Articles tagged with Fluorescence Microscopy
Osaka University researchers develop a new method for long-range enhancement of fluorescence and Raman signals using Ag nanoislands protected with column-structured silica layers. This leads to an astonishing ten-million-fold increase in signal strength, making it ideal for sensitive biosensing applications.
Scientists have developed MINFLUX microscopy to measure distances within biomolecules, down to one nanometer, and with Ångström precision. This allows for the detection of different conformations of individual proteins and the observation of their interactions.
Researchers have discovered living microbes in a 2-billion-year-old rock sample from the Bushveld Igneous Complex in South Africa. The team used advanced imaging techniques to confirm the presence of indigenous microorganisms, shedding light on the early evolution of life on Earth and the potential for similar organisms to exist on Mars.
A new method combines confocal fluorescence microscopy with microfluidic laminar flow to detect nanoparticles and viruses quickly and accurately. The approach uses a 3D-printed Brick-MIC setup for sensitivity and specificity improvements, potentially changing virus detection in clinical settings.
Researchers introduced a novel illumination beam design based on deep learning, eliminating the need for sophisticated optics tools. The approach enhances image quality by optimizing both the deep learning network and the illumination beam simultaneously.
A team led by Weiying Lin created a molecular probe that selectively detects serotonin, a key player in depression. The study suggests that the ability of neurons to release serotonin is more critical than serotonin levels themselves.
Researchers developed a new label-free photothermal microscopy technique using microtoroid optical resonators to detect single nanoparticles. The system achieved high sensitivity and discrimination capabilities, outperforming traditional fluorescence-based methods.
Researchers developed MUSCLE, a method that combines single-molecule fluorescence microscopy with next-generation sequencing to profile complex biological processes. The technique enables simultaneous observation of vast arrays of samples, uncovering general trends and dynamic signatures.
A recent study by Harvard University researchers compares the effectiveness of one-photon (1P) versus two-photon (2P) voltage imaging in neural circuits. The study found that 2P excitation requires approximately 10,000 times more illumination power per cell compared to 1P excitation, posing significant challenges for 2P voltage imaging.
A study published in Neuron reveals that neurons are wired to connect seemingly unrelated concepts, enhancing the brain's ability to predict what we see based on past experiences. Visual experience influences the organisation of feedback projections, which store information about the world.
ProDOL, a novel microscopy technique, enables precise quantification of labelled proteins in living cells, overcoming existing limitations. The method's accuracy and versatility make it a valuable tool for biomedical research, particularly in understanding cellular signalling processes.
A new fluorescence detection system can detect fluorescent proteins from bacteria in water down to levels of less than one part per billion, meeting the World Health Organization’s criteria for detecting fecal contamination. The lensless fluorometer reduces device cost, size and weight while providing better performance.
Researchers have developed a novel deconvolution method called multi-resolution analysis (MRA) that improves image quality without introducing artifacts, allowing for high-fidelity imaging of cells and their processes. The approach capitalizes on the physical properties of excited fluorophores to distinguish useful signals from noise.
A new type of fluorescence microscope has been developed with a resolution better than five nanometres, enabling the capture of even the tiniest cell structures. This breakthrough allows researchers to visualize fine tubes in cells that are only around seven nanometres wide.
Researchers have developed an advanced SRS 3D microscopy called phase-controlled SRS (PC-SRS) for rapid and deep tissue 3D chemical imaging. PC-SRS enables high signal-to-noise ratio, high-speed imaging, and deeper imaging capabilities in highly scattering media.
Researchers developed multiplexed stimulated emission depletion nanoscopy (mSTED) for multi-color live-cell long-term imaging, overcoming limitations of conventional methods. mSTED achieved 5-color imaging with limited photobleaching and phototoxicity, revealing complex interactions between subcellular structures.
Researchers have determined the structure of molecules within an Alzheimer's disease brain for the first time using cryo-electron tomography and fluorescence microscopy. This study revealed the molecular structure of tau protein and its arrangement with amyloid plaques, providing new insights into the pathology of the disease.
The Marine Biological Laboratory has introduced two new microscopes for biological and biomedical research, providing a valuable resource for scientists and students. The instruments enable correlative imaging, allowing researchers to confirm results in different ways, and are expected to influence further development of advanced imagi...
A new deep-physics-informed sparsity framework significantly enhances structural fidelity and universality in fluorescence microscopy. It integrates physical imaging models, prior knowledge, and deep learning to resolve finer details and outperform existing methods.
Researchers at St. Jude Children's Research Hospital have developed a way to mitigate long-lived triplet dark states in smFRET, significantly increasing the method's resolution for molecular imaging. This advancement enables direct visualization of biomolecules' functions and dynamics, crucial for understanding biological processes and...
Researchers at HHMI's Janelia Research Campus have adapted a phase diversity method from astronomy to microscopy, generating clearer images of thick biological samples. The new method is faster and cheaper to implement than current techniques, making adaptive optics more accessible to biologists.
Researchers have developed a highly sensitive diamond quantum magnetometer that can achieve practical ambient condition magnetoencephalography. The novel magnetometer uses a single crystalline diamond to detect magnetic fields, achieving record sensitivities of up to 9.4 pT Hz-1/2 in the frequency range of 5 to 100 Hz.
Researchers at New York University create a new method to see inside crystals, revealing the position of every unit and creating dynamic three-dimensional models. This technique allows scientists to study crystals' chemical history and form, paving the way for better crystal growth and photonic materials.
The team created finely detailed images of the viral RNA and replication structures, revealing spherical shapes around the nucleus of infected cells. The images provide insight into how the virus evades cell defenses and could lead to new therapeutic targets for drug development.
A University of Houston researcher has developed a new method to detect cancer using PANORAMA imaging and fluorescent imaging, achieving a 98.7% accuracy rate. The method analyzes the number and cargo of small EVs in patients' blood samples, allowing for early detection and improved treatment efficacy.
A team of visionaries at the Carney Institute developed 3D-printed brain and spinal cord implants, revolutionizing surgical implantations and optical access. Bioluminescence imaging overcomes limitations of traditional fluorescent microscopy, providing unprecedented observation of neural and vascular activity.
Researchers have created a new technique to engineer dozens of 'FRETfluor' tags, enabling multiplexing of single-molecule measurements. The technique uses advanced chemical building blocks to create a more nuanced spectrum of colors, allowing for the detection of multiple molecules simultaneously.
A new imaging technique developed by researchers at Washington University in St. Louis has allowed scientists to visualize the differences between synthetic peptides and amyloid beta fibril assemblies. The study provides valuable information on the heterogeneity of these assemblies, which is crucial for understanding protein toxicity a...
A new AI model generates realistic images of single cells, which are used as synthetic data to train an AI model for better cell segmentation. The researchers found that providing a more diverse dataset during training improves performance.
Researchers at CeMM Research Center create 'vpCells' method for simultaneous fluorescent labelling of many proteins, enabling precise tracking and exploration of protein function. The approach opens up new applications in fundamental cell biology and drug discovery.
A team at the University of Tokyo has constructed an improved mid-infrared microscope that enables them to see the structures inside living bacteria at the nanometer scale with a resolution of 120 nanometers. This breakthrough can aid multiple fields of research, including into infectious diseases.
Scientists use microprisms to track neuronal activity over multiple days with high resolution and throughput, gaining insights into how the brain adapts and changes over time.
Researchers developed a custom-built, low-cost mesoscope that can adapt to different neuroimaging experiments in live mice and rats. The system offers excellent spatial and temporal resolutions, achieved through its reversible tandem lens configuration, which enables flexible experimentation.
Researchers developed a new catheter-based device combining FLIM with polarization-sensitive OCT to image atherosclerotic plaques. The hybrid approach provides unprecedented information on plaque morphology, microstructure, and biochemical composition.
A new method for phase-modulated stimulated Raman scattering tomography enables rapid, label-free 3D chemical imaging of live cells and tissues. This technique improves lateral resolution and imaging depth compared to conventional methods.
Researchers have created fluorescent, colour-changing dyes that can visualise multiple distinct biological environments using only one dye. These dyes enable 'time travel' within cells by allowing scientists to distinguish between cellular and delivery vessel environments in real-time. The breakthrough has significant implications for ...
Researchers at LMU developed pMINFLUX multiplexing to overcome traditional resolution limits in super-resolution microscopy. This allows for simultaneous localization of multiple dyes and investigation of rapid dynamic processes between biomolecules.
Researchers developed a compact microscope using a single photon avalanche diode array detector, enabling super-resolution imaging with improved signal-to-noise ratio and spatial resolution. The system also combines fluorescence lifetime measurements for enhanced structural specificity.
Researchers have identified a network of lymphatic vessels at the back of the nose as a major hub for CSF outflow to deep cervical lymph nodes. This discovery has significant implications for understanding and treating conditions related to impaired CSF drainage, such as Alzheimer's disease.
A team of researchers has devised a method to deliver mRNA into the brain using lipid nanoparticles, offering new hope for treating conditions like Alzheimer's disease and seizures. The approach uses a special keycard-like system to bypass the blood-brain barrier, allowing therapeutic agents to enter the brain and target specific cells.
A new microscopy technique has been developed to investigate neutral lipids within lipid droplets of living cells. This method allows researchers to monitor the synthesis of neutral lipids directly and observe their behavior over a long period.
MIT researchers have developed a new method to track cell differentiation and study long-term processes like cancer progression or embryonic development. They used noninvasive Raman spectroscopy to monitor embryonic stem cells as they differentiated into multiple cell types over several days.
Researchers develop a versatile imaging system for targeted spectroscopy in the eye fundus, allowing for continuous color imaging and spectral measurements. The system enables users to select targets and move them to any location within the eye fundus region without realignment or fixation changes.
The Janelia Fluor dyes have become a staple in biology labs worldwide, and the team has now expanded their spectrum with a new set of far-red shifted dyes that can penetrate deeper into tissue. The researchers developed a novel chemistry to synthesize these dyes, enabling them to create dozens of functional versions relatively quickly.
Researchers visualized the 3D structure of ASC speck inside cells using advanced fluorescence microscopy methods. The study reveals an amorphous structure with a dense core and filaments extending into the periphery. This breakthrough provides crucial insights for understanding inflammation and immune-related diseases.
Researchers create new methods to visualize and understand samples with increased accuracy and sensitivity. The development of photothermal microscopy, also known as VIP microscopy, enables scientists to probe specific chemical bonds in a specimen, allowing them to map molecules at low concentrations without dye labeling.
A new study published in Cell Reports mapped ketamine's effects on the brains of mice, revealing widespread structural changes in the dopamine system after repeated use. The findings suggest that targeting specific areas of the brain with ketamine therapy could minimize unintended effects.
Researchers have developed PicoRulers, biocompatible molecular rulers for high-resolution microscopy. Using genetic code expansion and click chemistry, the team constructed customized molecular rulers based on the protein PCNA, enabling precise testing of super-resolution microscopy methods on cellular biomolecules.
Researchers at MIT have developed an alternative method to study molecular signals in cells, allowing them to track up to seven different molecules simultaneously. The technique uses fluorescent proteins that flicker on and off at different rates, enabling the tracking of specific cellular functions over time.
Researchers have created two new protocols using novel actinometers to quantify photons, providing versatile and precise light intensity measurements. The protocols are faster, more sensitive, and compatible with imaging systems, enabling accurate measurement of light intensity in biological samples.
A team of researchers at Friedrich-Alexander-Universität Erlangen-Nürnberg has combined artemisinin with coumarin to develop an autofluorescent compound that destroys certain malaria pathogens. The new compound is effective against drug-resistant strains and shows promise for treating malaria.
Researchers developed CL-iSCAT Microscope to visualize cargo trafficking in living cells, revealing traffic jams and collective migration. The technology enables real-time observation of millions of cargos, deepening understanding of cellular biology and potential medical discovery.
Researchers at Auburn University have discovered a specific pathway regulating how older proteins are transported to the cell body for recycling. This process is essential for maintaining effective neural communication and ensuring optimal cognitive function.
Researchers developed an imaging sensor capable of detecting UV light, using it to differentiate between cancer cells and normal cells with 99% confidence. The technology leverages the unique tiered structure of butterfly photoreceptors and perovskite nanocrystals.
Researchers at the University of Würzburg developed a new method to precisely analyze infection pathways of dangerous virus variants using 'clickable' pseudoviruses. These harmless impostors retain their activity and are highly fluorescent, allowing for better visualization of viral infections in living organisms.
A new deblurring algorithm has been developed to improve the resolution of microscopy images without amplifying noise. This breakthrough technique, called 'deblurring by pixel reassignment,' uses local gradients to sharpen images while preserving larger structures.
Cells employ a protective mechanism to preserve orphan ribosomal proteins during heat shock, allowing for rapid recovery once the stress subsides. This study uses lattice light sheet 4D imaging and pulse labeling with HaloTag dye to visualize these processes in real-time.
A team of researchers has developed a new visualization tool combining high-speed cameras and fluorescent injection to distinguish tumor tissue from normal tissue across cancer types. The technique, known as fluorescence lifetime (FLT) imaging, achieved an accuracy of over 97% in distinguishing tumor tissue from healthy tissue.
Heart cells in developing zebrafish transition from silence to beating in a simultaneous, coordinated manner. Each cell can beat independently, and the heartbeat starts from different locations in different zebrafish.
Researchers have identified essential genes for the growth of Patescibacteria, a group of tiny microbes that live on larger bacteria. The study provides insights into their unique biology and potential biotechnology applications.