Articles tagged with Dna Sequencing
Researchers at Columbia University have developed a novel approach for single molecule electronic DNA sequencing that can accurately distinguish four DNA bases using nanopores. The technique, called Nano-SBS, uses distinct chemical tags to label DNA building blocks, overcoming the challenge of small differences among the four nucleotides.
Researchers used DNA analysis to separate hundreds of coral symbionts into distinct species, revealing unique ecological distributions. This breakthrough should improve research into reef-building corals and their complex biology, potentially aiding in the survival of corals under global warming.
Researchers encoded George Church's book 'Regenesis' in DNA using novel strategy and next-generation sequencing technology. The team stored 54,898 unique DNA sequences, each with an address to guide reassembly, for a total of 70 billion copies.
Researchers have discovered an RNA-based complex that guides a DNA-cutting enzyme to specific sites, enabling easy customization for laboratory applications. This breakthrough could revolutionize genome editing and gene function studies, offering a powerful tool for biotechnology efforts.
The new study proposes a way to effectively introduce carefully planned DNA segments into genomes of living cells and test the effects. The technology enables simultaneous introduction of tens of thousands of DNA regions into tens of thousands of living cells, allowing for precise measurement of results within a single experiment.
A new software tool called Acacia has been developed to correct errors in DNA sequencing, allowing biologists to accurately interpret genetic information. The tool, created by CSIRO PhD student Lauren Bragg, uses statistical theory to analyze code for DNA bases and improve accuracy.
A global team of scientists led by Virginia Tech and the University of Tuscia has successfully traced the origin of the devastating kiwifruit pathogen Psa back to its likely source in China. The research used DNA sequencing technology to compare bacteria samples from around the world, revealing that they were nearly identical except fo...
A study using ancient DNA from archaeological sites found that gray whales had a substantially larger population before whaling and experienced a sharp recent decrease, consistent with whaling as the cause. The research suggests a pre-whaling decrease in population size of about 78,000 to 116,000 individuals.
Researchers from Delft University of Technology have discovered a crucial step in the DNA repair process, revealing how a broken DNA molecule efficiently searches for a matching sequence. The discovery uses a dual-molecule technique to clarify why certain sequences lead to quick dissociation while others form strong bonds.
Researchers have created a method to sequence epigenetic marks 5hmC and 5mC in DNA at single base resolution, improving our understanding of gene regulation and cell development. This breakthrough has major implications for regenerative medicine and stem cell research.
Researchers at TUM developed DNA origami 'gatekeepers' that can filter biomolecules by size, allowing selective detection of specific target molecules. The device combines solid-state nanopores with custom-designed DNA structures for enhanced single-molecule sensing capabilities.
Researchers have developed a nanoscale sensor that can electronically read the sequence of a single DNA molecule, leading to potential breakthroughs in personalized medicine. The technique is fast and inexpensive, making it possible to reveal predispositions for afflictions like cancer, diabetes, or addiction.
Researchers have successfully diagnosed a new species of plant using DNA barcoding, a method that relies on short DNA sequences for identification. The study marks a significant shift towards English-language diagnoses, which will enable scientists to more easily share and verify findings.
Scientists at the University of Nottingham used next-generation DNA sequencing to correct a patient's genetic diagnosis from incorrect to accurate. The new technique enables fast and affordable analysis of human genomes, providing hope for thousands of people living with muscle-wasting diseases in the UK.
Researchers sequenced the Tasmanian devil cancer genome to understand its origin and spread. The study found that the cancer arose from a single female Tasmanian devil and has genetically diverged during its spread, indicating some sub-types may be more virulent.
Researchers have developed a new catalogue of loss-of-function (LoF) variants to better understand the normal function of human genes. The study identifies over 1000 LoF variants, some of which are rare and potentially harmful, while others may not have a significant effect on health.
Researchers have cataloged all transposable elements in a population of fruit flies using Pool-Seq technology. The findings reveal that most elements are purged before becoming established, but some sites show positive selection for insertion, suggesting beneficial effects on the host.
Researchers at UCSF are sequencing the DNA of 4,000 people with various forms of epilepsy in a bid to identify the genetic causes of the disease. The study, funded by a $25-million grant, will also explore ways to treat people with epilepsy and uncover new patterns of genetics.
Researchers have developed a software package called PoPoolation2 to compare population data, facilitating the study of evolution and adaptation. The tool uses next-generation sequencing methods to determine allele frequencies between populations, providing insights into evolutionary processes.
Scientists map typhoid outbreaks in Kathmandu using gene sequencing and GPS technology, revealing clusters near water spouts and lower elevations are at high risk. The study also sheds light on asymptomatic carriers' role in disease spread.
Researchers led by Robert Linhardt of Rensselaer Polytechnic Institute have sequenced the first complex carbohydrate biopolymer, bikunin. The discovery provides a fundamental new view of these vital biomolecules, which play roles in cell structure and development, disease pathology, and blood clotting.
Berkeley lab researchers have discovered a complex protein structure in E.coli that plays a critical role in defending against viruses and other invaders. The 'Cascade' complex acts as a surveillance system, detecting and inactivating invading pathogens using RNA-guided target binding.
Scientists identify genetic differences between mild and deadly Plague bacteria, revealing the role of small non-coding RNAs in disease severity. The study provides new insights into the evolution of pathogens and potential therapeutic targets for deadly diseases like the Plague.
Researchers at the University of Southampton are developing a novel device to directly sequence single DNA strands, aiming to improve genome analysis. The new method could provide faster and cheaper DNA sequencing, eliminating complex biochemical processes.
Researchers have created a detailed map of gene expression in the mouse cerebral cortex, which shares 90% of its genes with humans. The atlas provides insight into how genes work in this complex region of the brain, including correlations between specific genes and human diseases such as Parkinson's and Alzheimer's.
An international team of scientists used single molecule, real-time DNA sequencing technology to analyze the pathogenicity and evolutionary origins of the highly virulent German E. coli outbreak strain. The results provide the most detailed genetic profile to date, highlighting the importance of DNA sequencing in understanding how bact...
Researchers used whole genome sequencing and social network analysis to track a tuberculosis outbreak in British Columbia, identifying key individuals as superspreaders and behaviors contributing to the outbreak. The technique allowed public health officials to reconstruct outbreaks and understand how pathogens move through populations.
RIKEN researchers developed a new gene expression analysis technique called HeliScopeCAGE that can measure gene expression levels using only 100 nanograms of total RNA. This technique reduces biases and generates highly reproducible data, enabling high-precision gene expression analysis from tiny samples.
ScienceBuddies.org engages students in hands-on science, attracting 9.8 million unique visitors in 2010. The site offers personalized learning tools and features over 1,000 project ideas to ignite passion for learning.
Cheryl A. Kerfeld, a structural biologist, has won the American Society for Biochemistry and Molecular Biology's Award for Exemplary Contributions to Education for her innovative approach to teaching genomics and bioinformatics. Her educational programs have been adopted by over 65 institutions, empowering undergraduate students to ann...
Researchers found that two genetic mutations can trigger hypertension in severe cases, affecting up to one-third of patients with a common cause. The mutations affect potassium channels and aldosterone release, leading to adrenal tumor formation and severe high blood pressure.
A new noninvasive test for trisomy 21 has been developed using DNA sequencing of maternal blood plasma, accurately detecting the extra chromosome in 100% sensitivity and 99.7% specificity. The test shows promise as a potential alternative to invasive prenatal testing.
A research project aims to analyze the genomes of 250 prostate cancer patients under 50 to identify genetic mutations that cause and promote the disease. The goal is to produce a comprehensive map of genetic modifications involved in prostate cancer, which may lead to new treatment approaches and diagnostic methods.
Researchers from Imperial College London have developed technology that could sequence a human genome in mere minutes, potentially unlocking personal susceptibility to diseases. The technology uses nanopores and could lead to fast, inexpensive genome sequencing with numerous benefits for medical tests and DNA profiles.
The study provides the first whole genome sequence analysis of the Haitian Vibrio cholerae outbreak strain, confirming a South Asian lineage. This understanding has important public health policy implications for preventing future cholera outbreaks.
Biophysicist Stuart Lindsay's new technique uses recognition molecules to grasp each base in turn, generating a distinct electronic signal that identifies each base. This allows for the reading of individual bases without interference from neighboring bases, including recognition of epigenetic modifications.
The latest phase of the HapMap Project reveals rare genetic variants distributed unevenly among populations, with some genes under selection in different populations. The study provides a framework for future genetic studies of variation and disease, highlighting the importance of examining diverse populations.
Researchers discover genetic alterations in the MLL2 gene that account for most cases of Kabuki syndrome, a rare disorder with multiple birth defects and mental retardation. The new DNA sequencing strategy quickly identifies the gene variants responsible for the condition.
Researchers at Sandia National Laboratories have developed kinked nanopores that can slow down DNA transmission, enabling easier DNA sequencing. The innovation uses self-assembly techniques and atomic-layer deposition to achieve a fivefold slowdown in voltage-driven translocation speeds.
Researchers analyze genomes of two individuals with mixed ancestry to study the genetic basis of complex diseases. The study provides a clearer picture of the ancestry of genome sequences for individuals studied, revealing the approximate number of generations at which genetic mixing occurred and estimating the rate of admixture.
Scientists at Oak Ridge National Laboratory have identified a key gene for improving the conversion process of cellulosic biomass into ethanol. The modified microorganism shows enhanced efficiency and potential use for more cost-effective biofuel production, overcoming current pretreatment challenges.
A new nanopore method for DNA sequencing has been developed by Boston University researchers, enabling ultra-fast and low-cost genetic analysis. The technique uses solid-state nanopores to detect DNA molecules, achieving readout rates of up to 200 bases per second.
The European Nucleotide Archive (ENA) consolidates three major sequence resources, providing free access to over 20 terabases of nucleotide sequence data. The ENA offers improved submission and data-access tools, making it easier for users to share their sequence data.
A new approach detects rare but powerful causal gene variants, accounting for significant proportions of the 'missing heritability' problem in common diseases. The technique may identify individual patients with specific mutations, enabling more meaningful diagnostic results and potential treatments.
Scientists have discovered DNA from the agave butterfly caterpillar in mescal liquor, confirming their theory that preserved specimens can leak into preservative liquids. This breakthrough allows for non-invasive genetic analysis protocols, reducing the need for tissue samples or DNA extraction.
ASU professors Stuart Lindsay and Paul Westerhoff will lead two innovative projects to tackle challenges in rapid DNA sequencing and nanotechnology health risks. The projects aim to simplify DNA sequencing like the invention of the transistor simplified electronics.
A DNA study confirms that Africa's rarest monkey, the kipunji, had an intriguing sexual past with baboons. The results may help to set conservation priorities for this critically endangered species by highlighting the importance of protecting its habitat and preserving genetic diversity.
A Yale team used whole exome sequencing to identify a mutation in a Turkish baby's genome, leading to a surprise diagnosis and treatment of congenital chloride diarrhea. The approach has potential for dramatic insights into human diseases and could become commonly used in clinical settings.
Researchers at Cold Spring Harbor Laboratory have developed a sensitive and accurate way of identifying gene copy number variations (CNVs) using new DNA sequencing technologies. This method allows for the detection of small structural variants that were previously overlooked by current methods.
Researchers at Cold Spring Harbor Laboratory have devised 'DNA Sudoku,' a strategy that allows for the simultaneous sequencing of tens of thousands of DNA samples. This approach combines concepts from cryptology and combinatorial number placement rules, reducing costs dramatically compared to conventional methods.
A new technique using scanning tunneling and atomic force microscopes can identify complementary DNA base pairs by measuring the strength of hydrogen bonds. This method could lead to a new DNA sequencing technology that is faster and cheaper than current methods.
Scientists from the University of Cambridge discovered four rare genetic mutations that reduce the risk of developing type 1 diabetes, a link between T1D and enterovirus infections established. The IFIH1 gene variants were found to protect against T1D by reducing immune activation caused by enterovirus infections.
A study of ancient DNA from 68 skeletal remains reveals a rapid evolution in the Icelandic mtDNA pool over the past thousand years. The original female settlers appear to be genetically less closely related to present-day Icelanders than previously thought.
Researchers at UC San Diego and Genentech have developed a new shotgun protein sequencing method that reduces the time required to sequence an unknown antibody to under 36 hours. This breakthrough technique is faster than traditional methods and can automatically detect post-translational modifications.
DNA's photostability is influenced by its base sequences, with different combinations exhibiting varying fluorescence lifetimes. This discovery could lead to new diagnostic methods and potentially repair gene mutations using laser radiation.
Researchers at Howard Hughes Medical Institute have created a non-invasive blood test that accurately detects Down syndrome and two other serious chromosomal defects. The test is more accurate than previous methods and can provide results within a few days, reducing the wait time for anxious parents.
Scientists have found a novel linear chromosome in cyanobacterium Cyanothece 51142, containing genes important for producing biofuels. The discovery was made possible by simultaneous DNA sequencing and protein analysis, which revealed more genes on the linear and circular chromosomes than previously thought.
A study published in the American Journal of Human Genetics reveals a significant presence of mitochondrial DNA mutations in the general population. Researchers found that at least 1 in 200 individuals harbor pathogenic mitochondrial DNA mutations, which may lead to diseases such as muscle weakness and diabetes.
Researchers have developed a new computational method to reconstruct viral populations with improved accuracy and efficiency. This method, based on pyrosequencing, reduces error rates and yields faster results at a lower cost.
Researchers have identified a gene sequence associated with an expanding waist line, weight gain, and insulin resistance, affecting half of the UK population. The sequence is more common in Indian Asians than Europeans, providing a possible genetic explanation for the high levels of obesity and diabetes in this group.