Articles tagged with Rna Polymerases
Researchers at Colorado State University have designed a genetic modification that strips the ability of coxsackievirus B3 to replicate and cause disease. By changing a specific amino acid in the RNA polymerase enzyme, they aim to create a live-attenuated vaccine to protect against this virus and others like it.
The Rutgers scientists reveal how a transcription activator protein interacts with RNA polymerase and DNA to initiate transcription. The discovery provides a molecular picture of transcription activation at a target promoter.
The study provides unprecedented molecular views of the critical early events in gene expression, positioning RNA polymerase to start transcription. The researchers captured human PICs in three functional states, revealing new structural elements and insights into DNA engagement, promoter melting, and transcription bubble stabilization.
Researchers at HKUST elucidated the dynamics of backtracking in RNA polymerase II, revealing a stepwise process that detects mis-incorporated RNA and corrects errors. The study provides insight into fundamental mechanisms of transcription and may help understand human diseases and aging related to transcription infidelity.
The CHOP/Rutgers team developed new experimental approaches to detect DNA scrunching during transcription start site selection. They identified the TSS, front and rear positions where RNA polymerase attached to DNA, and found that as the position of the TSS changes, the position of the enzyme's leading edge changes in lock step.
Researchers at Helmholtz Zentrum München developed a method to analyze protein modifications, including the phosphorylation of RNA polymerase II. This helps regulate gene expression by controlling enzyme activity at precise sites.
Scientists at Rockefeller University identified a new regulator of RNA polymerase II, a critical process for gene expression. The discovery could lead to more specific cancer therapies by targeting this form of gene regulation.
A new crucial gene, POLR1C, has been identified as the cause of nearly 10% of cases with 4H leukodystrophy, a common form of the disease. This discovery sheds light on the molecular mechanism behind the disease and may lead to new diagnostic tools and therapeutic options.
Researchers at Gilead Sciences and Beryllium published structural data on HCV polymerase during RNA replication, shedding light on the NS5B catalytic mechanism. This information will be useful in identifying replication inhibitors of other pathogenic viruses in the Flaviviridae family.
A study by University of Utah researchers found a unique mechanism used by the Ebola virus and other related viruses to replicate. The discovery, which was made possible through computer simulations, may lead to new targets for antiviral drugs within five to 10 years.
Scientists have determined the complete structure of the influenza virus polymerase, a key machine that makes copies of the virus' genetic material and reads out instructions. The high-resolution structure reveals how the polymerase works as a whole, providing new insights into its function and potential targets for drug design.
Researchers at NYU Langone Health discover how RNA polymerase patrols the genome for DNA damage and recruits partners to repair it, leading to fewer mutations and less disease. The study's findings have major implications for our understanding of DNA repair and its role in cancer and aging.
Researchers at EMBL have determined the 3D structure of RNA polymerase I, revealing a unique 'Swiss-army knife' strategy that allows it to produce RNA molecules faster than its counterpart, RNA polymerase II. The protein's larger size and efficiency are due to its built-in modules, which prevent the need for external recruitment.
Researchers at Ludwig-Maximilians-Universität München have solved the structure of RNA polymerase I, a crucial enzyme in cell growth. The study reveals details on how the enzyme regulates protein synthesis and provides potential targets for cancer treatment.
A new study reveals that DNA sequences at the beginning of genes in fruit flies contain complex instructions for RNA polymerases to read and transcribe essential genes. The findings suggest that these instructions play a crucial role in regulating gene expression during early embryonic development.
A new paper in Cell Reports found that paused RNA polymerase plays a crucial role in regulating gene expression during embryonic development. The study revealed that the paused state is regulated over time rather than by tissue type, and that proteins called Polycomb group help keep it in check.
Researchers at Scripps Research Institute describe the structure and function of influenza's ribonucleoprotein (RNP) complex, which is essential for viral replication. The study provides new insights into the flu virus's vulnerabilities, including a shape-change during gene transcription and key interactions between proteins.
Researchers have unraveled the mystery of a unique virus-coping mechanism, revealing a crucial role for motif D in accurately replicating genetic material. This discovery holds promise for improving existing vaccines and designing new ones against RNA viruses that eluded vaccination strategies.
Researchers have discovered that six unnatural nucleic acid polymers can share information with DNA, shedding light on the origins of life. The discovery opens up practical applications for molecular medicine, including new diagnostics and therapeutics.
Indiana University researchers have discovered that specific types of RNA polymerase enzymes differ in function based on variation in their protein subunits. This finding reveals the importance of subunit composition in selecting which genes are silenced or expressed, with implications for understanding genetic disorders and diseases.
A team of IRCM researchers confirmed the universality of essential cellular process phosphorylation of RNA polymerase II, contradicting recent genome-wide analyses. The study provides critical insights into genetic regulation and may lead to new treatments for genetic diseases and cancer.
Stowers researchers find that each class of genes transcribed by RNA polymerase II has a specific class of elongation factors, controlling which genes are transcriptionally regulated. This discovery adds a new dimension to transcriptional elongation control and has significant implications for understanding gene expression.
Researchers have determined the architecture of the mitochondrial RNA polymerase, revealing its molecular copy machine mechanism. The discovery provides new insights into the evolution of mitochondria and their genome, shedding light on how they produce energy for cells.
Researchers at NYU Langone Health have discovered the cellular mechanisms that generate chromosomal breaks in bacteria. They found that collisions between major gene expression lead to chromosomal breaks, which may explain stress-driven evolution in bacteria and certain human diseases.
Researchers at Ohio State University identified a family of proteins that close a critical gap in RNA polymerase, enabling it to maintain its grip on DNA and activate genes. This discovery has implications for antibiotic development and could contribute to understanding gene expression in all living organisms.
Researchers have discovered that MED26 acts as a go-between linking transcriptional complexes, recruiting elongation factors to DNA to jump-start stalled polymerase. This discovery sheds light on the regulation of gene expression and suggests new strategies for regulating gene expression in healthy and diseased cells.
Researchers at Boston University School of Medicine have identified a novel mechanism for controlling gene expression, which is evolutionarily conserved in humans. This process, called transcriptional attenuation, involves the blocking of premature termination complexes to allow genes to be expressed under certain stress conditions.
Researchers at University of Maryland provide new insight into the initiation phase of bacterial gene transcription, showing a three-step process involving RNA polymerase and DNA bending. The study confirms experimental observations and establishes an active role for RNA polymerase in the process.
Researchers at NYU Langone Medical Center have discovered a new paradigm to understand the molecular principles of gene transcription in bacteria. The study reveals an allosteric mechanism by which Rho terminates transcription and shows that Rho binds tightly to RNA polymerase throughout the transcription cycle.
Researchers at Ludwig-Maximilians-Universität München have detailed the process of RNA polymerase II initiating gene transcription. The complex recognizes signals in the DNA sequence and uses TFIIB to bind to the TATA box, producing a sharp kink in the DNA.
A team of researchers at the University of Leeds has developed a model that explains how errors are corrected during protein synthesis. The study suggests that a molecular machine called RNA polymerase uses a unique mechanism to remove incorrect letters from the growing RNA chain, allowing copying to resume.
Researchers at Helmholtz Zentrum München have identified the enzyme TFIIH kinase as responsible for selectively phosphorylating RNA polymerase II, regulating the production of specific RNA molecules. This discovery sheds light on epigenetic mechanisms and their significance in cancer and disease.
Researchers have developed a new crosslinking method called TRAP to study protein interactions in living cells. The method uses small crosslinkers that can be controlled with light to identify proteins working together, revealing new details about RNA polymerase in bacteria.
Biologists at Washington University in St. Louis have found that plant enzymes Pol IV and V are specialized forms of RNA Polymerase II, essential for decoding genetic information in eukaryotes. The discovery sheds light on the evolution of RNA polymerases and their role in gene silencing.
A team led by Craig Pikaard discovered a new mechanism by which plant cells silence potentially harmful genes, involving the non-coding region of DNA and two plant-specific RNA polymerases. The research has major implications for gene therapy, where RNA-centric approaches show promise for controlling diseases such as cancer and HIV.
Researchers have uncovered the mechanism by which specialized activator proteins kickstart the RNA polymerase machine, allowing genes to be activated at specific times. This process is crucial for protein production and bacterial adaptation, making it a potential target for developing novel antibacterial compounds.
Researchers have discovered the mechanism behind how a specific antibiotic, myxopyronin, kills bacteria. The study found that the antibiotic binds to RNA polymerase, interfering with its ability to use DNA to start gene expression, effectively creating a road block that halts bacterial growth.
Pol II selects correct NTPs to add to mRNA chains with exquisite precision, using a kinetic selection mechanism that involves the trigger loop. The study reveals how Pol II discriminates against incorrect NTPs and sheds light on the mechanisms of fidelity in cellular genetic copying machines.
Researchers have mapped nucleosome organization along genes in Drosophila melanogaster, revealing a critical stop sign for transcription. This discovery highlights the importance of nucleosomes in regulating gene expression and has implications for developing effective anti-viral drugs against HIV.
Researchers have shed new light on the role of RNA polymerase II in gene expression, revealing a complex mechanism that allows for efficient use of existing genes. The study found that phosphorylation of serine 7 at the carboxyterminal domain is essential for processing and maturation of specific gene products.
A mutation in RNA polymerase III enzyme disrupts organ growth in zebrafish, with specific tissues like the intestine being severely affected. The study provides hope for a therapeutic application against cancer by targeting the enzyme's role in protein production.
Researchers at Albert Einstein College of Medicine measure transcription stages in real-time, revealing inefficient first two stages with only 1% polymerases remaining. Elongation phase takes 517 seconds, while pausing and rapid synthesis may regulate gene expression.
Researchers used powerful imaging techniques to study the atomic level interactions between bacteria and antibiotics, revealing a key enzyme structure that enables gene expression. The findings provide insights into potential new antibiotic designs that can prevent bacterial resistance.
Researchers found that the trigger loop acts like a trap door to close off the active center and form extensive interactions with NTP, ensuring accuracy. This mechanism couples NTP recognition and catalysis, explaining the high fidelity of transcription.
A novel mechanism of dengue virus replication has been discovered, involving the circularization of its genome. This process allows the viral RNA polymerase to interact with a distant site on the genome, initiating replication. The study's findings suggest a widespread strategy for viral RNA replication.
Scientists from the University of Wisconsin-Madison have made a fundamental discovery about RNA synthesis in E. coli, shedding light on how bacteria regulate gene expression. The study found that a specific region within DNA promoters makes contact with RNA polymerase, but not at promoters for genes involved in ribosomal RNA production.
Researchers at Cold Spring Harbor Laboratory have identified a new mechanism for gene regulation known as 'gene loops', which play a crucial role in controlling the expression of genes. This discovery has significant implications for our understanding of gene function and regulation.
Researchers at Lawrence Livermore National Laboratory used fluorescence resonance energy transfer (FRET) to study the transcription of genes in DNA. They found that the initial and final stages of this process occur simultaneously, contradicting earlier theories that proposed separate processes for these stages.
A fourth kind of RNA polymerase, Pol IV, has been found in plants, playing a crucial role in maintaining genome integrity. It helps direct DNA methylation to specific sequences, ensuring proper gene expression and preventing developmental problems. The discovery sheds light on the unique features of plant biology.
Researchers at Stanford Medicine have made groundbreaking discoveries about the structure of RNA polymerase, a crucial enzyme in gene expression. The team's findings reveal intricate details about the enzyme's interactions with helper molecules and DNA, providing a deeper understanding of transcription and protein production.
A new class of compounds called CBR703 series inhibit RNA polymerase, a key enzyme in gene expression, and hinder the ability of bacteria to perform crucial catalytic functions. The compounds render RNA polymerase useless by binding to a specific place on the enzyme.
Cumbre Inc. and University of Wisconsin-Madison researchers have published data on a new class of bacterial RNA polymerase inhibitors with major breakthrough potential, offering a powerful tool to study gene expression mechanisms and developing new antibiotics against bacterial pathogens.
Researchers at Cornell University have confirmed a theory about how a protein complex known as FACT helps cells read their genetic code. By studying the activation of a heat-shock gene in fruit fly cells, they found that FACT and other proteins quickly move to chromosomal sites where transcription occurs.
Rockefeller University scientists have discovered how transcription begins in bacteria, a crucial step for developing new antibiotics. The structure of the RNA polymerase holoenzyme reveals a novel protein-protein interaction that regulates transcription initiation.
Researchers found protein Nrd1 helps RNA polymerase II recognize 'stop sign' for certain genes. This mechanism may help understand HIV's hijacking process and fundamental mechanisms of regulating gene expression in yeast.