Articles tagged with Cell Cultures
This study investigated the effect of culturing hepatocellular cancer cells in more native conditions, revealing slower doubling times, increased glycolytic enzymes, and mutations in mitochondrial DNA. The results showed increased resistance and sensitivity for cells cultured in native conditions, highlighting new insights into tumor b...
Researchers at NIH have devised a four-part small-molecule cocktail, CEPT, that protects induced pluripotent stem cells from stress and maintains normal stem cell structure and function. The cocktail enhances the potential therapeutic uses of stem cells in treating diseases like diabetes, Parkinson's disease, and spinal cord injury.
The Cell Culture Engineering XVII Conference has been postponed due to COVID-19, prompting two webinars to be held instead. The first webinar focuses on vaccines, highlighting their safety, efficacy, and global distribution. The second webinar explores therapeutic approaches using antibodies and biologics to neutralize the virus.
Researchers at the University of Miami have successfully cultured cells from coral and sea anemones for up to 12 days, opening new avenues for studying evolutionary biology and human health. The breakthrough uses antibiotic treatment to prime tissues before culture, allowing for longer and more robust cell cultures.
A new technique developed by Brown University researchers reveals the forces involved at the cellular level during biological tissue formation and growth processes. The technique makes use of cell-sized spheres made from a highly compliant polymer material, which can be placed in laboratory cultures of tissue-forming cells.
Corning Life Sciences is sharing its latest 3D cell culture technologies at SLAS2021 to support critical research in the industry. The company's new products, including Matrigel matrix-3D plates and Corning Elplasia plates, feature microcavity technology for high-volume spheroid production and enable rapid imaging of 3D cultures.
Researchers at the University of Maryland are developing an ingestible capsule that can monitor and model gut microbiome serotonin activity. The platform provides access to the specific site of serotonin production, allowing for real-time processing and neurological effects assessment.
Scientists from Tokyo University of Science have discovered a natural food pigment Monascus purpureus that can distinguish between living and dead cells. The pigment is robust against chemotherapy drugs and does not damage living cells, making it a promising tool for screening cell viability in various types of cancer research.
Scientists have created a model that replicates the progression of age-related macular degeneration (AMD), a disease with no current treatment. By understanding how RPE cells respond to stress and changes in gene expression, researchers can now test potential therapeutics for dry AMD.
Researchers found that eight commercially available mouthwashes can inactivate Sars-Cov-2 viruses in cell culture experiments. The use of these mouthwashes could potentially reduce the viral load and risk of transmission, particularly in high-risk areas like the oral cavity and throat.
Researchers at UC San Diego have developed a reliable method to culture human sperm stem cells, overcoming a key bottleneck in SSC therapy. The approach uses AKT inhibitors to maintain the growth of immature germ cells with SSC characteristics, paving the way for potential clinical applications.
A recent study published in Scientific Reports found that silicone molecules from breast implants can initiate processes leading to cell death in human cells. The researchers used cultured cells and observed similarities with programmed cell death, which may have implications for the health effects of silicone breast implants.
Researchers at the National Eye Institute have discovered a tooth-enamel protein, amelotin, that is also present in eyes with dry age-related macular degeneration. The protein may play a role in the formation of calcium deposits in the eye and could be a therapeutic target for treating the blinding disease.
New study finds low doses of ionizing radiation used in medical imaging create breaks that allow extra bits of DNA to integrate into the chromosome, potentially leading to mutations. The researchers stress that translating these findings to animal models is necessary to determine full effects and impact on patient health.
Corning is showcasing its advanced 3D cell culture portfolio, including Corning Elplasia plates and Matrigel matrix. The company will demonstrate how these tools address advancements in 3D cell culture and cell therapy research for drug screening, cancer biology, stem cell biology, and immunology applications.
Scientists identified 3 metabolites that can re-program pluripotent cells into totipotent-like cells, opening up possibilities for studying early developmental events and cell replacement therapies.
The study found that temporarily disabling SETD3, a crucial protein needed for enteroviral replication, can protect against viral diseases. In mice bioengineered to lack SETD3, the viruses were unable to infect and replicate.
Researchers have developed a novel culture method for 3D multicellular spheroids, utilizing a durable superamphiphobic surface. This method enables efficient, safe, and in-situ observation of cell growth, with potential applications in drug screening, stem cell differentiation, and regenerative medicine.
Researchers in Kyoto have developed a 'quantitative biomarker' to assess the quality of corneal cells, allowing for prediction of their long-term efficacy through simple observation. This breakthrough has potential applications in preemptive medicine, enabling clinicians to intervene before severe symptoms appear.
The switch from 2D to 3D cell cultures in drug discovery improves the accuracy of human physiological conditions and increases the success rates of drugs. High-content analysis with 3D cell cultures is expected to increase the quality of compounds progressing to preclinical stages.
Researchers at Linköping University created a hydrogel that mimics the natural environment of cells, allowing for the growth of human liver cells on microchips. This innovation has the potential to simplify early stages of drug development and replace animal experiments.
Researchers at Lobachevsky University have discovered the TrkB receptor system's role in forming neural networks. Activating this system increases complex functionally active neural networks with high transmission efficiency of nerve impulses, essential for learning and memory.
Researchers have discovered significant abnormalities in nerve connections and myelin production in cells from patients with schizophrenia. These findings suggest a potential new model for studying psychotic disorders, which could lead to better treatment approaches.
Researchers successfully established an immortalized cell line derived from the critically endangered Okinawa rail. The use of the K4DT method, which involves co-expressing mutant CDK4, Cyclin D, and TERT genes, significantly extended cellular division and immortalization in avian cells. This breakthrough paves the way for further func...
Researchers at Duke-NUS Medical School and Singapore General Hospital develop a fully human system to cultivate skin cells for grafting, eliminating the need for animal-derived products. This breakthrough method uses specific laminin proteins to support the growth of human keratinocytes in the laboratory.
Remnants of the extracellular matrix promote inflammation and airway remodeling in asthma. Accumulation of PGP may explain why LTA4H inhibitors have failed in clinical trials.
Researchers at Tohoku University have identified a new mutant coral symbiont alga that enables the control of symbiotic relationships with its host. The discovery provides a powerful tool for studying coral-algal endosymbiosis and sheds light on mechanisms for maintaining stable symbiosis.
Researchers at Helmholtz Munich have discovered a specific subset of pluripotent embryonic stem cells that can transform into totipotent-like cells in culture. This breakthrough could pave the way for new treatments and therapies for various diseases, including diabetes and lung conditions.
Researchers have introduced polyproline as an effective cryoprotectant for monolayers of cells, doubling cell recovery after freezing compared to dimethyl sulfoxide. The proline/polyproline combination minimizes solvent exposure and retains cell functions.
Researchers have discovered a way to rejuvenate inactive senescent cells, reversing telomere shortening and restoring cell division. This breakthrough could lead to therapies that promote healthy aging without degenerative effects.
A new technology bridges clinical pathology assessment, high-content analysis, and 3D cell cultures, allowing rapid, automatable, and multiplex readouts. This technique is applied to mixed arrays made up of spheroid models from various cell lines, demonstrating its potential for safety and efficacy testing and model optimization.
Researchers have created a novel cell scale that enables measuring the mass of living cells with high resolution and monitoring their weight changes over time. This allows tracking of fluctuations during the cell cycle, substance influence on cell mass, and viral infection effects.
Researchers found that exposure to forest fire pollutants caused severe DNA damage and stopped lung cells from dividing. Retene, a chemical compound in polycyclic aromatic hydrocarbons (PAHs), was the main culprit, leading to inflammation, oxidative stress, and genetic damage.
Researchers found over 30,000 publications reporting on misidentified HeLa cells, leading to potentially invalid research data and reproduced results. The study aims to raise awareness about the problem and promote caution when interpreting results.
Microglia, the brain's front line of immune defense, have been characterized for the first time. Genes linked to neurological diseases are highly expressed in microglia, suggesting a link between the cells and neurodegenerative and psychiatric illnesses.
The Moscow State University has opened a new cryobank to store biotechnological plant material, including rare species cell cultures. The facility aims to prevent cross-contamination by storing biological samples in nitrogen vapor, not liquid nitrogen.
Researchers found that soy-protein concentrate can exert antioxidant and cytoprotective effects in cultured human bowel cells and moderate inflammation in mice with induced IBD. The study suggests a potential dietary strategy for mitigating the severity of inflammatory bowel diseases.
Researchers at the University of Luxembourg have successfully grown brain-like cultures from human stem cells, mirroring the structure and function of the midbrain. This breakthrough allows for the study of Parkinson's disease mechanisms and potential treatments, as well as reducing animal testing in brain research.
Researchers found that tumor cells entering a reversible state of proliferation arrest when glutamine is depleted, rather than dying. c-MYC levels are down-regulated upon glutamine deprivation, suggesting it couples transcription to nucleotide availability.
A new survey investigates ideal qualities of microfluidics-based 3D cell culture systems, finding a positive attitude among the biomedical community. However, a gap remains between desired and available features, including complexity, user-friendliness, physiological relevance, and controllability.
A partnership between UMass Amherst and PCL Inc. has enabled researchers to screen thousands of promising molecules for potential as new drug therapies. The sol-gel capture technology allows for highly sensitive target-molecule detection in chemically complex plant samples.
Researchers developed a technology that could produce influenza B vaccines with higher efficacy, using mammalian cell culture instead of eggs. This could lead to better protection against both lineages of influenza B and improved ability to respond during an influenza pandemic.
Researchers have discovered a breakthrough in scaling up life-changing stem cell production by using a protein derived from human blood, Inter-alpha inhibitor. This method enables faster and cheaper large-scale industrial production of human pluripotent stem cells without the need for costly biological substrates.
Researchers at the University of Nottingham have developed a novel method for culturing human stem cells using a protein derived from human blood, which could lead to faster and more cost-effective large-scale production. This breakthrough has the potential to revolutionize the field of regenerative medicine and disease research.
A Buck Institute study found that boosting a broccoli-related compound, palmitoleic acid, may be a possible treatment for age-related macular degeneration. The research identified indole-3-carbinol as a starting point and used a virtual screen to find a more potent activator of the aryl hydrocarbon receptor protein.
Researchers developed a method that uses lasers to carve out paths inside biocompatible gels, locally influencing cell function and promoting tissue formation. This enables growing cells in custom-built yet biologically active 3D spaces, addressing limitations of previous approaches.
A team of researchers has developed a nanoprobe that enables the direct measurement of key proteins within living, cultured cells. The device tracked levels of Alzheimer's-disease-associated proteins amyloid-beta and tau in neurons exposed to an anesthetic, revealing that A-beta generation precedes tau phosphorylation.
Research reveals lower CD74 receptors on placental macrophages disrupt fetal nourishment, leading to abnormal placentas and preeclampsia. This discovery opens new possibilities for long-term therapy targeting root causes of the disease.
New studies show that cells in mouse mammary tissue compare notes and share chemical signals before making a move. This collective communication process increases the accuracy of cell sensing, allowing cells to detect subtle changes in their environment.
Researchers have developed fallopian tube organoids to study female genital diseases such as infertility, inflammatory disease, and ovarian cancer. These hollow spheres can culture cells from clinical fallopian tube samples, offering a new avenue for investigating disease mechanisms and developing therapies.
Scientists at Brown University have successfully cultured cells to produce extracellular matrix with strength comparable to natural tissues. By using specialized molds to guide cell growth and alignment, the researchers created ECMs with specific mechanical properties, such as collagen fibers aligned along a tendon-like structure.
Researchers found that Janus kinase inhibitors decreased factors released by human senescent cells and reduced inflammatory mediators in mice. The treatment improved physical function, including grip strength, endurance, and physical activity, in aged mice over two months.
The researchers created mini-brains using living tissue from a single rodent, which can form complex neural connections and produce electrical signals within two weeks. The approach is expected to reduce animal use in neuroscience research and make it more accessible to labs without advanced equipment.
A new approach uses cell cultures to predict chemical effects on fish growth, showing excellent agreement with in vivo experiments. The method combines cell population growth inhibition data with modeling of toxicological effects.
Researchers at LCSB have successfully grown dopaminergic neurons in a three-dimensional cell culture system. The system models the natural conditions of the brain and is significantly cheaper than existing methods, allowing for the automation of drug testing.
Brown University researchers developed new textured surfaces using graphene to better mimic the complex surroundings in which cells grow. The wrinkled surfaces influenced cell growth, with cells being elongated and aligned along the wrinkles, resembling a biologically relevant phenotype.
The Vanderbilt-Pittsburgh Resource for Predictive Toxicology aims to develop alternative approaches for toxicity testing, focusing on 3-D human cell cultures. The center will expose these cultures to a range of previously studied toxic chemicals to predict their effects on humans.
Researchers at UC San Diego School of Medicine found that culture conditions and passage methods affect genetic stability in human stem cells. The study highlights the need for quality control to ensure the safety of transplanted stem cells.
A novel gene knock-in technique using PITCh enables accurate and efficient insertion of exogenous genes into human cells, silkworms, frogs, and other organisms. This method overcomes technical hurdles associated with homologous recombination in cultured cells and organisms.
A study published in Scientific Reports reveals that CCL2 can activate the JAK/STAT pathway and increase stem cell colony attachment, differentiation efficiency, and X chromosome reactivation. The researchers also found higher expression of genes related to hypoxic response, suggesting a potential link between cellular stress and pluri...