Articles tagged with Cell Cultures
A novel gene knock-in technique using PITCh enables accurate and efficient insertion of exogenous genes into human cells, silkworms, frogs, and other organisms. This method overcomes technical hurdles associated with homologous recombination in cultured cells and organisms.
A study published in Scientific Reports reveals that CCL2 can activate the JAK/STAT pathway and increase stem cell colony attachment, differentiation efficiency, and X chromosome reactivation. The researchers also found higher expression of genes related to hypoxic response, suggesting a potential link between cellular stress and pluri...
Researchers at the University of Toronto have developed a tool that allows for cost-efficient, three-dimensional microgels to study cells in a naturalistic manner. The digital microfluidics platform enables flexible incorporation of different cell types and shapes, with potential applications in personalized medicine.
Human mesenchymal stem cells can undergo spontaneous tumorigenic transformation during cell culture expansion, a finding with significant implications for their clinical application. Researchers identified RNA molecule signatures that may serve as biomarkers to detect these aberrant cells.
Many clinical studies on vitamins have poor methodology, leading to inaccurate conclusions and defying existing evidence. New methodologies are needed to provide scientifically valid information to consumers with inadequate diets or deficiencies.
Researchers found that parasitic DNA elements proliferate in mouse tissues as they age, particularly in cancerous tumors. Calorie restriction can reduce this activity, suggesting a potential target for preventing chronic diseases associated with aging.
Researchers at Brown University have identified a small molecule compound called Retro-2 that successfully controls the spread of three polyomaviruses in human cell cultures. The compound protects up to 90.5% of cells from infection, offering new hope for treatment options.
Researchers have developed a method to isolate and grow pancreatic stem cells from mice, which can differentiate into hormone-producing beta cells or pancreatic duct cells. This breakthrough could lead to the development of new therapeutic interventions for pancreatic diseases like diabetes.
A new hypothesis suggests that spherical structures in the nucleus, containing FE65 and BLM proteins, can give a wrong signal for cell division. This degeneration and death of nerve cells is linked to Alzheimer's patients.
A groundbreaking study by Mügen Terzioglu and colleagues challenges long-held ideas about the protein MTERF1's importance in mitochondrial transcription and translation. The findings, published in Cell Metabolism, demonstrate that MTERF1 is not as crucial to mitochondrial function as previously thought.
A new model of cell fusion was created by researchers at Johns Hopkins, revealing two critical components necessary for the process. The discovery may lead to improved treatments for muscular dystrophy, as cell fusion plays a crucial role in muscle regeneration.
Researchers from Polytechnic University of Catalonia develop a low-cost electroporation system for cell cultures, reducing stress on cells and costs by up to 90%. The new system enables safer and more efficient gene therapies and molecular biology experiments.
Scripps Research scientists have devised a powerful new technique to discover and expand the power of large numbers of antibodies, immune system proteins that detect and destroy invaders. The method rapidly identifies antibodies with useful biological activity, enabling the creation of unusual antibodies with desired effects.
The study found that diesel soot triggers a genetic detoxification mechanism in human lung cells but also contains toxic substances like carboxyl groups. The World Health Organization has reclassified diesel soot as a cause of lung cancer based on sufficient evidence.
Researchers develop a new method to isolate and grow cancer cells, which can aid in understanding how cancer spreads and ultimately fighting it. The soft fibrin substrate promotes the growth of tumorigenic cells, making them more efficient at causing tumors.
Researchers found that fresh islet cells are superior to cultured islet cells in terms of success rate and blood glucose levels after transplantation. However, the results of islet isolation did not correlate with clinical outcomes for kidney transplantations from non-heart-beating donors.
Engineers developed glucose-sensing microbeads using droplet microfluidics, allowing for non-invasive monitoring of cell cultures. The technique enables detection of local glucose concentrations and gradual changes due to cell metabolism.
Researchers successfully established cell cultures from frozen biopsy specimens of the critically endangered Mississippi gopher frog, marking a breakthrough in banking viable amphibian cells. The technique expands conservation efforts to combat diseases and advance reproductive studies.
The ePetri system revolutionizes cell culture experiments with real-time imaging capabilities, reducing human labor time and improving accuracy. It simplifies medical diagnostic tests, drug screening, and basic research, offering a compact, lightweight microscope alternative.
Scientists at NIST have developed a method to capture metal-based nanoparticles on a surface and release them at the desired moment, allowing for effective assessment of their toxicity in cell cultures. This approach enables precise delivery of particles to cells, mimicking real-world encounters, and reducing clumping issues.
A UBC-led team has developed an automated microfluidic cell culture platform to study hundreds of hematopoietic stem cells at the single cell level. The new tool provides insights into HSC survival and growth factor requirements, with applications in drug development, clone selection, and culture optimization.
Researchers at KIT have successfully cultivated cells on three-dimensional structures with precise control over adhesion and cell shape. The team developed a special polymer scaffold using the Direct Laser Writing Method, which allows for the growth of individual cells in specific locations.
Researchers at the Monell Chemical Senses Center have successfully maintained human taste cells in culture for seven months, providing a valuable tool for understanding the sense of taste. The breakthrough enables scientists to test drugs to promote recovery from taste loss due to infection, radiation, or chemotherapy.
A new cell-culture-derived flu vaccine has shown to be at least as immunogenic and efficacious as traditional egg-derived vaccines. The vaccine was well-tolerated with no treatment-related serious adverse events, and its production allows for more reliable and flexible supply.
A fully defined culture system has been developed to grow human embryonic stem cells in the lab, reducing guesswork and increasing safety. The system uses a synthetic substrate and defined growth medium, allowing for up to three months of cell culture with minimal batch-to-batch variability.
A protocol for detecting targeted chromosomal deletions induced by zinc finger nucleases allows researchers to estimate the frequency of ZFN-induced genomic deletions using PCR-based methods. Immunoprecipitation is also optimized with detailed instructions for lysis conditions, protease inhibitor cocktails, and general preparation.
Researchers have developed a new method for growing 3-D cell cultures using magnetic levitation, which more closely resembles the body's natural tissue structure. The technique has shown promising results in preclinical drug tests and may revolutionize cancer research.
A new 3-D cell culture technique using magnetic forces has been developed, allowing for the growth of cells in 3-D. This method promises to provide more accurate preclinical drug tests and better cancer research outcomes by mimicking the body's natural tissue structures.
A two-part special issue published in the journal Homeopathy explores experiments on homeopathic treatments using biological models, including whole animals and plants, cell cultures, and enzymes. The study found that seven different biological models of high dilution response yielded consistently positive results.
Researchers at UCF have developed a method to optically steer cells using low-intensity polarized light, guiding them towards desired locations. This technology has the potential to improve stem cell therapies for wound healing and cancer treatment.
A specific DNA change has been identified as a potential mechanism for developing schizophrenia. The research found that the altered gene increases expression in cells grown in culture, echoing findings in postmortem brain samples from individuals with schizophrenia.
A new synthetic gene circuit allows for precise dosing of gene expression in yeast cells, enabling accurate analysis of a gene's role in normal and abnormal cellular function. The circuit utilizes negative feedback loops to achieve a linear dose-response relationship.
Biomanufacturers are developing fully disposable bioprocess streams to minimize validation studies, sterilization, and cleaning procedures. Companies like GE Healthcare and Pall offer disposable products that reduce inventory costs and improve flexibility in bioprocessing.
The journal Cold Spring Harbor Protocols presents two articles detailing experimental culture methods for cells from the immune system and the nervous system. These protocols enable researchers to isolate and grow specialized cells like macrophages and cerebellar granule neurons, facilitating studies on their functions and interactions.
Researchers at Washington University School of Medicine have identified a blood-borne immune system compound that suppresses antibody-dependent enhancement (ADE) of infection, a phenomenon linked to some viral disease outbreaks. C1q, a key immune compound, blocks ADE by blocking the virus' ability to hijack antibodies.
Researchers at Johns Hopkins University developed a thumb-size micro-incubator that cultures living cells autonomously for extended periods. The device features computer-controlled electronics, precise temperature control, and an eco-friendly design that minimizes environmental impact.
A team of Brown University biomedical engineers has invented a 3-D Petri dish that can grow cells in three dimensions, enabling the quick and cheap production of realistic cells for drug development and tissue transplantation. The technique employs a new dish made from a sugary substance that allows cells to self-assemble naturally and...
Researchers found that cells cultured in three dimensions exhibit distinct gene expression patterns, growing faster and exhibiting more realistic shapes. The findings suggest that 3-D cell culture methods may be a better representation of the human body's complex cellular environments.
A recent study found that stem cell identity in culture is highly dependent on the surrounding environment, challenging traditional views of differentiation. Researchers suggest that this context-dependent approach could lead to more efficient stem cell culture methods and potentially even develop stem cells from differentiated cells.
Researchers at the University of Pittsburgh School of Medicine have discovered a potent and selective killer of leukemia cells, specifically cyanidin-3-rutinoside, a naturally occurring compound found in many fruits and vegetables as well as red wine. This finding offers hope for a more targeted and less toxic therapy for leukemia.
Researchers from Massachusetts General Hospital discover that isoflurane, a commonly used anesthetic, can lead to generation of amyloid-beta protein and apoptosis in brain cells. The study suggests caution when using the anesthetic for elderly patients at risk of Alzheimer's disease.
Researchers have developed a new 3D scaffold made of protein nanofibers that can support the growth and differentiation of stem cells. The scaffold provides a more accurate representation of the natural context of cells in tissues and organs, and has the potential to replace traditional Petri dishes for growing cells.
Researchers propose using existing facilities to produce vaccines from cell cultures, which could make enough doses to cover the US in just 3-4 months. This approach would reduce downtime and capital investments required for new dedicated facilities.
Embryonic stem cells diversify to form various neural structures when cultured on different surfaces, with laminin influencing specific pathways crucial for brain cell generation and survival. Laminin's role in directing stem cells to become specialized neurons sheds light on fundamental mysteries of brain development.
NIST researchers create a microfluidics technique to isolate and pattern neuronal cells on surfaces, allowing for the study of cell development and behavior. This breakthrough enables a variety of cell-geometry experiments, such as measuring the maximum gap between lines that can be bridged by neural axons and dendrites.
Researchers at the University of Manchester have created a new bio-gel for 3D cell culture that mimics natural cell scaffolds. The gel, made from dipeptides, has potential applications in wound healing and regenerative medicine.
Researchers have successfully created living taste cells in a lab culture, opening new avenues for understanding the sense of taste and potentially treating disorders. The breakthrough could lead to new insights into how basal cells turn into functional taste cells.
Researchers created a simplified version of cardiac tissue using cells from neonatal rats, mimicking normal heart tissue. The study found that pace-setting pulses successfully halted the abnormal rhythm 80% of the time, but in 20% of cases, it worsened the condition.
Researchers at Rice University have developed a method to reduce the toxicity of water-soluble carbon nanotubes through surface modifications. The study found that even minor changes can dramatically decrease cytotoxicity, making these nanoparticles more suitable for medical diagnostics and imaging.
Researchers at the University of Florida have successfully generated brain cells in a dish, a breakthrough that could lead to new treatments for neurological disorders. The discovery identifies the true stem cell, which can be used to produce a limitless supply of brain cells to potentially heal damaged brain function.
Scientists have developed a method to create an infectious form of the hepatitis C virus in a test tube, allowing researchers to study its life cycle and develop new drugs. This breakthrough could lead to better understanding and treatment of liver diseases associated with HCV.
Researchers identified a dozen potential facilities worldwide that could manufacture flu vaccines in emergency situations. Cell-based vaccines have the potential to reduce production time and improve flexibility compared to traditional egg culture methods.
Research found soy infant formula's high concentration of genistein may hinder intestinal cell proliferation, a critical organ system development phase. However, clinical data suggests soy formulas are safe but may have unseen effects.
Researchers at Johns Hopkins University have found a possible link between the strange electrical waves in their hearts and the cause of accelerated heartbeats. They discovered that when implanted cardioverter defibrillators triggered a series of unusual electrical pulses, they sometimes triggered the formation of these same spiral waves.
Researchers at UNC have developed a high-throughput technique to rapidly analyze gene functions, resolving two bottlenecks in the process. This method has the potential to accelerate the development of new drugs by quickly identifying and testing candidate genes.
Researchers at WashU Medicine have successfully grown cells known as Tr1 cells in the laboratory, which can suppress immune responses. This breakthrough could lead to new treatments for autoimmune diseases such as lupus and rheumatoid arthritis, as well as provide insights into infectious diseases like measles and meningitis.
Researchers found a way to grow hepatitis C virus in labs, enabling genetic studies and potential vaccine development. The breakthrough could speed up progress against the disease, which affects 170 million worldwide.