A proof-of-concept study promises the speedy diagnosis of lower respiratory tract infections through analysis of human breath. Lower respiratory tract infections, such as bronchitis, pneumonia, and bronchiolitis, are currently diagnosed by culturing bacteria from respiratory specimens, but the procedure is invasive, laborious, and time intensive. Molecular sequencing techniques cannot differentiate between clinical infection and mere colonization, in which bacteria are benignly present in the respiratory tract. Dapeng Chen and colleagues have designed a medical device that measures the protease activity of human neutrophil elastase in human breath with high sensitivity. The over-release of neutrophil proteases is an effect caused by lower respiratory tract infections. The authors tested the system on clinical breath samples from intubated patients as well as a group of healthy volunteers. The platform measured elevated levels of human neutrophil elastase in patients diagnosed with confirmed lower respiratory tract infections. According to the authors, the results suggest that developing a breath-based diagnostic tool for lower respiratory tract infections will be possible, which could be particularly useful in intubated patients within critical care settings.
PNAS Nexus
A breath-based in vitro diagnostic assay for the detection of lower respiratory tract infections
24-Sep-2024
D.C., K.M.K, C.R.H., E.R.C., M.S.C., W.A.B., and M.M. have competing interests. D.C., K.M.K., C.R.H., M.S.C., and E.R.C. are employed by Zeteo Tech, Inc. W.A.B. and M.M. serve as chief executive officer and chief technology officer for Zeteo Tech, Inc., respectively. M.A.M., Y.P., and S.C. have no competing interests. An unpublished US Provisional Patent Application assigned to Zeteo Tech, Inc. was applied based on this research.