Articles tagged with Fluorescence Microscopy
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Researchers developed a sensitive, portable device that can detect as few as 5-6 norovirus particles per sample, making it suitable for practical applications. The device uses fluorescence to detect norovirus and is compact enough for handheld use, enabling municipal water systems staff to check for the virus in the water supply.
A team of scientists at EPFL developed an algorithm that can estimate a microscope's resolution from a single image, boosting image quality and enabling optimized imaging conditions. The algorithm has been made available as an open-source plugin, allowing researchers to directly obtain the estimate and optimize their microscopes.
Researchers have found that lithium fluoride crystals can detect tracks of heavy ions with high energies, including iron. The crystals work like photographic film and can accurately reproduce the path of a particle.
Researchers successfully recorded sequences of up to 1,000 sharp images of live mitochondria at 1.5 frames per second using STED microscopy. By exploiting the probabilistic nature of photobleaching, scientists can distinguish between different regions of the cell and achieve unprecedented resolution.
Researchers at Rice University have developed a set of eight fluorescent surfactants that can capture images of single nanotubes or cells using fluorescent microscopy. These compounds show promise for use in medicine, manufacturing, water purification and biomedical applications.
Researchers have invented a new type of microscopy called 'DNA microscopy' that can image cells at the genomic level. This technique uses DNA bar codes to pinpoint molecules' relative positions within a sample, allowing scientists to build a picture of cells and amass enormous amounts of genomic information.
Researchers have engineered a new fluorescent protein that glows under UV and blue light, is thermally stable, and can emit light in the absence of oxygen. This breakthrough resolves previous limitations of fluorescence microscopy, enabling scientists to study living tissue more effectively.
A new analytical tool for ultra-trace ratiometric detection of capsaicinoids has been developed, enabling the quick and reliable quantification of spicy tastes in kimchi. The method uses a combined technology of endogenous fluorescence imaging techniques and total internal reflection fluorescence microscopy.
Researchers have developed a technique using time signals 'temporal barcodes' that can label molecules with distinct flashing patterns. This allows for the detection and identification of any number of molecules, including proteins, at the molecular scale, increasing efficiency and reducing costs compared to traditional methods.
Researchers at the University of Houston are developing a new imaging technology that can simultaneously capture structural and molecular changes in embryos during critical periods of development. This breakthrough could lead to improved early detection and prevention of birth defects with long-term chronic conditions.
Researchers have developed a portable mini microscope that allows for real-time brain imaging in living mice, reducing costs and enabling more detailed studies of diseases such as cancer, stroke, and Alzheimer's. The device records images from active brains of mice moving naturally around their environments.
Rice University researchers have developed a method to capture 4D data using 2D microscopes, enabling scientists to visualize molecules' locations and movements in living cells. The technique uses custom phase masks to manipulate light and separate spatial and temporal information.
A 150-year-old fossil feather has been re-examined using Laser-Stimulated Fluorescence (LSF) technology, resolving the debate over its origin. The study reveals that the feather did not belong to Archaeopteryx but instead came from an unknown feathered dinosaur.
Biological imaging experts at Colorado State University have used a custom fluorescence microscope to capture individual RNA molecules interacting with stress granules. The results show that RNA translation is completely silenced before the RNAs enter the stress granules, providing unprecedented details of the cellular stress response.
Scientists have developed a new imaging technique that allows for rapid and detailed scanning of entire brains at the nanoscale. This breakthrough method, combined with the lattice light-sheet microscope, enables visualization of any desired protein and has the potential to revolutionize neuroscience research.
A team of scientists has captured a high-resolution, three-day image of the fly brain using expansion plus lattice light-sheet microscopy. The new imaging technique allows for rapid 3D images of brain tissue with details as small as 60 nanometers across.
Researchers have developed a new way to image the brain with unprecedented resolution and speed, revealing individual neurons and their connections. The technique combines expansion microscopy with lattice light-sheet microscopy, allowing for rapid imaging of large volumes of brain tissue.
Researchers at the University of Geneva have developed a new technique called Ultrastructure Expansion Microscopy (U-ExM), which allows for the visualization of cellular structures and protein complexes at a nanoscale. This method enables the detection of biochemical modifications and mapping of large intracellular molecular complexes.
Researchers at NSLS-II produce 3D images of a single bacterial cell's chemical composition, identifying calcium and zinc distributions. The technique demonstrates high-resolution imaging capabilities for understanding cellular processes and developing medical treatments.
Researchers have developed a new technique that combines light-sheet fluorescence microscopy with three-photon absorption to image deeper into tissue. This breakthrough could improve neuroscience and developmental studies, and may be useful for drug discovery.
Scientists developed a new method to analyze and reconstruct super-resolution images into a 3D volume with multiple colors. This technique enables the observation of complex molecular structures in cells, resolving protein complexes previously invisible.
Researchers developed switchable fluorescent proteins that can be controlled by green and orange light, enabling the study of dynamic processes in living cells without harming them. The proteins' efficient photoswitching allows for super-resolution fluorescence microscopy, a method previously hampered by toxic irradiation.
Scientists developed a machine learning technique to predict human cell organization using only black and white images generated by brightfield microscopy. This allows for the exploration of cellular structures in ways that were previously impossible, particularly in live cells.
PySight improves rapid 2D and 3D imaging of the brain with high spatiotemporal resolution, enabling scientists to better understand brain dynamics and discover new treatments. The open-source software integrates with state-of-the-art hardware, overcoming technical barriers to continuous 3D imaging.
Researchers at the University of Kansas have developed two novel imaging procedures that enable better, more useful digital pictures of Earth's biodiversity. The techniques involve 'cleared and stained' specimens and fluorescent microscopy, allowing scientists to capture intricate anatomical structures in new ways.
Researchers evaluated state-of-the-art optical technology in commercial-grade operating microscopes to detect fluorescence signals produced by pro-drug 5-ALA. They found variability in signal intensity and bleaching rates, highlighting the need for standardized methods and built-in standards for reliable detection and measurement.
Scientists at Ludwig-Maximilians-Universität München have created novel DNA aptamers that enable the use of smaller fluorescent labels in super-resolution microscopy. This breakthrough allows for high-resolution imaging of protein networks within individual cells, paving the way for new insights into biological processes.
Lehigh University's Xiaoji Xu has been awarded a Beckman Young Investigator grant to develop an infrared microscopy technique that surpasses current limitations. The goal is to achieve nanoscale imaging in the aqueous phase, opening up new avenues for chemistry and biology research.
A team of researchers has developed a photostable fluorescent labeling agent for single molecule, multicolor, and 3D deep imaging in the near infrared region. The new dye, PREX 710, allows for long-term bioimaging of blood vessels in mice brains.
Researchers developed a new NIR-II fluorescent molecule for dual fluorescence and photoacoustic imaging, offering high resolution and penetration depth for precise noninvasive brain-tumor diagnosis. The method demonstrated high sensitivity and specificity, accurately assessing tumor location and depth in brain tissue.
Researchers at the University of Münster developed a new substance similar to cholesterol, allowing visualization in living cells. The study enables imaging of membrane dynamics without damaging the membrane.
Juhyun Lee is using a new $251,000 grant from the American Heart Association to explore targeted delivery of messenger RNA to reverse damage caused by congenital heart disease. His goal is to modulate mechanical forces and reduce gene expressions to stimulate heart growth or healing.
Sabine Petry and her team used novel imaging technique to show that XMAP215 works with gamma-tubulin ring complex to create microtubule nuclei. They found that XMAP215 promotes efficient microtubule nucleation, resolving a long-standing puzzle in cell biology.
A team of Harvard scientists discovered that tiny Phoroncidia rubroargentea spiders use a combination of structural colors, pigment, and fluorescent material to produce their distinctive red and silver hues. The colors are stabilized by a tough cuticle layer, with the silver color relying on a reflective material similar to fish scales.
Scientists combined two microscope technologies to create a microscope that offers unparalleled look at biological processes. The new microscope enables observation of rapidly moving objects about 10 times faster than other microscopes at similar resolution.
Dr. Juhyun Lee is using a $154,000 grant from the American Heart Association to develop a microscope that can capture 3-D motion and track gene development in zebrafish. This research could lead to identifying heart abnormalities and diagnosing congenital conditions for potential treatment with gene therapy.
The Rice team designs a thin, wide-field microscope that surpasses traditional microscopes in resolution and field of view. FlatScope eliminates the need for lenses, allowing for micrometer resolution over several cubic millimeters.
A team of researchers has developed a technique that can perform both 3D super-resolution microscopy and fast 3D phase imaging in a single instrument, enabling high-time resolution visualization of living cells. This new platform, called PRISM, allows for direct visualization and analysis of subcellular structures without labeling.
Researchers at Carnegie Mellon University are developing new technologies for understanding the brain, including high-throughput fluorescence synapse quantitation and a confocal fluorescence microscopy data repository. They aim to identify how and where synapses develop and change to understand learning, development, and disease.
A new microscope, Firefly, has been developed to study brain activity and neurological disorders. With a 6-millimeter-diameter field of view, the microscope can image neural circuits containing hundreds of cells, allowing for the observation of electrical pulses traveling between neurons.
Researchers from University of Houston release open-source dataset and instructions for building a smartphone microscope with an inexpensive inkjet-printed elastomer lens. The device can perform fluorescence microscopy, detect waterborne pathogens, and has potential applications in rural areas and developing countries.
Researchers developed a microfluidic chip-based platform for analyzing live cells using fluorescence microscopy. The platform uses a CMOS image sensor and allows for fully automated systems, making it suitable for high-throughput applications.
Researchers at Nagoya University have developed a new photostable fluorescent dye, PhoxBright 430 (PB430), to visualize cellular ultrastructure. PB430 enables continuous STED imaging and can be used for multicolor imaging of biological structures.
Researchers used a new, non-destructive technology to access medieval texts hidden inside ancient bookbindings. By fusing visible hyperspectral and X-ray fluorescence imaging, they discovered sixth-century Roman Law code written in marginal comments.
A new nanoparticle 'buckyswitch' developed by Clemson researchers improves microscopic imaging resolution by allowing microscopes to capture images up to the terapixel level. This innovation overcomes the diffraction limit constraint and enables clearer visualization of small cellular structures.
Chulhong Kim, a POSTECH Associate Professor, has received the IEEE-EMBS Early Career Achievement Award for his contributions to biomedical imaging. His lab developed world-first photoacoustic gastro-intestinal tract imaging using organic agents.
Scientists at Goethe University Frankfurt have combined two advanced fluorescence microscopy techniques to observe cells with high-resolution imaging. The new technique, called csiLSFM, allows for three-dimensional insight into a cell's interior with sub-100nm resolution.
Researchers developed a dual-modality imaging technique using SPECT/CT and fluorescence to detect micrometastases in colorectal cancer. This approach can guide resection of tumor lesions and potentially improve prognosis for cancer patients.
Researchers have developed a new technique to track the movement of cancer cells using reversible, photo-luminescent carbon nanoparticles. The study demonstrates that these particles can be used for intracellular imaging and drug delivery tracking without photo-bleaching issues.
Researchers have developed an image analysis technique called Hyper-Spectral Phasor (HySP) that can track multiple molecules in living organisms, making it easier to diagnose diseases and identify therapeutic targets. The new technology uses cell phone images and is faster and less expensive than current methods.
Scientists have discovered metabolons, complex enzyme clusters, for the first time using molecular movie technology. This breakthrough reveals plants' secret medicinal toolbox and unlocks new possibilities for harnessing plant-based medicines.
Scientists have created peptide probes that attach to proteins with comparable efficiency to antibodies, improving image resolution. These probes can help shed light on protein layout and quantification, opening new possibilities for neurobiological research.
Scientists at MIT and Harvard University developed a new imaging technique called LASE microscopy, which uses tiny particles to create sharper images of deep tissue and cells. The particles emit laser light when stimulated by a laser beam, resulting in higher-resolution images.
Researchers at University of California San Diego developed a new form of multicolor electron microscopy allowing simultaneous visualization of multiple molecular species. This technique reveals details and processes not visible in grayscale images, providing new insights into cellular ultrastructure.
A study by Lund University researcher Pontus Nordenfelt reveals how cells move using integrins, actin, and an adaptor protein. The technique enables measuring mechanical force acting on integrins, which could lead to targeted drugs to strengthen the immune system against infections.
CAS researchers have developed a new monomer fluorescent protein, Skylan-NS, enabling substantial improvements in the speed, duration, and noninvasiveness of live-cell superresolution microscopy. The protein shows high photostability, cycle numbers and signal-to-noise ratio, making it suitable for live-cell SR imaging.
Scientists have developed a multi-view microscope that captures higher-resolution images of live cells and tissues without increasing radiation exposure. The new system uses computation to fuse images and achieve double the volumetric resolution of traditional methods.
Researchers developed a new method to capture three views simultaneously, producing more detailed perspectives of bacteria and living cells. The technique improved volumetric resolution by up to 235nm, doubling the resolution of traditional methods.
A new platform called spectroscopic photon localization microscopy (SPLM) increases the resolution of molecular imaging by fourfold, making it faster and simpler. This breakthrough can be applied to various fields like materials science and life sciences to study nanoscale environments.
Researchers have developed a method to observe nanometer-sized patterns of biomolecules such as proteins in an arrested but living state. This allows for the recording of molecular activity and interactions without causing cell death, revealing new insights into cellular behavior and processes.