Articles tagged with Fluorescence Microscopy
Researchers at Salk Institute develop novel 'dye-cycling' strategy to measure RNA polymerase movement along DNA with unprecedented lengths of time. This breakthrough provides critical mechanical insights into how genes are transcribed in cells, shedding light on the fundamental processes of life.
Researchers distinguish multiple fluorescent proteins with overlapping emission colors by their lifetime using fluorescence lifetime imaging microscopy (FLIM). FLIM successfully distinguishes several fluorescent proteins with overlapping color emissions within plant cells, enabling simultaneous analysis of multiple proteins.
Scientists have developed a non-invasive method to predict gene changes causing lung cancer using fluorescence lifetime imaging microscopy (FLIM). This approach can identify specific genetic mutations, such as EGFR, with high accuracy and speed, preserving limited biopsy material for further analysis.
Researchers developed FLIM Playground to streamline FLIM data analysis, extracting lifetime information from cells and visualizing patterns. The platform reduces the need for multiple software tools and quality checks, increasing efficiency and reproducibility.
The Xi Peng laboratory has developed LargePNet, a novel general-purpose fluorescence image restoration network that aggregates large-view structural information to overcome the loss of global contextual information caused by conventional patch-based training. This results in improved restoration accuracy and large-size image inference ...
Researchers at the University of Göttingen have developed a new method to make biomolecules glow in real-time, eliminating unwanted signals in microscopy. This approach ensures only labelled biological molecules emit fluorescence, making experiments clearer and easier to interpret.
Scientists at the Garvan Institute of Medical Research captured 'housekeeping' immune cells actively attacking and engulfing live melanoma cells. These macrophages patrol the edges of melanoma tumours, steadily engulfing cancer cells and slowing tumour growth. The discovery has big implications for immunotherapy.
Researchers at Max Planck Institute for the Science of Light create a 'map' of glycocalyx by mapping individual sugar structures using super-high-resolution microscopy technology. The results show that the spatial arrangement of sugar structures relates to cell physiological state, providing a structured display to the outside world.
A University of Tokyo team developed a fluorescence imaging method to track short-lived molecular intermediates and their magnetic responses in real time. The approach isolates spin-dependent part of chemistry, revealing how magnetically sensitive intermediates appear and disappear.
Researchers at Kyushu University develop a new tissue-clearing reagent, SeeDB-Live, enabling repeated, reversible, and real-time imaging of living brains at greater depth and clarity. This breakthrough allows scientists to visualize neural activity in living mice and brain slices, offering new insights into brain dynamics and function.
Researchers developed a high-speed photoacoustic microscope to track red blood cells in the mouse brain, allowing for single-cell resolution imaging of microvascular structures. This enables study of cerebral small vessel disease, cognitive impairment and dementia.
A new study reveals that astrocytes actively participate in motor-learning circuit rewiring by eliminating synapses in the striatum. The research identifies MEGF10 as a key molecular mediator of this process, which is regulated by dopamine signaling and neural activity.
A new study proposes a fluorescence-based strategy to track microplastics in real time as they move, transform, and degrade inside biological systems. This approach allows precise control over particle brightness, emission wavelength, size, and shape, enabling the tracking of microplastic life cycles from ingestion to breakdown.
Scientists from The University of Osaka have created two new fluorescent markers, Gachapin and Gachapin-C, that can visualize dynamic cell-to-cell contacts and connections within a single neuron's extensions. These indicators allow for the monitoring of complex patterns of connectivity in various cell types, including neurons.
For the first time, researchers have directly visualized how newly formed cellular organelles leave the endoplasmic reticulum and transition onto microtubule tracks inside living cells. The study reveals that the ER plays an active role in steering intracellular traffic.
Researchers have developed bis-pseudoindoxyls, compact fluorophores with red-shifted absorption and fluorescence suitable for time-resolved bioimaging. The dyes exhibit low cytotoxicity and sufficient aqueous solubility, making them ideal for live-cell imaging.
Researchers from the University of Tennessee at Knoxville developed a new statistical method that improves analysis in single-molecule fluorescence experiments. The method combines theoretical work from mathematics with experimental work from molecular biology, allowing for more accurate and efficient data analysis.
Researchers at Ohio State University developed a new approach to immobilize extracellular vesicles in a way that mimics their interactions with tissues. This allows for the study of these particles and their complex interactions with cells, enabling potential applications in disease detection, drug delivery, and biomarker discovery.
Researchers have discovered a key protein structure in the germ cells of male mice that causes deformations in sperm flagellum leading to infertility. The study used ultrastructure expansion microscopy to visualize the centriole, a tiny cylindrical structure critical for sperm movement.
A team of researchers from IOCB Prague introduces a novel method for labeling molecules with fluorescent dyes, surpassing existing approaches in precision and stability. This enables scientists to track labeled molecules over long periods with high reliability, expanding possibilities for research in biology, chemistry, and medicine.
Researchers developed a probe to visualize lipid breakdown in living cells, revealing differences in breakdown rates among individual droplets. The study found that an enzyme called ATGL drives these variations, which may contribute to abnormal lipid metabolism in liver cancer cells.
Researchers at HUN-REN Szegedi Biológiai Kutatóközpont have developed an AI-powered platform for automated 3D cell culture analysis, enabling high-precision screening of cellular models. The technology removes the limitation of throughput in personalized medicine, allowing for fast and accurate analysis of clinical samples.
Researchers developed a new imaging method using multiphoton microscopy to rapidly identify pancreatic neuroendocrine tumors with high accuracy. Machine learning algorithms achieved 80.6% accuracy, while convolutional neural networks outperformed with accuracies ranging from 90.8% to 96.4%.
Researchers developed a fiber-optic method to track Alzheimer's plaques in freely behaving mice, allowing for real-time monitoring and long-term tracking of pathological changes. The technique uses fluorescent dye to bind specifically to amyloid fibrils and provides a minimally invasive way to study disease progression.
A recent study published in Nature Communications reveals that the nucleus is less dense than the surrounding cytoplasm, despite its rich biomolecular composition. The researchers used light to probe density at microscales and found a consistent nuclear-to-cytoplasmic density ratio across eukaryotes.
Cryo-optical microscopy captures high-resolution, quantitatively accurate snapshots of dynamic cellular processes at precisely selected timepoints. This technique enables the observation of transient biological events with unprecedented temporal accuracy.
A newly developed low-cost, handheld intraoral device combines optical diagnostics and image-guided photodynamic therapy to detect and treat early-stage oral cancer. The device shows promising accuracy and effectiveness in detecting PpIX fluorescence and monitoring treatment in real-time.
A new study maps the internal behavior of soft materials when deformed, revealing localized fracture events and heterogeneous flows. The findings challenge long-standing assumptions and provide valuable insights for improving manufacturing techniques.
Researchers developed a cutting-edge microscope to study coral photosynthesis and health in their natural habitat. The BUMP imaging system provides unprecedented insights into coral reefs, advancing efforts to understand coral bleaching.
Researchers developed a new method for imaging enzyme activity in whole organs with high-resolution 3D mapping. This allowed them to visualize differences in aminopeptidase N activity and the effects of inhibitors in mouse kidneys. The study opens up an unbiased evaluation method for drug development.
A team from Peking University achieved a major breakthrough in imaging 15 cellular structures simultaneously using lipid membrane probes, dual-color spinning-disk confocal microscopy, and deep learning. This method enables real-time, long-term organelle tracking with improved efficiency and reduced phototoxicity.
Researchers developed DNA origami structures that selectively deliver fluorescent imaging agents to pancreatic cancer cells, enabling more accurate cancer imaging and selective chemotherapy delivery. The study also explored the use of origami-folded DNA molecules loaded with chemotherapy drugs for targeted delivery to cancer cells.
A novel cannula delivery system allows repeated, nondisruptive delivery of imaging agents to the mouse brain during long-term multiphoton microscopy. This innovation enhances longitudinal studies on brain function, disease progression, and potential treatments.
A new microscopy technique, SIMIP, combines structured illumination with mid-infrared photothermal detection to achieve high-speed chemical imaging with superior resolution. The method outperforms conventional methods in terms of spatial resolution and chemical contrast.
A new imaging technology has been developed that combines super-resolution imaging with artificial intelligence to reveal subcellular structures and dynamics in living cells. This breakthrough enables scientists to better understand the root causes of diseases, leading to improved treatments.
Conjugated small molecular nanoparticles (CSMNs) have shown promise in near-infrared phototheranostics (NIR PTs) for imaging, therapy, and synergistic treatment. Strategies to improve performances and extend absorption wavelengths are crucial for their clinical translations.
The Chinese Medical Association has released updated guidelines for pediatric mycoplasma pneumoniae infection, emphasizing a multi-faceted diagnostic approach and evidence-based treatment strategies. The guidelines highlight the importance of accurate diagnosis and responsible antibiotic use to combat rising resistance.
Scientists developed a novel solvatochromic fluorescent dye that enables high-precision temperature measurements through changes in fluorescence properties. The researchers achieved exceptional sensitivity and resolution, ideal for bioimaging applications.
Researchers used novel fluorogen imaging techniques to visualize biomolecular condensates, revealing distinct environmental and structural features. The study provides insights into the dynamic behavior of these condensates, which play a crucial role in various diseases.
Researchers at Waseda University develop a new imaging technique that uses neutron activation to transform gold nanoparticles into radioisotopes, enabling long-term tracking of their movement in the body. This breakthrough could lead to more effective cancer treatments and precision monitoring of drug distribution.
Researchers found that pancreatic cancer cells gain a survival edge by carrying copies of critical cancer genes on circular pieces of DNA outside chromosomes. The discovery highlights the importance of targeting extrachromosomal DNA in treating the disease.
A new technique called cycleHCR uses DNA barcodes to track hundreds of RNA and protein molecules in single cells within thick biological samples. This allows researchers to decipher how genes function in different parts of an organism, how they enable development, and how they might be altered in diseases.
Recent developments in fluorescent probes offer improved specificity and sensitivity for detecting hepatocellular carcinoma (HCC). Next-generation probes are being developed to enhance tissue penetration depth and probe specificity, potentially improving surgical outcomes.
Researchers have developed a specialized nanoscale material that illuminates cancer cells under freezing conditions, improving surgical precision. This technology enhances surgeons' ability to detect and remove cancer cells during cryosurgery.
Researchers at POSTECH developed a super-photostable organic dye, PF555, to track proteins in cells over extended periods. This breakthrough enables observation of endocytosis and protein interactions, revealing EGFR's active navigation in its environment.
A new universal photocage modification strategy based on thioketal enables real-time live cell subcellular imaging. The thioketal-based probe SiR-EDT exhibits improved dark stability and can be specifically activated by UV-visible light.
A new hybrid microscope allows scientists to image the full 3D orientation and position of an ensemble of molecules, such as labeled proteins inside cells. This can reveal the real biology hidden from just a position change of a molecule alone.
Researchers developed a novel microscopy technique to study metabolic changes in individual cancer cells at the single-cell level. They found that radiation treatment caused significant metabolic shifts in head and neck squamous cell carcinoma cells, particularly through the activation of HIF-1α.
Researchers developed an AI-powered technology that transforms low-resolution, label-free images into high-resolution, virtually stained ones without fluorescent dyes. This innovation delivers stable and accurate cell visualization, overcoming limitations of traditional imaging methods.
Researchers have developed a new AI method that produces sharp microscopy images throughout a thick biological sample, count cells more accurately, and trace vessels in embryos. The technique doesn't require additional equipment beyond a standard microscope and is more accessible than traditional adaptive optics techniques.
Researchers at UChicago captured complete images of adhesion G protein-coupled receptors, revealing their complex extracellular region's interaction with the transmembrane region. The findings suggest alternative means of activating the receptor without separating the GAIN domain.
The study creates ultra-stable thin-film polariton filters with exceptional angular stability, transmitting up to 98% of light, even at extreme viewing angles. This technology has enormous scientific and economic potential for applications in display technology, sensor technologies, biophotonics, and more.
Researchers at Rice University developed soTILT3D, an innovative imaging platform that enables fast and precise 3D imaging of multiple cellular structures while controlling the extracellular environment. The platform improves upon conventional fluorescence microscopy by reducing background fluorescence and increasing imaging speed.
The inaugural workshop at Rice University's Center for Nanoscale Imaging Sciences brought together leading experts to explore advancements in cutting-edge imaging techniques. The event integrated diverse imaging modalities to uncover new insights into biological and materials systems.
Researchers introduce a new approach for megapixel-scale fluorescence microscopy through complex scattering media, resolving high-resolution images without requiring specialized equipment. This technique efficiently corrects distortions caused by light scattering, enabling clear imaging of dense targets.
Researchers developed an AI-powered model called FastGlioma that can detect residual tumor tissue with high accuracy in 10 seconds. The technology outperformed conventional methods, reducing the risk of missed tumors by nearly 75%. This innovation could change the field of neurosurgery and minimize reliance on radiographic imaging.
Scientists have designed bioluminescent proteins that can produce multiple colors of light for real-time imaging in cellular and animal models. These proteins are small, efficient, highly stable and can be used for non-invasive bioimaging, diagnostics, drug discovery and more.
Researchers developed a new imaging technique using fluorescence-guided surgery to enhance visibility of tumors and nerves during head and neck cancer surgery. The technique uses two near-infrared fluorophores, one for tumors and another for facial nerves, allowing for clear differentiation between cancerous tissues and nerves.
Scientists developed a novel method to create colloidal molecules with specific symmetry using fluorescent polymers and self-assembly. The process allows for the formation of soft materials with various symmetries depending on the polymer mixing ratio.
Researchers developed an AI-based method to analyze kidney lesions in female patients with Alport syndrome, predicting renal prognosis and guiding treatment interventions. The approach uses a modified stain and deep learning to detect basement membrane lesions, showing a positive correlation with proteinuria concentration.