Articles tagged with Nucleases
Researchers have identified a novel CRISPR mechanism, Cas12a3, that specifically targets transfer ribonucleic acids (tRNA) in bacteria. This discovery provides new insights into the immune response of bacteria and has potential applications for molecular diagnostics and other technologies.
Researchers discovered that the functional splitting of transposon-derived RNAs drove the emergence of Type V CRISPR-Cas immunity. This innovation enabled the development of compact nucleases with flexible guide RNAs, offering design principles to create smaller and more versatile CRISPR tools.
A team of researchers has identified the USP50 protein's role in regulating DNA replication by deciding which enzymes to use during critical processes. The study found that USP50 helps cells balance nuclease and helicase activity, preventing replication defects when it is absent.
Researchers discovered that Streptomyces davawensis produces virus-like particles facilitating host reproduction. The particles contain an enzyme degrading genomic DNA, allowing for extracellular DNA release and scaffold creation. This finding reveals the exploitation mechanism of virus-related nanoparticles for bacterial proliferation.
A new tool called Subak, made from silver nanoclusters, can detect nuclease digestion at lower costs than traditional methods. This innovation could strengthen diagnostics and make diseases easier to detect.
Researchers developed Subak, a cost-effective method to detect nuclease digestion using fluorescent silver nanoclusters. The tool reduces costs of nucleic acid detection tests, such as those used for COVID-19 identification.
Researchers at Linköping University develop a new method to deliver strong compounds specifically to bacteria, allowing for efficient and safe treatment of infections. The TOUCAN strategy uses nucleases to target bacterial DNA, reducing side effects associated with current antibiotics.
Researchers have discovered a surprising mechanism by which the molecular machine Dis3L2 unwinds and destroys RNA molecules. By changing shape, Dis3L2 reveals an RNA-splitting wedge, allowing it to execute its tasks in a more dynamic and versatile way.
Scientists have identified a unique CRISPR nuclease that not only recognizes and cleaves viral RNA but also damages other RNA and DNA inside the cell, impairing bacterial growth. This discovery opens up new possibilities for molecular diagnostics and direct detection of RNA biomarkers.
A recent study has unveiled how nucleotide excision repair (NER) is controlled at the molecular level, shedding light on its role in cancer treatment. The research revealed that TFIIH uses XPG to stimulate motor activity and locate damaged DNA, licensing XPG nuclease activity to excise it.
Researchers have discovered two new, compact Cas9 nucleases that can work in human cells and may expand the toolbox for genome editing. The new nucleases have relatively short PAMs, making them suitable for delivery via adeno-associated viral vectors.
Researchers have developed a new CRISPR-Cas9 variant that reduces unintended changes in DNA, increasing precision in gene therapy. The SaCas9-HF variant shows high on-target efficiency and nearly undetectable off-target activity, offering a promising alternative for precise genome editing.
Researchers have elucidated the complete three-dimensional structure of the MR complex, a molecular machine responsible for detecting and repairing DNA damage. The new structure reveals how the complex binds to DNA and initiates repair processes, shedding light on the intricate mechanisms involved.
DNA nanotubes designed by Yi Li and Rebecca Schulman can heal themselves in serum, extending lifetimes from 24 hours to over 96 hours. The researchers developed a self-repair process using smaller DNA tiles that repair damaged structures by replacing or joining to the nanotube ends.
Researchers at Saint Louis University have discovered a new defense mechanism in BRCA-deficient cancer cells that allows them to survive and thrive despite chemotherapy drugs. The study reveals the role of nucleases in degrading DNA replication forks, leading to increased chemotherapy sensitivity.
A team of scientists has visualized the dynamics of the CRISPR-Cas9 complex using high-speed atomic force microscopy. The study provides unprecedented insights into the CRISPR-Cas9-mediated DNA cleavage mechanism, highlighting its potential for gene editing.
A team of researchers has successfully developed a genome editing technique that induces target point mutation without cutting the DNA. This technique offers high-level editing operations with reduced cytotoxicity, making it suitable for gene therapy, disease research, and organism breeding.
A new Cas9 variant, SpCas9-HF1, eliminates unwanted DNA breaks and reduces them to undetectable levels, expanding therapeutic applications. The high-fidelity variant is also important for research applications where off-target effects can confound results.
Researchers at Harvard Medical School and Massachusetts General Hospital developed two new strategies to reduce off-target effects of CRISPR/Cas9 genome editing. These techniques use truncated guide RNA molecules and the addition of a FokI domain to the Cas9 protein, resulting in highly specific genome editing.
Researchers at Massachusetts General Hospital have engineered a new version of the gene-editing tool CRISPR-Cas9, enabling it to target an expanded range of DNA sequences. The new variants show improved specificity, reducing off-target mutations and expanding the potential applications for CRISPR technology.
A novel computational assisted design strategy was introduced to lower the complexity of ZFN production. The FoldX force field-based approach predicts protein-DNA binding energy, reducing failure rates and increasing efficiency in producing customized ZFNs.
Scientists at Duke University Medical Center have determined the structure of a nuclease that will help understand several DNA repair pathways. This discovery is important for understanding mismatch repair pathway and other pathways related to cancer biology and aging.