Articles tagged with Fluorescence Microscopy
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Scientists have created a new imaging method that can detect microscopic soft tissue damage in animal spines, which may lead to improved treatments for lower back pain. The technique uses fluorescent molecules to target denatured collagen and produce precise 3D maps of spinal damage.
A SUTD-led study develops brighter, more sensitive fluorophores by suppressing twisted intramolecular charge transfer (TICT) and enhancing photon-induced electron transfer (PET). The research provides design guidelines for dye chemists to rationally tune TICT, PET, and other mechanisms for a wide range of applications.
Researchers have used advanced microscopy to study the ultrastructure of huntingtin inclusions, revealing different mechanisms of aggregation that lead to distinct biochemical properties. The findings suggest targeting inclusion growth as a potential therapeutic strategy for slowing Huntington's disease progression.
Researchers at the University of Illinois created a novel device using microscopic fluorescent diamonds to calibrate sensitive microscopy systems. The nanodiamonds' stability and longevity make them ideal as a 'first-aid kit' for microscopes, allowing for easy reuse and quality control.
A Harvard research team has created a new method of storing digital information using mixtures of fluorescent dyes, which can potentially store data for thousands of years or more. The technique uses inkjet printing and fluorescence microscopy to encode and decode binary messages in the dye molecules.
Researchers have developed a novel data storage method using mixtures of fluorescent dyes, which can store binary information at high density with fast read/write speeds. The technique encodes sequences of 0s and 1s into dye molecules, allowing for the storage of digital information for thousands of years or longer.
A new study from the University of Gothenburg introduces an AI-based method to develop faster, cheaper, and more reliable information about cells using microscopy. This approach eliminates the drawbacks of traditional fluorescence microscopy by providing accurate results without damaging cells or inhibiting processes.
Researchers developed a cost-effective protocol for plant sample preparation and visualization, eliminating the need for stains and dyes. The new method harnesses the natural autofluorescence of tissues in plants, allowing for rapid visualization of plant anatomy across diverse taxa.
Researchers at Texas Biomedical Research Institute developed 'reporter viruses' that allow for real-time tracking of SARS-CoV-2 spread in cells and animal models. This enables faster screening of potential anti-viral drugs, vaccines, and neutralizing antibodies.
Researchers at Umeå University developed a method to study specific cell types in human organs with high-resolution 3D imaging. This allows for the visualization of previously unrecognized alterations in organs like the pancreas, which can lead to improved understanding of disease conditions and treatment options.
Researchers from Nara Institute of Science and Technology developed a machine learning program that accurately predicts the location of proteins related to actin in cells. The program achieved a high degree of similarity with actual images, showing promise for future applications in cell analysis and artificial cell staining.
Researchers developed a topical fluorescent imaging agent targeting PARP1 enzyme in cervical cancer cells, enabling real-time detection with handheld microscopes. This non-invasive method could revolutionize cervical cancer screenings and biopsies, especially in low-resource areas.
Researchers developed a fluorescent probe that binds to activated platelets, enabling clinicians to proactively treat patients before clotting or scarring occurs. The tool uses intravascular catheter-based imaging and has the potential to optimize patient outcomes.
Researchers have developed a new fluorescence microscopy technique that allows for high-resolution images of microcirculation in the brain without invasive surgical methods. This breakthrough has the potential to reveal new insights into neurological disorders and facilitate early detection and treatment.
Researchers have developed a new technique called diffuse optical localization imaging (DOLI) that enables noninvasive imaging of the brain's microvasculature and neural activity at depths of up to 4 millimeters. This method uses the NIR-II window and is poised to bring new insight into how the brain works in health and disease.
Researchers have developed a novel super-resolution vibrational microscopy harnessing Stimulated Raman Excited Fluorescence (SREF) for ultrasensitive vibrational contrast. This technique enables all-far-field Raman spectroscopy with sensitivity down to single-molecule resolution.
Researchers developed a miniature light-sheet generator that can be implanted into a living animal's brain, enabling high-speed and high-contrast imaging of brain activity. The technology uses nanophotonic technology to create ultrathin silicon-based photonic neural probes that emit multiple addressable thin sheets of light.
A team of researchers from Shanghai Jiao Tong University has developed a new way to break the Abbe diffraction limit and realize subwavelength imaging in an all-optical manner. By utilizing nonlinear four-wave mixing, they create super-resolution through scattering of evanescent fields into the far field.
A new recurrent neural network framework enables fast and efficient 3D imaging of fluorescent samples, reducing scan times by ~30-fold. The approach uses few 2D images to reconstruct 3D images, mitigating photo-bleaching challenges in live sample experiments.
Researchers at FAU have uncovered the secret behind the Pazyryk carpet's vivid colors using high-resolution x-ray fluorescence microscopy. Fermenting sheep's wool before dyeing increases brilliance and longevity of the color.
Fermented wool retains its color without fading, a technique used by textile craftsmen in the Iron Age. The method involves fermenting wool and dyeing it with Turkey red, providing an insight into ancient textile production.
Researchers discovered that photobleaching can transform fluorescent dyes into new molecules with altered fluorescence spectra, affecting microscopy results. Simple buffer additions can prevent or even exploit this effect for targeted tracking of specific particles.
Researchers at Heidelberg University developed a novel fluorescence marker called RhoBAST to enable super-resolution RNA imaging in live cells. The method reveals details of subcellular structures and molecular interactions involving RNA, improving image resolution.
Scientists are gaining a deeper understanding of extracellular vesicles (EVs), tiny particles that carry unique cargo from cells. EVs play a critical role in communication between cells, contributing to conditions like cancer and neurodegenerative diseases.
A new scanless high-speed holographic fluorescence microscopy system has been developed with submicron resolution, enabling 3D sensing of nanoparticles and color-multiplexed holographic fluorescence imaging. The system achieves measurements in less than 1 millisecond using digital holography and a phase modulator.
Scientists from Japan develop a novel approach to acquire fluorescence lifetime images without scanning, using optical frequency combs and high-speed single-point photodetectors. This method offers superior speed and high spatial resolution for simultaneous imaging of multiple samples.
Researchers have developed a novel synthetic aperture microscopy method using digital micromirror devices, achieving high spatial resolution and fast imaging speeds. The technique enables the observation of subcellular dynamics and nanometric structures without harming living cells.
Holographic fluorescence imaging combines sensitivity, resolution, and specificity to track individual particles in 3D. The technique uses lateral shearing-interferometry to access phase information of each photon, enabling single-molecule sensitivity.
Researchers from Kyushu University developed a technique that improves the resolution of fluorescence images of living cells using plasmonic metasurfaces. The metasurface, composed of self-assembled gold nanoparticles, enhances the focus of light-emitting molecules, resulting in high-resolution imaging capabilities.
Researchers have successfully raised funding for a project to develop a new super-resolution microscopy technique that can visualize individual synapse proteins with nanometer-scale resolution. The project combines expansion microscopy and single-molecule-sensitive super-resolution microscopy to achieve improved microscopic resolution.
The new technique allows for simultaneous acquisition of images at different depths using a standard microscope, improving biological imaging applications. It utilizes a z-splitter prism to divide detected light, producing multiple high-resolution images on the same sensor without overlap.
A Polish-Israeli team has introduced a new method of super-resolution microscopy that, in theory, has no resolution limit. The technique, called SOFISM, uses naturally occurring fluctuations in emission intensity to enhance spatial resolution.
Researchers at KAUST developed a cost-effective, ultrathin SRS lens using laser-based 3D printing, inspired by lighthouse design. The new lens rejects cross-phase modulation background signals, improving imaging efficiency for biological processes like cancer cell growth.
A new method for identifying sentinel lymph nodes (SLNs) in breast cancer uses photoacoustic microscopy and CD44 and SR-B1 dual-targeting nanoparticles. The technique distinguishes between metastatic SLNs and inflamed LNs, providing a potential solution for reducing complications during surgery.
Researchers from Osaka City University developed a microscope-based thermometer that uses quantum technology to detect temperature changes in live, microscopic animals. The thermometry algorithm successfully tracked temperature fluctuations in C. elegans nematode worms after inducing a fever by stimulating their mitochondria.
Researchers have developed a new method to analyze microscopic samples without using external light, reducing interference and damage to living specimens. The 'glow in the dark' approach uses chemical stimuli to activate chemicals, enabling precise control over localized oxidative hotspots.
Researchers have overcome the limitation of super-resolution microscopy by combining dSTORM and expansion microscopy, achieving a distance error reduction to just five nanometers. This enables fluorescence imaging with molecular resolution for the first time, allowing detailed insights into molecular function and architecture.
Researchers have developed new techniques that can significantly reduce the time needed to process complex images from cutting-edge microscopes. These methods use deconvolution algorithm modifications, parallelization, and neural networks to speed up processing time by several thousand-fold.
A new microscopy technique has pinpointed the locations of individual proteins within bacterial cells, revealing their precise positions and interactions. The technique, called CIASM, combines fluorescent imaging with cryogenic electron tomography to produce high-resolution images of molecules in their cellular neighborhoods.
Researchers developed a single-molecule orientation imaging approach to study amyloid proteins, revealing nanoscale differences in their structures. The method provides insights into the fundamental biological mechanisms of disease, potentially contributing to the development of effective therapeutics.
Researchers created a multimodal digital holographic microscope that can produce 3D fluorescence and phase images of living cells without scanning. This technology has the potential to increase our understanding of stem cell processes in plants and revolutionize biology.
Researchers have developed genetically-encoded X-ray-sensitive tags for site-specific labeling of protein-of-interest in mammalian cells. This enables endogenous labeling of diverse molecules and subcellular structures with an ultrahigh spatial resolution of ~30 nm. The high photostability of these tags allows long-term observation of ...
Researchers at UNSW Sydney have developed a new imaging technique that uses the color of cells as a 'thermometer' for molecular imbalance. This technology has the potential to revolutionize medical diagnostics by allowing scientists to detect and decode cell colors without needing to extract samples from the body.
Researchers discovered a potential new method to detect age-related macular degeneration using tetracycline staining and fluorescence lifetime imaging microscopy. Tiny deposits of lipids, proteins, and minerals under the retina can be visualized with this technique, offering enhanced early detection.
Researchers at UNSW achieved unprecedented resolution capabilities in single-molecule microscopy to detect interactions between individual molecules within intact cells. Their self-aligning microscope smashed the limits of existing super-resolution microscopy technology by measuring distances between proteins with nanometre precision.
Researchers have developed a new method to visualize fungi using expansion microscopy, allowing for detailed studies of fungal biology and potential applications in medicine. The technique has been successfully applied to three fungal species, including the clinically relevant Aspergillus fumigatus.
Researchers developed a computer program to identify each nerve cell in fluorescent microscope images of living worms, overcoming previous challenges by creating unique genetic modifications. The program uses a mathematical algorithm to analyze images and assign neuron identities based on position variations between individual animals.
A new form of imaging modality called coded light-sheet array microscopy (CLAM) allows for full 3D parallelized fluorescence imaging without scanning. CLAM reduces photobleaching and preserves biological specimen viability, enabling long-term volumetric imaging.
A new microscopy technique allows researchers to follow individual proteins over long periods of time as they move along and inside live cells. The technique, called interferometric scattering (iSCAT) microscopy, can track proteins with microsecond speeds for extended periods.
A new microscopy technique, cryo-SR/EM, combines images from electron microscopes and super-resolution light microscopes to reveal the intricate 3D structure of cells. This allows researchers to study the relationships between cellular structures and their surroundings with unprecedented clarity.
Researchers used a label-free technique to investigate the metabolism of living biological tissues in fruit flies. They found that sperm had a highly glycolytic metabolism similar to that of cancer cells, which may contribute to their ability to remain fresh in female bodies. The study also suggests potential clinical applications for ...
A Chinese fossil specimen was misidentified as an ancient spider species, with experts at the University of Kansas using fluorescence microscopy to uncover its true nature as a heavily altered crayfish. The study highlights the potential for fossils to be intentionally or unintentionally misrepresented.
A new imaging technology assesses conjunctival goblet cells with high definition and contrast, overcoming limitations of existing methods. This non-invasive approach enables precise diagnosis of dry eye syndromes and evaluation of treatment effects, paving the way for precision medicine.
Researchers from DUT and SUTD developed novel rhodamines to overcome fluorophore brightness limitations in SMLM, enabling clearer resolution and analysis of cellular structures. The new dyes show increased fluorescence brightness and 'photon budget', crucial for high-resolution imaging.
Researchers at Rensselaer Polytechnic Institute developed a new deep neural network to improve fluorescence lifetime imaging, enabling rapid and detailed analysis of cellular interactions in cancer cells. This technique requires less light while producing detailed images, bringing the field closer to clinical use for precision medicine.
Scientists at Tokyo Tech achieve unprecedented precision in localizing biomolecules within intact cells using cryogenic fluorescence microscopy. The technique corrects the 'dipole orientation effect', a major limitation in fluorescence microscopy, resulting in nanometer-level accuracy.
Researchers at University of Tsukuba developed a new CRIF method to detect unique fluorescent signatures of individual microbial cells in mixtures. The non-destructive technique allows for realistic three-dimensional environments and can distinguish between different types of microbes.
A European research team has developed a method to track the HI virus's spread between living cells using superresolution STED fluorescence microscopy. The study reveals that the HIV pathogen creates a specific lipid environment for replication, providing a potential target for antiviral drugs.
Researchers from Bielefeld University have developed a faster method for super-resolution SR-SIM microscopy, allowing for real-time recording of cell movements and observations of small structures. This enables biologists to explore such structures in detail, particularly in the study of viral particles on their way through cells.
A new interferometric single-molecule localization microscopy, called Repetitive Optical Selective Exposure (ROSE), has been developed to improve the precision of nanostructure imaging. ROSE achieves a two-fold improvement in localization precision compared to conventional methods.