Articles tagged with Cell Lines
Recent studies have raised safety concerns over CRISPR's precision in human cell lines, but companies are forging ahead with therapies. The technology makes double-stranded breaks at specific sites in DNA to repair defective genes.
Researchers have identified valuable pools of genetic material from cryopreserved cell lines, which may help guide a tailored recovery program for the northern white rhinoceros. The study's findings suggest that advanced sequencing technologies and novel reproductive strategies could improve recovery efforts for other endangered species.
Scientists create cell models with specific DNA repair gene defects, allowing them to identify genomic scars associated with these defects. These scars can serve as biomarkers for molecular characterization of tumors and improve personalized cancer treatment.
Research findings show that PD-L1 expression facilitates immune escape in medulloblastoma patients. The study identifies subgroup-specific variations in PD-L1 expression and its relationship with therapeutic responses. Immune adjuvant therapies may be necessary to fully realize the benefits of PD-1 blockade treatments.
Researchers at the University of Bristol have developed a method to enhance red blood cell transfusion compatibility using CRISPR-Cas9 gene editing. By altering specific blood group genes, they can prevent immune reactions in patients with rare blood types or those requiring frequent transfusions.
Scientists at Oregon Health & Science University have developed a new method to quickly and efficiently recognize the subtypes of cells within the body. This technology, led by Andrew Adey, allows for profiling thousands of cells simultaneously, improving our understanding of disease at the molecular level.
Researchers at Duke University created a framework to determine when using multiple cell populations is beneficial. The system models how variables interact in complex bioengineering tasks, revealing that efficiency and growth rate are key factors. This tool can help industries producing chemicals with bacteria, such as pharmaceuticals...
Researchers created synthetic neurons from a readily available cell line to investigate purine synthesis in the human brain. The study found that the core enzyme FGAMS is expressed throughout neurons and non-neuronal cells, with infection by herpes simplex virus affecting its expression and clustering.
Researchers found that ibuprofen modified hormonal profiles through selective transcriptional repression of testicular endocrine cells, leading to a state of compensated hypogonadism. The study used testis explants and human steroidogenic cell lines to investigate the effects of ibuprofen on testicular health.
Researchers found that human APOBEC3H strongly inhibited SIVcpz due to its resistance to Vif proteins. The study suggests that human APOBEC3H may protect against HIV-1 transmission from chimps, particularly in individuals with unstable forms of the protein.
A study from the University of Pennsylvania reveals how defective viral genomes can help infected cells survive despite an immune system attack. The researchers discovered that signaling proteins encoded by these genomes protect infected cells from apoptosis, enabling them to persist in the face of an antiviral response.
The University of Liverpool's Tick Cell Biobank has received £940,000 funding to expand its tick cell line collection globally. The Biobank will establish outposts in South-East Asia, Africa and South America to support research into tick-borne diseases.
Researchers have successfully edited the pig genome to deactivate a family of retroviruses, which can be passed on to human cells. The achievement holds important implications for transplant medicine and may one day enable pig-to-human organ transplants.
Researchers have identified a new molecular target, OR51B4 olfactory receptor, in colorectal cancer cells, which can be activated by the odorant troenan. This activation leads to inhibition of tumour growth, slowing down cell proliferation and increasing apoptosis.
The OHSU Knight Cancer Institute research team discovered a previously unreported flaw in the standard leukemia assay, which can lead to non-functional mutations appearing functional. They recommend an additional sequencing step to improve the assay's accuracy and reproducibility.
Researchers from DTU Biosustain have engineered E. coli cells to produce large amounts of serine, a compound used in detergents and pharmaceuticals. The cells can tolerate high concentrations of serine and produce up to 250-300 grams per kg of sugar added.
Researchers successfully developed a vaccine against influenza using genetically modified live virus that activates the immune system but cannot replicate in healthy cells. The new vaccine proved effective in mice, guinea pigs, and ferrets, offering an antibody response comparable to existing vaccines.
Researchers at the University of Southampton have engineered human cells with a genetic circuit that produces a molecule inhibiting HIF-1, allowing cancer cells to survive in nutrient-limited environments. The study demonstrates the potential for using sentinel circuits to target diseases like cancer.
Researchers from Colorado State University discovered that a cancer cell's shape may offer similar clues to predict how dangerous it is and guide treatment strategies. By analyzing cell shape using Zernike moments, the team was able to identify patterns that distinguish invasive osteosarcoma cell lines.
A team of researchers has successfully isolated Zika virus from a semen sample, obtaining the first complete genome sequence of the virus in humans. The study provides valuable insights into potential sexual transmission of Zika virus, which was previously poorly understood.
Researchers found that increased cell membrane fluidity enhances auranofin's ability to induce DNA damage and cellular oxidation in ovarian cancer cells. This could lead to improved treatment outcomes by increasing the drug's effectiveness against cancer cells with fluid membranes.
A commonly used cell line, U87MG, was found to have a different DNA profile than the original tumour it was established from. Genetic analysis revealed that the cell line likely originated from a human glioma tumour, highlighting the need for proper identification of cultured cells.
A new approach using patient-derived cell lines has revealed that pancreatic tumors have diverse genetic mutations, making it challenging to target treatments. The study suggests that personalized medicine can be improved by testing drugs against each patient's specific tumor genetics.
Researchers found that inhibiting RMEL3, a non-coding RNA, reduces melanoma cell proliferation by up to 95%. The study suggests RMEL3 is expressed in most cases of melanoma and offers promising therapeutic and diagnostic potential.
Researchers found that the expression of specific genes and DNA methylation patterns, such as IFG2 and reduced aberrant methylation, are better indicators of iPS cell differentiation to hematopoietic cells. This discovery suggests a more efficient method for producing high-quality blood cells through reprogramming.
A worldwide competition of engineered cells through microfluidic mazes revealed that neutrophil-like cells must balance speed against chemotactic accuracy. An adapted version of the amoeba Dicty with increased activity of a particular protein, Ric8, emerged as the most successful strategy.
The National Institute of Standards and Technology calls for community action to develop comprehensive toolkit for assuring cell line quality. A DNA fingerprinting method is proposed to detect mix-ups and contamination, addressing a growing priority among funders and publishers.
Scientists from the National Institute of Standards and Technology (NIST) urge researchers to adopt comprehensive toolkit for assuring cell line quality, implemented before studies using human cells. Current standard has not been widely adopted despite growing priority among funders and publishers.
Researchers identified a lymphatic endothelium origin for angiomyolipoma and lymphangioleiomyomatosis, two related tumors with previously unknown cellular origins. The study suggests a potential novel origin for LAM mechanistically dependent upon TSC inactivation as well.
Researchers have found the lock for a cellular 'trap door' that could lead to more efficient and less harmful delivery of proteins into cells. This breakthrough has potential applications in treating diseases such as HIV, cancer, and regenerative medicine.
Researchers developed a cell-based model of the human placenta that can help explain how pathogens, like Zika virus, cross from mother to unborn child. The model recapitulates the barrier properties of naturally occurring cells and resists infection by certain viruses.
Scientists have isolated a new type of herpes virus from bat cells, providing insights into the biology of these viruses and their potential to transmit to humans. The discovery could aid in understanding why bats are often associated with viral infections that can be transmitted to humans.
Researchers developed logic gates that can operate inside cells and interact with native messenger RNA, enabling a foundation for bio-computers to sense, analyze, and modulate molecular information. The tools could provide a basis for creating circuits with many inputs to control cellular behavior in response to stimuli.
Researchers at the University of Copenhagen have created a novel cell line to screen brain drugs, mimicking the blood-brain barrier's impermeability. This tool may accelerate the development of improved treatments for brain diseases like Alzheimer's and Parkinson's.
Researchers found that e-cigarette vapor damaged human cells, causing DNA breaks and cell death. The study suggests that e-cigarettes are not as safe as their marketing claims, and may contain previously undiscovered carcinogenic components.
A team of researchers at UCSF developed ORACLs, a lineage of genetically modified cancer cells that change their appearance when treated with drugs, inspired by face-recognition software. These cells can quickly organize molecules in institutional compound libraries according to their biological function.
Researchers have successfully used the CRISPR-Cas9 system to eliminate porcine endogenous retroviruses (PERVs) from pig epithelial cells. The study found that PERV infectivity was reduced by up to a thousand fold, making genetically modified pigs for human transplantation a promising option.
Researchers have identified a two-molecule sensing mechanism that allows human cells to detect electric fields, opening up new avenues for understanding wound healing and cellular movement.
Researchers from UNIGE and Trento University have deciphered the mechanism by which HIV infectivity is destroyed, revealing a new antiretroviral protein called SERINC5. SERINC5 enhances cell defense against HIV, rendering Nef's ability to neutralize it ineffective.
Researchers at Rockefeller University have discovered a single gene, SEC14L2, that allows the easy replication of hepatitis C virus in human liver cancer cell lines. This breakthrough enables scientists to study naturally occurring forms of the virus and develop new effective treatments.
A crowdsourcing challenge in systems biomedicine has combined predictions from hundreds of scientists to estimate population-level response to toxic compounds. The study provides new methodologies for hazard evaluation and assessment, offering a way to accelerate toxicity testing.
Researchers developed a biosensor to measure Mitochondrial Pyruvate Carrier (MPC) activity in malignant cells, finding low MPC activity compared to healthy cells. Treating cancer cells with a new compound restored normal MPC activity, suggesting the carrier's dysfunction is responsible for its inactivity.
Researchers at Hiroshima University found that long noncoding RNA CCDC26 controls receptor tyrosine kinase KIT expression in leukemia cells. This discovery provides new insights into leukemia recurrence and may help develop new therapies.
A study by Genes and Cancer group at Bellvitge Biomedical Research Institute found that the inactivation of PARD3 gene promotes tumor cell invasion and metastasis in lung cancer. Restoring PARD3 levels significantly reduced the risk of metastasis in animal models.
A new platform called GUIDE-seq detects unwanted DNA breaks induced by CRISPR-Cas RGNs across the entire human genome. The method is sensitive enough to detect off-target sites at a frequency as low as 0.1 percent in a population of cells.
A recent study found that cell division in endothelial cells leads to the formation of large, ordered eddies in tissue, which may help widen blocked blood vessels and aid healing. The researchers used phase-contrast microscopy to observe the movement of new cells and found characteristic turbulence patterns.
Researchers at Massachusetts General Hospital describe a new screening platform that combines genetic and pharmacologic screening of tumors, enabling truly individualized treatment regimens. The approach identifies previously unknown resistance mechanisms, several of which were not detectable by gene sequencing alone.
Researchers have found that microRNA isoforms are more common than initially assumed, with certain variants exhibiting population-dependent and gender-dependent expression profiles. These findings suggest that miRNAs may serve a purpose beyond regulation of protein-coding genes.
Researchers identify RET M918T mutation in SCLC tumors, which drives increased ERK signaling and MYC expression. The mutation makes cells sensitive to tyrosine kinase inhibitors ponatinib and vandetanib.
Researchers at Massachusetts General Hospital developed a microchip-based device to capture and culture circulating tumor cells (CTCs) for genetic analysis and drug testing. The study found that cultured CTCs accurately reflect a tumor's genetic mutation over time, revealing new mutations and potential drug targets.
Using the subway analogy, a physicist is applying queuing theory to study protein traffic jams in cells. By understanding these bottlenecks, he aims to discover mechanisms for alleviating them and develop new tools for synthetic biology. This research has the potential to impact areas such as development, inflammation and cancer.
Researchers have identified twenty specific proteins secreted by primary effusion lymphoma cell lines, which may explain the cancer's peculiar behavior. These proteins are involved in inflammation, immune response, and cell growth, and their presence in tumor cells could lead to more personalized treatment approaches.
Researchers at Dartmouth have developed a protocol to harvest and culture mouse melanoma cells, which are rare in human form but prevalent in mice. This breakthrough allows for more accurate modeling of human melanoma, potentially leading to new treatments.
Researchers track cellular events leading to cardiac regeneration in zebrafish, revealing a novel potential source of cells for repairing damaged heart muscle. The study's findings provide evidence that various cell lines in the heart are more plastic than previously thought, offering new hope for treating heart failure.
Mount Sinai researchers discover that diabetics using glucose meters at appointments have improved disease control. A study found that patients with controlled diabetes had a lower Hemoglobin A1c level compared to those without monitored glucose levels.
Researchers at the University of Southern Denmark have developed a new technique to test drugs on human liver cells, reducing the risk of fatal effects. By growing cells in 3D structures that mimic natural human body conditions, scientists can better predict how substances will behave in humans.
Researchers identified biochemical changes in cancer cells that render them vulnerable to PARP inhibitors, which showed promise as a novel treatment strategy for cisplatin-resistant cancer. The study found that high levels of poly (ADP-ribose) polymerase 1 and elevated amounts of poly (ADP-ribosyl) were linked to resistance to cisplatin.
Researchers at Harvard Medical School have identified a signaling pathway that may determine genetic susceptibility for the development of FASD. The study found that phosphorylation events inside cells can render L1 adhesion molecules more vulnerable to alcohol's inhibitory effects, providing new targets for diagnosis and therapy.
Researchers at Johns Hopkins Kimmel Cancer Center create cell lines from patients' own tumors to predict chemotherapy sensitivity. The new method may replace current laboratory tests and improve drug selection accuracy.
Researchers at UNIGE developed a screening technique called Synthetic Tandem Repeat PROMoter (STAR-PROM) to identify transcription factor serum response factor (SRF), which activates genes and influences cellular structure. The technique allows for the discovery of factors modulating gene expression in various contexts, including drug ...