Articles tagged with Immunofluorescence Analysis
The platform enables simultaneous visualization of multiple hepatic cell populations, including hepatocytes, cholangiocytes, macrophages, endothelial cells and hepatic stellate cells. It also extends multiplex imaging beyond tissue sections to organoids, primary liver cell cultures and advanced liver-on-a-chip platforms.
Researchers at North Carolina State University found that soft tissue preservation in fossils does not seem to depend on the species, age or burial environment. The team was able to retrieve vessels from six dinosaur specimens, including four Tyrannosaurus rex and one Brachylophosaurus canadensis, using a suite of analytical tools.
A study published in Chinese Medical Journal explores the use of artificial intelligence to identify potential medications for treating glaucoma. Researchers used AI models to predict the effectiveness of small-molecule compounds targeting RIPK3, a key signaling molecule involved in programmed cell death.
A new rapid test has been developed to identify hantavirus in South Korea, paving the way for early outbreak control. The Flongle sequencing-based diagnostic is cost-efficient and can detect HTNV genomes within 3 hours.
A new study reveals that RNA binding motif protein X-linked (RBMX) plays a crucial role in predicting cancer prognosis and response to immunotherapy. Abnormal expression of RBMX is associated with immune regulation, tumor microenvironment, and therapeutic effects of immune checkpoint inhibitors.
Researchers have identified a novel therapeutic target for pelvic pain in endometriosis by focusing on the IL-1β pathway and its regulation via the JNK signaling pathway. This study suggests that JNK inhibitors may be effective in reducing neuroinflammation and alleviating symptoms of endometriosis-associated pain.
A new multiplex assay has been developed to assess activated p300/CBP in circulating prostate tumor cells, revealing correlations with clinical outcomes and potential therapeutic targets for castration-resistant prostate cancer. The study found that patients with upregulated p300/CBP activity had shorter response times to ARSI therapy.
Scientists have found that antibodies targeting βII-spectrin on mesangial cells are the trigger for IgA nephropathy. This discovery enables blood-based diagnosis and may lead to improved treatments.
Researchers at Okayama University discovered genes and proteins responsible for the rapid contraction of axopodia in Heliozoa, a group of eukaryotes. The study identified key players in microtubule disruption, including katanin p60, kinesin, and calcium signaling proteins.
A team of researchers identified the precise mechanism of nuclear envelope repair, finding that lamin C, BAF, and cGAS work together to facilitate rapid repair. The study provides insights into rare genetic disorders such as laminopathies and has potential applications for understanding and treating related diseases.
Researchers develop conditionally active immunofluorescence probe, C11_Fab Q-body, for imaging p53 biomarker protein in live cancer cells. The probe displays high sensitivity and target specificity, enabling precise visualization of intracellular dynamics.
Researchers found phosphatidylinositol bisphosphate (PIP2) essential for epithelial cell-cell adhesion and maintaining cellular identity. PIP2 regulates epithelial properties by recruiting Par3 to the plasma membrane, facilitating the formation of adherens junctions and preventing epithelial-mesenchymal transformation.
A new low-cost, portable instrument uses photonic technology to detect SARS-CoV-2 in saliva samples with high sensitivity and speed. The device can measure small quantities of virus like PCR tests but is as fast as rapid antigen tests.
Researchers have revealed mechanisms by which polyps develop into colorectal cancer, setting the stage for improved surveillance utilizing precision medicine. The study found that serrated polyps derive from metaplasia, an abnormal change of cells into non-native tissue.
The Iterative Indirect Immunofluorescence Imaging (4i) technique refines standard immunofluorescence imaging by visualizing up to 40 proteins in a single cell. A new computer program, Multiplexed Protein Maps, analyzes the data and generates maps of protein distribution, providing a systematic survey of cellular organization.
A particular protein, DNAJB9, is found in abundance in the kidneys of patients with fibrillary glomerulonephritis, an aggressive kidney disease. The discovery may improve diagnosis and treatment options for this disease, which affects nearly half of patients who become dependent on dialysis.
Researchers at MIT have developed a new microscopy technique that enables precise visualization of RNA molecules in intact tissues. By expanding the tissue sample before imaging, they can obtain high-resolution images of RNA distribution using ordinary microscopes. This breakthrough could help scientists study gene expression and disea...
The Parsortix system demonstrated comparable speed in capturing CTCs for prostate cancer compared to existing methods, while also showing improved capture of CTCs responsible for metastatic cancer. The technology offers a promising approach to detect and treat metastatic cancer.
A new approach to tag proteins has been developed using plant protein epitopes, inntags, which are stable and do not compromise native function. The smallest protein domains with strong structural determinants were selected for tagging purposes.
A recent study has identified a previously unknown cell type in the urethra of mice, which expresses cholinergic receptors and may serve as sentinels to detect hazardous substances. This finding could provide insight into cellular interaction and defensive measures against pathogens, potentially preventing urinary tract infections.
The novel peptide LifeAct allows for the visualization of actin in living cells, facilitating research into various diseases. This breakthrough technology has the potential to improve our understanding of actin's role in fundamental processes and its involvement in diseases such as polycystic kidney disease and invasive tumors.