I n a preclinical study out of Mass General Brigham, researchers found that even short-term exposure to high levels of dietary fat results in a quick and selective loss of critical gut immune cells called ILC3s, promoting intestinal permeability and inflammation.
Selma Boulenouar, PhD , of Mass General Brigham Neuroscience Institute , is the senior author of a paper published in Immunity , “ High-Fat Diet Causes Rapid Loss of Intestinal Group 3 Innate Lymphoid Cells Through Microbiota-Driven Inflammation and Cellular Metabolic Stress .”
Q: What challenges or unmet needs make this study important?
Chronic diseases such as obesity, inflammatory bowel disease, colorectal cancer and neuroinflammatory conditions are rising globally and are closely linked to diet and gut inflammation. However, the earliest immune events triggered by dietary fat remain poorly understood. In particular, our lack of understanding around how diet rapidly reshapes immune activity in the intestine—home to roughly 70% of the body’s immune cells—has been a major gap in the field. Most studies focus on long-term effects, but patients are exposed to dietary changes daily. Understanding the immediate impact of a Western-style diet on gut immunity is essential to identify early drivers of disease and potential intervention points.
Q: What central question(s) were you investigating?
We asked whether short-term exposure to a high-fat diet could directly alter key immune populations in the gut, and if so, through what mechanism. Specifically, we focused on group 3 innate lymphoid cells (ILC3s), which are established early in life and play a critical role in maintaining intestinal barrier integrity and preventing inflammation. We also investigated why these cells might respond differently compared to closely related immune cells, such as Th17 cells.
Q: What methods or approach did you use?
We combined mouse models of diet-induced obesity with analyses of human intestinal samples to assess immune cell changes following high-fat diet exposure. We used flow cytometry, transcriptomics and metabolic assays to characterize immune cell function and survival. In addition, we applied live-cell imaging approaches to track lipid metabolism and mitochondrial health in immune cells. The use of germ-free and genetically modified mouse models allowed us to determine the contribution of the microbiota and inflammatory signaling pathways.
Q: What did you find?
We found that even short-term exposure to a high-fat diet rapidly and selectively depletes ILC3s in the intestine. This effect is driven by microbiota-induced inflammatory signals that disrupt the cells’ ability to process lipids, leading to mitochondrial dysfunction and cell death. In contrast, Th17 cells remain largely unaffected.
Importantly, the loss of ILC3s reduces IL-22 production (which normally helps protect the gut lining), promotes intestinal permeability or “leakiness” and drives inflammation. These findings reveal a rapid collapse of an immune system established early in life in response to dietary fat.
Q: What are the real-world implications, particularly for patients?
These findings identify an early and previously unrecognized mechanism by which diet can rapidly impair gut immunity. The loss of ILC3s compromises intestinal barrier function and may contribute to a wide range of chronic diseases linked to inflammation. Importantly, this process appears to be reversible with dietary changes, suggesting that early interventions could help restore immune balance. More broadly, this work highlights immune metabolism as a potential therapeutic target.
Q: Were you surprised by any of the findings?
We were particularly surprised by the speed and specificity of the response. ILC3s are seeded early in life and are typically considered resilient cells. Observing their rapid depletion within days of dietary exposure was unexpected. Equally striking was the selective vulnerability of ILC3s compared to Th17 cells, revealing a previously unappreciated difference in how immune populations handle metabolic stress.
Authorship: In addition to Selma Boulenouar, Mass General Brigham authors include Eva C. Torrico, Paulien Kaptein and Fatiha Laalouhmi as the lead authors with equal contribution. The full list of authors can be found in the paper.
Paper cited: Torrico, E.C., et al. “High-Fat Diet Causes Rapid Loss of Intestinal Group 3 Innate Lymphoid Cells Through Microbiota-Driven Inflammation and Cellular Metabolic Stress.” Immunity. DOI: 10.1016/j.immuni.2026.02.014
Funding: This study was funded by the Ann Romney Center for Neurologic Diseases at Brigham and Women’s Hospital and Bruxelles Wallonie International (BWI) in Belgium. Additional institutional and collaborative funding sources supporting this work can be found in the paper.
Disclosures: None.
URL for CTA: https://www.cell.com/immunity/fulltext/S1074-7613(26)00084-1
Immunity
Randomized controlled/clinical trial
Animals
High-fat diet causes rapid loss of intestinal group 3 innate lymphoid cells through microbiota-driven inflammation and mitochondrial stress
30-Mar-2026