Articles tagged with Flow Cytometry
Researchers developed new chemical probes to track individual enzymes, enabling direct measurement of protein activity and correcting prior limitations. This allows for a clearer picture of molecular logic in cells undergoing programmed cell death, potentially informing drug discovery.
Scientists have developed a groundbreaking method that can identify and sort bacteria based on their natural fluorescence, allowing for the study of unculturable microbes. This breakthrough enables researchers to investigate how different bacterial shapes contribute to behavior such as disease and growth.
A research team from Nara Institute of Science and Technology developed a dynamic microfluidic channel that adjusts to particle size, increasing impedance flow cytometry's sensitivity and accuracy. The platform also leverages clogging as a strategy to optimize performance.
Researchers have developed a groundbreaking method to observe lysosomes in live suspended cells—quantitatively, in 3D, and without the use of chemical labels. The technology uses holographic tomography in flow cytometry configuration (HTFC) to identify morphological and spatial lysosomal changes in models of lysosomal storage diseases.
A USC team has developed an advanced platform to analyze chimeric antigen receptor (CAR) T cells, revealing how their manufacturing conditions impact effectiveness. The tool uses laser technology to analyze 36 characteristics of a single cell, providing a clearer view of CAR T cell behavior.
A new method has been developed to link individual microbes to their genetic code, providing insights into the activity of microorganisms in coastal sediments. The study reveals a diverse microbial community thriving in environments subject to frequent disruptions from rapid temperature changes and tides.
The Rice team created a low-cost, pump-free flow cytometer that uses gravity-driven slug flow to analyze cells with similar accuracy as conventional devices. The device is powered by AI and can count specific immune cells from unpurified blood samples within minutes.
ChromaTwist has secured a £0.5M Innovate UK Smart grant to enhance its novel dyes for improved bio-imaging. The funding will boost the company's technical development and scale-up for commercial launch.
Researchers have developed EV Fingerprinting, an analytical tool that can characterize extracellular vesicles with minimal sample preparation. This technique may lead to the use of liquid biopsies as a substitute for traditional biopsies for certain patients or diseases.
Researchers have developed a model to enrich sub-populations of cancer cells with high basal levels of mitophagy, promoting CSC features such as self-renewal, proliferation, and drug-resistance. This study highlights the importance of BNIP3/BNIP3L in maintaining cancer stem cell properties.
Researchers developed Epitope Binning-seq to analyze epitopes in monoclonal antibodies. The method accurately classified antibodies into distinct epitope bins, providing valuable insights into their binding patterns and streamlining early antibody drug development.
Researchers have developed a new technique called molecular pixelation, which allows for the analysis of hundreds of proteins simultaneously in individual cells. This provides a more detailed picture of protein distribution and interactions, crucial for understanding cellular function and signaling.
Researchers developed a new method to link genetics and function of individual microbes living without oxygen deep below Earth's surface. The approach enabled discovery of the most active organism in a Death Valley groundwater aquifer, expanding its application to low biomass environments.
Researchers have created a new analytical method to identify and measure small microplastics in the environment. The technique combines flow cytometry with pyrolysis gas chromatography mass spectrometry to characterize and count these tiny particles, providing a more complete picture of their abundance and type.
Researchers developed a noninvasive diagnostic test to identify intact sperm in infertile men with nonobstructive azoospermia. The test uses protein biomarkers AKAP4 and ASPX to visualize well-developed sperm, potentially increasing success rates for surgical sperm extraction. This breakthrough may lead to new male birth control drugs ...
New research from Bigelow Laboratory for Ocean Sciences reveals that coccolithophores can survive in low-light conditions by taking up dissolved organic forms of carbon. This finding challenges current understanding of the biological and alkalinity pumps driving carbon transport in the ocean.
Researchers at São Paulo State University developed a new technique for analyzing male fertility potential using flow cytometry, which can detect and analyze chemical and physical characteristics of human sperm simultaneously. This technique has the potential to enhance the accuracy of fertility prediction and treatment.
Scientists have developed a new method to deliver genetic information to stem cells using nanoparticles coated with a specific polymer, enabling more efficient control over cellular differentiation. This innovation has the potential to improve the efficiency and effectiveness of regenerative medicine treatments.
Researchers developed a calculator to identify patients with multiple myeloma and primary systemic amyloidosis who have a more benign profile, allowing for personalized treatment. The tool predicts survival based on clinical-biological characteristics and has been validated in international series.
Researchers have developed a novel autonomous, submersible, 3D holographic microscope and imaging system to study marine particles and plankton in their natural environment. The AUTOHOLO system achieved 90% accuracy in detecting red tide blooms at varying concentrations, enabling near real-time monitoring and tracking of bloom phases.
A new platform has been established to improve the analysis of dynamic metabolic features in cells. The Raman-based flow cytometry tool allows for the profiling of cell populations without labeling or destroying them, providing a non-invasive big-data type for culture-independent phenomes.
A new class of SARS-CoV-2 antibodies has been shown to neutralise multiple variants of the virus, providing hope for a better antiviral medication. The antibodies work by attaching to a partially hidden part of the virus' spike protein that would be difficult for it to mutate.
Researchers applied scRNA-seq to study hypertrophic cardiomyopathy, identifying novel regulatory interactions and genes driving disease-related swelling. This knowledge can be used to develop new drugs that target underlying causes, reducing disease progression.
A new low-cost, portable instrument uses photonic technology to detect SARS-CoV-2 in saliva samples with high sensitivity and speed. The device can measure small quantities of virus like PCR tests but is as fast as rapid antigen tests.
A novel immune-profiling method can return detailed immune cell type proportions using only DNA from blood, potentially allowing for individualized prediction of outcomes in immunotherapy patients. This approach offers the opportunity to ask and answer questions about the immune system in health and disease.
The new BD CellView Image Technology enables high-speed sorting of individual cells based on detailed microscopic analysis, accelerating discovery research in immunology, cell biology, and genomics. This technology has the potential to unlock new cell-based therapeutic discoveries and transform various fields of biomedical research.
Researchers used next-generation DNA sequencing to detect residual disease in patients treated with CAR-T therapy for acute lymphoblastic leukemia. The study found that DNA sequencing was more sensitive and accurate than flow cytometry in predicting relapse, enabling earlier intervention.
Scientists from Nara Institute of Science and Technology have created a simple and fast method for detecting cell shape as they pass through a microfluidic channel. The team used changes in electrical impedance to measure the asymmetry of individual cells, which may greatly accelerate biological experiments.
Scientists from Bigelow Laboratory discovered microorganisms in crustal rock beneath the Atlantic Ocean, using a new method to study them. The findings show that these microbes survive mostly off carbon from seawater, with some possibly using carbon monoxide for energy.
The study investigates the cellular uptake of Pr3+:LaF3 nanoparticles and their potential as cell nano-sensors. The results show that both nanoplates and nanospheres are easily internalized by A-549 cells via macropinocytosis, leading to an increase in optical inhomogeneity and potentially enabling temperature sensing.
A study published in PLOS ONE introduces a new flow cytometry method for diagnosing male urethritis, which is faster and more precise than existing methods. The study found that gonorrhoea causes the highest inflammatory reaction and bacterial count in urine, highlighting the potential of this technique for rapid screening.
Researchers identified distinct immune profiles, 'Immunotypes,' in 125 hospitalized COVID-19 patients. These signatures correlated with disease severity and provided insights into the human immune response.
A study published in eLife found that doublet immune cells are more common than previously thought and play a crucial role in disease progression. The research reveals that these cell complexes can serve as biomarkers for immune perturbations, potentially allowing for early detection of diseases like dengue fever.
A new method using flow cytometry has been developed to measure the immune response in islet transplant recipients. This standardized approach helps predict patient outcomes and could enable early intervention to prevent rejection.
This special issue showcases recent advances in high-throughput flow cytometry for drug discovery, including novel applications and expert insights. Examples include HTFC for single-cell analysis, biologic drugs, and CAR-T therapy.
Researchers from the Genomics Institute of the Novartis Research Foundation developed a fully automated screening system that solves the slow and low-throughput problem of high-throughput screening in flow cytometry. The system achieves a throughput of 50,000 wells per day, enabling robust phenotypic drug discovery.
Researchers developed a computer machine-learning model that accurately predicts which AML patients will go into remission following treatment. The model was trained using bone marrow data and medical histories of AML patients, achieving 100% accurate predictions for remission and 90% accurate predictions for relapse.
A new diagnostic test has been identified as the most effective method for measuring treatment response in young patients with acute myeloid leukemia, which could lead to a higher cure rate. The test uses flow cytometry to identify minimal residual disease in patient bone marrow, allowing for more intensive therapy to be guided.
Researchers at Penn State have developed a biochip-based device that can rapidly screen cells for leukemia or HIV. The device uses microfluidic drifting technology to focus particles or cells in a single stream, eliminating the need for bulky lenses and mirrors, and potentially reducing costs to $1,000 from current prices of $100,000.
A study has found that high-throughput sequencing can detect minimal residual disease in nearly double the number of leukemia patients as current gold standard method. This technology offers a greater chance of survival by predicting disease relapse sooner.
A new imaging technology uses specially designed dye-containing nanoparticles to simultaneously monitor changes in two intracellular proteins in cancer cells. This could provide a better picture of what's happening in individual tumor cells and potentially improve diagnosis and treatment.
The RHIC will continue to drive standardization in immunology, allowing for better comparison of data worldwide. The center's expertise will help advance three cutting-edge technologies to maturity, including arrayed image reflectometry and quantum dot nanostructures.
The Guava EasyCD4 and EasyCD8 assays provide comparable accuracy to flow cytometry methods but are simpler and more affordable, enabling global access to affordable HIV/AIDS patient monitoring. These lower-cost alternatives can significantly improve quality of care and life for millions of HIV+ patients in resource-limited countries.