations in different time windows, including during key developmental periods, and may have disease-modifying potential.
Insilico Medicine showcases its use of artificial intelligence to identify disease targets, generate molecular structures, and track interventions for metabesity. The company aims to prevent metabolic-rooted disorders such as diabetes and dementia.
The Xpert Ultra assay overcomes limitations of the current test, providing more reliable detection of Rifampicin resistance and significantly improving tuberculosis detection in patients with pauci-bacillary disease. The new assay format includes gene target amplifications that enhance previously amplified targets.
A comprehensive genomic analysis of Wilms tumor identified genetic mutations involving a large number of genes, converging into two major pathways that lead to cancer. The study offers new opportunities for treatment by targeting these processes instead of individual genes.
Researchers at MPFI have developed a novel technique to selectively target cerebellar interneurons, which are crucial for regulating motor behavior and learning. This breakthrough allows scientists to manipulate the activity of these cells, providing new insights into the role of interneurons in cerebellar function.
Scientists have identified how cytokinin activates genes to regulate plant growth, allowing for stem cell establishment in grains like rice and corn. The study reveals the mechanism of type-B ARRs in regulating cytokinin response and growing shoots in tissue culture.
The CRISPR-Cpf1 gene editing system has been improved by incorporating a firefly gene, enabling the simultaneous targeting of multiple genes in human cells. This advance could be useful for treating diseases such as hepatitis B and muscular dystrophy.
Research reveals rosiglitazone treatment increases brown fat genes in mice, reversing genetic alterations associated with obesity. The findings suggest environmental changes can reverse some genetic defects linked to type 2 diabetes.
Scientists have engineered the smallest CRISPR-Cas9 system to date, capable of delivering gene editing to muscles and eyes via AAV. This technology targets age-related macular degeneration (AMD) and other 'undruggable' genes, offering a promising therapeutic tool.
Researchers have successfully produced live cows with increased resistance to bovine tuberculosis using a modified version of CRISPR gene-editing technology. The new method resulted in no off-target effects on the animals' genetics, making it a promising approach for producing transgenic livestock.
Researchers developed a new gene-editing method called homology-independent targeted integration (HITI) that efficiently inserts DNA into genes in dividing and non-dividing cells of living rats. The technique uses the CRISPR-Cas9 tool to cut DNA at a specific location, followed by the NHEJ repair pathway for insertion.
Researchers identified five genes that, when inactivated, protected cells from HIV infection without affecting cellular survival. The genes target human proteins essential for HIV replication and viral entry into CD4 T cells.
Researchers used CRISPR-Cas9 gene-editing technology to identify three promising new targets for HIV infection. The study screened human genes essential for HIV replication but not for cellular survival, and identified five genes with potential therapeutic applications.
Researchers have developed a new enzyme called TevCas9, which cuts DNA in two places instead of one, making it more difficult for DNA-repair to regenerate the site of the cut. This modification shows promise at being more specific in targeting genes and less likely to cause off-target effects.
New research reveals microRNAs play a vital role in regulating gene expression, influencing organismic development and disease. Studies have uncovered a vast network of miRNA families that target disparate gene pathways.
Researchers at Rosalind Franklin University and Oregon Health & Science University developed a breakthrough study on treating congenital diseases in utero using antisense oligonucleotides injected into the amniotic cavity. The procedure resulted in targeted alteration of gene expression for up to a month after birth.
A recent study by FAU researchers found that up to 56% of e-mail recipients and 40% of Facebook users clicked on links from unknown senders, primarily driven by curiosity about the content or sender's identity. The experiment revealed that participants' awareness of security risks was often lower than their actual clicking behavior.
Scientists at the Gladstone Institutes have invented a new way to read and interpret the human genome, using machine learning technology to predict gene-enhancer interactions. The TargetFinder tool accurately predicts complex three-dimensional interactions up to 85% of the time, opening the door to treating genetic diseases.
Researchers at University of California, San Diego School of Medicine successfully targeted RNA in living cells using CRISPR-Cas9. This breakthrough may lead to new therapeutic approaches for diseases linked to defective RNA transport, such as autism and cancer.
Researchers aim to develop precision treatment for HCC by targeting genetic abnormalities and chromosomal alterations. Targeted agents such as sorafenib have been developed, but further research is needed to utilize molecular profiles for therapy selection.
Researchers at Imperial College London have genetically modified Anopheles gambiae mosquitoes to be infertile, using a gene drive technology that can spread the trait rapidly. The goal is to reduce the spread of malaria parasites, which infect over 200 million people annually and cause 430,000 deaths.
Researchers at the John Innes Centre successfully edited genes in two UK crops using CRISPR technology. The edits were preserved in subsequent generations, allowing for the development of disease-resistant crops. Additionally, the study found that off-target edits occurred occasionally but could be minimized by using specific guide RNAs.
Researchers have identified a novel virulence factor that could be ripe for drug development, targeting the electron transport process in TB. The discovery of MenJ, a gene essential for the organism's proliferation, offers hope for rapid treatment of the disease.
Scientists have developed a new technology that significantly improves the ability to target specific faulty genes and edit them with healthy DNA. The method reduces off-target DNA binding, allowing researchers to produce gene editing tools that are 100 times more specific for their target sequence.
A new technique has been developed to create tens of thousands of precisely guided probes covering an organism's entire genome for less than $100 in supplies. This enables genetic screening potentially accessible to organisms less well studied, facilitating targeted gene therapy and disease discovery.
Researchers at University of Birmingham discovered how mutated FLT3 genes reprogram blood stem cells, leading to abnormal cell production. The findings provide hope for developing new treatments for acute myeloid leukaemia.
Researchers at Harvard University and UC San Diego have developed a new software that predicts the most active guide RNAs for specific gene targets, facilitating faster and more efficient genome engineering experiments. This breakthrough has the potential to accelerate discoveries in gene therapies and basic genetics research.
Researchers have developed a predictive software that can identify the most effective ways to target genes with CRISPR-Cas9. The software hierarchically ranks guide RNA effectiveness based on experimental data from human genomes, speeding up the gene-editing process and improving accuracy.
Researchers at NHGRI create high-throughput gene editing system in zebrafish, allowing for simultaneous targeting and mutation of multiple genes. This method accelerates discovery of gene function and identification of disease genes in humans.
A highly efficient CRISPR/Cas system has been developed for targeted long cassette insertion into the mouse genome, achieving efficiency of up to 50%. This breakthrough technology enables the creation of humanized mice for modeling genetic diseases and improving gene therapy safety.
Scientists at Rockefeller University successfully adapted CRISPR-Cas9 gene editing to the Aedes aegypti mosquito, which transmits hundreds of millions of people annually with deadly diseases. The researchers aim to understand how different genes help the species operate as a disease vector and create new ways to control its spread.
A new study by KU Leuven researchers found that spousal loss triggers loneliness and subsequent depressive symptoms. The study suggests targeting key symptoms like loneliness can prevent further symptom activation and full-blown depression.
Duke researchers demonstrate a genetic therapeutic technique targeting a large region of the dystrophin gene to treat up to 60 percent of DMD patients. The CRISPR system is used to cut specific exons, altering the gene and producing a shortened dystrophin protein.
Researchers at Duke University have devised a method to activate genes in specific locations using light, allowing for precise control over genetic expression. This technology has the potential to revolutionize genetic engineering and may lead to breakthroughs in tissue engineering and regenerative medicine.
Leading scientists have developed a research strategy to expand Geroscience research, focusing on integrating 'pillars of aging' into chronic disease research. The goal is to extend lifespan and healthspan, while offsetting the economic burdens of an aging population with multiple chronic diseases.
Researchers developed a system to identify high-affinity nanobodies, which can precisely target specific molecules. This allows scientists to select the best nanobodies, eliminate cross-reactive ones, and generate super-high-affinity dimers for therapeutic or diagnostic applications.
Researchers identify two microRNAs that suppress NB domain genes in resistant tomato cultivar Motelle, allowing it to fight off the wilting fungus. The findings shed light on the molecular basis of plant immunity and suggest a new approach for breeding disease-resistant crops.
Researchers have found that non-coding RNA studies can improve crop resistance to pathogens and pests, reducing the need for chemical pesticides. This new approach uses small RNAs to repress the expression of target genes, promoting healthier plant development and improved nutritional value.
Facelock, a new password alternative, utilizes human face recognition to provide secure authentication. By leveraging the strengths of human memory, it eliminates the need for memorization and makes the system naturally robust.
A team of genome scientists has identified a 'hit-and-run' mechanism that allows regulatory proteins to initiate gene activation. This discovery has potential practical applications to improve nitrogen responses in crop plants, reducing fertilizer runoff and environmental health risks.
Recent advances in genetic research offer new hope for treating coronary artery disease by identifying specific genetic variants and pathways associated with cardiovascular risk. Human genetic data suggest that targeting LDL-C and triglycerides may be effective in reducing major cardiovascular events.
Researchers have sequenced the termite genome, revealing unique genetic targets for better control. The study aims to develop more specific and environmentally friendly solutions to combat termite infestations.
A recent study revisits the phenomenon of 'gaydar' in women, finding that lesbians are better at detecting sexual orientation, while straight women excel at identifying emotions and thoughts. The research also highlights differences in judgments between lesbian and straight judges.
Researchers achieved precise gene modification in monkeys using CRISPR/Cas9 system, enabling simultaneous disruption of two target genes without off-target mutations. This breakthrough generates invaluable human disease models, advancing therapeutic strategies in biomedical research.
Researchers have developed a comprehensive library of guide RNAs that can be used to identify the role of every gene in different cell types. This library was created using CRISPR technology and found that 50 out of 52 guide RNAs successfully cut both copies of specific genes, leading to a thorough understanding of how resistance occurs.
A new study using phylogenetic analysis explores the relationships among different folktales, including 'Little Red Riding Hood', to identify distinct groups spread across regions and cultures. The research suggests that folktales evolve gradually over time, with elements blending together as they spread.
Researchers developed a new method to control genes by targeting transcription, allowing for positive and negative regulation with the same protein. The technique has the potential to enable complex synthetic biology circuits and applications such as disease detection and drug production.
Scientists sequenced entire genomes of high-altitude individuals and found 11 regions with significant differences between those with chronic mountain sickness and healthy controls. The study identified two genes, SENP1 and ANP32D, which were expressed more in individuals with the condition in response to low oxygen levels.
Researchers have developed a new technology that can quickly turn genes on and off by shining light, enabling precise control over gene expression. This innovation has potential applications in understanding learning and memory, as well as studying epigenetic modifications.
A study by UC Riverside researchers identifies the endoplasmic reticulum (ER) as the site of action for miRNA-mediated gene silencing. The ER, a cellular organelle, plays a crucial role in regulating gene expression through translation inhibition.