Articles tagged with Dna Strands
Researchers have developed a new method using terminal deoxynucleotidyl transferase (TdT) enzyme to produce precise, high molecular weight synthetic biomolecular structures. These structures can be tailored to create single-stranded DNA for self-assembling into ball-like containers for drug delivery or incorporating unnatural nucleotides.
Engineers at FAU have successfully produced complex crystal lattices, so-called clathrates, using DNA strands and nanoparticles. The team achieved this by reordering pyramid-shaped gold crystals to form clathrate compounds through a self-assembling process.
The study reveals a left-handed coil structure of the Mcm2-7 hexamer and Cdt1-MCM heptamer, shedding light on DNA unwinding mechanisms. The open-coil structure has profound implications for understanding DNA replication initiation and elongation.
Researchers created self-assembled 3D DNA crystals with moveable components to change their contents after formation. The innovation enables the manipulation of internal crystal contents in 3D systems, promising advancements in nanomechanical devices.
A team of researchers at Caltech has developed a software tool called Seesaw Compiler that allows users to design and build DNA circuits with ease. The tool uses a systematic wet-lab procedure to guide researchers through the process, making it accessible to novices like undergraduate students.
Adaptive PCR, a new method developed by Vanderbilt University biomedical engineers, uses left-handed DNA to monitor and control PCR reactions. This approach promises to simplify PCR operation, improve reliability, reduce sensitivity to environmental conditions, and enable handheld size.
Researchers have solved the mystery of DNA replication by identifying a ring of proteins that binds to origin DNA, causing it to melt and initiate replication. This discovery could lead to understanding genetic duplication and potentially blocking viral pathogens and cancer cells.
Scientists have created clusters of spherical compartments by mimicking natural organelle structures. The synthetic compartments were connected using DNA bridges, enabling controlled properties and architecture.
A new study by Duke University researchers creates an analog DNA circuit that can add, subtract and multiply in a test tube, using concentrations of specific DNA strands as signals. The technology has the potential to be used in diagnosing and treating diseases, with applications including sensing vital signs and detecting molecular si...
Researchers at UC Berkeley discovered a way to boost CRISPR-Cas9 cutting efficiency up to fivefold by disrupting DNA repair mechanisms with short oligonucleotide pieces. This technique increases the success rate of creating knockouts, essential for studying gene function and correcting hereditary mutations.
Researchers are using DNA origami to create large, two-dimensional honeycombs and tubes with precise structures. They aim to develop new medicines by exposing the immune system to DNA origami scaffolds holding virus pieces, and explore protein arrangements for sophisticated medicines and electronic devices.
A team of researchers has engineered a DNA nanowire with alternating guanine bases to facilitate long-range wave-like electronic motions. This breakthrough may lead to the development of stable, efficient, and tunable DNA nanoscale devices.
Researchers have developed a framework to manipulate DNA's conductivity by varying its sequence, length, and stacking configuration. This enables the creation of stable and efficient DNA nanowires with potential applications in gene damage identification and novel electronics.
Researchers developed a new enhanced DNA imaging technique that can probe individual DNA strands at the nanoscale, providing orientation information and rotational dynamics. The technique offers more detailed information than current methods, enabling monitoring of DNA conformation changes and interactions with proteins.
Researchers developed an electrical graphene chip capable of detecting DNA mutations at high resolution. The technology could be used in various medical applications such as blood-based tests for early cancer screening and real-time detection of viral and microbial sequences.
A new method for designing geometric forms built from DNA has been developed, allowing for the creation of tiny structures in 2 and 3 dimensions. The technique, known as DNA origami, relies on a top-down strategy and can produce virtually any polyhedral shape.
Scientists create synthetic polymers that decompose without harsh elements, opening doors for biomedical applications such as drug delivery and bioimaging. Preliminary testing shows growth and depolymerization of straight and branched polymers are possible in water and extracellular matrix.
Researchers used DREEM to visualize how DNA wraps around TRF2, forming T-loops that protect chromosome ends. The new technique provides insights into telomere maintenance and structure-function relationships.
Researchers found that APOBEC3 enzymes target the lagging-strand template during DNA copying, causing C-to-T mutations. This discovery sheds light on a major source of mutations driving tumor growth and explains microbial evolution.
Researchers at McGill University have developed a method to assemble gold nanoparticles using DNA structures, allowing for the creation of novel materials with unique properties. This 'printing press' for nanoparticles has the potential to facilitate use in electronic and medical applications.
At different hydration levels, researchers found that water contributes to subpicosecond structure fluctuations and broadens vibrational transitions in DNA. The study also reveals a pronounced coupling of backbone modes and an energy transfer between them.
Researchers have identified a previously unknown protein that plays a vital role in DNA repair, potentially leading to new biomarkers and treatments for cancer. The discovery could help prevent errors during the repair process, reducing the risk of mutations and cancer.
Researchers have developed a new way to detect chemical damage to DNA that can lead to genetic mutations and diseases. The method combines existing techniques to mark and copy DNA damage sites, preserving information on the location and type of damage.
A team of researchers has revealed the molecular architecture of the replisome, a complex responsible for unwinding and replicating DNA in eukaryotic organisms. The findings show that the replisome has a unique structure, with one polymerase sitting above the helicase, challenging decades-old textbook drawings.
Researchers at Imperial College London have created a fluorescent molecule that can reveal the presence of quadruplexes in living cells. This breakthrough could be a game changer to accelerate research into these DNA structures and identify new compounds that can bind to them, potentially leading to new cancer treatments.
Scientists at Kyoto University developed an approach to assemble DNA origami units into larger structures by using a double layer of lipids. This method allows for more freedom of movement and interaction between origami structures, enabling them to form nanomachines such as nanomotors for targeted drug delivery
Researchers from North Carolina State University have discovered how two important proofreader proteins, MutS and MutL, work together to signal the body's repair mechanism. The proteins use a unique communication system involving PCNA, which helps them identify and correct errors during DNA replication.
Researchers created bundles of double-helix molecules and used them to form a rigid framework, then added complementary strands to glue nanoparticles in place. This method produced predictable clusters and arrays with tailored structures and functions.
Researchers at Brookhaven National Laboratory have developed a method to selectively rearrange nanoparticles in three-dimensional arrays, producing different configurations or phases from the same nano-components. This allows for dynamic control over material properties, such as response to light or magnetic fields.
The McGill team has devised a method to create longer DNA strands, including custom-designed sequence patterns, using an enzyme called ligase and polymerase. This approach produces large amounts of these longer strands in just a few hours, making the process potentially more economical and commercially viable than existing techniques.
Researchers developed a method to fabricate structured composite materials using directional bindings of shaped particles for predictable assembly. The approach uses linker molecules made of complementary strands of DNA to control the arrangement of particles, achieving long-range order in large-scale assemblies and clusters.
Researchers at Johns Hopkins Medicine have created a 3D model of the ORC protein machine, which helps prepare DNA for duplication. The model reveals that ORC is not always 'on' as previously thought, and no one knows how it turns on and off.
Researchers at McGill University have developed a new DNA nanotube assembly method that allows for better control over size and structure. This breakthrough could lead to applications in opto-electronics and smart drug-delivery systems.
Researchers at Brown University have developed a tiny DNA 'cage' that can trap and hold a single DNA strand after it's been pulled through a nanopore. This allows for the first-time detection of chemical reactions on a single molecule, enabling new biochemistry experiments.
Researchers developed a new self-assembled material that can amplify small variations in temperature and concentration of biomolecules, making it suitable for biosensors and drug delivery systems. The material's unique response to changes in temperature and concentration could lead to significant advancements in sensing technology.
Researchers at Ohio State University have designed DNA origami machines that can perform tasks repeatedly, using natural and synthetic DNA to mimic macroscopic machine design principles. The machines can detect signals, process information, and respond accordingly, opening the door for complex nano-robots in biomedical applications.
Researchers used time-lapse crystallography to show that DNA polymerase inserts damaged molecules into DNA strands, triggering cell death in response to environmental exposures. This process can lead to various human diseases, including cancer, diabetes, and cardiovascular disease.
Researchers discovered that DNA scaffolding plays a crucial role in controlling gene expression by forming topologically associated domains. These domains contain super enhancer regions that enhance or repress gene activity.
Researchers use simulation techniques to characterize the mechanisms of knot formation in DNA strands as a function of nano-channel diameter. Below 50 nanometers in diameter, knot formation decreases dramatically.
A new technique called Convex Lens-Induced Confinement (CLIC) enables researchers to rapidly map large genomes while clearly identifying specific gene sequences from single cells. This breakthrough could lead to faster diagnosis of diseases like cancer and pre-natal conditions.
Researchers simulated DNA knots and their dynamics using electric fields and optical tweezers, enabling controlled movement of the knot. This study provides useful information for setting up new experiments to control DNA knot movement.
The study reveals that two kinases, Plk1 and CDK, work together to ensure precise CENP-A replenishment at centromeres. Incorrect timing of this process can lead to chromosome segregation failure, resulting in cell death or disease.
Scientists have discovered new fragmentation pathways that occur universally when DNA strands are exposed to metal ions, leading to the creation of charged intermediates. This finding could contribute to optimizing cancerous tumour therapy by improving understanding of how radiation interacts with complex DNA structures.
Computer simulations show that two knots on a DNA strand can interchange positions through a growing and diffusing knot mechanism. The swapping of positions is relevant for future technologies like nanopore sequencing, where long DNA strands are sequenced by being pulled through pores.
Bleomycin's ability to cut through double-stranded DNA in cancerous cells leads to cell death. The new study presents alternative biochemical mechanisms for DNA cleavage by bleomycin.
Researchers at the University of Edinburgh have discovered a set of proteins that stabilise cell division, which could lead to new avenues in drug discovery for fighting cancer. The findings shed light on how cells duplicate their DNA and separate into two new cells, each identical to the original.
Researchers at Johns Hopkins have identified a pathogen sensor called IFI16 that plays a crucial role in recognizing viruses and bacteria. The study reveals that IFI16 uses the length of DNA as a molecular ruler to distinguish self from non-self, which could lead to new treatments for autoimmune disorders.
A collaborative team, led by Prof. Mark Williams, reveals how the APOBEC3G protein forms a roadblock to prevent HIV replication, offering new avenues for HIV therapy and drug development.
Researchers studied the release of genetic material from viral capsids into host cell nuclei, finding that highly ordered DNA strands exit faster than tangled ones. The study's findings have implications for designing artificial viral vectors and understanding complete DNA stalling in experiments.
Researchers at Penn University have developed a new technique for fast and sensitive DNA sequencing using graphene nanoribbons with nanopores. The team's innovative method allows for faster measurement of DNA sequences, as the electrical current flowing through the ribbon is modulated by each base.
Researchers at University of Michigan and Jiangnan University have developed a new method for detecting DNA using twisted light, achieving 50 times better sensitivity than current methods. This technology has the potential to aid in diagnosing patients, solving crimes, and identifying biological contaminants.
Researchers at Brookhaven National Laboratory have created a method for combining different types of nanoparticles to produce large-scale composite materials. By using DNA-based assembly methods, they can control and optimize the properties of newly formed materials.
Researchers at McGill University have created DNA 'cages' that can encapsulate small-molecule drugs and release them in response to a specific stimulus. The discovery has the potential to revolutionize drug delivery methods, offering precise control over drug release and reducing toxicity.
A team of scientists has identified a potential mechanism for cellular memory, which allows cells to recall the order of transcription factor binding. This discovery sheds light on how cells maintain gene regulation and may have implications for understanding diseases such as cancer.
University of Texas Medical Branch researchers have figured out how mammalian cells repair damaged bases in the single-stranded genome. The 'cowcatcher' enzyme, NEIL1, rides in front of the replication complex to scout for damage and stalls machinery until it's repaired.
A team of University of Pennsylvania physicists has made progress in the development of a new gene sequencing technique using solid-state nanopores. The researchers successfully differentiated single-stranded DNA molecules containing sequences of a single repeating base, achieving a promising breakthrough in this area.
Researchers have invented a new way to zip and unzip DNA strands using electrochemistry, enabling fast control at constant temperatures without dramatic changes in solution conditions. This method uses DNA intercalators that bind differently to DNA depending on their electrical state, allowing for rapid and precise control.
Researchers at ASU's Biodesign Institute have developed novel 2-D and 3-D DNA nanotechnology structures that push the boundaries of complex molecular designs. By re-engineering the Holliday junction, they created flexible geometries for building in three dimensions, enabling multilayered and curved objects.
Researchers expose laboratory-grown human skin to intense THz radiation and detect DNA damage markers. They also observe increases in tumor-suppressing proteins facilitating DNA repair.
Researchers at Brown University found that DNA molecules are more likely to be captured at or near an end than in the middle when pulled through a solid-state nanopore. The discovery is attributed to the application of polymer network theories, including Jell-O theory, which predicts more configurations with ends facing the pore.