Fluorescence Microscopy
Articles tagged with Fluorescence Microscopy
New microscopy technique reveals hidden magnetic chemistry in living systems
A University of Tokyo team developed a fluorescence imaging method to track short-lived molecular intermediates and their magnetic responses in real time. The approach isolates spin-dependent part of chemistry, revealing how magnetically sensitive intermediates appear and disappear.
A new reagent makes living brains transparent for deeper, non-invasive imaging
Researchers at Kyushu University develop a new tissue-clearing reagent, SeeDB-Live, enabling repeated, reversible, and real-time imaging of living brains at greater depth and clarity. This breakthrough allows scientists to visualize neural activity in living mice and brain slices, offering new insights into brain dynamics and function.
Functional photoacoustic microscopy reaches super-resolution by tracking red blood cells
Researchers developed a high-speed photoacoustic microscope to track red blood cells in the mouse brain, allowing for single-cell resolution imaging of microvascular structures. This enables study of cerebral small vessel disease, cognitive impairment and dementia.
Dopamine selects, astrocytes refine: a new mechanism for motor-learning circuit rewiring
A new study reveals that astrocytes actively participate in motor-learning circuit rewiring by eliminating synapses in the striatum. The research identifies MEGF10 as a key molecular mediator of this process, which is regulated by dopamine signaling and neural activity.
New fluorescent strategy could unlock the hidden life cycle of microplastics inside living organisms
A new study proposes a fluorescence-based strategy to track microplastics in real time as they move, transform, and degrade inside biological systems. This approach allows precise control over particle brightness, emission wavelength, size, and shape, enabling the tracking of microplastic life cycles from ingestion to breakdown.
Capturing the moment of organelle handoff inside living cells
For the first time, researchers have directly visualized how newly formed cellular organelles leave the endoplasmic reticulum and transition onto microtubule tracks inside living cells. The study reveals that the ER plays an active role in steering intracellular traffic.
Real-time imaging of contact between cells and between a single neuron’s extensions
Scientists from The University of Osaka have created two new fluorescent markers, Gachapin and Gachapin-C, that can visualize dynamic cell-to-cell contacts and connections within a single neuron's extensions. These indicators allow for the monitoring of complex patterns of connectivity in various cell types, including neurons.
Bis-pseudoindoxyls: a new class of single benzene-based fluorophores for bioimaging applications
Researchers have developed bis-pseudoindoxyls, compact fluorophores with red-shifted absorption and fluorescence suitable for time-resolved bioimaging. The dyes exhibit low cytotoxicity and sufficient aqueous solubility, making them ideal for live-cell imaging.
Multi-lab collaboration unites math and biology
Researchers from the University of Tennessee at Knoxville developed a new statistical method that improves analysis in single-molecule fluorescence experiments. The method combines theoretical work from mathematics with experimental work from molecular biology, allowing for more accurate and efficient data analysis.
Shedding new light on the tiny bubbles sending signals between cells
Researchers at Ohio State University developed a new approach to immobilize extracellular vesicles in a way that mimics their interactions with tissues. This allows for the study of these particles and their complex interactions with cells, enabling potential applications in disease detection, drug delivery, and biomarker discovery.
Sperm tails and male infertility: Critical protein revealed by ultrastructure microscope
Researchers have discovered a key protein structure in the germ cells of male mice that causes deformations in sperm flagellum leading to infertility. The study used ultrastructure expansion microscopy to visualize the centriole, a tiny cylindrical structure critical for sperm movement.
Stick and Glue! Researchers at IOCB Prague introduce a new biomolecule-labeling method for more precise observation of cellular processes
A team of researchers from IOCB Prague introduces a novel method for labeling molecules with fluorescent dyes, surpassing existing approaches in precision and stability. This enables scientists to track labeled molecules over long periods with high reliability, expanding possibilities for research in biology, chemistry, and medicine.
Scientists observe metabolic activity of individual lipid droplets in real time
Researchers developed a probe to visualize lipid breakdown in living cells, revealing differences in breakdown rates among individual droplets. The study found that an enzyme called ATGL drives these variations, which may contribute to abnormal lipid metabolism in liver cancer cells.
Szeged researchers accelerate personalized medicine with AI-powered 3D cell analysis
Researchers at HUN-REN Szegedi Biológiai Kutatóközpont have developed an AI-powered platform for automated 3D cell culture analysis, enabling high-precision screening of cellular models. The technology removes the limitation of throughput in personalized medicine, allowing for fast and accurate analysis of clinical samples.
High-accuracy tumor detection with label-free microscopy and neural networks
Researchers developed a new imaging method using multiphoton microscopy to rapidly identify pancreatic neuroendocrine tumors with high accuracy. Machine learning algorithms achieved 80.6% accuracy, while convolutional neural networks outperformed with accuracies ranging from 90.8% to 96.4%.
New fiber-optic method tracks Alzheimer’s plaques in active mice
Researchers developed a fiber-optic method to track Alzheimer's plaques in freely behaving mice, allowing for real-time monitoring and long-term tracking of pathological changes. The technique uses fluorescent dye to bind specifically to amyloid fibrils and provides a minimally invasive way to study disease progression.
How dense is it inside living cells?
A recent study published in Nature Communications reveals that the nucleus is less dense than the surrounding cytoplasm, despite its rich biomolecular composition. The researchers used light to probe density at microscales and found a consistent nuclear-to-cytoplasmic density ratio across eukaryotes.
Freeze-framing the cellular world to capture a fleeting moment of cellular activity
Cryo-optical microscopy captures high-resolution, quantitatively accurate snapshots of dynamic cellular processes at precisely selected timepoints. This technique enables the observation of transient biological events with unprecedented temporal accuracy.
Handheld device enables imaging and treatment of oral cancer in low-resource settings
A newly developed low-cost, handheld intraoral device combines optical diagnostics and image-guided photodynamic therapy to detect and treat early-stage oral cancer. The device shows promising accuracy and effectiveness in detecting PpIX fluorescence and monitoring treatment in real-time.
New insights into soft material deformation
A new study maps the internal behavior of soft materials when deformed, revealing localized fracture events and heterogeneous flows. The findings challenge long-standing assumptions and provide valuable insights for improving manufacturing techniques.
Diver-operated microscope brings hidden coral biology into focus
Researchers developed a cutting-edge microscope to study coral photosynthesis and health in their natural habitat. The BUMP imaging system provides unprecedented insights into coral reefs, advancing efforts to understand coral bleaching.
Viewing organs in 3D
Researchers developed a new method for imaging enzyme activity in whole organs with high-resolution 3D mapping. This allowed them to visualize differences in aminopeptidase N activity and the effects of inhibitors in mouse kidneys. The study opens up an unbiased evaluation method for drug development.
Breakthrough in rapid super-resolution imaging of multiple organelles in live cells
A team from Peking University achieved a major breakthrough in imaging 15 cellular structures simultaneously using lipid membrane probes, dual-color spinning-disk confocal microscopy, and deep learning. This method enables real-time, long-term organelle tracking with improved efficiency and reduced phototoxicity.
DNA origami guides new possibilities in the fight against pancreatic cancer
Researchers developed DNA origami structures that selectively deliver fluorescent imaging agents to pancreatic cancer cells, enabling more accurate cancer imaging and selective chemotherapy delivery. The study also explored the use of origami-folded DNA molecules loaded with chemotherapy drugs for targeted delivery to cancer cells.
Pushing the limits of brain imaging: A new tool for targeted delivery of imaging agents and drugs
A novel cannula delivery system allows repeated, nondisruptive delivery of imaging agents to the mouse brain during long-term multiphoton microscopy. This innovation enhances longitudinal studies on brain function, disease progression, and potential treatments.
Microscopy method breaks barriers in nanoscale chemical imaging
A new microscopy technique, SIMIP, combines structured illumination with mid-infrared photothermal detection to achieve high-speed chemical imaging with superior resolution. The method outperforms conventional methods in terms of spatial resolution and chemical contrast.
New imaging technology reveals inner workings of living cells
A new imaging technology has been developed that combines super-resolution imaging with artificial intelligence to reveal subcellular structures and dynamics in living cells. This breakthrough enables scientists to better understand the root causes of diseases, leading to improved treatments.
Prof. Xiaozhen Li from Northwestern Polytechnical University reviewed the rencent advancement in conjugated small molecular nanoparticles for near-infrared biomedical applications
Conjugated small molecular nanoparticles (CSMNs) have shown promise in near-infrared phototheranostics (NIR PTs) for imaging, therapy, and synergistic treatment. Strategies to improve performances and extend absorption wavelengths are crucial for their clinical translations.
Pediatric investigation study releases updated guidelines for pediatric mycoplasma pneumoniae infection
The Chinese Medical Association has released updated guidelines for pediatric mycoplasma pneumoniae infection, emphasizing a multi-faceted diagnostic approach and evidence-based treatment strategies. The guidelines highlight the importance of accurate diagnosis and responsible antibiotic use to combat rising resistance.
Color-changing fluorescent dyes unlock new frontiers in cellular thermosensing
Scientists developed a novel solvatochromic fluorescent dye that enables high-precision temperature measurements through changes in fluorescence properties. The researchers achieved exceptional sensitivity and resolution, ideal for bioimaging applications.
A closer look at biomolecular ‘silly putty’
Researchers used novel fluorogen imaging techniques to visualize biomolecular condensates, revealing distinct environmental and structural features. The study provides insights into the dynamic behavior of these condensates, which play a crucial role in various diseases.
The secret DNA circles fueling pancreatic cancer’s aggression
Researchers found that pancreatic cancer cells gain a survival edge by carrying copies of critical cancer genes on circular pieces of DNA outside chromosomes. The discovery highlights the importance of targeting extrachromosomal DNA in treating the disease.
Lighting the way: how activated gold reveals drug movement in the body
Researchers at Waseda University develop a new imaging technique that uses neutron activation to transform gold nanoparticles into radioisotopes, enabling long-term tracking of their movement in the body. This breakthrough could lead to more effective cancer treatments and precision monitoring of drug distribution.
New method uses DNA barcodes for high throughput RNA and protein detection in deep tissue
A new technique called cycleHCR uses DNA barcodes to track hundreds of RNA and protein molecules in single cells within thick biological samples. This allows researchers to decipher how genes function in different parts of an organism, how they enable development, and how they might be altered in diseases.
Fluorescence-guided surgery for hepatocellular carcinoma: From clinical practice to laboratories
Recent developments in fluorescent probes offer improved specificity and sensitivity for detecting hepatocellular carcinoma (HCC). Next-generation probes are being developed to enhance tissue penetration depth and probe specificity, potentially improving surgical outcomes.
NYU Abu Dhabi researchers develop breakthrough tool to enhance precision in cold-temperature cancer surgery
Researchers have developed a specialized nanoscale material that illuminates cancer cells under freezing conditions, improving surgical precision. This technology enhances surgeons' ability to detect and remove cancer cells during cryosurgery.
‘Fluorescent phoenix’ discovered with persistence rivaling Marie Curie’s
Researchers at POSTECH developed a super-photostable organic dye, PF555, to track proteins in cells over extended periods. This breakthrough enables observation of endocytosis and protein interactions, revealing EGFR's active navigation in its environment.
New photochemical tools based on thioketal
A new universal photocage modification strategy based on thioketal enables real-time live cell subcellular imaging. The thioketal-based probe SiR-EDT exhibits improved dark stability and can be specifically activated by UV-visible light.
New microscope can image, at once, the full 3D orientation & position of molecules in cells
A new hybrid microscope allows scientists to image the full 3D orientation and position of an ensemble of molecules, such as labeled proteins inside cells. This can reveal the real biology hidden from just a position change of a molecule alone.
Microscopy approach offers new way to study cancer therapeutics at single-cell level
Researchers developed a novel microscopy technique to study metabolic changes in individual cancer cells at the single-cell level. They found that radiation treatment caused significant metabolic shifts in head and neck squamous cell carcinoma cells, particularly through the activation of HIF-1α.
AI transforms label-free photoacoustic microscopy into confocal microscopy: A new frontiers in cell imaging technology
Researchers developed an AI-powered technology that transforms low-resolution, label-free images into high-resolution, virtually stained ones without fluorescent dyes. This innovation delivers stable and accurate cell visualization, overcoming limitations of traditional imaging methods.
New AI technique generates clear images of thick biological samples without the fancy hardware
Researchers have developed a new AI method that produces sharp microscopy images throughout a thick biological sample, count cells more accurately, and trace vessels in embryos. The technique doesn't require additional equipment beyond a standard microscope and is more accessible than traditional adaptive optics techniques.
Images of crucial cell receptors show promising new drug targets
Researchers at UChicago captured complete images of adhesion G protein-coupled receptors, revealing their complex extracellular region's interaction with the transmembrane region. The findings suggest alternative means of activating the receptor without separating the GAIN domain.
'Strong’ filters – Innovative technology for better displays and optical sensors
The study creates ultra-stable thin-film polariton filters with exceptional angular stability, transmitting up to 98% of light, even at extreme viewing angles. This technology has enormous scientific and economic potential for applications in display technology, sensor technologies, biophotonics, and more.
New imaging platform developed by Rice researchers revolutionizes 3D visualization of cellular structures
Researchers at Rice University developed soTILT3D, an innovative imaging platform that enables fast and precise 3D imaging of multiple cellular structures while controlling the extracellular environment. The platform improves upon conventional fluorescence microscopy by reducing background fluorescence and increasing imaging speed.
Rice’s Center for Nanoscale Imaging Sciences hosts inaugural workshop, uniting national experts to advance imaging across scales
The inaugural workshop at Rice University's Center for Nanoscale Imaging Sciences brought together leading experts to explore advancements in cutting-edge imaging techniques. The event integrated diverse imaging modalities to uncover new insights into biological and materials systems.
Megapixel fluorescence microscopy through scattering layers made simple
Researchers introduce a new approach for megapixel-scale fluorescence microscopy through complex scattering media, resolving high-resolution images without requiring specialized equipment. This technique efficiently corrects distortions caused by light scattering, enabling clear imaging of dense targets.
In 10 seconds, an AI model detects cancerous brain tumor often missed during surgery
Researchers developed an AI-powered model called FastGlioma that can detect residual tumor tissue with high accuracy in 10 seconds. The technology outperformed conventional methods, reducing the risk of missed tumors by nearly 75%. This innovation could change the field of neurosurgery and minimize reliance on radiographic imaging.
Bioluminescent proteins made from scratch enable non-invasive, multi-functional biological imaging
Scientists have designed bioluminescent proteins that can produce multiple colors of light for real-time imaging in cellular and animal models. These proteins are small, efficient, highly stable and can be used for non-invasive bioimaging, diagnostics, drug discovery and more.
New imaging technique to improve head and neck cancer surgery
Researchers developed a new imaging technique using fluorescence-guided surgery to enhance visibility of tumors and nerves during head and neck cancer surgery. The technique uses two near-infrared fluorophores, one for tumors and another for facial nerves, allowing for clear differentiation between cancerous tissues and nerves.
Novel artificial intelligence-based method for pathological diagnosis of hereditary kidney diseases
Researchers developed an AI-based method to analyze kidney lesions in female patients with Alport syndrome, predicting renal prognosis and guiding treatment interventions. The approach uses a modified stain and deep learning to detect basement membrane lesions, showing a positive correlation with proteinuria concentration.
Spontaneous synthesis of colloidal molecules through polymer self-assembly
Scientists developed a novel method to create colloidal molecules with specific symmetry using fluorescent polymers and self-assembly. The process allows for the formation of soft materials with various symmetries depending on the polymer mixing ratio.
Turning up the signal
Osaka University researchers develop a new method for long-range enhancement of fluorescence and Raman signals using Ag nanoislands protected with column-structured silica layers. This leads to an astonishing ten-million-fold increase in signal strength, making it ideal for sensitive biosensing applications.
Researchers can measure distances in molecules optically
Scientists have developed MINFLUX microscopy to measure distances within biomolecules, down to one nanometer, and with Ångström precision. This allows for the detection of different conformations of individual proteins and the observation of their interactions.
2-billion-year-old rock home to living microbes
Researchers have discovered living microbes in a 2-billion-year-old rock sample from the Bushveld Igneous Complex in South Africa. The team used advanced imaging techniques to confirm the presence of indigenous microorganisms, shedding light on the early evolution of life on Earth and the potential for similar organisms to exist on Mars.
Fast and accurate virus detection method using 3D printed setup
A new method combines confocal fluorescence microscopy with microfluidic laminar flow to detect nanoparticles and viruses quickly and accurately. The approach uses a 3D-printed Brick-MIC setup for sensitivity and specificity improvements, potentially changing virus detection in clinical settings.
Deep design produces “butterfly” phase mask for light-sheet fluorescence microscopy
Researchers introduced a novel illumination beam design based on deep learning, eliminating the need for sophisticated optics tools. The approach enhances image quality by optimizing both the deep learning network and the illumination beam simultaneously.
Tracking depression
A team led by Weiying Lin created a molecular probe that selectively detects serotonin, a key player in depression. The study suggests that the ability of neurons to release serotonin is more critical than serotonin levels themselves.
New approach for profiling complex dynamics at the single-molecule level
Researchers developed MUSCLE, a method that combines single-molecule fluorescence microscopy with next-generation sequencing to profile complex biological processes. The technique enables simultaneous observation of vast arrays of samples, uncovering general trends and dynamic signatures.