Articles tagged with Real Time Polymerase Chain Reaction
Diagnostics.AI has launched the industry's first fully-transparent machine learning platform for clinical real-time PCR diagnostics, delivering algorithmic transparency and per-test auditability. The platform is CE-IVDR certified and backed by over 15 years of experience and millions of successfully processed samples.
A breakthrough CRISPR-based test has been developed to diagnose Pneumocystis jirovecii pneumonia (PJP), a life-threatening fungal infection. The test detects RNA from live fungi in blood samples and throat swabs, providing a faster and more accurate diagnosis than current invasive bronchoscopy procedures.
A novel real-time PCR method has been developed to detect and identify the emerging zoonotic foodborne pathogen E. albertii, which is often misidentified as E. coli. The study found that E. albertii can survive in the human intestinal tract for approximately four weeks.
Research review finds scent dogs can successfully sniff out COVID-19 in symptomatic, pre-symptomatic, and asymptomatic cases, as well as new variants and long COVID. Scent dogs demonstrate similar or better sensitivity and specificity than current gold-standard tests.
Scientists from Okayama University used environmental DNA (eDNA) to survey the distribution and population density of the endangered bitterling fish in Japan. They found that eDNA concentrations vary according to the downstream channel distance, providing a reference for conservation surveys.
Researchers developed a SARS-CoV-2 saliva assay and prototype device combining speed, ease, and high sensitivity. The SLIDE device detects viral RNA in saliva within 45 minutes, comparable to traditional RT-PCR methods.
Researchers at Columbia University and Rover Diagnostics have developed a low-cost, portable platform that provides RT-PCR results in 23 minutes, matching laboratory-based tests. The system uses plasmonic nanoparticles to achieve real-time and multiplexed RT-qPCR on clinical specimens.
Researchers have developed a rapid COVID-19 test that uses molecularly imprinted polymer nanoparticles to detect SARS-CoV-2. The new test is more sensitive and works under extreme conditions than existing antibody-based tests, with preliminary results indicating it can detect a 6,000-times lower amount of the virus.
A new study introduces a commercially available test that enables laboratories with real-time PCR capabilities to detect known SARS-CoV-2 variants. The assay is faster and more cost-effective than traditional sequencing methods, allowing for more widespread tracking of circulating variants.
Genetic testing of saliva samples has been shown to be more quick and sensitive than nasal swab testing for detecting SARS-CoV-2 virus, with implications for improving public acceptance of COVID-19 testing and reducing healthcare worker risks
A new PCR test can quickly identify all SARS-CoV-2 variants in a positive patient sample, providing crucial information for public health professionals and policymakers. The assay has been shown to have high sensitivity and specificity, making it an valuable tool in monitoring emerging strains.
A Rutgers study found that hospital patients with repeated negative RT-PCR tests but exhibiting COVID-19 signs and/or symptoms were half as likely to receive treatment as those who tested positive. The study suggests that clinicians should not solely rely on RT-PCR tests for COVID-19 diagnosis.
Researchers developed a new reagent-free detection technique for SARS-CoV-2 using Raman spectroscopy and machine learning. The method shows an accuracy of 80% in detecting COVID-19 infections from saliva samples, overcoming limitations of RT-PCR testing.
Researchers developed a new personalized test for monitoring cancer recurrence in acute lymphoblastic leukemia (ALL) patients. The MP PCR uses multiple genomic markers to detect disease recurrence sooner, improving treatment strategies and patient stratification.
Researchers have developed a rapid and accurate breathalyzer test that can diagnose COVID-19 in under 5 minutes, identifying asymptomatic carriers. The handheld device uses surface-enhanced Raman scattering sensors to detect volatile organic compounds exhaled by infected individuals.
The CDC found flaws in the assay design and contamination of one component, leading to false positive reactivity in negative controls. A redesign and improved quality control measures have since been implemented.
A new protocol has been developed to identify potential false-positive COVID-19 results, particularly in asymptomatic non-exposed individuals. The method involved retesting all positive results from such patients, revealing 20 out of 288 initial positives to be false positives.
A computational analysis suggests that rapid antigen tests can achieve similar epidemiological outcomes as RT-PCR tests if enough people are tested. The study's findings have implications for policy changes and optimizing testing strategies in lower-income countries.
A computational analysis of COVID-19 tests suggests that using less sensitive rapid antigen tests can achieve similar outcomes as more sensitive RT-PCR tests if a high number of people are tested. This approach could allow governments in lower and middle-income countries to ramp up testing and reduce the number of infections.
A new protocol processing saliva samples with a bead mill homogenizer improves COVID-19 detection rate and eliminates challenges of nasopharyngeal testing, researchers report. Saliva testing is found to be more effective than nasopharyngeal swabs for COVID-19 detection in a study published in The Journal of Molecular Diagnostics.
A new plasmofluidic chip enables fast and accurate PCR tests in under 13 minutes, offering a significant improvement over current RT-PCR methods. The device uses gold nanoislands to rapidly heat and cool samples, reducing testing time without sacrificing amplification efficiency.
A study found that a chest x-ray scoring system can predict patient outcomes, including death, intubation, and chronic renal replacement therapy, in COVID-19 patients with high pretest probability or rapid test results. The scoring system was accurate and reliable, even among junior residents.
Developed by a team of researchers, the nanoPCR technology uses hybrid nanomaterials to detect viral RNA in under 11 minutes while maintaining high accuracy. The technique has been tested with 150 patients, showing zero false negatives and positives, and demonstrates its potential for rapid, decentralized point-of-care diagnosis.
A study examines SARS-CoV-2 RNA test results in patients who recovered from COVID-19 with prior negative results. Researchers found that some patients tested positive for the virus after recovery, highlighting the need for continued monitoring and testing.
Researchers at NYU Abu Dhabi have developed a new three-step testing approach that improves the accuracy of COVID-19 testing, detecting lower viral loads and reducing false-negative rates. The cost-effective technique uses microfluidics technology and can detect SARS-CoV-2 viral loads as low as one copy/microliter.
A faster and less complicated SARS-CoV-2 testing method could aid in rapid isolation of infected people and prevent new outbreaks. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a promising alternative to current standard methods, though with lower sensitivity.
Researchers found that patients with less pulmonary consolidation on chest CT were more likely to have negative initial lab results for COVID-19 pneumonia. The study used chest CT scans and RT-PCR tests on 21 patients with confirmed COVID-19 pneumonia.
Researchers investigated COVID-19 patients with recurrent positive reverse transcriptase-polymerase chain reaction (RT-PCR) results after hospital discharge. The study found these patients experienced prolonged viral persistence, indicating a need for extended monitoring and potential long-term treatment strategies.
Researchers at Johns Hopkins Medicine have found that the RT-PCR test can produce false negatives in COVID-19 diagnosis, particularly in early stages of infection. The study suggests that the predictive value of these tests may be less accurate than previously thought.
A new algorithm aims to protect operating room team members from Coronavirus Disease 2019 (COVID-19) and conserve personal protective equipment. The guideline assumes patients could be infected unless proven otherwise by a negative RT-PCR test.
Researchers found COVID-19 positive results in sputum and feces samples of patients with negative pharyngeal swabs, suggesting additional body site sampling may be needed. The study highlights the need for further investigation into patient safety and infectiousness after discharge.
A recent study found that chest CT imaging has a sensitivity of 98% for detecting COVID-19, compared to RT-PCR's 71%, making it a reliable and practical method for early diagnosis. This is particularly important given the highly contagious nature of the virus.
Researchers at Nagoya University have created a label-free method for detecting real-time DNA amplification based on refractive index changes in diffracted light. This technique is highly sensitive and can quantify DNA concentrations from 1 fM to 1 pM, outperforming existing fluorescence-based detection systems.
Researchers created a low-cost, portable genetic test for the Zika virus that can detect genetic material and requires no electricity or technical expertise. The test takes about 40 minutes to run and has shown sensitivity equivalent to RT-PCR tests.
A new point-of-care EVD test has been shown to be faster and as sensitive as a conventional laboratory-based method, detecting the virus in just a small drop of blood. The rapid diagnostic test could help identify case contacts and ultimately curb Ebola's spread by quickly confirming or ruling out cases.
A new multiple-target, real-time reverse transcription-PCR (real-time RT-PCR) TaqMan assay can differentiate between patients with latent TB and those with active disease. The assay targets eight human immune markers and has a sensitivity of up to 100% for active disease.
Researchers have developed a novel technique for detecting ALK rearrangements in non-small cell lung cancers (NSCLCs) using quantitative (q)RT-PCR. The assay overcomes limitations of existing methods, including cost and interpretation challenges, with high sensitivity and ease of use.
Researchers have discovered a rapid and reliable test using next-generation real-time PCR systems to detect Shiga toxin-producing E. coli in ground beef. This new method is expected to increase food safety by providing faster, easier, and more reproducible results.
A study published in The Lancet Oncology found that some CML patients can survive up to 2 years without relapse after stopping imatinib treatment, suggesting a potential cure with tyrosine-kinase inhibitors. The study suggests that indefinite treatment may not be necessary and that imatinib can be safely discontinued in some cases.
A new laboratory technique using real-time polymerase chain reaction (PCR) allows for rapid detection of eye pathogens, including bacteria and fungi, in corneal scrapings. This method provides faster diagnosis and treatment for corneal ulcers compared to traditional bacterial culture, which can take up to 48 hours.
Researchers can access optimized methods for RNA isolation, qRT-PCR, and coimmunoprecipitation of RNA-protein complexes from zebrafish and C. elegans. These protocols enable the study of RNA interactions with proteins to drive cellular activities.
New research found that many published clinical genomic signatures have high specificity but very low sensitivity, making them inadequate for most clinical applications. The study suggests that a rigorous approach to evaluating signature quality is necessary to improve the accuracy of sequence-based diagnostics.
A study compared ligase detection reaction (LDR) and real-time PCR methods for detecting low abundant YMDD mutations in hepatitis B patients. LDR was found to be more sensitive than real-time PCR, but the latter was faster and less expensive.
Researchers have developed a new technique to detect and identify wound bacteria, significantly reducing the time it takes for lab personnel to figure out what type of bacteria is present. This allows for more accurate treatment to begin sooner, which can help prevent antibiotic resistance.