A new RNA-based technology called StitchR facilitates effective use of gene therapy for difficult-to-treat, large-gene diseases like muscular dystrophies. It delivers two halves of a gene separately, resulting in seamless reconstitution of large mRNA in affected tissues.
Researchers have found that combining the VIGex gene expression signature with liquid biopsy analysis improves response prediction to immunotherapy in patients with advanced solid tumors. The study analyzed tumor samples and circulating tumor DNA (ctDNA) by liquid biopsy, showing that the VIGex-Hot subgroup was associated with higher o...
The new textbook, Gene Regulation and Epigenetics: How Science Works, provides an in-depth understanding of gene regulation and epigenomics, essential for graduate students across biomedical fields. The book highlights the role of transcription factors, chromatin dynamics, and non-coding RNA in shaping gene expression.
Researchers have developed a Virus-Induced Gene Silencing (VIGS) system in taro, allowing for rapid verification of gene functions and enabling breeding of high-quality taro varieties. The system has shown promise in reducing gene expression and altering starch content in taro corms.
Using state-of-the-art technology, researchers identified specific steps needed for CRISPR to become active and perform its gene editing function. Understanding these mechanisms could lead to improved designs for CRISPR-based gene editing.
Researchers developed a new system to produce high-yield proteins in lettuce by silencing specific genes. This method increases recombinant protein expression by over two times, making it a promising alternative for large-scale production.
ImSpiRE redistributes gene expression data across tissue sections using histological image features, allowing for finer resolution and capturing subspot-level detail. This improves visualization of gene expression with unprecedented clarity, aiding in identifying tissue domains and detecting complex interactions.
Researchers at ChristianaCare Gene Editing Institute use CRISPR tools to safely disable gene mutation linked to treatment-resistant melanoma. The approach targets melanoma tumor cells while leaving healthy cells alone, restoring sensitivity to anticancer drugs.
New research from Sharon Torigoe at Lewis & Clark College confirms the importance of low-affinity binding sites for Klf4 gene enhancers in naive-state pluripotent stem cells. This discovery advances scientists' knowledge of gene expression mechanisms and has implications for regenerative medicine and understanding human disease.
Scientists from Spirovant Sciences describe a novel adeno-associated virus (AAV) gene therapy called SP-101 that has been optimized for efficient human airway cell transduction. After single dose inhaled delivery, the vector showed consistent expression of a functional and regulated shortened human CFTR minigene.
Researchers led by Kathrin Schrick discovered a new type of molecular interaction between a protein and a phospholipid molecule, which regulates gene expression levels. This finding has significant implications for developing crops that can efficiently use phosphorus, essential for plant growth, to withstand drought and climate change.
Researchers identified a long-postulated hidden spatial grammar embedded in DNA, which holds the key to understanding how gene activity is encoded. The study found that transcription factors have a complex function, acting both as activators and repressors, and their position relative to genes influences gene expression.
Researchers found that HIV-infected individuals with impaired immune recovery (INRs) have significantly higher expression of IFI27 and IFI6 in their peripheral blood compared to those who recovered well (IRs). A predictive model using these genes, along with age and CD4+ T/CD8+ T ratio, accurately identified INRs. These findings sugges...
The Rice University lab has developed a new noninvasive technique, called REMIS, that can measure gene expression and gene therapy delivery in specific brain regions using ultrasound. This technology could revolutionize brain-based gene therapy for neurodegenerative diseases.
A new open-source platform, Nova-ST, is transforming gene expression profiling in tissue samples by offering affordable and high-resolution spatial analysis. This approach allows scientists to map gene expression across a tissue section with a spatial context, enabling the study of complex biological processes.
This study investigates transposable elements in 14 Rosaceae genomes, revealing distinct evolutionary patterns and effects on genome size and gene expression. Gene near recent TE insertions are associated with adversity resistance, while those near older insertions link to morphogenesis, enzyme activity, and metabolic processes.
A new DNA methylation array can assess methylation levels of genes in imprint control regions, which regulate the expression of imprinted genes. The array has 22,000 probes and can identify potential links between environmental exposures and epigenetic dysregulation in diseases.
Scientists at deCODE Genetics found that genome variants drive the correlation between DNA methylation and gene expression. The research uses new nanopore sequencing technology to analyze DNA sequences in real-time, revealing a link between noncoding sequence variants and diseases.
Research on Lotus japonicus reveals periodic gene expression with a six-hour rhythm, influencing rhizobial infection and nodule distribution. Cytokinin maintains this oscillatory gene expression, ensuring proper root nodule formation and infection thread distribution.
A new study has generated a global catalog of human gene expression data from around the world, increasing representation of understudied populations. The increased diversity empowers researchers to attain more-accurate insights into genetic factors driving human variation and disease risk.
A study has revealed the genetic complexities of almonds, highlighting significant heterozygosity and structural variants' influence on gene expression. This research paves the way for targeted breeding strategies to enhance desirable traits such as disease resistance and yield in almond cultivars.
Researchers at Istituto Italiano di Tecnologia and EMBL unveiled how to modulate gene expression using small molecules. The study aims to develop new drugs specific to genetic mutations or alterations responsible for the onset of tumors or genetic diseases.
A recent study found that oleacein increases BDNF expression in human neuronal cells and reduces depressive behavior in mouse models. Gene expression analysis reveals the activation of cell cycle and neurogenesis processes, as well as a decrease in inflammatory response.
This study found that TIMP-1 induces the expression of transcription factor Fli-1, which elevates MCP-1 expression and promotes hepatic macrophage recruitment. siRNA-TIMP-1 alleviated liver fibrosis by reducing macrophage migration and MCP-1 expression.
A CNIC team has developed iSuRe-HadCre, a novel genetic tool that enables precise and efficient gene modification in mouse models. This new technology overcomes limitations of existing methods, offering improved efficiency, precision, and applicability in biomedical research.
Researchers found that expression of Gstt1 enables cancer cells to change their environment, allowing them to grow in new locations. The discovery could lead to new strategies for treating metastatic cancer, particularly pancreatic cancer.
Researchers from the University of Illinois have used CRISPR/Cas9 to alter the upstream regulatory DNA of a food crop, increasing gene expression and improving downstream photosynthesis. This approach, which does not require adding foreign DNA, has shown promising results in increasing photosynthetic activity in rice.
A study found that low doses of filtered kretek cigarette smoke altered rat lung histometric measurements, increasing the size of respiratory bronchioles. P53 gene overexpression was also observed in response to exposure. The findings suggest potential health risks from even low levels of kretek cigarette smoke.
A novel gene cluster expression analysis was introduced to potentially expand treatments for more lung cancer patients. The study found that a gene cluster expression index can classify cancers with dramatically different recurrence risks, with higher risk groups suitable for targeted therapy or immunotherapy.
Researchers found elevated expression of RPGRIP1L in invasive breast cancer specimens, correlating with shorter survival times and unfavorable clinicopathological features. The study identified 50 genes and 15 proteins related to RPGRIP1L expression, including those involved in immune response and metabolism.
The study identifies key genes differentially expressed in individuals with familial hypercholesterolemia (FH) and their association with atherosclerosis. These findings provide insights into the development of cardiovascular diseases and suggest potential therapeutic targets for alleviating their impact.
A study found that dopamine inhibits the expression of HBV surface and e antigens in HepG2 cells, increasing interferon-stimulated gene 15 expression. The JAK/STAT pathway was also activated, leading to decreased HBV DNA and antigen expression in a mouse model.
A new machine learning method called scGHOST has been introduced to identify single-cell 3D genome subcompartments and connect them to gene expression patterns. This can reveal the spatial organization of chromosomes within the nucleus, shedding light on how DNA structure influences gene expression and disease processes.
The study identifies neurons as the primary cell subtype sensitive to iACS, with minimal gene expression changes within neurons. Rgs9 is found to be reduced in expression, while unilateral iACS application diminishes its positive neurons in the ipsilateral hemisphere.
A Kyoto University research group developed RENGE, a computational model to estimate gene regulatory networks in multicellular organisms. The method measures time-series gene expression and uses the proprietary model to infer regulatory dynamics.
Researchers investigated the molecular mechanism of serum iron overload-induced BMP/SMAD pathway and hepcidin expression. SMURF1, a master regulatory function, mediates Holo-Tf-induced SMAD1/5 activation and hepcidin expression. The inhibition of SMURF1 may represent a therapeutic strategy for iron overload-related diseases.
Researchers found similar gene activity changes in brain tissue from people with schizophrenia and older adults, suggesting a shared biological basis for cognitive decline. The study identified the Synaptic Neuron and Astrocyte Program (SNAP), which is tightly coordinated between neurons and astrocytes.
Research reveals that physical inactivity and chronic inflammation drive secondary aging, altering gene expression in skeletal muscle. The study identified approximately 4,000 genes affected by physical inactivity and inflammation, while fewer than 200 were linked to primary aging.
Researchers discovered surprising secrets about how fruit flies adapt their senses to different environments through gene expression patterns. Thousands of genes had undergone significant changes in expression, shaping unique olfactory landscapes of different fly species.
Rice bioengineer Jerzy Szablowski and colleagues have engineered a synthetic serum marker that enables non-invasive neural monitoring by tracking gene expression dynamics in the brain. This breakthrough allows researchers to investigate brain development, cognitive function and neurological diseases more effectively.
Researchers developed a high-resolution cell type deconvolution mode method using CIBERSORTx to analyze pooled skin bulk transcriptome data, identifying eight different skin cell types. This allowed for more accurate analysis of gene expression changes post-burn injury and exploration of new targets for clinical wound healing.
Researchers at Baylor College of Medicine have developed a technology to effectively regulate gene expression, promising a safer approach to gene therapy. The system uses small molecules to interact with RNA, allowing for precise control over protein production and therapeutic window maintenance.
Neuronal activity stimulates gene expression in human brain cells by influencing transcription factors and chromatin modifiers, particularly CREB and CBP. The interaction between CREB and DNA requires prior acetylation mediated by CBP to activate gene expression.
A new genetic atlas details the gene expression programs that drive zebrafish development, revealing insights into human embryonic growth and the origins of diseases such as gastrointestinal disorders. The atlas provides a comprehensive resource for studying cell development and differentiation in vertebrates.
Researchers found that Wnt signaling pathway dysfunction can lead to increased immune infiltration and activation, resulting in a heightened responsiveness to immunotherapy in colorectal cancer patients with mismatch repair deficiency. This study suggests a potential new strategy for treating MSI-positive CRC patients.
The Cre-LoxP system's specificity is compromised due to non-specific promoters driving Cre expression, leading to inaccurate results. This limitation requires careful consideration for proper interpretation of experimental outcomes.
A study by Max Planck researchers has discovered an epigenetic regulator MSL2 that ensures the expression of both alleles of haploinsufficient genes, crucial for human health. This mechanism allows for tissue- and cell-type specificity in gene dosage, opening new directions for understanding diseases and developing potential treatments.
A study by researchers from Osaka University found that HSP47, a collagen-specific molecular chaperone, is significantly expressed in fat tissue and correlates with body fat levels. High or low HSP47 expression was linked to high or low body fat levels in both humans and mice.
The study analyzed 10,000 gene expression signatures to assess their prognostic ability, finding that they lead to accurate patient prognosis in no more than 80% of cases. The researchers emphasize the need for a comprehensive approach incorporating molecular, clinical, histological, and other factors to ensure an accurate prognosis.
Researchers identified abnormal keratin expression patterns in senescent ocular surface cells, which may contribute to severe ocular surface diseases. Gene expression profiles showed substantial differences between senescent and non-senescent cells, highlighting their potential role in pathology.
Researchers discovered a gene called FOXM1 that plays a crucial role in repairing blood vessels and improving survival rates. A low-dose repurposed drug, decitabine, was found to reactivate the gene and promote vascular repair in aged mice. An alternative approach using endothelium-targeted nanoparticle gene delivery also showed promise.
Researchers discovered a pair of genes whose expression by immune cells in tumors is linked to patient outcomes and controls human cancers. The study identified a gene expression ratio that predicts tumor behavior and potential therapy targets, offering new insights into cancer treatment.
A new study shows that estrogen receptor (ER) expression in ductal carcinoma in situ (DCIS) is multi-clonal, meaning it varies across different cells. This finding has significant clinical implications, as it may help avoid overtreatment and undertreatment in DCIS patients.
Scientists at Tokyo Institute of Technology identified ApSigma, a nuclear-encoded apicoplast RNA polymerase σ subunit that coordinates gene expression with the life cycle of Plasmodium falciparum and host circadian rhythm. Melatonin is shown to increase apicoplast transcription and expression of the apSig gene.
A new study by researchers at NYU and the New York Genome Center combines deep learning with CRISPR screens to control human gene expression. The model predicts on- and off-target activity of RNA-targeting CRISPRs, enabling precise gene controls for developing new therapies.
Researchers develop predictive model to control gene expression with high accuracy, enabling precise modulation of gene dosage. The AI-powered tool uses deep learning to predict on- and off-target activity of RNA-targeting CRISPRs, holding promise for treating viral infections and developing new therapies.
Researchers developed a new tool, GASPACHO, to capture dynamic changes in gene expression along the innate immune response. They identified a gene variant affecting COVID-19 susceptibility, shedding light on molecular mechanisms underlying disease pathogenesis and identifying potential therapeutic targets.
A novel gene delivery procedure was successfully applied to baboons, achieving therapeutic levels of human factor IX gene expression lasting up to 200 days. The study demonstrated the efficacy of repeated hydrodynamic gene delivery into the same liver lobes, with no significant adverse events observed.
A new study published in Nature Genetics examines the relationship between gene expression and ancestry, revealing ancestry-specific patterns of genetic architecture. The research analyzed whole genome and RNA sequencing data from African American and Hispanic/Latino children, exploring ancestry-related differences in gene expression.
The LY6 gene family has been found to be overexpressed in uterine corpus endometrial carcinoma (UCEC), leading to poor patient survival. Several LY6 genes have been identified as potential tumor-associated antigens and biomarkers for UCEC detection and prognosis.