Articles tagged with Calcium Imaging
Researchers at Kyushu University develop a new tissue-clearing reagent, SeeDB-Live, enabling repeated, reversible, and real-time imaging of living brains at greater depth and clarity. This breakthrough allows scientists to visualize neural activity in living mice and brain slices, offering new insights into brain dynamics and function.
Researchers at Brown University have created a bioluminescence tool that enables the measurement of activity in living brain cells without damaging them. The CaBLAM tool uses bioluminescent light production to capture single-cell and subcellular activity at high speeds, allowing for longer recordings and reducing hardware requirements.
A recent study published in Nature Communications reveals that the mechanical properties of the developing brain play a significant role in synapse formation and electrical signal emergence. The researchers found that softer regions exhibit higher synapse densities, while stiffer regions show lower densities.
Researchers at Saitama University have revealed that DmMSL10, an ion channel, acts as the Venus flytrap's primary touch sensor. This discovery showcases how plants can sensitively detect gentle stimuli, leading to efficient prey capture and trap closure.
A study found that memories acquired while awake are stored in a more permanent form during REM sleep, requiring the reactivation of only three adult-born neurons involved in memory formation. This process is synchronized with theta rhythm activity and essential for proper memory function.
Researchers at Kobe University have discovered a specific set of neurons that direct mice's attention to peers in need and those who are friends. These 'PV interneurons' play a crucial role in modulating social behavior, including empathy and preference for social targets.
Cryo-optical microscopy captures high-resolution, quantitatively accurate snapshots of dynamic cellular processes at precisely selected timepoints. This technique enables the observation of transient biological events with unprecedented temporal accuracy.
Researchers discovered a two-step mechanism where inhibitory neurons release nitric oxide to rapidly dilate blood vessels, followed by slower, localized vasodilation via astrocyte activation. This breakthrough sheds light on how neural signals are translated into blood volume changes in brain imaging.
Researchers developed a simplified model to explain visual processing in the primary visual cortex, achieving 75% accuracy with fewer layers. The 'minimodels' for individual neurons are just as powerful as large models, providing an accurate and interpretable way to study visual computation.
Researchers have developed CaliAli, an advanced analytical framework that aligns calcium imaging data across multiple sessions, allowing for the continuous tracking of individual neurons. This breakthrough enables long-term brain activity studies and advances understanding of memory formation, retention, and neurological diseases.
Researchers have identified the medial prefrontal cortex (mPFC) as the basis of emotional inference in animals and humans. In a study published in Nature, Xiaowei Gu and Joshua Johansen found that rats can learn inferred emotions by associating a neutral stimulus with an unpleasant experience.
A study by Dr. Keum Sehoon's team identified key neural ensembles in the anterior cingulate cortex that encode empathic freezing, a behavioral response to witnessing distress in others. The researchers found that observing another's pain triggers activation in the ACC as if the observer were experiencing pain themselves.
Researchers have identified three cell types in the median raphe nucleus that control decisions on perseverance, exploration, and disengagement. These findings may help understand neuropsychiatric conditions such as OCD, autism, and major depressive disorder.
The study reveals a unique, ring-shaped organization of the antennal lobe, with specific glomerular clusters encoding different odors. This coding mechanism differs from other insects and vertebrates, with the representation of odor valence encoded in higher brain centers.
Researchers at Washington State University discovered that cannabis activates a set of cells in the hypothalamus, promoting appetite in mice. The study used calcium imaging technology to determine how brain cells responded to vaporized cannabis sativa.
Researchers investigated the role of estrogen receptor beta-positive neurons in the medial amygdala, a region involved in social information processing. They found that MeA-ERβ+ neurons exhibit different roles for receptivity-based and sex-based preferences.
Researchers developed an algorithm to extract bouton-like structures from calcium imaging data, identifying synchronized synapses during fictive locomotion. PQ-clustering outperformed other algorithms in mimicking synaptic activity patterns.
Conscious perception of sound generates specific neuronal assemblies in the brain, differing from spontaneous brain activity. Under anesthesia, similar assemblies are present but lack sound-specificity, highlighting the importance of cortical creativity in sensory processing.
Neuroscientists at Sainsbury Wellcome Centre discovered that individual neurons in the visual cortex of mice are modulated separately by attention and running. The study found that spatial attention and running influence neurons independently, with different dynamics.
Researchers at Osaka University discovered that mutant variants of the RyR1 calcium channel protein are more sensitive to heat than normal proteins, leading to a cycle of activation that can cause malignant hyperthermia. This finding provides new insight into the condition and could lead to preventive and treatment strategies.
Researchers have developed an imaging technique to capture information about brain tissue at the subcellular level, combining seven methods to visualize neural networks and individual cells. This approach allows for a complete picture of brain structure and function, overcoming challenges of imaging tissues at different scales.
The Mini2P allows for live imaging of thousands of neurons, recording complex behavior and cognitive functions in a naturally behaving animal. By mapping neural landscapes across the cortex, researchers can gain insights into high-resolution brain activity and function.
Researchers used holographic stimulation and calcium imaging to study the effects of acute pain on neuronal network activity. They found that spontaneous activity and synchronization between neurons increased during pain, and that blocking N-type calcium ion channels helped restore pain thresholds.
Researchers have developed a new technique called Cal-Light to visualize and control neuronal activity. This tool allows for the observation of specific populations of cells implicated in particular behaviors, enabling precise manipulation and dissection of complex neural circuits.