Articles tagged with Fluorescence Microscopy
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Comprehensive examination of tools, techniques, methodologies, and approaches used across scientific disciplines to conduct research, collect data, and analyze results. Encompasses experimental procedures, analytical methods, measurement techniques, instrumentation, imaging technologies, spectroscopic methods, laboratory protocols, observational studies, statistical analysis, computational methods, data visualization, quality control, and methodological innovations. Addresses the practical techniques and theoretical frameworks enabling scientists to investigate phenomena, test hypotheses, gather evidence, ensure reproducibility, and generate reliable knowledge through systematic, rigorous investigation across all areas of scientific inquiry.
Study of abstract structures, patterns, quantities, relationships, and logical reasoning through pure and applied mathematical disciplines. Encompasses algebra, calculus, geometry, topology, number theory, analysis, discrete mathematics, mathematical logic, set theory, probability, statistics, and computational mathematics. Investigates mathematical structures, theorems, proofs, algorithms, functions, equations, and the rigorous logical frameworks underlying quantitative reasoning. Provides the foundational language and tools for all scientific fields, enabling precise description of natural phenomena, modeling of complex systems, and the development of technologies across physics, engineering, computer science, economics, and all quantitative sciences.
Scientists have developed a new method to deliver genetic information to stem cells using nanoparticles coated with a specific polymer, enabling more efficient control over cellular differentiation. This innovation has the potential to improve the efficiency and effectiveness of regenerative medicine treatments.
Researchers have discovered that nuclear pore IDPs form a dynamic barrier that allows essential cellular factors to pass while blocking viruses and pathogens. The team used synthetic biology, multidimensional fluorescence microscopy, and computer-based simulations to study IDPs in living cells.
Researchers used microscopy techniques to study polyfluorene chains and found that intra-chain aggregation causes green emission, which disappears when the chain unfolds. The team also discovered a novel optomechanical force acting on some chains, originating from van der Waals interactions and excitonic coupling.
A new Raman probe, 9CN-JCR, has been developed for detecting multiple enzyme activities in heterogeneous biological tissues. The probe exhibits high sensitivity and multiplexing ability, making it a promising tool for cancer diagnosis and research.
A new study reveals that different species of bacteria colonize specific areas on diatoms, reflecting their metabolic properties. The findings provide insight into the complex interactions between algae and bacteria in marine environments.
Scientists have developed a method to activate protein functions using brief flashes of light, enabling precise control over when and where chemical reactions occur. This technology has potential uses in tissue engineering, regenerative medicine, and understanding biological processes.
Researchers develop a new technique to measure blood attenuation using a fluorophore-coated guidewire, improving the accuracy of near-infrared fluorescence in cardiovascular imaging. The method provides accurate information on vessel walls and outperforms existing correction methods.
Researchers have developed a multidisciplinary approach using a new microscope, artificial intelligence algorithm, and voltage indicators to better measure brain activity. The technique enables the imaging of up to 100 neurons at a time, far surpassing previous limits.
A new technique combines machine learning with short-wave infrared fluorescence imaging to detect precise tumor boundaries with higher accuracy than traditional methods. The approach achieved a remarkable per-pixel classification accuracy of 97.5 percent and demonstrated robustness against changes in imaging conditions.
Researchers have developed a high-speed, 3D gigapixel microscope that stitches together dozens of cameras to capture life in unprecedented detail. The device enables the recording of differences in pitch and depth, allowing scientists to study zebrafish behavior and developmental biology without harming the animals.
Researchers have developed a super-resolution microscope with a spatio-temporal precision of one nanometer per millisecond using the MINFLUX technique. This allows them to observe tiny movements of single proteins, including the stepping motion of kinesin-1 along microtubules while consuming ATP.
Molecular biologist Shixin Liu is recognized for developing cutting-edge biophysical tools to visualize and understand biomolecular machines. His work aims to establish a quantitative input-output relationship between environmental stimuli and gene expression profiles.
Researchers developed temporal compressive super-resolution microscopy (TCSRM) to overcome optical diffraction's spatial resolution restriction. TCSRM achieves high-speed imaging at 1200 frames per second with a spatial resolution of 100 nanometers, enabling observation of fast dynamics in fine structures.
Researchers developed a new method combining pMINFLUX microscopy with graphene energy transfer, enabling axial precision of less than 3 angstroms. This allows for the study of molecular structures and dynamics at the nanoscale, fundamental for understanding cellular biomolecular reactions.
A proof-of-principle study introduces a 'glowscope' - a $50 device that converts smartphones into fluorescence microscopes. The device allows for low-magnification imaging of cells, tissues, and organisms under fluorescent lighting.
Researchers have designed a novel imaging and experimental preparation system to record the activity of the enteric nervous system in mice, providing new insights into the complex processes of digestion and waste elimination. The findings suggest that physical distention of the gut controls how the entire neural network is coordinated.
Scientists develop two-beam ultrafast laser scribing technology to fabricate ultrafine graphene patterns with sub-diffraction feature size. The technique overcomes the diffraction limit barrier, allowing for precise control over patterned structures.
Researchers developed BrightEyes-TTM, an open-source stopwatch to study molecular interactions inside living cells. The platform records the lifetime of fluorescent molecules, providing insights into cellular structure and function.
A new biopsy procedure is developed with a multispectral confocal endomicroscope to aid in lung tissue imaging. The system allows for simultaneous imaging of multiple fluorescent dyes, enabling unique identification and spectral unmixing.
Researchers have developed an innovative amputated human limb model to evaluate molecularly targeted fluorescent probes for human tissues. The model allows for controlled testing of imaging agents with zero risk to patients, improving the accuracy and safety of surgical procedures.
Researchers from Osaka University developed a new fluorescent sensor system to visualize N-cadherin-mediated interactions between living cells. The INCIDER system enables accurate tracking of temporal changes in these interactions, with a fluorescence signal 70 times stronger than existing methods.
A new mesoscopic oblique plane microscopy method captures up to three times more resolvable image points than other similar systems, enabling whole-body volumetric recordings of neuronal activity and blood flow dynamics. The technique allows for single-cell tracking within the complete 3D circulation system for the first time.
Researchers developed a photon-efficient volumetric imaging method, laterally swept light-sheet microscopy (iLSLM), which improves axial resolution and optical sectioning while reducing photobleaching. iLSLM outperforms conventional methods like swept focus light-sheet microscopy in terms of resolution and photon efficiency.
Scientists have created a way to track brain diseases like depression, Alzheimer's, and strokes using fluorescent mouse blood. The method allows for months-long study of blood flow in the brain, providing new insights into disease progression and development.
Researchers from the University of Kassel developed an approach to extend the limits of interferometric topography measurements for optical resolution below small structures. Microsphere assistance enables fast and label-free imaging without requiring extensive sample preparation.
Researchers used live fluorescence imaging experiments to uncover the mechanism behind cell volume regulation, revealing that WNK kinases activate the 'switch' through phase separation. This discovery has implications for human health, particularly in relation to kidney function and salt-sensitive hypertension.
A new genome imaging technique captures the structure of the human genome at unprecedented resolution, revealing how individual genes fold and work. This technique, called Modeling immuno-OligoSTORM (MiOS), combines high-resolution microscopy and advanced computational modeling to provide a detailed picture of gene shape and function.
Researchers propose an optical imaging system for real-time hypoxia imaging in cancer treatment. The technique utilizes protoporphyrin IX to enhance contrast between tumors and healthy tissues, allowing for more effective surgical removal.
Researchers developed a low-cost, simple imaging system using tumor-targeting fluorescent molecules to determine tumor depth. The portable system provides quantitative information about the depth of tumor cells in the body, helping surgeons remove healthy tissue around tumors for better outcomes.
Researchers at Universiteit van Amsterdam use fluorescence microscopy and specialized molecules to study the transition from static to dynamic friction. They find that a slip wave propagates from the edge towards the center of the contact area just before sliding occurs.
Researchers developed a new holographic microscope that can see through the intact skull and image the neural network of a living mouse brain with high resolution. The technology uses a wave correction algorithm to filter out multiple scattered light waves, allowing for sharper images.
Scientists at EPFL have created an intelligent microscope that uses artificial neural networks to automate image acquisition and reduce sample stress. The technique can capture more detailed images of bacterial division and mitochondrial constrictions, leading to a better understanding of these rare biological events.
Researchers developed a novel way to visualize densely packed molecules using expansion microscopy, allowing for the first time their imaging. The technique enables visualization of nanostructures found in neurons and Alzheimer's-linked amyloid beta plaques.
A team of researchers developed a deep learning pipeline to analyze vascular system images of plants with high accuracy. The pipeline can detect vascular bundles, identify specific zones, and perform statistical analysis of traits in different stem internodes. This study has the potential to improve crop resilience and food security.
Researchers developed a novel method to visualize proteins secreted by cells with high resolution, using plasmonic-fluor technology. The FluoroDOT assay is versatile, low-cost and adaptable, providing a more comprehensive look at these proteins.
Researchers discovered that CAMSAP2 proteins utilize phase separation to form an 'aster' structure, which then organizes into a microtubule network. This process is crucial for the formation of specialized cell shapes, such as those found in heart muscle and nerve cells.
Researchers developed a non-invasive ocular imaging method to detect flavoprotein fluorescence in the eye, indicating mitochondrial oxidative stress. This technique may predict glaucoma progression earlier than current methods, with similar sensitivity to visual field changes.
Researchers developed a mathematical model to predict the efficiency of nanoparticle delivery into cells, particularly in stem cells. They found that nanoparticles become trapped in bubble-like vesicles, preventing them from reaching their targets.
Researchers developed a novel frequency-domain method to selectively suppress background noise in STED microscopy, achieving higher spatial resolution and improved signal-to-noise ratio. The approach has potential applications in various dual-beam point-scanning techniques.
Researchers will develop and test new beacon molecules and imaging equipment to improve the detection and removal of non-small-cell lung cancers, aiming to reduce unnecessary tissue removal. The goal is to enhance surgical outcomes and improve patient care.
A research team developed a novel super-resolution microscopy technique combining metal-induced energy transfer and single-molecule localization microscopy. The method achieves isotropic three-dimensional imaging of sub-cellular structures, allowing for high-resolution analysis of protein complexes and organelles.
The Biofinder instrument has successfully detected bio-residue in ancient fish fossils from the Green River formation, confirming that biological residues can survive millions of years. The device's capabilities make it an ideal tool for future NASA missions to detect signs of past life on other planetary bodies.
Researchers at Stowers Institute for Medical Research have developed a precise model for the stinging organelle of the starlet sea anemone, revealing its complex architecture and firing mechanism. The findings could lead to beneficial applications in medicine, including microscopic therapeutic delivery devices.
Researchers used fluorescence microscopy to study clathrin-mediated endocytosis in living cells. They found evidence of three models of curvature initiation and discovered that short-lived events favored the constant-curvature model, while longer events preferred the flat-to-curved transition pathway.
A team of researchers at Georgia Tech has developed a custom-built microscope that can reconstruct comprehensive 3D representations with a single camera image. This allows for quantitative analysis of organoids and provides insights into tissue development, drug interaction, and cellular behavior.
A team of researchers has combined expansion microscopy and stimulated Raman scattering microscopy to create a new imaging technique called MAGNIFIERS. This allows for the high-resolution imaging of biomolecules, including proteins, lipids, and DNA, at the nanoscale.
A team of researchers has developed a novel method using infrared imaging to assess glymphatic function, which is crucial for understanding neurological conditions. The technique allows for the measurement of temporal dynamics of glymphatic functions and provides insights into brain fluid exchange and clearance.
A new measurement and imaging approach resolves nanostructures smaller than the diffraction limit without dyes or labels, using polarization and angle-resolved images of transmitted light. The method measures particle size and position with high accuracy, closing the gap between conventional microscopes and super-resolution techniques.
Researchers developed a novel algorithm, 'Joint Space and Frequency Reconstruction' (JSFR-SIM), to accelerate image reconstruction in optically sectioned superresolution structured illumination microscopy. The method achieves 80 times faster execution speed without compromising image quality.
Researchers at Martin Luther University Halle-Wittenberg create a new shape-stabilized phase change material that can absorb significantly more heat and is made of harmless substances. The material, which can be used as large panels integrated into walls, can store up to 24 times more heat than conventional concrete or wallboard.
A new technology called MediSCAPE has been developed by Columbia Engineers that can capture real-time cellular detail in living tissues. This allows doctors to make informed decisions about tumor removal without needing to remove tissue and wait for pathology results.
Researchers at the University of Illinois created quantum dots to visualize macrophages in fat tissue, shedding light on chronic inflammation's role in diseases. The new technology enables accurate cell counting and tracking over time, offering a potential diagnostic tool for insulin resistance and metabolic syndrome.
The researchers discovered two modes of transport that influence whether and how proteins attach themselves to a surface. The team found that rougher surfaces promote longer flights, while less hydrophobic surfaces facilitate quicker localized adsorption/desorption.
The new device, Bio-FlatScope, uses a custom algorithm to reconstruct images of micron-scale targets like cells and blood vessels inside the body. The light captured by Bio-FlatScope can be refocused after the fact to reveal 3D details, making it potentially valuable for detecting cancer or sepsis.
A new fluorescent DNA label has been developed to visualize disrupted DNA architecture in cancer cells, with promising results for improved cancer diagnoses and risk stratification. The study showed that the label can distinguish normal tissue from precancerous and cancerous lesions.
Researchers discovered that Chaoborus larvae adjust their air-sacs' volume by changing the pH level, utilizing resilin's elastic properties. This unusual adaptation enables them to float neutrally buoyant in water.
A team of researchers at Rice University has developed a new method to detect tiny cracks in concrete using silicon fluorescence. The technique involves applying a thin coat of opaque paint to the concrete and shining near-infrared light on it, revealing even the smallest microcracks.
The new BD CellView Image Technology enables high-speed sorting of individual cells based on detailed microscopic analysis, accelerating discovery research in immunology, cell biology, and genomics. This technology has the potential to unlock new cell-based therapeutic discoveries and transform various fields of biomedical research.
A new method combines computational ghost imaging and x-ray fluorescence to create high-resolution chemical element maps. This approach eliminates lenses, reducing scanning time and improving spatial resolution, making it useful for biomedicine, materials science, art analysis, and industrial inspection.
A team from the University of Washington has developed a non-destructive 3D imaging method that can help doctors more accurately diagnose borderline cases of prostate cancer. The new approach uses 3D images to identify complex features in tissue samples, which can increase the likelihood of correctly predicting a cancer's aggressiveness.