Articles tagged with Quantitative Polymerase Chain Reaction
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Researchers found that digital PCR for BCR::ABL1 is more sensitive and accurate than conventional real-time quantitative PCR to detect ultralow levels of residual leukemic disease. The technology enabled the detection of stable deep molecular remission in 97% of patients, allowing for safe discontinuation of treatment.
A breakthrough CRISPR-based test has been developed to diagnose Pneumocystis jirovecii pneumonia (PJP), a life-threatening fungal infection. The test detects RNA from live fungi in blood samples and throat swabs, providing a faster and more accurate diagnosis than current invasive bronchoscopy procedures.
Researchers found significantly higher levels of IL-18 expression in osteoarthritis patients and cells compared to healthy controls. IL-17 promoted IL-18 production through the MEK/ERK/miR-4492 axis, indicating potential therapeutic targets for OA treatment.
A novel droplet digital PCR assay detects KMT2A fusion markers in AML patients, enabling sensitive MRD detection and guiding treatment decisions. This breakthrough may improve patient outcomes by assessing response to therapy and long-term surveillance.
A new device using dielectrophoresis selectively isolates Mycobacterium tuberculosis from sputum samples, providing a purified sample for molecular confirmation. The technology has shown promising results in detecting TB in underserved areas, with high concordance rates compared to culture diagnosis.
Researchers found SARS-CoV-2 in 18.2% of tear samples from patients with COVID-19, and in 12.1% of those collected using the Schirmer strip test. The method may predict patient prognosis and provide an alternative to uncomfortable nasopharyngeal swabbing.
Researchers explore the emergence of antibiotic resistance genes in bioaerosols, including sources and detection strategies. The study highlights the need for monitoring and understanding the relationship between human, animal, and environmental microbiomes to counter the spread of AR.
Researchers found that sewer slime can accumulate SARS-CoV-2 RNA, which could decompose or slough off later. The slime's ability to hold onto the viral RNA was linked to the number of diagnosed COVID-19 cases in late fall. Further studies are needed to assess its impact on wastewater epidemiology.
Scientists from Japan and USA develop a microfluidic device for purification of tuberculosis genomic DNA fragments, enabling accurate diagnosis of diseases. The device uses transient ITP and electrokinetic trapping to detect and purify small cfDNA fragments.
Researchers have developed new qPCR assays to quantify the transmission of antimicrobial resistance (AMR) using water and wastewater samples. These methods provide a more exact way to determine AMR by measuring DNA in wastewater samples.
A new study suggests that asymptomatic individuals play a significant role in the transmission of pertussis, with up to 70% of infected mothers displaying no symptoms. The findings challenge traditional approaches to diagnosing and treating patients, highlighting the need for a more nuanced interpretation of qPCR test results.
A new laboratory method called TaqMan Array Card (TAC) has been validated for detecting a diverse range of bacteria that cause diarrheal disease. The TAC is faster and cheaper than traditional methods while delivering comparable results, enabling comprehensive monitoring of enteropathogens in informal settlements.
A reliable COVID-19 test developed by Anglia Ruskin University professor Stephen Bustin could reduce virus spread by cutting false negatives and delivering faster results. The Cov2-ID test targets three viral genes, achieving 100% accurate results from nearly 30 patient samples.
A novel epigenetic diagnostic approach enables early detection of primary immune deficiencies in newborns, including Severe Combined Immunodeficiencies and X-linked agammaglobulinemia. The technology also shows promise for improving HIV treatment in low-resource countries by accurately monitoring immune cell counts on dried blood samples.
The study reveals a tendency for duplication in the 9p21.3 sequence associated with coronary artery disease, which may affect predisposition to CAD. Sequence analysis shows varied repeats with 10-15 SNPs between each other and 82 SNPs between the human genome sequence.
A multicenter study of 21 laboratories demonstrated digital PCR's high reproducibility in quantifying cancer mutations across different locations. The method showed greater reproducibility and reduced variability compared to real-time quantitative PCR.
Scientists from Granada and Valencia universities developed a new molecular method to detect Toxoplasma gondii in ham, finding the parasite's prevalence varied between 0% and 32.35%, with some samples being infective. The study suggests freezing ham before curing can eliminate the parasite faster.
Arizona State University is developing a diagnostic test to measure absorbed radiation in individuals during a nuclear incident. The ASU Radiation Biodosimetry System (ARad) uses real-time quantitative PCR-based technology to estimate absorbed radiation.
A new DNA-based digital PCR assay has been developed to detect persistent disease in CML patients who appear to be in remission. The assay was found to be more sensitive than current methods, detecting disease in 81% of samples, and may lead to improved treatment decisions for individual patients.
A new diagnostic device called DOTS qPCR may significantly reduce the time needed to diagnose tissue infections. It uses a novel approach to molecular diagnostics that is faster, more efficient, and less expensive than current alternatives.
A new measurement method using droplet digital polymerase chain reaction (ddPCR) estimates the distribution of aquatic animals in water by quantifying target DNA copies. This technique offers higher accuracy than existing methods, especially at low environmental DNA concentrations.
A study in The Journal of Molecular Diagnostics found a combination of nucleic acid sequence-based amplification and real-time quantitative PCR detects aspergillosis with 100% accuracy. This diagnostic test has the potential to improve patient outcomes by providing early detection of this potentially deadly fungus.
Researchers have created a new test to quickly identify snakes infected with Ophidiomyces ophiodiicola, a fungus killing snakes in the Midwest and eastern US. The test allows for early intervention and potential treatment of infected snakes.
A study published in Nature Methods demonstrates Droplet Digital PCR's ability to precisely and reproducibly quantify microRNA in plasma and serum across different days. This technology can improve the development of miRNA-based blood-based biomarkers, such as those for cancer diagnosis.
A PLOS ONE study has confirmed that Droplet Digital PCR (ddPCR) technology is suitable for routine analysis of genetically modified organisms (GMOs) in food, feed, and seeds. The study found that ddPCR is more accurate and reliable than real-time quantitative PCR (qPCR) for quantifying GMOs, especially at low levels.
Mercyhurst research delivers near real-time water quality results, reducing exposure to potentially hazardous bacteria. The new system uses computer predictions and qPCR technology to detect E. coli contamination in two hours.
Advances in qPCR technology are expanding its applications in basic research and diagnostics, enabling more efficient detection and quantification of specific DNA sequences. Companies like Qiagen and Bio-Rad Laboratories are developing new methods to improve multiplex PCR and sample preparation.