Articles tagged with Enzyme Production
Researchers at Nagoya University have developed a new method called SMART that accelerates enzyme evolution and reduces costs by accelerating the selection period from weeks to days. The system uses mRNA display, next-generation sequencing, and bioinformatics to identify superior enzyme variants.
Scientists have identified a previously unknown genetic disease, MINA syndrome, which damages motor neurons and affects movement and muscle control. The disease is caused by a rare genetic mutation in the NAMPT protein, leading to symptoms such as muscle weakness, loss of coordination, and foot deformities.
A study on Aspergillus oryzae found that increased cell volume and nuclear number contribute to its high enzyme production capacity. The fungus's hyphae thicken, resulting in a tenfold increase in cell volume, while the number of nuclei per hyphal cell also rises tenfold.
Researchers propose a new framework describing living matter as a double cascade spanning 18 orders of magnitude in space and time, with critical points marking the emergence of self-replicating machines and complex societies.
Researchers achieved significant improvements in ethanol yields by genetically modifying cyanobacteria to optimize carbon flow and overexpress key enzymes. Modified strains produced ethanol at rates between 0.24 and 3.8 g/L, demonstrating robust performance improvements.
The study reveals the genes that enable plants to make DMSP, allowing them to thrive in salty and drought conditions. This breakthrough could improve agricultural productivity in nitrogen-poor soils, making crops more sustainable in the face of global climate change.
The team created a new method by adding two different enzymes to the existing reaction, increasing conversion rates from 46% in 7 hours to 80% in 5 hours. This approach also improved fumaric acid production efficiency from 10% to 16%.
Researchers at Tokyo University of Science discovered a natural tyrosinase inhibitor from Corynebacterium tuberculostearicum that inhibits melanin synthesis and provides an alternative to toxic hydroquinone-based products. The compound, cyclo(L-Pro-L-Tyr), exhibits low toxicity and potential benefits for hyperpigmentation treatment.
Research from the University of Illinois highlights the potential of organic nanozymes for broader applications beyond traditional uses of inorganic nanozymes. The development of sustainable, environmentally friendly materials offers a promising solution for various industries.
Researchers isolated three bacterial strains that produce amylase enzymes from sugar factory waste, showing great potential for production. The optimal conditions for amylase production were found to be 37°C and pH 7.0, leading to increased enzyme activity.
The fungus secretes enzymes that transform tea chemical components through oxidation, degradation, and condensation reactions. This results in high-quality fermented teas with enhanced flavor and health benefits.
Researchers at Tokyo University of Science have developed an enzyme that converts ferulic acid from plant waste into vanillin, a classic flavor compound. The engineered enzyme exhibits high conversion activity and affinities, making it suitable for large-scale commercial production.
Researchers discovered a crucial amino acid exchange that enables PsiM to carry out double methylation during evolution. The enzyme plays a key role in psilocybin production, with implications for biotechnological production of the active ingredient.
A team of scientists discovered new fusion sites in protein evolution that enable faster and more targeted drug development. By combining evolutionary processes with synthetic biology, they created customized biological drugs with improved therapeutic properties.
Researchers developed a novel solution for Pichia pastoris enzyme production, utilizing cyanobacterial biomass to support efficient and sustainable industrial processes. The study reveals the potential of cyanobacterial biorefineries to generate high-performing enzyme-producing strains.
Scientists at IISc have developed an enzyme mimetic called NanoPtA that can degrade toxic chemicals in industrial wastewater effectively in the presence of sunlight. The nanozyme is highly specific and robust, making it suitable for large-scale industrial use.
A KAUST researcher has created a highly sensitive glucose detector based on a thin-film transistor that can measure glucose levels in saliva with high accuracy. The device uses an enzyme to oxidize glucose present in the saliva, producing electrons that indicate the glucose concentration.
A research team at Ritsumeikan University has identified the elusive ApiT gene in celery, crucial for apiin synthesis. The discovery may pave the way for efficient biosynthesis of apiin, a compound with potential health benefits and medicinal uses.
Brazilian researchers discovered an enzyme that can replace traditional catalysts in the production of aviation biokerosene, increasing renewable biofuel output. The enzyme, OleTP RN, promotes deoxygenation and is selective for different sizes and types of carbon chain.
Researchers investigated hepatic hydrogen sulfide production in a mouse model of Hutchinson-Gilford Progeria Syndrome (HGPS) and found reduced H2S levels in RC-fed mice, with partial rescue on high-fat diet. This study suggests that accelerated aging in HGPS may be partially explained by reduced hepatic H2S levels.
The new homogeneous catalyst enables the direct synthesis of hydrogen peroxide with improved efficiency and safety. The process requires only one step and no separation of gases from the reaction flask.
A new study found that a medication previously used to treat seizures can reprogram donor hearts to boost the production of beneficial enzymes, increasing storage time and improving post-transplant function. This technology could lead to a paradigm shift in extending heart storage and reducing primary graft dysfunction.
Researchers have discovered a way to produce limonoids, a family of valuable chemicals with potential as bee-friendly insecticides and anti-cancer drugs. By identifying the enzymes required for production, they can now use host organisms to create these compounds in a more sustainable way.
Researchers at EMBL Grenoble have discovered that THC inhibits the human enzyme autotaxin, which is involved in cancer, inflammation, and pulmonary fibrosis. This finding provides new molecular insights into the therapeutic effects of medical cannabis.
Researchers at Max-Planck Institute for Terrestrial Microbiology have deciphered the biosynthesis of benzobactins, a class of natural compounds with special biological activity. The study reveals that these compounds are widespread in diverse bacteria and could be excellent candidates for future drug therapy.
Researchers at King Abdullah University of Science & Technology have developed a method to produce crocins, a key ingredient in saffron, using a common garden plant. This breakthrough could lead to sustainable and efficient production of these compounds for pharmaceuticals, food coloring, and flavor additives.
The research team will analyze the remaining two enzymes necessary for riboflavin production and build a 'riboflavinator' in a test tube. This understanding could lead to improved methods for treating diseases and improving public health.
Researchers identified eight new microorganisms that cleave ether bonds in the lignin-based compound-2-phenoxyacetophenone. These discoveries could enhance our understanding of the carbon cycle and facilitate biotechnological applications for lignin commercialization.
Scientists from Nagoya University investigate the formation of air channels in wetland plants, which help them survive floods and droughts. The study reveals that a phytohormone called auxin is required for normal root growth, and two factors lead to the induction of aerenchyma formation in response to flooding.
A new chewing gum infused with plant-grown ACE2 protein can neutralize the SARS-CoV-2 virus in saliva, potentially reducing transmission risk. The gum, developed by researchers at the University of Pennsylvania, was tested on saliva samples from COVID-19 patients and found to significantly reduce viral load.
Scientists have developed a method to produce strigolactones, a group of plant hormones that prevent excessive budding and branching. By combining yeast and bacteria, researchers can synthesize these hormones from microbes, providing a promising alternative to traditional methods.
A recent study published in Cell Metabolism found that reducing naturally occurring errors in protein synthesis improves both health and lifespan. By engineering a mutation in ribosomes, researchers observed fewer protein mistakes and improved heat resistance, leading to longer lifespans in yeast, worms, and fruit flies.
Researchers have identified a promising strategy for combating antibiotic-resistant bacterial infections by targeting the production of hydrogen sulfide. The approach, which inhibits the enzyme cystathionine gamma-lyase, enhances the deadly effects of antimicrobials on drug-resistant pathogens.
Researchers have programmed microalgae to produce fatty acids of different lengths, creating a more flexible cell factory for producing industrially relevant oils. This breakthrough could lead to the development of designer oils with various fatty acid chain lengths.
Researchers at KAIST successfully produced carminic acid from glucose in engineered Escherichia coli, overcoming the need for complex purification processes and protein contaminants. The development of a generally applicable C-glucosyltransferase also enables the production of other valuable natural products.
Research reveals that macrophages, key immune cells, respond to pathogens and stress through circadian-controlled metabolism. The study showed an incredible amount of circadian timing in macrophage behavior, but the clock regulated immunity in unexpected ways.
Researchers at Umea University have found that a substance called hyaluronan is responsible for the jelly-like substance in COVID-19 patients' lungs. The discovery could lead to new effective therapies, including treatments using cortisone and Hymecromone.
Researchers at the Brazilian Center for Research in Energy and Materials (CNPEM) have developed a low-cost platform for producing enzymes that break down biomass into fermentable sugar for biofuel conversion. The enzyme cocktail, produced by genetically engineering a fungus, has significant potential industrial applications.
Research investigates cyclosporin variants for their potential in regulating mitochondrial activity, shedding light on the structure-activity relationship. The study's findings suggest that certain cycloprotein molecules may be useful in treating diseases related to mitochondrial dysfunction.
Minor ginsenosides have diverse pharmacological activities, including anti-cancer, anti-diabetic, neuroprotective, immunomodulator, and anti-inflammatory effects. The utilization of microorganisms and their enzymes for biotransformation and biosynthesis are considered highly specific, safe, and environmentally friendly production methods.
A research team has developed a microbial strain capable of producing succinic acid with the highest production efficiency to date. The strain, isolated from a Korean cow's rumen, was engineered through systems metabolic engineering and enzyme engineering to produce 134 g per liter of succinic acid.
Scientists at DTU Biosustain successfully produced psilocybin de novo in yeast, eliminating the need for expensive substrates and enabling large-scale fermentation. This breakthrough could pave the way for the commercial production of psilocybin as a potential treatment for depression and other psychological conditions.
Researchers have visualized the first structure of a lipin enzyme, crucial for triglyceride production, revealing how it regulates fat storage and its role in diseases like heart disease and obesity. The study provides insights into mutations that lead to abnormal triglyceride production.
A Kobe University research team developed a method to produce high rates of D-lactate using cyanobacteria, which could lead to the creation of biodegradable plastics. The team used genetic engineering and dynamic metabolomics to optimize D-lactate production, achieving a rate of 26.6g/L from CO2 and light.
Researchers at Chiba University successfully recreated the metabolic evolution of plants producing alkaloids by inserting a gene into a model plant. This method identifies newly generated substances, including cadaverine, 5-aminopentanal, and δ-valerolactam, which can be used to produce new compounds for drug industries.
Researchers developed a method to produce methyl anthranilate, a common grape flavor compound, using engineered bacteria. The production process reached levels of 4.47-5.74 grams per liter, a significant amount compared to traditional methods.
Scientists at Kobe University created a metabolic pathway and engineered enzymes in microbes to optimize pharmaceutical raw material production. They used a 'Design, Build, Test, Learn' workflow to systematically optimize production processes, resulting in improved alkaloid yields.
Scientists at Technical University of Denmark have discovered 9 genes that can be silenced to increase protein production in engineered yeast cells by 2.2-fold. This breakthrough method has significant implications for industries producing biopharmaceutical proteins and industrial enzymes.
Researchers at the University of Pennsylvania have developed a new method to produce enzymes in plants, which can be as effective as traditional microbial-derived enzymes. The plant-grown enzymes are cheaper to produce and shelf-stable, making them a game-changer for industries such as textile manufacturing and juice production.
Researchers at MUSC have shown that restoring the enzyme's ability to produce nitric oxide can help protect against heart disease in lupus patients. The study suggests that this approach could also be relevant to heart disease more broadly, by promoting endothelial cell function and reducing inflammation.
Researchers successfully developed yeast to produce large quantities of stevia, a zero-calorie sweetener, cutting out the need for plant extraction. The study aims to improve the production process and create next-generation no-calorie sweeteners with better taste.
A new study introduces CasPER, a CRISPR/Cas9 method that diversifies enzymes without additional engineering. It enables the efficient integration of large DNA fragments, allowing for hundreds of thousands of enzyme variants to be generated.
Researchers at the University of Adelaide found a link between enzymes involved in malt production and a specific tissue layer within barley grains. The study showed that grains with more aleurone had increased enzyme activity, which could lead to improved brewing processes and new malts.
The University of Basel researchers have solved the mystery of how the ACC enzyme assembles into distinct filaments, revealing its impact on enzymatic activity and fatty acid production. This discovery opens up new possibilities for developing selective ACC inhibitors to combat diseases linked to metabolic syndrome.
A team of researchers used computational simulations to gain insights into how an enzyme activates and shuts off phospholipid production. The study's results could help understand why small changes in the enzyme lead to conditions like blindness and dwarfism.
Researchers have identified key genes involved in enzymatic degradation of sugarcane biomass by three fungal species. The study, supported by FAPESP, provides insights into the genetic mechanisms controlling enzyme secretion and expression, paving the way for more efficient biomass breakdown and production of biofuels.
Stanford researchers engineered yeast to produce noscapine, a promising cancer drug with less toxicity than current treatments. The breakthrough involves introducing 25 foreign genes into yeast, achieving an 18,000-fold improvement in noscapine output.
Scientists at Lawrence Berkeley National Laboratory and JBEI have discovered a new enzyme that enables microbial production of toluene, an aromatic biofuel. The discovery is a major breakthrough in biotechnology, expanding the known catalytic range of glycyl radical enzymes and opening up new possibilities for renewable energy.
Researchers at the University of Kent and Bristol have built a miniature scaffold inside bacteria, enabling efficient production of chemicals and biofuels. The technology, which can increase alcohol production by over 200%, has significant implications for the next generation of biofuel production.
Researchers have identified the enzymes responsible for psilocybin production in magic mushrooms, which could lead to its biotechnological production. The study reveals a previously unknown biosynthetic pathway and introduces a synthetic route that could transform psilocybin into a pharmaceutical ingredient.