Articles tagged with Polymerase Chain Reaction
A study published by PCR found that TAVI significantly lowers the incidence of early stroke at 30 days and two years compared to surgical aortic valve replacement. The procedure also showed similar one-year mortality rates for both groups with stroke or encephalopathy at 30 days.
A large dataset reports that deferring revascularization in patients with intermediate stenosis leads to fewer interventions. The rate of major adverse cardiovascular events (MACE) was low, especially in those without acute coronary syndromes.
The TOPIC study found that switching from a new P2Y12 inhibitor plus aspirin to maintenance DAPT with aspirin plus clopidogrel one month after acute coronary syndrome (ACS) significantly reduced bleeding risk, with a 70% lower rate of major bleeding events. Ischemic events remained similar between the two regimens.
A study published in JAMA Surgery found that certain breast cancer patients who achieve pathologic complete response (pCR) after chemotherapy have a low risk of developing local metastases, making them candidates for less invasive treatment options. These patients may be able to avoid follow-up surgery, including axillary surgery.
CSU researchers create affordable and rapid testing systems for water and food-borne pathogens, aiming to reduce expensive follow-up tests. The new system uses enzymes associated with fecal indicator bacteria, detecting harmful bacteria within four to 12 hours.
A new digital assay using circulating tumor cells (CTCs) has been developed to detect liver cancer, showing high specificity and sensitivity. The test can identify HCC in patients with a positive score, even when other methods like AFP screening are negative.
Adaptive PCR, a new method developed by Vanderbilt University biomedical engineers, uses left-handed DNA to monitor and control PCR reactions. This approach promises to simplify PCR operation, improve reliability, reduce sensitivity to environmental conditions, and enable handheld size.
A new open-access database compiled by Korean scientists provides comprehensive PCR primers for detecting and identifying RNA viruses. The MTPrimerV database contains 152,380 primer pairs covering 1,818 viruses, with an accuracy rate of 100% against the latest NCBI RefSeq database.
Researchers create technique to identify pairs of short DNA sequences from beef, buffalo, and pork in hot dogs, providing a more reliable approach than existing methods. The study found that all 'beef' hot dogs contained buffalo meat, highlighting the need for accurate food testing.
Cardiologists from 115 countries gained knowledge and skills in treating cardiovascular disease through live demonstrations and educational sessions. They learned about the latest developments and best practices in coronary interventions, valvular disease, heart failure, peripheral interventions, and more.
The OPINION study shows that OFDI-guided PCI achieves equivalent clinical and angiographic outcomes to IVUS-guided PCI at 12 months. The study results contribute to defining the clinical value of OCT guidance in PCI.
A study at EuroPCR 2016 found that half of transcatheter heart valves may undergo degeneration within 10 years after TAVI. The study, which followed 704 patients for up to 10 years, identified 35 cases of valve degeneration, with approximately two-thirds associated with intravalvular regurgitation.
The conference features research on mitral and tricuspid valve interventions, new devices for percutaneous coronary interventions, and approaches to treat heart failure and stroke. Key findings highlight the potential of TAVI for low-risk patients and the impact of recent trials on clinical practice.
DZIF scientists have developed optimized assays and a control for quantifying viruses in blood and urine, improving the reliability of current tests. The new tests are more sensitive to low viral concentrations and comparable across different strains.
New techniques and devices are being developed to treat heart failure, mitral valve regurgitation, and coronary artery lesions. Studies show high procedural success rates and improvements in heart function and quality of life.
A new study has demonstrated a molecular method that reduces the wait time to confirm transgenic work from weeks to just a few days. Digital drop PCR (ddPCR) was found to be reliable, fast and high throughput, making it a promising alternative to traditional methods.
Patients with stage 2 or 3 triple-negative breast cancer (TNBC) who achieved a pathologic complete response (pCR) after presurgery chemotherapy had significantly improved event-free and overall survival compared to those with more than minimal residual invasive disease. This benefit was observed in patients with larger tumors or lymph ...
Adding carboplatin to presurgery chemotherapy significantly improved disease-free survival rates for patients with triple-negative breast cancer (TNBC). The study found that patients with pathologic complete responses had the lowest risk of relapse, supporting the use of carboplatin as a standard part of neoadjuvant therapy regimens.
A team at University of California - Santa Cruz developed chip-based technology for reliable Ebola detection, combining microfluidic and optofluidic chips. The system achieved comparable sensitivity to PCR analysis with excellent specificity.
A new technology developed by UC Berkeley researchers uses light to accelerate the heating and cooling of genetic samples, greatly expanding clinical and research applications. Results can now be obtained in minutes instead of hours, revolutionizing DNA diagnostics.
A new method using Droplet Digital PCR Technology enables rapid determination of chromosomal phase of allelic markers hundreds of kilobases apart, providing insights into disease cause and severity. This technology overcomes challenges of current phasing methods, allowing researchers to quickly and affordably determine haplotype data.
EuroPCR 2015 highlighted the benefits of mechanical thrombectomy in acute stroke, showing significant improvement in outcomes for one-third of patients who qualify for the procedure. The meeting also showcased advancements in coronary interventions, including chronic total occlusions and stent thrombosis.
A recent study presented at EuroPCR 2015 found that thickening of valve leaflets following TAVI or surgical aortic valve implantation is relatively rare and not linked to short-term clinical events. The study suggests that current TAVI and TAVR programs do not need modification, but further research is needed.
A large registry study found that patients experiencing stent thrombosis between 2-30 days post-procedure had higher mortality rates compared to those who developed the complication within 24 hours. The timing of stent thrombosis may be linked to more severe infarction and long-term heart failure.
Dr. Ferdinand Kiemeneij and Dr. Shigeru Saito received the Ethica Award for their roles in developing and teaching the radial approach to coronary catheterization. They are internationally renowned for their contributions to interventional medicine, patient comfort, and quality of life.
A recent study presented at EuroPCR 2015 found that routine thrombectomy during angioplasty for acute myocardial infarction may increase the risk of stroke. The TOTAL trial showed a significant increase in stroke rates within 30 days, with the highest risk period being within 48 hours.
The European Clinical Consensus Conference concludes that research into renal denervation for high blood pressure should not be abandoned until high-quality research is completed according to agreed-upon standards. The group also emphasizes the need for standardization in treatments, populations, methods, and adherence measures.
Recent clinical trials demonstrate significant improvements in neurological function with clot retrieval therapies, increasing recovery rates to 40-50%. However, implementation into practice requires concentrated effort from multiple parties, including training and education.
A new study found that TAVI is safe and effective as a surgical alternative for selected lower-risk patients, with comparable outcomes at two years. The Nordic Aortic Valve Intervention (NOTION) trial showed no significant difference in mortality rates between TAVI and surgical valve replacement.
The 'Radial Paradox' reveals that radial access may offset vascular complications when used as a backup method for femoral access. A study found that non-culprit vessels with significant stenosis are not functionally important, increasing debate on intervention strategy. Meanwhile, elderly patients benefit from drug-eluting stents with...
The German Breast Group study found nab-paclitaxel (ABRAXANE) demonstrates significant benefit for patients with early high-risk breast cancer when compared to conventional solvent-based paclitaxel. Nab-paclitaxel achieved a 9% absolute improvement in pathological complete response rate, outperforming traditional chemotherapy.
The SYMPLICITY-HTN-3 clinical trial confirmed the safety of renal denervation, but questions remain about its efficacy in patients with difficult-to-control hypertension. Further research is needed to identify the best patients who will benefit from treatment with the procedure.
The ACCOAST trial data showed that pre-treatment with prasugrel in NSTEMI patients undergoing PCI was inferior to treatment after angiography, but the results did not change practitioners' current practice. The new session format aims to translate clinical trial science into daily practice.
Experts discussed bioresorbable vascular scaffolds' potential to restore normal vasofunction and promote vascular healing. However, concerns remain regarding acute thrombogenicity, delayed endothelialisation, and uncertainty around long-term safety data.
Leadless pacemakers with seven-to-ten-year battery life have shown significant advancements in reliability and reduced risk of infection and thromboembolic complications. Transcatheter aortic heart valve developments also showcased improved outcomes with no reported cases of severe paravalvular leak.
The EuroPCR panel stresses the importance of personalizing revascularization strategies for STEMI patients. Primary percutaneous coronary intervention (PCI) is considered the gold standard, but its availability can be limited in resource-scarce environments, making thrombolysis a viable alternative.
The first PCR Innovation Day brought together key stakeholders to address unmet clinical needs in interventional cardiovascular medicine. This event provided an opportunity for physicians, inventors, and entrepreneurs to network and discuss innovative technologies.
Researchers found Gulf War veterans with impaired mitochondrial function had significantly delayed phosphocreatine recovery after exercise, a robust marker of mitochondrial dysfunction. Impaired mitochondrial function accounts for Gulf War illness symptoms like fatigue, cognitive challenges, muscle problems, and exercise intolerance.
Researchers have developed a new approach using long-range PCR and next-generation sequencing to obtain large phylogenomic data sets, increasing accuracy in reconstructing evolutionary history. This method allows for the amplification of larger DNA fragments, enabling the targeting of specific genomic regions.
Researchers at Fred Hutchinson Cancer Research Center used Droplet Digital PCR to quantify tumor-infiltrating T-lymphocytes (TILs), a subpopulation of immune cells that improve cancer survival. Higher TIL levels correlate positively with patient survival, suggesting an active role in suppressing tumor formation. The study paves the way...
A new protocol developed by researchers at the University of Cincinnati significantly reduces the need for repetitive lab work in microsatellite-based research. By increasing the efficiency of primer testing and allowing for flexible experimentation, this method streamlines the development process, making it quicker and cheaper.
Researchers developed digital PCR technology to detect IDH1 mutation in CSF of patients with brain tumors, improving treatment choice and patient care. The technique has potential for noninvasive biomarker identification and personalized medicine.
A study by Johns Hopkins Medicine reveals that reported plant genetic material entering human blood may be a false positive, contradicting previous research from Nanjing University. The team used advanced techniques to detect microRNAs in macaques' blood and found highly variable results.
A new investigational device, Helio System (TF-FA), was successfully deployed in four patients with high-risk aortic insufficiency. The device helps stabilize the Sapien XT Transcatheter Heart Valve and improves aortic insufficiency from moderate to trivial.
A multicentre Asian registry reported early experience confirming TAVI efficacy and safety in a physically smaller population. Low stroke and mortality rates were observed, with high procedural success rates, supporting the feasibility of TAVI in Asian patients.
Researchers have developed a new sequencing method that reduces bias in measuring biodiversity by avoiding PCR amplification, allowing for accurate estimation of biomass and identification of species. This method was successfully tested on bulk arthropod samples and identified bacteria and insect species with high accuracy.
The novel TP PCR test yields accurate results without unnecessary additional testing, improving diagnosis and prognosis by accurately sizing the genetic abnormality characteristic of HD. The test correctly sized 240 of 246 samples, including those with a wide range of CAG repeats.
Researchers have developed a technique to overcome inhibitory plant compounds, allowing PCR to successfully amplify DNA from problematic plants. The additive TBT-PAR, containing trehalose, bovine serum albumin, and polysorbate-20, enhances PCR for DNA extracted from various plant species, including tropical and temperate species.
Researchers at Rensselaer Polytechnic Institute have developed an ultrasensitive method for detecting sugar molecules, known as Glyco-qPCR. This technique enables a more detailed understanding of cellular functions than genetic or proteomic information can provide, and has the potential to revolutionize the study of glycans.
A new sequencing approach using long-range PCR and Next Generation Sequencing has been developed to provide a more effective method of BRCA1/2 mutational analysis. The method was successfully tested on 12 familial breast cancer patients, identifying all expected variants with high accuracy.
A new test developed by scientists can accurately detect contaminants in marzipan pastes, including cheap substitutes made from soy and peas. The polymerase chain reaction (PCR) method can identify small amounts of almond substitutes as little as 0.1%.
Researchers at Berkeley Lab have developed a PCR-free technique using the PhyloChip to identify the most metabolically active microbes in a sample. This allows for reliable and affordable analysis of microbial communities in various environments.
A new mathematical model evaluates microsatellite genotyping reliability from degraded DNA templates, enabling reliable genetic information from non-invasive samples. The approach reduces false results and optimizes experimental protocols.
Researchers developed a digital polymerase chain reaction (PCR) device that sets dramatic new performance standards in sensitivity and accuracy of sample screening. The device enables the direct counting of single molecules isolated in individual chambers.
A new procedure devised by USDA scientists increases PCR-based method sensitivity for detecting plant disease organisms, improving diagnostic accuracy and reducing economic harm from asymptomatic seed contamination. The technique, called Bio-PCR, enhances detection rates by 100- to 1,000-fold over conventional methods.
A new test for salmonella in poultry and eggs can produce faster and more accurate results than most currently available tests. This could have prevented contaminated eggs from being shipped to stores and kept consumers safe.
A recent study from Stanford University found that DFA testing for H1N1 influenza is unreliable in critically ill patients. The test was more accurate in non-critical cases, with a 26% positive rate in ICU patients compared to an 82% positive rate in non-ICU patients.
A new method for detecting and genetically identifying salmonella from contaminated foods can provide DNA sequencing-like results in hours, rather than days. This could help recognize outbreaks sooner and stop tainted food from being delivered.
Researchers have developed a new lab-on-a-chip test that detects DNA with excellent sensitivity, eliminating the need for amplification, and enabling wider use of DNA testing. The nanogap sensor technology overcomes current limitations of PCR-based tests, making it a faster and more practical alternative.
This article provides optimization strategies for polymerase chain reaction (PCR) and a method for loading individual cells with fluorescent probes via patch pipettes. The techniques are described in detail, including touchdown PCR and hot-start PCR, and their applications in neuroscience research.