Articles tagged with Sanger Sequencing
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Genalive wins largest outsourced testing service contract in Saudi Arabia's history, covering 83 public hospitals and multiple genomics methodologies. The deal will enhance local precision medicine services with comprehensive solutions for whole genome sequencing, exome sequencing, and more.
A novel methodology called qSanger can easily quantify DNA and identify genetic variations in cultured bacteria, offering a cost-effective alternative to traditional methods. The approach uses amplitude ratios of aligned electropherogram peaks from mixed Sanger sequencing reads to measure plasmid DNA ratios.
A new study introduces a commercially available test that enables laboratories with real-time PCR capabilities to detect known SARS-CoV-2 variants. The assay is faster and more cost-effective than traditional sequencing methods, allowing for more widespread tracking of circulating variants.
A new study published in Applications in Plant Sciences highlights the negative effects of clearcutting on mycorrhizal fungi, showing less diversity in formerly deforested areas. High-throughput sequencing reveals over 300 distinct fungal lineages in soil and root samples, shedding light on ecosystem health.
Researchers at the Wellcome Sanger Institute and Pacific Biosciences successfully assembled the genetic code of a single Anopheles coluzzii mosquito, opening doors to understanding genetic diversity in insects. The breakthrough reduces DNA needed for genome sequencing by an order of magnitude, enabling studies on previously inaccessibl...
The Wellcome Sanger Institute has completed sequencing the genomes of 25 UK species, enabling research into their biodiversity and potential for conservation. The newly-sequestered genomes will shed light on various biological phenomena, such as brown trout migration patterns and robin magneto receptors.
The Wellcome Trust Sanger Institute has sequenced 25 new genomes of UK species, including Grey Squirrels and European Robins. The project aims to understand the biodiversity of the UK and aid conservation efforts.
The Wellcome Trust Sanger Institute is sequencing 25 new UK species genomes to aid conservation and understand biodiversity. The results will be made publicly available and lead to future studies on the UK's rich species richness.
Researchers identified eight functional variant mutations in the ADCY9 gene associated with non-syndromic oral clefts (nsCL/P) in Puerto Rican children. The study found three rare missense mutations, including rs52791170/K564Q and rs372048350/A811V, which were not previously reported in Puerto Ricans.
Scientists have developed a new gene technique to find disease-causing mutations in patients with brain malformations. The technique uses next-generation sequencing technology to sequence hundreds of copies of genes in a panel of candidate genes, identifying somatic mutations that were previously undetectable.
A new diagnostic test for detecting BRCA1 and BRCA2 mutations has been developed using second-generation sequencing technology. The test is as sensitive as standard methodology but has the potential to improve efficiency and productivity in genetic testing laboratories.
The researchers validated 1,799 sites and predicted an additional 1,782 sites, resulting in a combined list of 3,581 accurate sites. The team gained insights into the model organism's fundamental biology, including patterns of editing and alternative splicing.
Researchers sequenced DNA molecules directly without library preparation, using less than one nanogram of DNA. The technique has potential for fast and efficient identification of organisms in hospitals and healthcare settings.
A novel coronavirus most closely related to viruses found in bats has been identified, prompting concern over potential human transmission. The virus is believed to have originated from an animal source, with similarities to viruses isolated from Asian and European bat species.
Researchers successfully used exome sequencing to diagnose genetic diseases in patients with intellectual disability, blindness, deafness, movement disorders, cancer, and OXPHOS diseases. The technique was able to identify causative mutations in up to 20% of cases, offering a more efficient alternative to traditional Sanger sequencing.
Using the foxtail millet genome as a reference, researchers have developed genetic tools for switchgrass, a promising biofuels feedstock. The high-quality genome allows for efficient transformation methods and understanding of adaptation mechanisms, making it an ideal model system for studying grasses.
A new web-based application, Pyromaker, has been developed to accurately identify complex genetic mutations. The tool uses simulated pyrograms to generate a virtual trace of the expected signal, allowing for clearer interpretation of ambiguous results from current testing methods.
Rebekah Stackpole Zimmerman received the Richard King Trainee Award for her manuscript on a custom resequencing array for Dilated Cardiomyopathy, published in Genetics in Medicine. The study validated the use of this array to sequence 19 genes associated with DCM, improving test cost and turnaround time by approximately 50%.
The European Nucleotide Archive (ENA) consolidates three major sequence resources, providing free access to over 20 terabases of nucleotide sequence data. The ENA offers improved submission and data-access tools, making it easier for users to share their sequence data.
This study sequenced and assembled nine BACs of giant panda, filling gaps in genomic knowledge and challenging the perception that it's a species at an evolutionary dead end. The data suggests that conservation strategies should focus on restoring wild habitats and maintaining regional genetic diversity.
Geneticists propose six labels for genome sequence data to estimate quality, ranging from standard draft to finished sequence. This could aid in developing vaccines more efficiently and responding to emergencies.
NGS technologies offer streamlined workflows, massive parallelism, and cost reduction through targeted sequencing of specific genes or regions. Researchers are applying NGS to various fields, including hereditary cancer research and bacterial gene expression studies.
The Wellcome Trust Sanger Institute has sequenced 300 human genomes in six months, producing over 1 trillion letters of genetic code. This data will revolutionize human medical genetics and allow researchers to answer questions previously unthinkable.
A new hybrid method combines the best of old and new genome-sequencing technologies to produce better quality genomic information. The approach evaluates the utility and cost-effectiveness of two sequencing methods and finds that a hybrid method produces superior results.
The Illinois pig project will sequence 2.5 billion chemical base pairs at the Wellcome Trust Sanger Institute in the UK, revealing similarities with the human genome. The completed swine genome is expected to lead to advancements in biomedicine, including transplants and disease treatments.
Scientists have sequenced the 'CT18' strain of Salmonella typhi, resistant to cheap antibiotics, and developed new treatment methods. The research provides crucial information on disease spread and mechanisms, potentially leading to a vaccine that can be affordable for everyone.