Researchers developed a new approach called Co-opting Regulation Bypass Repair (CRBR) that can correct genetic diseases caused by single gene mutations. The method uses the CRISPR/Cas9 system and non-homologous end joining to insert a functional copy of the gene, enabling treatment in all adult tissues.
The Tomographer algorithm transforms gene-sequencing data into spatially resolved images, mapping gene expression patterns across tissues. By analyzing tissue strips and mRNA measurements, the algorithm reconstructs spatial gene-expression patterns in tissues like the brain of the Australian Bearded Dragon.
Researchers from Skoltech identified the role of distant RNA regions in regulating gene expression, revealing their impact on splicing and gene regulation.
Researchers from Kobe University and Chiba University developed a flexible method to create genetic switches for yeast, allowing for the control of gene expression. They successfully produced yeast that biosynthesized orange pigment under AND-gated control, paving the way for sophisticated, artificially regulated cells.
A recent study published in Cell Reports reveals that DNA supercoiling is involved in regulating gene expression, rather than being just collateral damage. The researchers found that specific genes are massively activated in response to stimuli, and topoisomerase TOP2A plays a crucial role in this process.
Researchers from the University of Tsukuba have discovered that interactions between proteins and nitrate control gene expression in legumes, leading to nodulation. The team found that specific proteins with varying DNA-binding properties regulate symbiotic genes involved in nodulation, which is induced by nitrate presence.
Researchers developed a reversible gene editing technology called CRISPRoff that allows controlling gene expression while leaving the underlying DNA sequence unchanged. The new method can silence the vast majority of genes with great homogeneity and in a reversible manner.
Xue Sherry Gao, a bioengineer at Rice University, has won an NIH grant to develop tools that can correct multiple single mutations causing cystic fibrosis. Her approach uses CRISPR/Cas9-based gene editing to fix the faulty CFTR gene, which is difficult to treat with traditional gene therapy.
A peptide nucleic acid-based gene editing technique has been identified as a crucial part of the gene editing toolkit, offering an alternative to CRISPR-Cas systems. The technique, developed by Carnegie Mellon's Danith Ly and Yale University's Peter Glazer, allows for in vivo gene editing using nanoparticles.
Researchers investigated how certain strains of herpes simplex virus (HSV-1) activate gene expression in neurons, finding that different genetic variants induce distinct patterns of gene expression. These findings may help understand why people experience varying levels of symptom severity and frequency of outbreaks.
A recent study by McGill University researchers has mapped gene signatures to functional processes across the human brain, revealing key insights into cognitive and affective processes. The findings suggest that specific genetic signals are linked to attention and memory, while others are associated with emotions and fear.
Researchers found that mice lacking Rev-erb gene exhibit characteristics similar to dawn phenomenon in type 2 diabetes. The study suggests that altered daily rhythm of Rev-erb gene expression may underlie the condition. Further investigations may lead to therapies.
Researchers found that certain genes in inflammatory cells called glial cells increased their activity and grew longer appendages after death. This discovery challenges current understanding of post-mortem gene expression and cell activity, which may impact research on disorders like autism, schizophrenia, and Alzheimer's disease.
A recent study published in Toxicological Research & Application compared the effects of vape aerosol and cigarette smoke on human lung tissue. The research found that exposure to vape aerosol had a minimal impact on gene expression compared to combustible cigarettes.
A new study reveals that SARS-CoV-2 infects cells with high expression of ACE2 receptor and protease, found in lung epithelium and other organs. The study suggests that elderly people, men, and smokers are more severely affected due to increased viral entry factor expression.
A study published in Genome Biology found that small changes in RNA due to RNA editing can have large effects on gene expression and phenotype. The researchers identified 3,117 unique RNA editing events associated with genetic variation, including those linked to complex traits or diseases.
Scientists have identified a region within the GLI1 gene responsible for regulating its expression, providing a potential target for cancer treatment. Removing this region eliminates GLI1 activity, halting excessive cell division characteristic of cancer.
Researchers have developed an immune tolerance platform called ImmTORTM to overcome challenges in gene therapy, including immunogenicity and durability. The addition of ImmTOR nanoparticles to AAV vectors has shown potential to enhance efficacy, safety, and durability by mediating more efficient transgene expression.
Researchers have developed a new method, Cre-Controlled CRISPR, which combines the benefits of the Cre/lox system and CRISPR/Cas9 genetic scissors for conditional gene inactivation. This approach allows for faster and easier gene editing with reduced labor needed to flank genes with lox sequences.
Researchers found that Polycomb repressive complex 1 (PRC1) and Polycomb repressive complex 2 (PRC2) maintain the skin-specific gene expression pattern necessary for proper development of the skin. The study suggests that targeting both complexes may be a more effective form of treatment for certain cancers.
The study found that AKT isoforms have distinct expression patterns in triple-negative breast cancers, with AKT1 associated with invasiveness and sensitivity to drug treatment. Loss of AKT1 function was linked to reduced sensitivity to cisplatin, while the presence of AKT2 promoted stemness and invasion.
Researchers analyzed mRNA expression levels of molecular markers ERCC1 and thymidylate synthase in patients with resected stage II-III non-small cell lung cancer. The study found no correlation between these markers and overall survival, contradicting previous hopes for a personalized chemotherapy approach.
Researchers developed a gene therapy strategy to treat Leber congenital amaurosis by adding copies of the normal CRX gene under its native control mechanism. This approach restored some CRX protein function and drove expression of opsins in patient-derived retinal organoids.
Researchers are using cutting-edge MRI sensors to map brain responses to drugs of abuse and identify genes that promote neuron survival. They plan to apply this technology to study Parkinson's disease, hoping to uncover new therapeutic targets.
This atlas of S. pneumoniae and host gene expression provides a comprehensive understanding of the pathogen's adaptation to different body locations and the host's response. Certain bacterial genes are highly expressed across all anatomical sites, making them ideal targets for new treatments.
Researchers at Tokyo Institute of Technology create kinetic model to study site-specific histone modifications and their impact on chromatin accessibility. The model shows that acetylation increases chromatin accessibility threefold, highlighting the potential for accurate quantification of epigenetic effects.
Researchers propose minimal extension to classic equilibrium model, capturing high specificity and noise in eukaryotic gene expression. The new model suggests a trade-off between specificity and noise, with high specificity leading to increased noise.
A study reveals that methylation of the DVDMR region in the IGF2 gene determines its protein expression levels in breast cancer cells and tissues, suggesting a potential novel epigenetic biomarker for BC aggressiveness. The findings imply that IGF2 levels contribute to BC metastasis and chemoresistance.
The NIH Platform Vector Gene Therapy (PaVe-GT) project uses AAV9 as a platform vector to develop gene therapy products for four rare diseases. The project aims to improve the delivery of therapeutic genes into target cells, paving the way for access to gene therapy for patients with difficult-to-treat conditions.
Researchers found that dietary intervention increased skin gene expression manifold, with raw food activating the immune defense system and increasing antioxidant production. This study suggests that a raw diet may help activate the immune defense of dogs, potentially reducing the risk of atopic dermatitis.
Researchers at UW Medicine created two cell atlases that map gene expression and chromatin accessibility in human development, providing unprecedented data for understanding cell differentiation. The atlases identify 77 main cell types and approximately 650 cell subtypes, shedding light on the regulatory 'grammar' of the cell.
Researchers have developed a new approach to prevent toxicity seen in sensory neurons of dorsal root ganglia after gene therapy to treat neurological disorders. The approach involves modifying a transgene with a microRNA target, which reduces transgene expression and eliminates toxicity.
Heidelberg researchers discovered that RNA synthesis and protein translation play crucial roles in shaping organ functions. The study analyzed over 100 billion gene expression fragments, revealing the importance of regulatory mechanisms at both layers of gene expression.
Researchers found that inducing liver regeneration with thyroid hormone boosted CRISPR/Cas9-mediated gene correction, achieving 10.8% and 3.5% efficiency rates in neonatal and adult mice respectively. This discovery could lead to more efficient gene therapy for human diseases.
Researchers found that methylation of a specific region in the IGF2 gene, known as DVDMR, determines IGF2 protein expression levels in breast cancer. High IGF2 levels are associated with increased BC aggressiveness and metastasis.
A study found that vitamin D status during pregnancy is associated with altered gene expression in the placenta, which may contribute to the development of preeclampsia. The researchers also observed a correlation between sFLT1 and VEGF gene expression levels.
A Yale study shows that epigenetic mechanisms play a crucial role in shaping the evolution of gene networks in yeast. The research suggests that epigenetic factors can be passed on to offspring, contributing to stable and heritable gene expression states.
A new gene expression study by McLean Hospital researchers identifies CREB as a molecular switch regulating fear and extinction. Targeting this gene in neurons may provide new insights and treatments for anxiety and post-traumatic stress disorder.
Research found that vitamin D3 reduces both HAS2 gene expression and hyaluronic acid synthesis in multiple models of breast cancer. The study suggests a novel role for vitamin D3 and the VDR in control of HA synthesis, which likely contributes to its anti-cancer actions.
University of Texas at Dallas researchers have identified the source of fluctuations in gene expression between identical cells, finding that transcription is the primary driver of cellular noise. This discovery has significant implications for developing targeted therapies and gene therapies.
Researchers at Tokyo Medical and Dental University found that laser stimulation inhibits sclerostin expression in bone without causing inflammation, providing a new treatment possibility for osteoporosis. This method may aid the development of laser-based therapies to promote bone regeneration.
Research highlights sex differences in gene expression, finding over a third of genes display sex-biased expression in at least one tissue. Sex-linked disparities contribute to increased cancer rates in males and autoimmune disorders in females.
The GTEx project reveals population-specific and sex-specific differences in gene expression that can inform how gene variants impact aging and disease. The findings highlight the importance of accounting for sex differences and individual variation in future studies.
Researchers found sex differences in gene expression in almost every type of human tissue, with effects observed in genes related to disease and clinical phenotypes. Sex biases were discovered in genes relevant to drug metabolism and placental development.
The GTEx project has mapped genetic variations that affect gene expression across over four dozen tissues, revealing the importance of cell type in understanding gene regulation. The study cataloged QTLs governing the expression of over 23,000 genes, highlighting the complexity underlying genetic control of gene expression.
A new study from Northwestern Medicine reveals that biological sex has a small but significant impact on gene expression in every type of human tissue. The researchers found over 37% of genes were expressed differently in males and females, with diverse molecular functions involved, including disease-related traits.
Researchers analyzed 1,903 RNA-seq datasets from 182 projects to reveal a complex history of gene family trees, allowing them to study the evolutionary dynamics of gene expression patterns. Gene duplication plays a key role in expression pattern shifts, and preadaptive propensities exist for genes to be utilized in other organs.
A new study identifies a specific gene's role in fertility, finding mutations in this gene are associated with early menopause. The researchers used genetic techniques to find genes involved with eye development and discovered the gene is essential for reproductive organs.
A new investigational therapy has shown to prevent the onset of Dravet syndrome symptoms in a mouse model, increasing gene expression up to 100 percent in neurons. The treatment uses an antisense oligonucleotide (ASO) that is reversible and non-invasive.
Scientists from the University of Tsukuba found that retrotransposons may change how genes are expressed in melons. In a study published in Communications Biology, researchers discovered that retrotransposons had a role in altering gene expression when melon genomes were diversifying and affecting fruit ripening.
A new study identifies two genes, TMEM106B and RBFOX1, that regulate gene expression in the aging brain and are linked to both Alzheimer's disease and Limbic-predominant Age-related TDP-43 Encephalopathy (LATE). The findings suggest a shared molecular basis for these diseases, which may lead to new treatments and prevention strategies.
A breakthrough study using gene therapy to target the inner retina has prevented blindness in a mouse model of CLN3 Batten disease. The treatment led to significant survival of bipolar cells and preserved retinal function, according to researchers.
A consensus statement evaluates the use of prognostic genetic expression profile testing in melanoma, cautioning against routine use due to limitations. The statement aims to assist clinicians in determining when and how gene expression profiling should be adopted in clinical practice.
Researchers used the MADM technique to investigate how cells respond to changes in genomic imprinting. They found that cells activate certain gene groups involved in cell death, growth, and synapse development, particularly in astrocytes.
A recent study has found that elderly Alzheimer's patients are more susceptible to COVID-19 infection due to increased expression of the ACE2 gene, which acts as an entry receptor for SARS-CoV-2. The research, published in the Journal of Infection, suggests that elevated ACE2 levels can lead to a higher risk of infection.
Scientists have discovered that the Anaplasmosis bacterium interferes with tick gene expression to survive and spread to new hosts. The study found that the bacterium reduces a regulatory molecule's production, leading to increased levels of Organic Anion Transporting Polypeptide (OATP), which enables its spread to vertebrate hosts.
Researchers analyzed bacterial RNA and protein expression patterns during plant-bacterial interactions, revealing the impact of plant immunity on bacterial mRNAs and proteins. The study identified previously unknown transcriptional regulators controlling bacterial gene expression and virulence.
A study found that survivin positivity by IHC correlates with shorter survival in Neuroendocrine tumors. Radiation exposure increases BIRC5 gene expression, which is associated with an inferior survival. Survivin long peptide-mimic vaccine shows promise in preclinical studies.
A research group from Kumamoto University found the transcription factor RUNX3 plays a cancer growth function in what was previously thought to be a tumor suppressor. RUNX3 is also linked to the initiation and propagation of MDS stem cells, making it a promising new therapeutic target.
Researchers found that ovarian cancer patients with a modified BRCA1 gene do not respond better to platinum chemotherapy or have a better prognosis than those with the normal functioning gene. However, they did live longer on these treatments compared to those without any gene modifications.