Articles tagged with Fluorescence Microscopy
Researchers at UNSW Sydney have developed a new imaging technique that uses the color of cells as a 'thermometer' for molecular imbalance. This technology has the potential to revolutionize medical diagnostics by allowing scientists to detect and decode cell colors without needing to extract samples from the body.
Researchers discovered a potential new method to detect age-related macular degeneration using tetracycline staining and fluorescence lifetime imaging microscopy. Tiny deposits of lipids, proteins, and minerals under the retina can be visualized with this technique, offering enhanced early detection.
Researchers at UNSW achieved unprecedented resolution capabilities in single-molecule microscopy to detect interactions between individual molecules within intact cells. Their self-aligning microscope smashed the limits of existing super-resolution microscopy technology by measuring distances between proteins with nanometre precision.
Researchers have developed a new method to visualize fungi using expansion microscopy, allowing for detailed studies of fungal biology and potential applications in medicine. The technique has been successfully applied to three fungal species, including the clinically relevant Aspergillus fumigatus.
Researchers developed a computer program to identify each nerve cell in fluorescent microscope images of living worms, overcoming previous challenges by creating unique genetic modifications. The program uses a mathematical algorithm to analyze images and assign neuron identities based on position variations between individual animals.
A new form of imaging modality called coded light-sheet array microscopy (CLAM) allows for full 3D parallelized fluorescence imaging without scanning. CLAM reduces photobleaching and preserves biological specimen viability, enabling long-term volumetric imaging.
A new microscopy technique allows researchers to follow individual proteins over long periods of time as they move along and inside live cells. The technique, called interferometric scattering (iSCAT) microscopy, can track proteins with microsecond speeds for extended periods.
A new microscopy technique, cryo-SR/EM, combines images from electron microscopes and super-resolution light microscopes to reveal the intricate 3D structure of cells. This allows researchers to study the relationships between cellular structures and their surroundings with unprecedented clarity.
Researchers used a label-free technique to investigate the metabolism of living biological tissues in fruit flies. They found that sperm had a highly glycolytic metabolism similar to that of cancer cells, which may contribute to their ability to remain fresh in female bodies. The study also suggests potential clinical applications for ...
A Chinese fossil specimen was misidentified as an ancient spider species, with experts at the University of Kansas using fluorescence microscopy to uncover its true nature as a heavily altered crayfish. The study highlights the potential for fossils to be intentionally or unintentionally misrepresented.
A new imaging technology assesses conjunctival goblet cells with high definition and contrast, overcoming limitations of existing methods. This non-invasive approach enables precise diagnosis of dry eye syndromes and evaluation of treatment effects, paving the way for precision medicine.
Researchers from DUT and SUTD developed novel rhodamines to overcome fluorophore brightness limitations in SMLM, enabling clearer resolution and analysis of cellular structures. The new dyes show increased fluorescence brightness and 'photon budget', crucial for high-resolution imaging.
Researchers at Rensselaer Polytechnic Institute developed a new deep neural network to improve fluorescence lifetime imaging, enabling rapid and detailed analysis of cellular interactions in cancer cells. This technique requires less light while producing detailed images, bringing the field closer to clinical use for precision medicine.
Scientists at Tokyo Tech achieve unprecedented precision in localizing biomolecules within intact cells using cryogenic fluorescence microscopy. The technique corrects the 'dipole orientation effect', a major limitation in fluorescence microscopy, resulting in nanometer-level accuracy.
Researchers at University of Tsukuba developed a new CRIF method to detect unique fluorescent signatures of individual microbial cells in mixtures. The non-destructive technique allows for realistic three-dimensional environments and can distinguish between different types of microbes.
A European research team has developed a method to track the HI virus's spread between living cells using superresolution STED fluorescence microscopy. The study reveals that the HIV pathogen creates a specific lipid environment for replication, providing a potential target for antiviral drugs.
Researchers from Bielefeld University have developed a faster method for super-resolution SR-SIM microscopy, allowing for real-time recording of cell movements and observations of small structures. This enables biologists to explore such structures in detail, particularly in the study of viral particles on their way through cells.
A new interferometric single-molecule localization microscopy, called Repetitive Optical Selective Exposure (ROSE), has been developed to improve the precision of nanostructure imaging. ROSE achieves a two-fold improvement in localization precision compared to conventional methods.
Researchers developed a sensitive, portable device that can detect as few as 5-6 norovirus particles per sample, making it suitable for practical applications. The device uses fluorescence to detect norovirus and is compact enough for handheld use, enabling municipal water systems staff to check for the virus in the water supply.
A team of scientists at EPFL developed an algorithm that can estimate a microscope's resolution from a single image, boosting image quality and enabling optimized imaging conditions. The algorithm has been made available as an open-source plugin, allowing researchers to directly obtain the estimate and optimize their microscopes.
Researchers have found that lithium fluoride crystals can detect tracks of heavy ions with high energies, including iron. The crystals work like photographic film and can accurately reproduce the path of a particle.
Researchers successfully recorded sequences of up to 1,000 sharp images of live mitochondria at 1.5 frames per second using STED microscopy. By exploiting the probabilistic nature of photobleaching, scientists can distinguish between different regions of the cell and achieve unprecedented resolution.
Researchers at Rice University have developed a set of eight fluorescent surfactants that can capture images of single nanotubes or cells using fluorescent microscopy. These compounds show promise for use in medicine, manufacturing, water purification and biomedical applications.
Researchers have invented a new type of microscopy called 'DNA microscopy' that can image cells at the genomic level. This technique uses DNA bar codes to pinpoint molecules' relative positions within a sample, allowing scientists to build a picture of cells and amass enormous amounts of genomic information.
Researchers have engineered a new fluorescent protein that glows under UV and blue light, is thermally stable, and can emit light in the absence of oxygen. This breakthrough resolves previous limitations of fluorescence microscopy, enabling scientists to study living tissue more effectively.
A new analytical tool for ultra-trace ratiometric detection of capsaicinoids has been developed, enabling the quick and reliable quantification of spicy tastes in kimchi. The method uses a combined technology of endogenous fluorescence imaging techniques and total internal reflection fluorescence microscopy.
Researchers have developed a technique using time signals 'temporal barcodes' that can label molecules with distinct flashing patterns. This allows for the detection and identification of any number of molecules, including proteins, at the molecular scale, increasing efficiency and reducing costs compared to traditional methods.
Researchers at the University of Houston are developing a new imaging technology that can simultaneously capture structural and molecular changes in embryos during critical periods of development. This breakthrough could lead to improved early detection and prevention of birth defects with long-term chronic conditions.
Researchers have developed a portable mini microscope that allows for real-time brain imaging in living mice, reducing costs and enabling more detailed studies of diseases such as cancer, stroke, and Alzheimer's. The device records images from active brains of mice moving naturally around their environments.
Rice University researchers have developed a method to capture 4D data using 2D microscopes, enabling scientists to visualize molecules' locations and movements in living cells. The technique uses custom phase masks to manipulate light and separate spatial and temporal information.
A 150-year-old fossil feather has been re-examined using Laser-Stimulated Fluorescence (LSF) technology, resolving the debate over its origin. The study reveals that the feather did not belong to Archaeopteryx but instead came from an unknown feathered dinosaur.
Biological imaging experts at Colorado State University have used a custom fluorescence microscope to capture individual RNA molecules interacting with stress granules. The results show that RNA translation is completely silenced before the RNAs enter the stress granules, providing unprecedented details of the cellular stress response.
A team of scientists has captured a high-resolution, three-day image of the fly brain using expansion plus lattice light-sheet microscopy. The new imaging technique allows for rapid 3D images of brain tissue with details as small as 60 nanometers across.
Researchers have developed a new way to image the brain with unprecedented resolution and speed, revealing individual neurons and their connections. The technique combines expansion microscopy with lattice light-sheet microscopy, allowing for rapid imaging of large volumes of brain tissue.
Scientists have developed a new imaging technique that allows for rapid and detailed scanning of entire brains at the nanoscale. This breakthrough method, combined with the lattice light-sheet microscope, enables visualization of any desired protein and has the potential to revolutionize neuroscience research.
Researchers at the University of Geneva have developed a new technique called Ultrastructure Expansion Microscopy (U-ExM), which allows for the visualization of cellular structures and protein complexes at a nanoscale. This method enables the detection of biochemical modifications and mapping of large intracellular molecular complexes.
Researchers at NSLS-II produce 3D images of a single bacterial cell's chemical composition, identifying calcium and zinc distributions. The technique demonstrates high-resolution imaging capabilities for understanding cellular processes and developing medical treatments.
Researchers have developed a new technique that combines light-sheet fluorescence microscopy with three-photon absorption to image deeper into tissue. This breakthrough could improve neuroscience and developmental studies, and may be useful for drug discovery.
Scientists developed a new method to analyze and reconstruct super-resolution images into a 3D volume with multiple colors. This technique enables the observation of complex molecular structures in cells, resolving protein complexes previously invisible.
Researchers developed switchable fluorescent proteins that can be controlled by green and orange light, enabling the study of dynamic processes in living cells without harming them. The proteins' efficient photoswitching allows for super-resolution fluorescence microscopy, a method previously hampered by toxic irradiation.
Scientists developed a machine learning technique to predict human cell organization using only black and white images generated by brightfield microscopy. This allows for the exploration of cellular structures in ways that were previously impossible, particularly in live cells.
PySight improves rapid 2D and 3D imaging of the brain with high spatiotemporal resolution, enabling scientists to better understand brain dynamics and discover new treatments. The open-source software integrates with state-of-the-art hardware, overcoming technical barriers to continuous 3D imaging.
Researchers at the University of Kansas have developed two novel imaging procedures that enable better, more useful digital pictures of Earth's biodiversity. The techniques involve 'cleared and stained' specimens and fluorescent microscopy, allowing scientists to capture intricate anatomical structures in new ways.
Researchers evaluated state-of-the-art optical technology in commercial-grade operating microscopes to detect fluorescence signals produced by pro-drug 5-ALA. They found variability in signal intensity and bleaching rates, highlighting the need for standardized methods and built-in standards for reliable detection and measurement.
Lehigh University's Xiaoji Xu has been awarded a Beckman Young Investigator grant to develop an infrared microscopy technique that surpasses current limitations. The goal is to achieve nanoscale imaging in the aqueous phase, opening up new avenues for chemistry and biology research.
Scientists at Ludwig-Maximilians-Universität München have created novel DNA aptamers that enable the use of smaller fluorescent labels in super-resolution microscopy. This breakthrough allows for high-resolution imaging of protein networks within individual cells, paving the way for new insights into biological processes.
A team of researchers has developed a photostable fluorescent labeling agent for single molecule, multicolor, and 3D deep imaging in the near infrared region. The new dye, PREX 710, allows for long-term bioimaging of blood vessels in mice brains.
Researchers developed a new NIR-II fluorescent molecule for dual fluorescence and photoacoustic imaging, offering high resolution and penetration depth for precise noninvasive brain-tumor diagnosis. The method demonstrated high sensitivity and specificity, accurately assessing tumor location and depth in brain tissue.
Researchers at the University of Münster developed a new substance similar to cholesterol, allowing visualization in living cells. The study enables imaging of membrane dynamics without damaging the membrane.
Juhyun Lee is using a new $251,000 grant from the American Heart Association to explore targeted delivery of messenger RNA to reverse damage caused by congenital heart disease. His goal is to modulate mechanical forces and reduce gene expressions to stimulate heart growth or healing.
Sabine Petry and her team used novel imaging technique to show that XMAP215 works with gamma-tubulin ring complex to create microtubule nuclei. They found that XMAP215 promotes efficient microtubule nucleation, resolving a long-standing puzzle in cell biology.
A team of Harvard scientists discovered that tiny Phoroncidia rubroargentea spiders use a combination of structural colors, pigment, and fluorescent material to produce their distinctive red and silver hues. The colors are stabilized by a tough cuticle layer, with the silver color relying on a reflective material similar to fish scales.
Scientists combined two microscope technologies to create a microscope that offers unparalleled look at biological processes. The new microscope enables observation of rapidly moving objects about 10 times faster than other microscopes at similar resolution.
Dr. Juhyun Lee is using a $154,000 grant from the American Heart Association to develop a microscope that can capture 3-D motion and track gene development in zebrafish. This research could lead to identifying heart abnormalities and diagnosing congenital conditions for potential treatment with gene therapy.
The Rice team designs a thin, wide-field microscope that surpasses traditional microscopes in resolution and field of view. FlatScope eliminates the need for lenses, allowing for micrometer resolution over several cubic millimeters.
A team of researchers has developed a technique that can perform both 3D super-resolution microscopy and fast 3D phase imaging in a single instrument, enabling high-time resolution visualization of living cells. This new platform, called PRISM, allows for direct visualization and analysis of subcellular structures without labeling.
Researchers at Carnegie Mellon University are developing new technologies for understanding the brain, including high-throughput fluorescence synapse quantitation and a confocal fluorescence microscopy data repository. They aim to identify how and where synapses develop and change to understand learning, development, and disease.
A new microscope, Firefly, has been developed to study brain activity and neurological disorders. With a 6-millimeter-diameter field of view, the microscope can image neural circuits containing hundreds of cells, allowing for the observation of electrical pulses traveling between neurons.
Researchers from University of Houston release open-source dataset and instructions for building a smartphone microscope with an inexpensive inkjet-printed elastomer lens. The device can perform fluorescence microscopy, detect waterborne pathogens, and has potential applications in rural areas and developing countries.
Researchers developed a microfluidic chip-based platform for analyzing live cells using fluorescence microscopy. The platform uses a CMOS image sensor and allows for fully automated systems, making it suitable for high-throughput applications.